Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">N- and L-type calcium channel involvement in depolarization-induced suppression of inhibition in rat hippocampal CA1 cells</title><author><name sortKey="Lenz, R A" sort="Lenz, R A" uniqKey="Lenz R" first="R A" last="Lenz">R A Lenz</name></author><author><name sortKey="Wagner, J J" sort="Wagner, J J" uniqKey="Wagner J" first="J J" last="Wagner">J J Wagner</name></author><author><name sortKey="Alger, B E" sort="Alger, B E" uniqKey="Alger B" first="B E" last="Alger">B E Alger</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">9729617</idno><idno type="pmc">2231194</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2231194</idno><idno type="RBID">PMC:2231194</idno><idno type="doi">10.1111/j.1469-7793.1998.061bf.x</idno><date when="1998">1998</date><idno type="wicri:Area/Pmc/Corpus">000318</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000318</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">N- and L-type calcium channel involvement in depolarization-induced suppression of inhibition in rat hippocampal CA1 cells</title><author><name sortKey="Lenz, R A" sort="Lenz, R A" uniqKey="Lenz R" first="R A" last="Lenz">R A Lenz</name></author><author><name sortKey="Wagner, J J" sort="Wagner, J J" uniqKey="Wagner J" first="J J" last="Wagner">J J Wagner</name></author><author><name sortKey="Alger, B E" sort="Alger, B E" uniqKey="Alger B" first="B E" last="Alger">B E Alger</name></author></analytic><series><title level="j">The Journal of Physiology</title><idno type="ISSN">0022-3751</idno><idno type="eISSN">1469-7793</idno><imprint><date when="1998">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><list list-type="order"><list-item><p>We investigated depolarization-induced suppression of inhibition (DSI) under whole-cell voltage clamp in CA1 pyramidal neurons of rat hippocampal slices. DSI, a transient reduction in monosynaptic evoked GABA<sub>A</sub>ergic IPSCs lasting for ∼1 min, was induced by depolarizing the pyramidal cell to −10 or 0 mV for 1 or 2 s.</p></list-item><list-item><p>Raising extracellular Ca<sup>2+</sup> concentration increased DSI, and varying the DSI-inducing voltage step showed that the voltage dependence of DSI was like that of high-voltage-activated Ca<sup>2+</sup> channels.</p></list-item><list-item><p>The P- and Q-type Ca<sup>2+</sup> channel blocker ω-agatoxin TK (200 n<sc>m</sc> and 1 μ<sc>m</sc>) and the R- and T-type Ca<sup>2+</sup> channel blocker Ni<sup>2+</sup> (100 μ<sc>m</sc>) reduced IPSCs without reducing DSI.</p></list-item><list-item><p>The specific N-type Ca<sup>2+</sup> channel antagonist ω-conotoxin GVIA (250 n<sc>m</sc>) reduced IPSC amplitudes and almost completely abolished DSI.</p></list-item><list-item><p>Blocking L-type Ca<sup>2+</sup> channels with nifedipine (10 μ<sc>m</sc>) had no effect on IPSCs or DSI induced by our standard protocol, but reduced DSI induced by the unclamped Na<sup>+</sup>- and Ca<sup>2+</sup>-dependent spikes that occurred when 2(triethylamino)-<italic>N-</italic>(2,6-dimethylphenyl)acetamide (QX-314) was omitted from the recording pipette solution.</p></list-item><list-item><p>Although intracellular Ca<sup>2+</sup> stores were not measured, DSI was not affected by cyclopiazonic acid (CPA, 20–40 μ<sc>m</sc>), a blocker of Ca<sup>2+</sup> uptake into intracellular stores.</p></list-item><list-item><p>We conclude that DSI is initiated by Ca<sup>2+</sup> influx through N- and, under certain conditions, L-type Ca<sup>2+</sup> channels.</p></list-item></list></p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Physiol</journal-id><journal-id journal-id-type="publisher-id">tjp</journal-id><journal-title>The Journal of Physiology</journal-title><issn pub-type="ppub">0022-3751</issn><issn pub-type="epub">1469-7793</issn><publisher><publisher-name>Blackwell Science Inc</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">9729617</article-id><article-id pub-id-type="pmc">2231194</article-id><article-id pub-id-type="doi">10.1111/j.1469-7793.1998.061bf.x</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject></subj-group></article-categories><title-group><article-title>N- and L-type calcium channel involvement in depolarization-induced suppression of inhibition in rat hippocampal CA1 cells</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lenz</surname><given-names>R A</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wagner</surname><given-names>J J</given-names></name></contrib><contrib contrib-type="author"><name><surname>Alger</surname><given-names>B E</given-names></name></contrib><aff><institution>Department of Physiology, University of Maryland School of Medicine</institution><addr-line>Baltimore, MD 21201, USA</addr-line></aff></contrib-group><author-notes><corresp id="cor1"><bold>Corresponding author</bold> B. E. Alger: Department of Physiology, University of Maryland School of Medicine, 655 West Baltimore Street, Baltimore, MD 21201, USA. Email: <email>balger@umaryland.edu</email></corresp></author-notes><pub-date pub-type="ppub"><day>01</day><month>10</month><year>1998</year></pub-date><volume>512</volume><issue>Pt 1</issue><fpage>61</fpage><lpage>73</lpage><history><date date-type="received"><day>02</day><month>3</month><year>1998</year></date><date date-type="accepted"><day>25</day><month>6</month><year>1998</year></date></history><copyright-statement>© The Physiological Society 1998</copyright-statement><copyright-year>1998</copyright-year><abstract><p><list list-type="order"><list-item><p>We investigated depolarization-induced suppression of inhibition (DSI) under whole-cell voltage clamp in CA1 pyramidal neurons of rat hippocampal slices. DSI, a transient reduction in monosynaptic evoked GABA<sub>A</sub>ergic IPSCs lasting for ∼1 min, was induced by depolarizing the pyramidal cell to −10 or 0 mV for 1 or 2 s.</p></list-item><list-item><p>Raising extracellular Ca<sup>2+</sup> concentration increased DSI, and varying the DSI-inducing voltage step showed that the voltage dependence of DSI was like that of high-voltage-activated Ca<sup>2+</sup> channels.</p></list-item><list-item><p>The P- and Q-type Ca<sup>2+</sup> channel blocker ω-agatoxin TK (200 n<sc>m</sc> and 1 μ<sc>m</sc>) and the R- and T-type Ca<sup>2+</sup> channel blocker Ni<sup>2+</sup> (100 μ<sc>m</sc>) reduced IPSCs without reducing DSI.</p></list-item><list-item><p>The specific N-type Ca<sup>2+</sup> channel antagonist ω-conotoxin GVIA (250 n<sc>m</sc>) reduced IPSC amplitudes and almost completely abolished DSI.</p></list-item><list-item><p>Blocking L-type Ca<sup>2+</sup> channels with nifedipine (10 μ<sc>m</sc>) had no effect on IPSCs or DSI induced by our standard protocol, but reduced DSI induced by the unclamped Na<sup>+</sup>- and Ca<sup>2+</sup>-dependent spikes that occurred when 2(triethylamino)-<italic>N-</italic>(2,6-dimethylphenyl)acetamide (QX-314) was omitted from the recording pipette solution.</p></list-item><list-item><p>Although intracellular Ca<sup>2+</sup> stores were not measured, DSI was not affected by cyclopiazonic acid (CPA, 20–40 μ<sc>m</sc>), a blocker of Ca<sup>2+</sup> uptake into intracellular stores.</p></list-item><list-item><p>We conclude that DSI is initiated by Ca<sup>2+</sup> influx through N- and, under certain conditions, L-type Ca<sup>2+</sup> channels.</p></list-item></list></p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 0003180 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 0003180 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= CobaltMaghrebV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.32. |  |