CobaltMaghrebV1, PascalFrancis, Curation, bibRecord, 000097

A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster

Identifieur interne : 000097 ( PascalFrancis/Curation ); précédent : 000096; suivant : 000098

A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster

Auteurs : A. Crew [Royaume-Uni] ; J. P. Hart [Royaume-Uni] ; R. Wedge [Royaume-Uni] ; J. L. Marty [France] ; D. Fournier [France]

Source :

Analytical letters [ 0003-2719 ] ; 2004.

RBID : Pascal:05-0048689

Descripteurs français

Pascal (Inist)
- Ampérométrie, Biodétecteur, Inhibition, Electrode carbone, Transducteur, Enzyme, Chronoampérométrie, Etalonnage, Industrie alimentaire, Produit alimentaire, Blé, Pomme, Préparation échantillon, Capteur chimique, Pesticide, Acetylcholinesterase, Cobalt, Phtalocyanine, Plomb, Dibenzo-p-dioxine(polychloro), Chlorpyriphos, Malaoxon.
Wicri :
- topic : Enzyme, Industrie alimentaire, Produit alimentaire, Blé, Pesticide, Cobalt, Plomb.

English descriptors

KwdEn :
- Acetylcholinesterase, Amperometry, Apple, Biosensor, Calibration, Carbon electrode, Chemical sensor, Chlorpyrifos, Chronoamperometry, Cobalt, Enzyme, Food industry, Foodstuff, Inhibition, Lead, Pesticides, Phthalocyanine, Sample preparation, Transducer, Wheat.

Abstract

Screen-printed carbon electrodes (SPCEs) modified with cobalt phthalocyanine (CoPC) have been used as base transducers in the construction of amperometric pesticide biosensors. Six individual biosensors were fabricated by depositing wildtype (WT) acetylcholinesterase (AChE) from Drosophila melanogaster or one of five mutant forms (B02, B03, B04, B421, and B65) of this enzyme, onto the surfaces of CoPC-SPCEs; these constituted the amperometric biosensor array. The enzyme converts acetylthiocholine into its electroactive product thiocholine. which is detected at only 0V vs. Ag/AgCl at the CoPC-SPCEs. The measurement step is performed using chronoamperometry. In the presence of an organophosphate (OP) pesticide, the enzyme is inhibited, which leads to a decrease in thiocholine production and a corresponding decrease in anodic current. This decrease is proportional to the logarithm of the pesticide concentration. Calibration studies were performed with the biosensor array using five OPs of interest to the food industry, namely omethoate, malaoxon, dichlorvos, chlorpyrifos-methyl-oxon, and pirimiphosmethyl-oxon. It was found that different inhibition patterns occurred for the five OPs indicating the possibility of identifying and quantifying these compounds in food samples. It should be added that there was no detrimental affect on the biosensor response from wheat or apple extracts.

A01	`01`	`1`		`@0 0003-2719`
A02	`01`			`@0 ANALBP`
A03		`1`		`@0 Anal. lett.`
A05				`@2 37`
A06				`@2 8`
A08	`01`	`1`	`ENG`	`@1 A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster`
A09	`01`	`1`	`ENG`	`@1 Selected papers from the international meeting on biosensors for food safety and environmental monitoring`
A11	`01`	`1`		`@1 CREW (A.)`
A11	`02`	`1`		`@1 HART (J. P.)`
A11	`03`	`1`		`@1 WEDGE (R.)`
A11	`04`	`1`		`@1 MARTY (J. L.)`
A11	`05`	`1`		`@1 FOURNIER (D.)`
A12	`01`	`1`		`@1 AMINE (Aziz) @9 ed.`
A12	`02`	`1`		`@1 BRETT (Christopher) @9 ed.`
A12	`03`	`1`		`@1 PALLESCHI (Giuseppe) @9 ed.`
A14	`01`			`@1 CRAMSS, Faculty of Applied Sciences, University of the West of England, Coldharbour Lane @2 Frenchay, Bristol @3 GBR @Z 1 aut. @Z 2 aut. @Z 3 aut.`
A14	`02`			`@1 Centre de Phytopharmacie, Université de Perpignan @2 Perpignan @3 FRA @Z 4 aut.`
A14	`03`			`@1 Laboratoire de Synthèse et Phytochimie des Molécules d'Intérêt Biologique, Université Paul Sabatier @2 Toulouse @3 FRA @Z 5 aut.`
A15	`01`			`@1 University of Hassan II-Mohammedia @2 Mohammedia @3 MAR @Z 1 aut.`
A15	`02`			`@1 University of Coimbra @2 Coimbra @3 PRT @Z 2 aut.`
A15	`03`			`@1 University of Rome Tor Vergata @2 Rome @3 ITA @Z 3 aut.`
A20				`@1 1601-1610`
A21				`@1 2004`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 13703 @5 354000113734620090`
A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
A45				`@0 12 ref.`
A47	`01`	`1`		`@0 05-0048689`
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A61				`@0 A`
A64	`01`	`1`		`@0 Analytical letters`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 Screen-printed carbon electrodes (SPCEs) modified with cobalt phthalocyanine (CoPC) have been used as base transducers in the construction of amperometric pesticide biosensors. Six individual biosensors were fabricated by depositing wildtype (WT) acetylcholinesterase (AChE) from Drosophila melanogaster or one of five mutant forms (B02, B03, B04, B421, and B65) of this enzyme, onto the surfaces of CoPC-SPCEs; these constituted the amperometric biosensor array. The enzyme converts acetylthiocholine into its electroactive product thiocholine. which is detected at only 0V vs. Ag/AgCl at the CoPC-SPCEs. The measurement step is performed using chronoamperometry. In the presence of an organophosphate (OP) pesticide, the enzyme is inhibited, which leads to a decrease in thiocholine production and a corresponding decrease in anodic current. This decrease is proportional to the logarithm of the pesticide concentration. Calibration studies were performed with the biosensor array using five OPs of interest to the food industry, namely omethoate, malaoxon, dichlorvos, chlorpyrifos-methyl-oxon, and pirimiphosmethyl-oxon. It was found that different inhibition patterns occurred for the five OPs indicating the possibility of identifying and quantifying these compounds in food samples. It should be added that there was no detrimental affect on the biosensor response from wheat or apple extracts.
C02	`01`	`X`		`@0 001C04E`
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C02	`03`	`X`		`@0 001C04A`
C02	`04`	`X`		`@0 215`
C03	`01`	`X`	`FRE`	`@0 Ampérométrie @5 01`
C03	`01`	`X`	`ENG`	`@0 Amperometry @5 01`
C03	`01`	`X`	`SPA`	`@0 Amperometría @5 01`
C03	`02`	`X`	`FRE`	`@0 Biodétecteur @5 02`
C03	`02`	`X`	`ENG`	`@0 Biosensor @5 02`
C03	`02`	`X`	`SPA`	`@0 Biodetector @5 02`
C03	`03`	`X`	`FRE`	`@0 Inhibition @5 03`
C03	`03`	`X`	`ENG`	`@0 Inhibition @5 03`
C03	`03`	`X`	`SPA`	`@0 Inhibición @5 03`
C03	`04`	`X`	`FRE`	`@0 Electrode carbone @5 04`
C03	`04`	`X`	`ENG`	`@0 Carbon electrode @5 04`
C03	`04`	`X`	`SPA`	`@0 Electrodo carbono @5 04`
C03	`05`	`X`	`FRE`	`@0 Transducteur @5 05`
C03	`05`	`X`	`ENG`	`@0 Transducer @5 05`
C03	`05`	`X`	`SPA`	`@0 Transductor @5 05`
C03	`06`	`X`	`FRE`	`@0 Enzyme @2 FE @5 06`
C03	`06`	`X`	`ENG`	`@0 Enzyme @2 FE @5 06`
C03	`06`	`X`	`SPA`	`@0 Enzima @2 FE @5 06`
C03	`07`	`X`	`FRE`	`@0 Chronoampérométrie @5 07`
C03	`07`	`X`	`ENG`	`@0 Chronoamperometry @5 07`
C03	`07`	`X`	`SPA`	`@0 Cronoamperimetría @5 07`
C03	`08`	`X`	`FRE`	`@0 Etalonnage @5 08`
C03	`08`	`X`	`ENG`	`@0 Calibration @5 08`
C03	`08`	`X`	`SPA`	`@0 Contraste @5 08`
C03	`09`	`X`	`FRE`	`@0 Industrie alimentaire @5 09`
C03	`09`	`X`	`ENG`	`@0 Food industry @5 09`
C03	`09`	`X`	`SPA`	`@0 Industria alimenticia @5 09`
C03	`10`	`X`	`FRE`	`@0 Produit alimentaire @5 10`
C03	`10`	`X`	`ENG`	`@0 Foodstuff @5 10`
C03	`10`	`X`	`SPA`	`@0 Producto alimenticio @5 10`
C03	`11`	`X`	`FRE`	`@0 Blé @5 11`
C03	`11`	`X`	`ENG`	`@0 Wheat @5 11`
C03	`11`	`X`	`SPA`	`@0 Trigo @5 11`
C03	`12`	`X`	`FRE`	`@0 Pomme @5 12`
C03	`12`	`X`	`ENG`	`@0 Apple @5 12`
C03	`12`	`X`	`SPA`	`@0 Manzana @5 12`
C03	`13`	`X`	`FRE`	`@0 Préparation échantillon @5 13`
C03	`13`	`X`	`ENG`	`@0 Sample preparation @5 13`
C03	`13`	`X`	`SPA`	`@0 Preparación muestreo @5 13`
C03	`14`	`X`	`FRE`	`@0 Capteur chimique @5 14`
C03	`14`	`X`	`ENG`	`@0 Chemical sensor @5 14`
C03	`14`	`X`	`SPA`	`@0 Captador químico @5 14`
C03	`15`	`X`	`FRE`	`@0 Pesticide @5 15`
C03	`15`	`X`	`ENG`	`@0 Pesticides @5 15`
C03	`15`	`X`	`SPA`	`@0 Plaguicida @5 15`
C03	`16`	`X`	`FRE`	`@0 Acetylcholinesterase @2 FE @5 16`
C03	`16`	`X`	`ENG`	`@0 Acetylcholinesterase @2 FE @5 16`
C03	`16`	`X`	`SPA`	`@0 Acetylcholinesterase @2 FE @5 16`
C03	`17`	`X`	`FRE`	`@0 Cobalt @2 NC @5 17`
C03	`17`	`X`	`ENG`	`@0 Cobalt @2 NC @5 17`
C03	`17`	`X`	`SPA`	`@0 Cobalto @2 NC @5 17`
C03	`18`	`X`	`FRE`	`@0 Phtalocyanine @2 NK @5 18`
C03	`18`	`X`	`ENG`	`@0 Phthalocyanine @2 NK @5 18`
C03	`18`	`X`	`SPA`	`@0 Ftalocianina @2 NK @5 18`
C03	`19`	`X`	`FRE`	`@0 Plomb @2 NC @2 FX @5 19`
C03	`19`	`X`	`ENG`	`@0 Lead @2 NC @2 FX @5 19`
C03	`19`	`X`	`SPA`	`@0 Plomo @2 NC @2 FX @5 19`
C03	`20`	`X`	`FRE`	`@0 Dibenzo-p-dioxine(polychloro) @2 NK @2 FX @5 20 @6 Dibenzo-«p»-dioxine(polychloro)`
C03	`21`	`X`	`FRE`	`@0 Chlorpyriphos @2 NK @2 FF @5 21`
C03	`21`	`X`	`ENG`	`@0 Chlorpyrifos @2 NK @2 FF @5 21`
C03	`21`	`X`	`SPA`	`@0 Clorpirifos @2 NK @2 FF @5 21`
C03	`22`	`X`	`FRE`	`@0 Malaoxon @4 INC @5 32`
C07	`01`	`X`	`FRE`	`@0 Carboxylic ester hydrolases @2 FE`
C07	`01`	`X`	`ENG`	`@0 Carboxylic ester hydrolases @2 FE`
C07	`01`	`X`	`SPA`	`@0 Carboxylic ester hydrolases @2 FE`
C07	`02`	`X`	`FRE`	`@0 Esterases @2 FE`
C07	`02`	`X`	`ENG`	`@0 Esterases @2 FE`
C07	`02`	`X`	`SPA`	`@0 Esterases @2 FE`
C07	`03`	`X`	`FRE`	`@0 Hydrolases @2 FE`
C07	`03`	`X`	`ENG`	`@0 Hydrolases @2 FE`
C07	`03`	`X`	`SPA`	`@0 Hydrolases @2 FE`
N21				`@1 024`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

A30	`01`	`1`	`ENG`	`@1 Biosensors for Food Safety and Environmental Monitoring. International Meeting @3 Marrakech MAR @4 2003-10-09`

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Pascal:05-0048689

Le document en format XML

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<author><name sortKey="Hart, J P" sort="Hart, J P" uniqKey="Hart J" first="J. P." last="Hart">J. P. Hart</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetylcholinesterase</term>
<term>Amperometry</term>
<term>Apple</term>
<term>Biosensor</term>
<term>Calibration</term>
<term>Carbon electrode</term>
<term>Chemical sensor</term>
<term>Chlorpyrifos</term>
<term>Chronoamperometry</term>
<term>Cobalt</term>
<term>Enzyme</term>
<term>Food industry</term>
<term>Foodstuff</term>
<term>Inhibition</term>
<term>Lead</term>
<term>Pesticides</term>
<term>Phthalocyanine</term>
<term>Sample preparation</term>
<term>Transducer</term>
<term>Wheat</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Ampérométrie</term>
<term>Biodétecteur</term>
<term>Inhibition</term>
<term>Electrode carbone</term>
<term>Transducteur</term>
<term>Enzyme</term>
<term>Chronoampérométrie</term>
<term>Etalonnage</term>
<term>Industrie alimentaire</term>
<term>Produit alimentaire</term>
<term>Blé</term>
<term>Pomme</term>
<term>Préparation échantillon</term>
<term>Capteur chimique</term>
<term>Pesticide</term>
<term>Acetylcholinesterase</term>
<term>Cobalt</term>
<term>Phtalocyanine</term>
<term>Plomb</term>
<term>Dibenzo-p-dioxine(polychloro)</term>
<term>Chlorpyriphos</term>
<term>Malaoxon</term>
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<term>Industrie alimentaire</term>
<term>Produit alimentaire</term>
<term>Blé</term>
<term>Pesticide</term>
<term>Cobalt</term>
<term>Plomb</term>
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<front><div type="abstract" xml:lang="en">Screen-printed carbon electrodes (SPCEs) modified with cobalt phthalocyanine (CoPC) have been used as base transducers in the construction of amperometric pesticide biosensors. Six individual biosensors were fabricated by depositing wildtype (WT) acetylcholinesterase (AChE) from Drosophila melanogaster or one of five mutant forms (B02, B03, B04, B421, and B65) of this enzyme, onto the surfaces of CoPC-SPCEs; these constituted the amperometric biosensor array. The enzyme converts acetylthiocholine into its electroactive product thiocholine. which is detected at only 0V vs. Ag/AgCl at the CoPC-SPCEs. The measurement step is performed using chronoamperometry. In the presence of an organophosphate (OP) pesticide, the enzyme is inhibited, which leads to a decrease in thiocholine production and a corresponding decrease in anodic current. This decrease is proportional to the logarithm of the pesticide concentration. Calibration studies were performed with the biosensor array using five OPs of interest to the food industry, namely omethoate, malaoxon, dichlorvos, chlorpyrifos-methyl-oxon, and pirimiphosmethyl-oxon. It was found that different inhibition patterns occurred for the five OPs indicating the possibility of identifying and quantifying these compounds in food samples. It should be added that there was no detrimental affect on the biosensor response from wheat or apple extracts.</div>
</front>
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<fA08 i1="01" i2="1" l="ENG"><s1>A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster</s1>
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</fA11>
<fA12 i1="01" i2="1"><s1>AMINE (Aziz)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="02" i2="1"><s1>BRETT (Christopher)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="03" i2="1"><s1>PALLESCHI (Giuseppe)</s1>
<s9>ed.</s9>
</fA12>
<fA14 i1="01"><s1>CRAMSS, Faculty of Applied Sciences, University of the West of England, Coldharbour Lane</s1>
<s2>Frenchay, Bristol</s2>
<s3>GBR</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Centre de Phytopharmacie, Université de Perpignan</s1>
<s2>Perpignan</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Laboratoire de Synthèse et Phytochimie des Molécules d'Intérêt Biologique, Université Paul Sabatier</s1>
<s2>Toulouse</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA15 i1="01"><s1>University of Hassan II-Mohammedia</s1>
<s2>Mohammedia</s2>
<s3>MAR</s3>
<sZ>1 aut.</sZ>
</fA15>
<fA15 i1="02"><s1>University of Coimbra</s1>
<s2>Coimbra</s2>
<s3>PRT</s3>
<sZ>2 aut.</sZ>
</fA15>
<fA15 i1="03"><s1>University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>3 aut.</sZ>
</fA15>
<fA20><s1>1601-1610</s1>
</fA20>
<fA21><s1>2004</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>13703</s2>
<s5>354000113734620090</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>12 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>05-0048689</s0>
</fA47>
<fA60><s1>P</s1>
<s2>C</s2>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Analytical letters</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Screen-printed carbon electrodes (SPCEs) modified with cobalt phthalocyanine (CoPC) have been used as base transducers in the construction of amperometric pesticide biosensors. Six individual biosensors were fabricated by depositing wildtype (WT) acetylcholinesterase (AChE) from Drosophila melanogaster or one of five mutant forms (B02, B03, B04, B421, and B65) of this enzyme, onto the surfaces of CoPC-SPCEs; these constituted the amperometric biosensor array. The enzyme converts acetylthiocholine into its electroactive product thiocholine. which is detected at only 0V vs. Ag/AgCl at the CoPC-SPCEs. The measurement step is performed using chronoamperometry. In the presence of an organophosphate (OP) pesticide, the enzyme is inhibited, which leads to a decrease in thiocholine production and a corresponding decrease in anodic current. This decrease is proportional to the logarithm of the pesticide concentration. Calibration studies were performed with the biosensor array using five OPs of interest to the food industry, namely omethoate, malaoxon, dichlorvos, chlorpyrifos-methyl-oxon, and pirimiphosmethyl-oxon. It was found that different inhibition patterns occurred for the five OPs indicating the possibility of identifying and quantifying these compounds in food samples. It should be added that there was no detrimental affect on the biosensor response from wheat or apple extracts.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>001C04E</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A31C09B</s0>
</fC02>
<fC02 i1="03" i2="X"><s0>001C04A</s0>
</fC02>
<fC02 i1="04" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Ampérométrie</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Amperometry</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Amperometría</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Biodétecteur</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Biosensor</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Biodetector</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Inhibition</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Inhibition</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Inhibición</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Electrode carbone</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Carbon electrode</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Electrodo carbono</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Transducteur</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Transducer</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Transductor</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Chronoampérométrie</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Chronoamperometry</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Cronoamperimetría</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Etalonnage</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Calibration</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Contraste</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Industrie alimentaire</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Food industry</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Industria alimenticia</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Produit alimentaire</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Foodstuff</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Producto alimenticio</s0>
<s5>10</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Blé</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Wheat</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Trigo</s0>
<s5>11</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Pomme</s0>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Apple</s0>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Manzana</s0>
<s5>12</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Préparation échantillon</s0>
<s5>13</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Sample preparation</s0>
<s5>13</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Preparación muestreo</s0>
<s5>13</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Capteur chimique</s0>
<s5>14</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Chemical sensor</s0>
<s5>14</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Captador químico</s0>
<s5>14</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Pesticide</s0>
<s5>15</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG"><s0>Pesticides</s0>
<s5>15</s5>
</fC03>
<fC03 i1="15" i2="X" l="SPA"><s0>Plaguicida</s0>
<s5>15</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Acetylcholinesterase</s0>
<s2>FE</s2>
<s5>16</s5>
</fC03>
<fC03 i1="16" i2="X" l="ENG"><s0>Acetylcholinesterase</s0>
<s2>FE</s2>
<s5>16</s5>
</fC03>
<fC03 i1="16" i2="X" l="SPA"><s0>Acetylcholinesterase</s0>
<s2>FE</s2>
<s5>16</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Cobalt</s0>
<s2>NC</s2>
<s5>17</s5>
</fC03>
<fC03 i1="17" i2="X" l="ENG"><s0>Cobalt</s0>
<s2>NC</s2>
<s5>17</s5>
</fC03>
<fC03 i1="17" i2="X" l="SPA"><s0>Cobalto</s0>
<s2>NC</s2>
<s5>17</s5>
</fC03>
<fC03 i1="18" i2="X" l="FRE"><s0>Phtalocyanine</s0>
<s2>NK</s2>
<s5>18</s5>
</fC03>
<fC03 i1="18" i2="X" l="ENG"><s0>Phthalocyanine</s0>
<s2>NK</s2>
<s5>18</s5>
</fC03>
<fC03 i1="18" i2="X" l="SPA"><s0>Ftalocianina</s0>
<s2>NK</s2>
<s5>18</s5>
</fC03>
<fC03 i1="19" i2="X" l="FRE"><s0>Plomb</s0>
<s2>NC</s2>
<s2>FX</s2>
<s5>19</s5>
</fC03>
<fC03 i1="19" i2="X" l="ENG"><s0>Lead</s0>
<s2>NC</s2>
<s2>FX</s2>
<s5>19</s5>
</fC03>
<fC03 i1="19" i2="X" l="SPA"><s0>Plomo</s0>
<s2>NC</s2>
<s2>FX</s2>
<s5>19</s5>
</fC03>
<fC03 i1="20" i2="X" l="FRE"><s0>Dibenzo-p-dioxine(polychloro)</s0>
<s2>NK</s2>
<s2>FX</s2>
<s5>20</s5>
<s6>Dibenzo-«p»-dioxine(polychloro)</s6>
</fC03>
<fC03 i1="21" i2="X" l="FRE"><s0>Chlorpyriphos</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>21</s5>
</fC03>
<fC03 i1="21" i2="X" l="ENG"><s0>Chlorpyrifos</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>21</s5>
</fC03>
<fC03 i1="21" i2="X" l="SPA"><s0>Clorpirifos</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>21</s5>
</fC03>
<fC03 i1="22" i2="X" l="FRE"><s0>Malaoxon</s0>
<s4>INC</s4>
<s5>32</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Carboxylic ester hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Carboxylic ester hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Carboxylic ester hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Esterases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Esterases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Esterases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fN21><s1>024</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
<pR><fA30 i1="01" i2="1" l="ENG"><s1>Biosensors for Food Safety and Environmental Monitoring. International Meeting</s1>
<s3>Marrakech MAR</s3>
<s4>2003-10-09</s4>
</fA30>
</pR>
</standard>
</inist>
</record>

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A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster

A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster

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