Exploring antibiotic resistance genes and metal resistance genes in plasmid metagenomes from wastewater treatment plants
Identifieur interne : 000081 ( Pmc/Checkpoint ); précédent : 000080; suivant : 000082Exploring antibiotic resistance genes and metal resistance genes in plasmid metagenomes from wastewater treatment plants
Auteurs : An-Dong Li ; Li-Guan Li ; Tong ZhangSource :
- Frontiers in Microbiology [ 1664-302X ] ; 2015.
Abstract
Plasmids operate as independent genetic elements in microorganism communities. Through horizontal gene transfer (HGT), they can provide their host microorganisms with important functions such as antibiotic resistance and heavy metal resistance. In this study, six metagenomic libraries were constructed with plasmid DNA extracted from influent, activated sludge (AS) and digested sludge (DS) of two wastewater treatment plants (WWTPs). Compared with the metagenomes of the total DNA extracted from the same sectors of the wastewater treatment plant, the plasmid metagenomes had significantly higher annotation rates, indicating that the functional genes on plasmids are commonly shared by those studied microorganisms. Meanwhile, the plasmid metagenomes also encoded many more genes related to defense mechanisms, including ARGs. Searching against an antibiotic resistance genes (ARGs) database and a metal resistance genes (MRGs) database revealed a broad-spectrum of antibiotic (323 out of a total 618 subtypes) and MRGs (23 out of a total 23 types) on these plasmid metagenomes. The influent plasmid metagenomes contained many more resistance genes (both ARGs and MRGs) than the AS and the DS metagenomes. Sixteen novel plasmids with a complete circular structure that carried these resistance genes were assembled from the plasmid metagenomes. The results of this study demonstrated that the plasmids in WWTPs could be important reservoirs for resistance genes, and may play a significant role in the horizontal transfer of these genes.
Url:
DOI: 10.3389/fmicb.2015.01025
PubMed: 26441947
PubMed Central: 4585309
Affiliations:
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Exploring antibiotic resistance genes and metal resistance genes in plasmid metagenomes from wastewater treatment plants</title>
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<front><div type="abstract" xml:lang="en"><p>Plasmids operate as independent genetic elements in microorganism communities. Through horizontal gene transfer (HGT), they can provide their host microorganisms with important functions such as antibiotic resistance and heavy metal resistance. In this study, six metagenomic libraries were constructed with plasmid DNA extracted from influent, activated sludge (AS) and digested sludge (DS) of two wastewater treatment plants (WWTPs). Compared with the metagenomes of the total DNA extracted from the same sectors of the wastewater treatment plant, the plasmid metagenomes had significantly higher annotation rates, indicating that the functional genes on plasmids are commonly shared by those studied microorganisms. Meanwhile, the plasmid metagenomes also encoded many more genes related to defense mechanisms, including ARGs. Searching against an antibiotic resistance genes (ARGs) database and a metal resistance genes (MRGs) database revealed a broad-spectrum of antibiotic (323 out of a total 618 subtypes) and MRGs (23 out of a total 23 types) on these plasmid metagenomes. The influent plasmid metagenomes contained many more resistance genes (both ARGs and MRGs) than the AS and the DS metagenomes. Sixteen novel plasmids with a complete circular structure that carried these resistance genes were assembled from the plasmid metagenomes. The results of this study demonstrated that the plasmids in WWTPs could be important reservoirs for resistance genes, and may play a significant role in the horizontal transfer of these genes.</p>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Microbiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Front Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">Front. Microbiol.</journal-id>
<journal-title-group><journal-title>Frontiers in Microbiology</journal-title>
</journal-title-group>
<issn pub-type="epub">1664-302X</issn>
<publisher><publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26441947</article-id>
<article-id pub-id-type="pmc">4585309</article-id>
<article-id pub-id-type="doi">10.3389/fmicb.2015.01025</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Microbiology</subject>
<subj-group><subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Exploring antibiotic resistance genes and metal resistance genes in plasmid metagenomes from wastewater treatment plants</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Li</surname>
<given-names>An-Dong</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Li-Guan</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Tong</given-names>
</name>
<xref ref-type="author-notes" rid="fn001"><sup>*</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/242364/overview"></uri>
</contrib>
</contrib-group>
<aff><institution>Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong</institution>
<country>Hong Kong, Hong Kong</country>
</aff>
<author-notes><fn fn-type="edited-by"><p>Edited by: Teresa M. Coque, Hospital Universitario Ramón y Cajal, Spain</p>
</fn>
<fn fn-type="edited-by"><p>Reviewed by: Eddie Cytryn, Agricultural Research Organization, Volcani Center, Israel; Fernando De La Cruz, Universidad de Cantabria, Spain; Carmen Losasso, Istituto Zooprofilattico Sperimentale delle Venezie, Italy; Jose L. Martinez, Centro Nacional de Biotecnología, Spain</p>
</fn>
<corresp id="fn001">*Correspondence: Tong Zhang, Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, Hong Kong <email xlink:type="simple">zhangt@hku.hk</email>
</corresp>
<fn fn-type="other" id="fn002"><p>This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>24</day>
<month>9</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="collection"><year>2015</year>
</pub-date>
<volume>6</volume>
<elocation-id>1025</elocation-id>
<history><date date-type="received"><day>04</day>
<month>6</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>09</day>
<month>9</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2015 Li, Li and Zhang.</copyright-statement>
<copyright-year>2015</copyright-year>
<copyright-holder>Li, Li and Zhang</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p>
</license>
</permissions>
<abstract><p>Plasmids operate as independent genetic elements in microorganism communities. Through horizontal gene transfer (HGT), they can provide their host microorganisms with important functions such as antibiotic resistance and heavy metal resistance. In this study, six metagenomic libraries were constructed with plasmid DNA extracted from influent, activated sludge (AS) and digested sludge (DS) of two wastewater treatment plants (WWTPs). Compared with the metagenomes of the total DNA extracted from the same sectors of the wastewater treatment plant, the plasmid metagenomes had significantly higher annotation rates, indicating that the functional genes on plasmids are commonly shared by those studied microorganisms. Meanwhile, the plasmid metagenomes also encoded many more genes related to defense mechanisms, including ARGs. Searching against an antibiotic resistance genes (ARGs) database and a metal resistance genes (MRGs) database revealed a broad-spectrum of antibiotic (323 out of a total 618 subtypes) and MRGs (23 out of a total 23 types) on these plasmid metagenomes. The influent plasmid metagenomes contained many more resistance genes (both ARGs and MRGs) than the AS and the DS metagenomes. Sixteen novel plasmids with a complete circular structure that carried these resistance genes were assembled from the plasmid metagenomes. The results of this study demonstrated that the plasmids in WWTPs could be important reservoirs for resistance genes, and may play a significant role in the horizontal transfer of these genes.</p>
</abstract>
<kwd-group><kwd>plasmid</kwd>
<kwd>wastewater treatment</kwd>
<kwd>antibiotic resistance</kwd>
<kwd>metal resistance</kwd>
<kwd>metagenome</kwd>
</kwd-group>
<counts><fig-count count="5"></fig-count>
<table-count count="1"></table-count>
<equation-count count="0"></equation-count>
<ref-count count="53"></ref-count>
<page-count count="11"></page-count>
<word-count count="7031"></word-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Li, An Dong" sort="Li, An Dong" uniqKey="Li A" first="An-Dong" last="Li">An-Dong Li</name>
<name sortKey="Li, Li Guan" sort="Li, Li Guan" uniqKey="Li L" first="Li-Guan" last="Li">Li-Guan Li</name>
<name sortKey="Zhang, Tong" sort="Zhang, Tong" uniqKey="Zhang T" first="Tong" last="Zhang">Tong Zhang</name>
</noCountry>
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</affiliations>
</record>
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