CobaltMaghrebV1, PascalFrancis, Corpus, bibRecord, 000022

Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors

Identifieur interne : 000022 ( PascalFrancis/Corpus ); précédent : 000021; suivant : 000023

Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors

Auteurs : Najwa Ben Oujji ; Idriss Bakas ; Georges Istamboulie ; Ihya Ait-Ichou ; Elhabib Ait-Addi ; Regis Rouillon ; Thierry Noguer

Source :

Food control [ 0956-7135 ] ; 2013.

RBID : Pascal:13-0288738

Descripteurs français

Pascal (Inist)
- Procédé sol gel, Immobilisation, Organophosphoré, Pesticide, Huile olive, Biodétecteur, Anguille, Insecticide, Ampérométrie, Electrode.

English descriptors

KwdEn :
- Amperometry, Biosensor, Eel, Electrodes, Immobilization, Insecticide, Olive oil, Organophosphorus compounds, Pesticides, Sol gel process.

Abstract

This paper presents the construction of amperometric biosensors for the detection of organophosphorus insecticides widely used in the treatment of olive trees. The systems are based on the immobilisation of acetylcholinesterase on screen-printed electrodes by bioencapsulation in a sol-gel composite. The enzyme activity was estimated by measuring the thiocholine produced by the enzymatic hydrolysis of the acetylthiocholine chloride using cobalt phtalocyanine as mediator. The developed devices have been used to carry out inhibition studies with three pesticides: malathion, methidathion and dimethoate (in their oxidized form), and tested using standard solutions and real samples of olive oil. These biosensors showed good operational stability as they maintained their initial analytical signal response during 10 successive measurements, and a good reproducibility with a relative standard deviation of 3%. The limits of detection of the developed devices were very compatible with the maximum residue limit tolerated by international regulations, they were as low as 10^-9 M for the widely used pesticide malaoxon(oxidized malathion). The developed sensors were successfully used for the determinations of insecticides in real samples of olive oil.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A08	`01`	`1`	`ENG`	`@1 Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors`
A11	`01`	`1`		`@1 OUJJI (Najwa Ben)`
A11	`02`	`1`		`@1 BAKAS (Idriss)`
A11	`03`	`1`		`@1 ISTAMBOULIE (Georges)`
A11	`04`	`1`		`@1 AIT-ICHOU (Ihya)`
A11	`05`	`1`		`@1 AIT-ADDI (Elhabib)`
A11	`06`	`1`		`@1 ROUILLON (Regis)`
A11	`07`	`1`		`@1 NOGUER (Thierry)`
A14	`01`			`@1 University of Perpignan, IMAGES EA 4218, Building S 52 Av Paul Alduy @2 66860 Perpignan @3 FRA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 6 aut. @Z 7 aut.`
A14	`02`			`@1 University Ibn Zohr, AQUAMAR, Photocatalysis and Environment Team, Chemistry Department, Faculty of Science, BP 8106 Cité Dakhla @2 Agadir @3 MAR @Z 1 aut. @Z 2 aut. @Z 4 aut. @Z 5 aut.`
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A44				`@0 0000 @1 © 2013 INIST-CNRS. All rights reserved.`
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A61				`@0 A`
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C01	`01`		`ENG`	@0 This paper presents the construction of amperometric biosensors for the detection of organophosphorus insecticides widely used in the treatment of olive trees. The systems are based on the immobilisation of acetylcholinesterase on screen-printed electrodes by bioencapsulation in a sol-gel composite. The enzyme activity was estimated by measuring the thiocholine produced by the enzymatic hydrolysis of the acetylthiocholine chloride using cobalt phtalocyanine as mediator. The developed devices have been used to carry out inhibition studies with three pesticides: malathion, methidathion and dimethoate (in their oxidized form), and tested using standard solutions and real samples of olive oil. These biosensors showed good operational stability as they maintained their initial analytical signal response during 10 successive measurements, and a good reproducibility with a relative standard deviation of 3%. The limits of detection of the developed devices were very compatible with the maximum residue limit tolerated by international regulations, they were as low as 10^-9 M for the widely used pesticide malaoxon(oxidized malathion). The developed sensors were successfully used for the determinations of insecticides in real samples of olive oil.
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C02	`02`	`X`		`@0 002A31C09B`
C02	`03`	`X`		`@0 215`
C03	`01`	`X`	`FRE`	`@0 Procédé sol gel @5 01`
C03	`01`	`X`	`ENG`	`@0 Sol gel process @5 01`
C03	`01`	`X`	`SPA`	`@0 Procedimiento sol gel @5 01`
C03	`02`	`X`	`FRE`	`@0 Immobilisation @5 02`
C03	`02`	`X`	`ENG`	`@0 Immobilization @5 02`
C03	`02`	`X`	`SPA`	`@0 Inmovilización @5 02`
C03	`03`	`X`	`FRE`	`@0 Organophosphoré @5 10`
C03	`03`	`X`	`ENG`	`@0 Organophosphorus compounds @5 10`
C03	`03`	`X`	`SPA`	`@0 Organofosforado @5 10`
C03	`04`	`X`	`FRE`	`@0 Pesticide @5 11`
C03	`04`	`X`	`ENG`	`@0 Pesticides @5 11`
C03	`04`	`X`	`SPA`	`@0 Plaguicida @5 11`
C03	`05`	`X`	`FRE`	`@0 Huile olive @5 12`
C03	`05`	`X`	`ENG`	`@0 Olive oil @5 12`
C03	`05`	`X`	`SPA`	`@0 Aceite oliva @5 12`
C03	`06`	`X`	`FRE`	`@0 Biodétecteur @5 19`
C03	`06`	`X`	`ENG`	`@0 Biosensor @5 19`
C03	`06`	`X`	`SPA`	`@0 Biodetector @5 19`
C03	`07`	`X`	`FRE`	`@0 Anguille @5 20`
C03	`07`	`X`	`ENG`	`@0 Eel @5 20`
C03	`07`	`X`	`SPA`	`@0 Anguila @5 20`
C03	`08`	`X`	`FRE`	`@0 Insecticide @5 21`
C03	`08`	`X`	`ENG`	`@0 Insecticide @5 21`
C03	`08`	`X`	`SPA`	`@0 Insecticida @5 21`
C03	`09`	`X`	`FRE`	`@0 Ampérométrie @5 22`
C03	`09`	`X`	`ENG`	`@0 Amperometry @5 22`
C03	`09`	`X`	`SPA`	`@0 Amperometría @5 22`
C03	`10`	`X`	`FRE`	`@0 Electrode @5 24`
C03	`10`	`X`	`ENG`	`@0 Electrodes @5 24`
C03	`10`	`X`	`SPA`	`@0 Electrodo @5 24`
C07	`01`	`X`	`FRE`	`@0 Huile alimentaire @2 FX @5 08`
C07	`01`	`X`	`ENG`	`@0 Edible oil @2 FX @5 08`
C07	`01`	`X`	`SPA`	`@0 Aceite alimentario @2 FX @5 08`
C07	`02`	`X`	`FRE`	`@0 Huile végétale @5 49`
C07	`02`	`X`	`ENG`	`@0 Vegetable oil @5 49`
C07	`02`	`X`	`SPA`	`@0 Aceite vegetal @5 49`
C07	`03`	`X`	`FRE`	`@0 Poisson comestible @5 50`
C07	`03`	`X`	`ENG`	`@0 Edible fish @5 50`
C07	`03`	`X`	`SPA`	`@0 Pescado comestible @5 50`
N21				`@1 273`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 13-0288738 INIST
ET :	Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors
AU :	OUJJI (Najwa Ben); BAKAS (Idriss); ISTAMBOULIE (Georges); AIT-ICHOU (Ihya); AIT-ADDI (Elhabib); ROUILLON (Regis); NOGUER (Thierry)
AF :	University of Perpignan, IMAGES EA 4218, Building S 52 Av Paul Alduy/66860 Perpignan/France (1 aut., 2 aut., 3 aut., 6 aut., 7 aut.); University Ibn Zohr, AQUAMAR, Photocatalysis and Environment Team, Chemistry Department, Faculty of Science, BP 8106 Cité Dakhla/Agadir/Maroc (1 aut., 2 aut., 4 aut., 5 aut.)
DT :	Publication en série; Niveau analytique
SO :	Food control; ISSN 0956-7135; Royaume-Uni; Da. 2013; Vol. 30; No. 2; Pp. 657-661; Bibl. 1/4 p.
LA :	Anglais
EA :	This paper presents the construction of amperometric biosensors for the detection of organophosphorus insecticides widely used in the treatment of olive trees. The systems are based on the immobilisation of acetylcholinesterase on screen-printed electrodes by bioencapsulation in a sol-gel composite. The enzyme activity was estimated by measuring the thiocholine produced by the enzymatic hydrolysis of the acetylthiocholine chloride using cobalt phtalocyanine as mediator. The developed devices have been used to carry out inhibition studies with three pesticides: malathion, methidathion and dimethoate (in their oxidized form), and tested using standard solutions and real samples of olive oil. These biosensors showed good operational stability as they maintained their initial analytical signal response during 10 successive measurements, and a good reproducibility with a relative standard deviation of 3%. The limits of detection of the developed devices were very compatible with the maximum residue limit tolerated by international regulations, they were as low as 10^-9 M for the widely used pesticide malaoxon(oxidized malathion). The developed sensors were successfully used for the determinations of insecticides in real samples of olive oil.
CC :	002A35B08; 002A31C09B; 215
FD :	Procédé sol gel; Immobilisation; Organophosphoré; Pesticide; Huile olive; Biodétecteur; Anguille; Insecticide; Ampérométrie; Electrode
FG :	Huile alimentaire; Huile végétale; Poisson comestible
ED :	Sol gel process; Immobilization; Organophosphorus compounds; Pesticides; Olive oil; Biosensor; Eel; Insecticide; Amperometry; Electrodes
EG :	Edible oil; Vegetable oil; Edible fish
SD :	Procedimiento sol gel; Inmovilización; Organofosforado; Plaguicida; Aceite oliva; Biodetector; Anguila; Insecticida; Amperometría; Electrodo
LO :	INIST-26678.354000182547490480
ID :	13-0288738

Links to Exploration step

Pascal:13-0288738

Le document en format XML

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<front><div type="abstract" xml:lang="en">This paper presents the construction of amperometric biosensors for the detection of organophosphorus insecticides widely used in the treatment of olive trees. The systems are based on the immobilisation of acetylcholinesterase on screen-printed electrodes by bioencapsulation in a sol-gel composite. The enzyme activity was estimated by measuring the thiocholine produced by the enzymatic hydrolysis of the acetylthiocholine chloride using cobalt phtalocyanine as mediator. The developed devices have been used to carry out inhibition studies with three pesticides: malathion, methidathion and dimethoate (in their oxidized form), and tested using standard solutions and real samples of olive oil. These biosensors showed good operational stability as they maintained their initial analytical signal response during 10 successive measurements, and a good reproducibility with a relative standard deviation of 3%. The limits of detection of the developed devices were very compatible with the maximum residue limit tolerated by international regulations, they were as low as 10<sup>-9</sup>
 M for the widely used pesticide malaoxon(oxidized malathion). The developed sensors were successfully used for the determinations of insecticides in real samples of olive oil.</div>
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<s3>MAR</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
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<fC01 i1="01" l="ENG"><s0>This paper presents the construction of amperometric biosensors for the detection of organophosphorus insecticides widely used in the treatment of olive trees. The systems are based on the immobilisation of acetylcholinesterase on screen-printed electrodes by bioencapsulation in a sol-gel composite. The enzyme activity was estimated by measuring the thiocholine produced by the enzymatic hydrolysis of the acetylthiocholine chloride using cobalt phtalocyanine as mediator. The developed devices have been used to carry out inhibition studies with three pesticides: malathion, methidathion and dimethoate (in their oxidized form), and tested using standard solutions and real samples of olive oil. These biosensors showed good operational stability as they maintained their initial analytical signal response during 10 successive measurements, and a good reproducibility with a relative standard deviation of 3%. The limits of detection of the developed devices were very compatible with the maximum residue limit tolerated by international regulations, they were as low as 10<sup>-9</sup>
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<ET>Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors</ET>
<AU>OUJJI (Najwa Ben); BAKAS (Idriss); ISTAMBOULIE (Georges); AIT-ICHOU (Ihya); AIT-ADDI (Elhabib); ROUILLON (Regis); NOGUER (Thierry)</AU>
<AF>University of Perpignan, IMAGES EA 4218, Building S 52 Av Paul Alduy/66860 Perpignan/France (1 aut., 2 aut., 3 aut., 6 aut., 7 aut.); University Ibn Zohr, AQUAMAR, Photocatalysis and Environment Team, Chemistry Department, Faculty of Science, BP 8106 Cité Dakhla/Agadir/Maroc (1 aut., 2 aut., 4 aut., 5 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Food control; ISSN 0956-7135; Royaume-Uni; Da. 2013; Vol. 30; No. 2; Pp. 657-661; Bibl. 1/4 p.</SO>
<LA>Anglais</LA>
<EA>This paper presents the construction of amperometric biosensors for the detection of organophosphorus insecticides widely used in the treatment of olive trees. The systems are based on the immobilisation of acetylcholinesterase on screen-printed electrodes by bioencapsulation in a sol-gel composite. The enzyme activity was estimated by measuring the thiocholine produced by the enzymatic hydrolysis of the acetylthiocholine chloride using cobalt phtalocyanine as mediator. The developed devices have been used to carry out inhibition studies with three pesticides: malathion, methidathion and dimethoate (in their oxidized form), and tested using standard solutions and real samples of olive oil. These biosensors showed good operational stability as they maintained their initial analytical signal response during 10 successive measurements, and a good reproducibility with a relative standard deviation of 3%. The limits of detection of the developed devices were very compatible with the maximum residue limit tolerated by international regulations, they were as low as 10<sup>-9</sup>
 M for the widely used pesticide malaoxon(oxidized malathion). The developed sensors were successfully used for the determinations of insecticides in real samples of olive oil.</EA>
<CC>002A35B08; 002A31C09B; 215</CC>
<FD>Procédé sol gel; Immobilisation; Organophosphoré; Pesticide; Huile olive; Biodétecteur; Anguille; Insecticide; Ampérométrie; Electrode</FD>
<FG>Huile alimentaire; Huile végétale; Poisson comestible</FG>
<ED>Sol gel process; Immobilization; Organophosphorus compounds; Pesticides; Olive oil; Biosensor; Eel; Insecticide; Amperometry; Electrodes</ED>
<EG>Edible oil; Vegetable oil; Edible fish</EG>
<SD>Procedimiento sol gel; Inmovilización; Organofosforado; Plaguicida; Aceite oliva; Biodetector; Anguila; Insecticida; Amperometría; Electrodo</SD>
<LO>INIST-26678.354000182547490480</LO>
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Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors

Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors

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