Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes
Identifieur interne : 000476 ( Ncbi/Merge ); précédent : 000475; suivant : 000477Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes
Auteurs : Chioma C. Obi [Nigeria, États-Unis] ; Sunday A. Adebusoye [Nigeria] ; Esther O. Ugoji [Nigeria] ; Mathew O. Ilori [Nigeria] ; Olukayode O. Amund [Nigeria] ; William J. Hickey [États-Unis]Source :
- Frontiers in Microbiology [ 1664-302X ] ; 2016.
Abstract
Estuarine sediments are significant repositories of anthropogenic contaminants, and thus knowledge of the impacts of pollution upon microbial communities in these environments is important to understand potential effects on estuaries as a whole. The Lagos lagoon (Nigeria) is one of Africa’s largest estuarine ecosystems, and is impacted by hydrocarbon pollutants and other industrial and municipal wastes. The goal of this study was to elucidate microbial community structure in Lagos lagoon sediments to identify groups that may be adversely affected by pollution, and those that may serve as degraders of environmental contaminants, especially polycyclic aromatic hydrocarbons (PAHs). Sediment samples were collected from sites that ranged in types and levels of anthropogenic impacts. The sediments were characterized for a range of physicochemical properties, and microbial community structure was determined by Illumina sequencing of the 16S rRNA genes. Microbial diversity (species richness and evenness) in the Apapa and Eledu sediments was reduced compared to that of the Ofin site, and communities of both of the former two were dominated by a single operational taxonomic unit (OTU) assigned to the family
Url:
DOI: 10.3389/fmicb.2016.01213
PubMed: 27547200
PubMed Central: 4974257
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PMC:4974257Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>Estuarine sediments are significant repositories of anthropogenic contaminants, and thus knowledge of the impacts of pollution upon microbial communities in these environments is important to understand potential effects on estuaries as a whole. The Lagos lagoon (Nigeria) is one of Africa’s largest estuarine ecosystems, and is impacted by hydrocarbon pollutants and other industrial and municipal wastes. The goal of this study was to elucidate microbial community structure in Lagos lagoon sediments to identify groups that may be adversely affected by pollution, and those that may serve as degraders of environmental contaminants, especially polycyclic aromatic hydrocarbons (PAHs). Sediment samples were collected from sites that ranged in types and levels of anthropogenic impacts. The sediments were characterized for a range of physicochemical properties, and microbial community structure was determined by Illumina sequencing of the 16S rRNA genes. Microbial diversity (species richness and evenness) in the Apapa and Eledu sediments was reduced compared to that of the Ofin site, and communities of both of the former two were dominated by a single operational taxonomic unit (OTU) assigned to the family <italic>Helicobacteraceae</italic>
(Epsilonproteobacteria). In the Ofin community, Epsilonproteobacteria were minor constituents, while the major groups were Cyanobacteria, Bacteroidetes, and Firmicutes, which were all minor in the Apapa and Eledu sediments. Sediment oxygen demand (SOD), a broad indicator of contamination, was identified by multivariate analyses as strongly correlated with variation in alpha diversity. Environmental variables that explained beta diversity patterns included SOD, as well as levels of naphthalene, acenaphthylene, cobalt, cadmium, total organic matter, or nitrate. Of 582 OTU identified, abundance of 167 was significantly correlated (false discovery rate <italic>q</italic>
≤ 0.05) to environmental variables. The largest group of OTU correlated with PAH levels were PAH/hydrocarbon-degrading genera of the <italic>Oceanospirillales</italic>
order (Gammaproteobacteria), which were most abundant in the hydrocarbon-contaminated Apapa sediment. Similar <italic>Oceanospirillales</italic>
taxa are responsive to marine oil spills and thus may present a unifying theme in marine microbiology as bacteria adapted for degradation of high hydrocarbon loads, and may represent a potential means for intrinsic remediation in the case of the Lagos lagoon sediments.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Microbiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Front Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">Front. Microbiol.</journal-id>
<journal-title-group><journal-title>Frontiers in Microbiology</journal-title>
</journal-title-group>
<issn pub-type="epub">1664-302X</issn>
<publisher><publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">27547200</article-id>
<article-id pub-id-type="pmc">4974257</article-id>
<article-id pub-id-type="doi">10.3389/fmicb.2016.01213</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Microbiology</subject>
<subj-group><subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Obi</surname>
<given-names>Chioma C.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="author-notes" rid="fn001"><sup>*</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/335652/overview"></uri>
</contrib>
<contrib contrib-type="author"><name><surname>Adebusoye</surname>
<given-names>Sunday A.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ugoji</surname>
<given-names>Esther O.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ilori</surname>
<given-names>Mathew O.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Amund</surname>
<given-names>Olukayode O.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hickey</surname>
<given-names>William J.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/22359/overview"></uri>
</contrib>
</contrib-group>
<aff id="aff1"><sup>1</sup>
<institution>Department of Microbiology, University of Lagos</institution>
<country>Lagos, Nigeria</country>
</aff>
<aff id="aff2"><sup>2</sup>
<institution>O.N. Allen Laboratory for Soil Microbiology, Department of Soil Science, University of Wisconsin–Madison, Madison</institution>
<country>WI, USA</country>
</aff>
<author-notes><fn fn-type="edited-by"><p>Edited by: <italic>Wael Ahmed Ismail, Arabian Gulf University, Bahrain</italic>
</p>
</fn>
<fn fn-type="edited-by"><p>Reviewed by: <italic>Bongkeun Song, Virginia Institute of Marine Science, USA; Gian Marco Luna, National Research Council, Italy; Ana E. Escalante, National Autonomous University of Mexico, Mexico</italic>
</p>
</fn>
<corresp id="fn001">*Correspondence: <italic>Chioma C. Obi, <email xlink:type="simple">chioma.obi@students.unilag.edu.ng</email>
</italic>
</corresp>
<fn fn-type="other" id="fn002"><p>This article was submitted to Microbiotechnology, Ecotoxicology and Bioremediation, a section of the journal Frontiers in Microbiology</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>05</day>
<month>8</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="collection"><year>2016</year>
</pub-date>
<volume>7</volume>
<elocation-id>1213</elocation-id>
<history><date date-type="received"><day>14</day>
<month>3</month>
<year>2016</year>
</date>
<date date-type="accepted"><day>21</day>
<month>7</month>
<year>2016</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2016 Obi, Adebusoye, Ugoji, Ilori, Amund and Hickey.</copyright-statement>
<copyright-year>2016</copyright-year>
<copyright-holder>Obi, Adebusoye, Ugoji, Ilori, Amund and Hickey</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p>
</license>
</permissions>
<abstract><p>Estuarine sediments are significant repositories of anthropogenic contaminants, and thus knowledge of the impacts of pollution upon microbial communities in these environments is important to understand potential effects on estuaries as a whole. The Lagos lagoon (Nigeria) is one of Africa’s largest estuarine ecosystems, and is impacted by hydrocarbon pollutants and other industrial and municipal wastes. The goal of this study was to elucidate microbial community structure in Lagos lagoon sediments to identify groups that may be adversely affected by pollution, and those that may serve as degraders of environmental contaminants, especially polycyclic aromatic hydrocarbons (PAHs). Sediment samples were collected from sites that ranged in types and levels of anthropogenic impacts. The sediments were characterized for a range of physicochemical properties, and microbial community structure was determined by Illumina sequencing of the 16S rRNA genes. Microbial diversity (species richness and evenness) in the Apapa and Eledu sediments was reduced compared to that of the Ofin site, and communities of both of the former two were dominated by a single operational taxonomic unit (OTU) assigned to the family <italic>Helicobacteraceae</italic>
(Epsilonproteobacteria). In the Ofin community, Epsilonproteobacteria were minor constituents, while the major groups were Cyanobacteria, Bacteroidetes, and Firmicutes, which were all minor in the Apapa and Eledu sediments. Sediment oxygen demand (SOD), a broad indicator of contamination, was identified by multivariate analyses as strongly correlated with variation in alpha diversity. Environmental variables that explained beta diversity patterns included SOD, as well as levels of naphthalene, acenaphthylene, cobalt, cadmium, total organic matter, or nitrate. Of 582 OTU identified, abundance of 167 was significantly correlated (false discovery rate <italic>q</italic>
≤ 0.05) to environmental variables. The largest group of OTU correlated with PAH levels were PAH/hydrocarbon-degrading genera of the <italic>Oceanospirillales</italic>
order (Gammaproteobacteria), which were most abundant in the hydrocarbon-contaminated Apapa sediment. Similar <italic>Oceanospirillales</italic>
taxa are responsive to marine oil spills and thus may present a unifying theme in marine microbiology as bacteria adapted for degradation of high hydrocarbon loads, and may represent a potential means for intrinsic remediation in the case of the Lagos lagoon sediments.</p>
</abstract>
<kwd-group><kwd>sediment</kwd>
<kwd>PAH</kwd>
<kwd>hydrocarbons</kwd>
<kwd>microbial diversity</kwd>
<kwd>Illumina</kwd>
<kwd>Africa</kwd>
<kwd>estuary</kwd>
<kwd>Lagos lagoon</kwd>
</kwd-group>
<counts><fig-count count="9"></fig-count>
<table-count count="2"></table-count>
<equation-count count="0"></equation-count>
<ref-count count="77"></ref-count>
<page-count count="16"></page-count>
<word-count count="0"></word-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Nigeria</li>
<li>États-Unis</li>
</country>
</list>
<tree><country name="Nigeria"><noRegion><name sortKey="Obi, Chioma C" sort="Obi, Chioma C" uniqKey="Obi C" first="Chioma C." last="Obi">Chioma C. Obi</name>
</noRegion>
<name sortKey="Adebusoye, Sunday A" sort="Adebusoye, Sunday A" uniqKey="Adebusoye S" first="Sunday A." last="Adebusoye">Sunday A. Adebusoye</name>
<name sortKey="Amund, Olukayode O" sort="Amund, Olukayode O" uniqKey="Amund O" first="Olukayode O." last="Amund">Olukayode O. Amund</name>
<name sortKey="Ilori, Mathew O" sort="Ilori, Mathew O" uniqKey="Ilori M" first="Mathew O." last="Ilori">Mathew O. Ilori</name>
<name sortKey="Ugoji, Esther O" sort="Ugoji, Esther O" uniqKey="Ugoji E" first="Esther O." last="Ugoji">Esther O. Ugoji</name>
</country>
<country name="États-Unis"><noRegion><name sortKey="Obi, Chioma C" sort="Obi, Chioma C" uniqKey="Obi C" first="Chioma C." last="Obi">Chioma C. Obi</name>
</noRegion>
<name sortKey="Hickey, William J" sort="Hickey, William J" uniqKey="Hickey W" first="William J." last="Hickey">William J. Hickey</name>
</country>
</tree>
</affiliations>
</record>
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