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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Properties of mixtures of cholesterol with phosphatidylcholine or with phosphatidylserine studied by (13)C magic angle spinning nuclear magnetic resonance.</title><author><name sortKey="Epand, Richard M" sort="Epand, Richard M" uniqKey="Epand R" first="Richard M" last="Epand">Richard M. Epand</name></author><author><name sortKey="Bain, Alex D" sort="Bain, Alex D" uniqKey="Bain A" first="Alex D" last="Bain">Alex D. Bain</name></author><author><name sortKey="Sayer, Brian G" sort="Sayer, Brian G" uniqKey="Sayer B" first="Brian G" last="Sayer">Brian G. Sayer</name></author><author><name sortKey="Bach, Diana" sort="Bach, Diana" uniqKey="Bach D" first="Diana" last="Bach">Diana Bach</name></author><author><name sortKey="Wachtel, Ellen" sort="Wachtel, Ellen" uniqKey="Wachtel E" first="Ellen" last="Wachtel">Ellen Wachtel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12324423</idno><idno type="pmc">1302294</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1302294</idno><idno type="RBID">PMC:1302294</idno><date when="2002">2002</date><idno type="wicri:Area/Pmc/Corpus">000155</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000155</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Properties of mixtures of cholesterol with phosphatidylcholine or with phosphatidylserine studied by (13)C magic angle spinning nuclear magnetic resonance.</title><author><name sortKey="Epand, Richard M" sort="Epand, Richard M" uniqKey="Epand R" first="Richard M" last="Epand">Richard M. Epand</name></author><author><name sortKey="Bain, Alex D" sort="Bain, Alex D" uniqKey="Bain A" first="Alex D" last="Bain">Alex D. Bain</name></author><author><name sortKey="Sayer, Brian G" sort="Sayer, Brian G" uniqKey="Sayer B" first="Brian G" last="Sayer">Brian G. Sayer</name></author><author><name sortKey="Bach, Diana" sort="Bach, Diana" uniqKey="Bach D" first="Diana" last="Bach">Diana Bach</name></author><author><name sortKey="Wachtel, Ellen" sort="Wachtel, Ellen" uniqKey="Wachtel E" first="Ellen" last="Wachtel">Ellen Wachtel</name></author></analytic><series><title level="j">Biophysical Journal</title><idno type="ISSN">0006-3495</idno><idno type="eISSN">1542-0086</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The behavior of cholesterol is different in mixtures with phosphatidylcholine as compared with phosphatidylserine. In (13)C cross polarization/magic angle spinning nuclear magnetic resonance spectra, resonance peaks of the vinylic carbons of cholesterol are a doublet in samples containing 0.3 or 0.5 mol fraction cholesterol with 1-palmitoyl-2-oleoyl phosphatidylserine (POPS) or in cholesterol monohydrate crystals, but a singlet with mixtures of cholesterol and 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC). At these molar fractions of cholesterol with POPS, resonances of the C-18 of cholesterol appear at the same chemical shifts as in pure cholesterol monohydrate crystals. These resonances do not appear in samples of POPS with 0.2 mol fraction cholesterol or with POPC up to 0.5 mol fraction cholesterol. In addition, there is another resonance from the cholesterol C18 that appears in all of the mixtures of phospholipid and cholesterol but not in pure cholesterol monohydrate crystals. Using direct polarization, the fraction of cholesterol present as crystallites in POPS with 0.5 mol fraction cholesterol is found to be 80%, whereas with the same mol fraction of cholesterol and POPC none of the cholesterol is crystalline. After many hours of incubation, cholesterol monohydrate crystals in POPS undergo a change that results in an increase in the intensity of certain resonances of cholesterol monohydrate in (13)C cross polarization/magic angle spinning nuclear magnetic resonance, indicating a rigidification of the C and D rings of cholesterol but not other regions of the molecule.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biophys J</journal-id><journal-title>Biophysical Journal</journal-title><issn pub-type="ppub">0006-3495</issn><issn pub-type="epub">1542-0086</issn></journal-meta><article-meta><article-id pub-id-type="pmid">12324423</article-id><article-id pub-id-type="pmc">1302294</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Properties of mixtures of cholesterol with phosphatidylcholine or with phosphatidylserine studied by (13)C magic angle spinning nuclear magnetic resonance.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Epand</surname><given-names>Richard M</given-names></name></contrib><contrib contrib-type="author"><name><surname>Bain</surname><given-names>Alex D</given-names></name></contrib><contrib contrib-type="author"><name><surname>Sayer</surname><given-names>Brian G</given-names></name></contrib><contrib contrib-type="author"><name><surname>Bach</surname><given-names>Diana</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wachtel</surname><given-names>Ellen</given-names></name></contrib></contrib-group><aff>Department of Biochemistry, McMaster University, Hamilton, Ontario L8S 4M1, Canada. epand@mcmaster.ca</aff><pub-date pub-type="ppub"><month>10</month><year>2002</year></pub-date><volume>83</volume><issue>4</issue><fpage>2053</fpage><lpage>2063</lpage><abstract><p>The behavior of cholesterol is different in mixtures with phosphatidylcholine as compared with phosphatidylserine. In (13)C cross polarization/magic angle spinning nuclear magnetic resonance spectra, resonance peaks of the vinylic carbons of cholesterol are a doublet in samples containing 0.3 or 0.5 mol fraction cholesterol with 1-palmitoyl-2-oleoyl phosphatidylserine (POPS) or in cholesterol monohydrate crystals, but a singlet with mixtures of cholesterol and 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC). At these molar fractions of cholesterol with POPS, resonances of the C-18 of cholesterol appear at the same chemical shifts as in pure cholesterol monohydrate crystals. These resonances do not appear in samples of POPS with 0.2 mol fraction cholesterol or with POPC up to 0.5 mol fraction cholesterol. In addition, there is another resonance from the cholesterol C18 that appears in all of the mixtures of phospholipid and cholesterol but not in pure cholesterol monohydrate crystals. Using direct polarization, the fraction of cholesterol present as crystallites in POPS with 0.5 mol fraction cholesterol is found to be 80%, whereas with the same mol fraction of cholesterol and POPC none of the cholesterol is crystalline. After many hours of incubation, cholesterol monohydrate crystals in POPS undergo a change that results in an increase in the intensity of certain resonances of cholesterol monohydrate in (13)C cross polarization/magic angle spinning nuclear magnetic resonance, indicating a rigidification of the C and D rings of cholesterol but not other regions of the molecule.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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