Recombinant Pvs48/45 Antigen Expressed in E. coli Generates Antibodies that Block Malaria Transmission in Anopheles albimanus Mosquitoes
Identifieur interne : 000373 ( Ncbi/Merge ); précédent : 000372; suivant : 000374Recombinant Pvs48/45 Antigen Expressed in E. coli Generates Antibodies that Block Malaria Transmission in Anopheles albimanus Mosquitoes
Auteurs : Myriam Arévalo-Herrera [Colombie] ; Andrés F. Vallejo [Colombie] ; Kelly Rubiano [Colombie] ; Yezid Solarte [Colombie] ; Catherin Marin [Colombie] ; Angélica Castellanos [Colombie] ; Nora Céspedes [Colombie] ; S Crates Herrera [Colombie]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
Abstract
Transmission of malaria parasites from humans to
Url:
DOI: 10.1371/journal.pone.0119335
PubMed: 25775466
PubMed Central: 4361554
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Recombinant <italic>Pvs</italic>48/45 Antigen Expressed in <italic>E</italic>. <italic>coli</italic> Generates Antibodies that Block Malaria Transmission in <italic>Anopheles albimanus</italic> Mosquitoes</title><author><name sortKey="Arevalo Herrera, Myriam" sort="Arevalo Herrera, Myriam" uniqKey="Arevalo Herrera M" first="Myriam" last="Arévalo-Herrera">Myriam Arévalo-Herrera</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Malaria Vaccine and Drug Development Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Malaria Vaccine and Drug Development Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>School of Health, Universidad del Valle, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>School of Health, Universidad del Valle, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Vallejo, Andres F" sort="Vallejo, Andres F" uniqKey="Vallejo A" first="Andrés F." last="Vallejo">Andrés F. Vallejo</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Caucaseco Scientific Research Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Caucaseco Scientific Research Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Rubiano, Kelly" sort="Rubiano, Kelly" uniqKey="Rubiano K" first="Kelly" last="Rubiano">Kelly Rubiano</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Malaria Vaccine and Drug Development Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Malaria Vaccine and Drug Development Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Caucaseco Scientific Research Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Caucaseco Scientific 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sortKey="Castellanos, Angelica" sort="Castellanos, Angelica" uniqKey="Castellanos A" first="Angélica" last="Castellanos">Angélica Castellanos</name><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Primates Center Foundation, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Primates Center Foundation, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Cespedes, Nora" sort="Cespedes, Nora" uniqKey="Cespedes N" first="Nora" last="Céspedes">Nora Céspedes</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Malaria Vaccine and Drug Development Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Malaria Vaccine and Drug Development Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Herrera, S Crates" sort="Herrera, S Crates" uniqKey="Herrera S" first="S 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<italic>Pvs</italic>48/45 Antigen Expressed in <italic>E</italic>. <italic>coli</italic> Generates Antibodies that Block Malaria Transmission in <italic>Anopheles albimanus</italic> Mosquitoes</title><author><name sortKey="Arevalo Herrera, Myriam" sort="Arevalo Herrera, Myriam" uniqKey="Arevalo Herrera M" first="Myriam" last="Arévalo-Herrera">Myriam Arévalo-Herrera</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Malaria Vaccine and Drug Development Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Malaria Vaccine and Drug Development Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>School of Health, Universidad del Valle, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>School of Health, Universidad del Valle, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Vallejo, Andres F" sort="Vallejo, Andres F" uniqKey="Vallejo A" first="Andrés F." last="Vallejo">Andrés F. Vallejo</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Caucaseco Scientific Research Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Caucaseco Scientific Research Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Rubiano, Kelly" sort="Rubiano, Kelly" uniqKey="Rubiano K" first="Kelly" last="Rubiano">Kelly Rubiano</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Malaria Vaccine and Drug Development Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Malaria Vaccine and Drug Development Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Caucaseco Scientific Research Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Caucaseco Scientific Research Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Solarte, Yezid" sort="Solarte, Yezid" uniqKey="Solarte Y" first="Yezid" last="Solarte">Yezid Solarte</name><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>School of Health, Universidad del Valle, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>School of Health, Universidad del Valle, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Marin, Catherin" sort="Marin, Catherin" uniqKey="Marin C" first="Catherin" last="Marin">Catherin Marin</name><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Primates Center Foundation, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Primates Center Foundation, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Castellanos, Angelica" sort="Castellanos, Angelica" uniqKey="Castellanos A" first="Angélica" last="Castellanos">Angélica Castellanos</name><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Primates Center Foundation, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Primates Center Foundation, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Cespedes, Nora" sort="Cespedes, Nora" uniqKey="Cespedes N" first="Nora" last="Céspedes">Nora Céspedes</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Malaria Vaccine and Drug Development Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Malaria Vaccine and Drug Development Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author><author><name sortKey="Herrera, S Crates" sort="Herrera, S Crates" uniqKey="Herrera S" first="S Crates" last="Herrera">S Crates Herrera</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Malaria Vaccine and Drug Development Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Malaria Vaccine and Drug Development Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Caucaseco Scientific Research Center, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Caucaseco Scientific Research Center, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Primates Center Foundation, Cali, Colombia</addr-line></nlm:aff><country xml:lang="fr">Colombie</country><wicri:regionArea>Primates Center Foundation, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Transmission of malaria parasites from humans to <italic>Anopheles</italic> mosquitoes can be inhibited by specific antibodies elicited during malaria infection, which target surface <italic>Plasmodium</italic> gametocyte/gamete proteins. Some of these proteins may have potential for vaccine development. <italic>Pvs</italic>48/45 is a <italic>P</italic>. <italic>vivax</italic> gametocyte surface antigen orthologous to <italic>Pfs</italic>48/45, which may play a role during parasite fertilization and thus has potential for transmission blocking (TB) activity. Here we describe the expression of a recombinant <italic>Pvs</italic>48/45 protein expressed in <italic>Escherichia coli</italic> as a ∼60kDa construct which we tested for antigenicity using human sera and for its immunogenicity and transmission blocking activity of specific anti-mouse and anti-monkey <italic>Pvs</italic>48/45 antibodies. The protein reacted with sera of individuals from malaria-endemic areas and in addition induced specific IgG antibody responses in BALB/c mice and <italic>Aotus l</italic>. <italic>griseimembra</italic> monkeys. Sera from both immunized animal species recognized native <italic>P</italic>. <italic>vivax</italic> protein in Western blot (WB) and immunofluorescence assays. Moreover, sera from immunized mice and monkeys produced significant inhibition of parasite transmission to <italic>An</italic>. <italic>Albimanus</italic> mosquitoes as shown by membrane feeding assays. Results indicate the presence of reactive epitopes in the <italic>Pvs</italic>48/45 recombinant product that induce antibodies with TB activity. Further testing of this protein is ongoing to determine its vaccine potential.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Baird, J" uniqKey="Baird J">J Baird</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Snow, Rw" uniqKey="Snow R">RW Snow</name></author><author><name sortKey="Omumbo, Ja" uniqKey="Omumbo J">JA Omumbo</name></author><author><name sortKey="Lowe, B" uniqKey="Lowe B">B Lowe</name></author><author><name sortKey="Molyneux, Cs" uniqKey="Molyneux C">CS Molyneux</name></author><author><name sortKey="Obiero, J O" uniqKey="Obiero J">J-O Obiero</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doolan, Dl" uniqKey="Doolan D">DL Doolan</name></author><author><name sortKey="Dobano, C" uniqKey="Dobano C">C Dobano</name></author><author><name sortKey="Baird, Jk" uniqKey="Baird J">JK Baird</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mendis, C" uniqKey="Mendis C">C Mendis</name></author><author><name sortKey="Munesinghe, Yd" uniqKey="Munesinghe Y">YD Munesinghe</name></author><author><name sortKey="De Silva, Yny" uniqKey="De Silva Y">YNY de Silva</name></author><author><name sortKey="Keragalla, I" uniqKey="Keragalla I">I Keragalla</name></author><author><name sortKey="Carter, R" uniqKey="Carter R">R Carter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grotendorst, Ca" uniqKey="Grotendorst C">CA Grotendorst</name></author><author><name sortKey="Kumar, N" uniqKey="Kumar N">N Kumar</name></author><author><name sortKey="Carter, R" uniqKey="Carter R">R Carter</name></author><author><name sortKey="Kaushal, Dc" uniqKey="Kaushal D">DC Kaushal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaushal, Dc" uniqKey="Kaushal D">DC Kaushal</name></author><author><name sortKey="Carter, R" uniqKey="Carter R">R Carter</name></author><author><name sortKey="Rener, J" uniqKey="Rener J">J Rener</name></author><author><name sortKey="Grotendorst, Ca" uniqKey="Grotendorst C">CA Grotendorst</name></author><author><name sortKey="Miller, Lh" uniqKey="Miller L">LH Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hisaeda, H" uniqKey="Hisaeda H">H Hisaeda</name></author><author><name sortKey="Stowers, Aw" uniqKey="Stowers A">AW Stowers</name></author><author><name sortKey="Tsuboi, T" uniqKey="Tsuboi T">T Tsuboi</name></author><author><name sortKey="Collins, We" uniqKey="Collins W">WE Collins</name></author><author><name sortKey="Sattabongkot, Js" uniqKey="Sattabongkot J">JS Sattabongkot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kubler Kielb, J" uniqKey="Kubler Kielb J">J Kubler-Kielb</name></author><author><name sortKey="Majadly, F" uniqKey="Majadly F">F Majadly</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name></author><author><name sortKey="Narum, Dl" uniqKey="Narum D">DL Narum</name></author><author><name sortKey="Guo, C" uniqKey="Guo C">C Guo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Collins, We" uniqKey="Collins W">WE Collins</name></author><author><name sortKey="Barnwell, Jw" uniqKey="Barnwell J">JW Barnwell</name></author><author><name sortKey="Sullivan, Js" uniqKey="Sullivan J">JS Sullivan</name></author><author><name sortKey="Nace, D" uniqKey="Nace D">D Nace</name></author><author><name sortKey="Williams, T" uniqKey="Williams T">T Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arevalo Herrera, M" uniqKey="Arevalo Herrera M">M Arevalo-Herrera</name></author><author><name sortKey="Solarte, Y" uniqKey="Solarte Y">Y Solarte</name></author><author><name sortKey="Yasnot, Mf" uniqKey="Yasnot M">MF Yasnot</name></author><author><name sortKey="Castellanos, A" uniqKey="Castellanos A">A Castellanos</name></author><author><name sortKey="Rincon, A" uniqKey="Rincon A">A Rincon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arevalo Herrera, M" uniqKey="Arevalo Herrera M">M Arevalo-Herrera</name></author><author><name sortKey="Solarte, Y" uniqKey="Solarte Y">Y Solarte</name></author><author><name sortKey="Zamora, F" uniqKey="Zamora F">F Zamora</name></author><author><name sortKey="Mendez, F" uniqKey="Mendez F">F Mendez</name></author><author><name sortKey="Yasnot, Mf" uniqKey="Yasnot M">MF Yasnot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bousema, T" uniqKey="Bousema T">T Bousema</name></author><author><name sortKey="Roeffen, W" uniqKey="Roeffen W">W Roeffen</name></author><author><name sortKey="Meijerink, H" uniqKey="Meijerink H">H Meijerink</name></author><author><name sortKey="Mwerinde, H" uniqKey="Mwerinde H">H Mwerinde</name></author><author><name sortKey="Mwakalinga, S" uniqKey="Mwakalinga S">S Mwakalinga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gamage Mendis, Ac" uniqKey="Gamage Mendis A">AC Gamage-Mendis</name></author><author><name sortKey="Rajakaruna, J" uniqKey="Rajakaruna J">J Rajakaruna</name></author><author><name sortKey="Carter, R" uniqKey="Carter R">R Carter</name></author><author><name sortKey="Mendis, Kn" uniqKey="Mendis K">KN Mendis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tachibana, M" uniqKey="Tachibana M">M Tachibana</name></author><author><name sortKey="Sato, C" uniqKey="Sato C">C Sato</name></author><author><name sortKey="Otsuki, H" uniqKey="Otsuki H">H Otsuki</name></author><author><name sortKey="Sattabongkot, J" uniqKey="Sattabongkot J">J Sattabongkot</name></author><author><name sortKey="Kaneko, O" uniqKey="Kaneko O">O Kaneko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Outchkourov, Ns" uniqKey="Outchkourov N">NS Outchkourov</name></author><author><name sortKey="Roeffen, W" uniqKey="Roeffen W">W Roeffen</name></author><author><name sortKey="Kaan, A" uniqKey="Kaan A">A Kaan</name></author><author><name sortKey="Jansen, J" uniqKey="Jansen J">J Jansen</name></author><author><name sortKey="Luty, A" uniqKey="Luty A">A Luty</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chowdhury, Dr" uniqKey="Chowdhury D">DR Chowdhury</name></author><author><name sortKey="Angov, E" uniqKey="Angov E">E Angov</name></author><author><name sortKey="Kariuki, T" uniqKey="Kariuki T">T Kariuki</name></author><author><name sortKey="Kumar, N" uniqKey="Kumar N">N Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barr, Pj" uniqKey="Barr P">PJ Barr</name></author><author><name sortKey="Green, Km" uniqKey="Green K">KM Green</name></author><author><name sortKey="Gibson, Hl" uniqKey="Gibson H">HL Gibson</name></author><author><name sortKey="Bathurst, Ic" uniqKey="Bathurst I">IC Bathurst</name></author><author><name sortKey="Quakyi, Ia" uniqKey="Quakyi I">IA Quakyi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaslow, Dc" uniqKey="Kaslow D">DC Kaslow</name></author><author><name sortKey="Bathurst, Ic" uniqKey="Bathurst I">IC Bathurst</name></author><author><name sortKey="Lensen, T" uniqKey="Lensen T">T Lensen</name></author><author><name sortKey="Ponnudurai, T" uniqKey="Ponnudurai T">T Ponnudurai</name></author><author><name sortKey="Barr, Pj" uniqKey="Barr P">PJ Barr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carter, R" uniqKey="Carter R">R Carter</name></author><author><name sortKey="Graves, P" uniqKey="Graves P">P Graves</name></author><author><name sortKey="Keister, D" uniqKey="Keister D">D Keister</name></author><author><name sortKey="Quakyi, I" uniqKey="Quakyi I">I Quakyi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mulder, B" uniqKey="Mulder B">B Mulder</name></author><author><name sortKey="Lensen, T" uniqKey="Lensen T">T Lensen</name></author><author><name sortKey="Tchuinkam, T" uniqKey="Tchuinkam T">T Tchuinkam</name></author><author><name sortKey="Roeffen, W" uniqKey="Roeffen W">W Roeffen</name></author><author><name sortKey="Verhave, Jp" uniqKey="Verhave J">JP Verhave</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roeffen, W" uniqKey="Roeffen W">W Roeffen</name></author><author><name sortKey="Mulder, B" uniqKey="Mulder B">B Mulder</name></author><author><name sortKey="Teelen, K" uniqKey="Teelen K">K Teelen</name></author><author><name sortKey="Bolmer, M" uniqKey="Bolmer M">M Bolmer</name></author><author><name sortKey="Eling, W" uniqKey="Eling W">W Eling</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Dijk, Mr" uniqKey="Van Dijk M">MR van Dijk</name></author><author><name sortKey="Janse, Cj" uniqKey="Janse C">CJ Janse</name></author><author><name sortKey="Thompson, J" uniqKey="Thompson J">J Thompson</name></author><author><name sortKey="Waters, Ap" uniqKey="Waters A">AP Waters</name></author><author><name sortKey="Braks, Ja" uniqKey="Braks J">JA Braks</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pradel, G" uniqKey="Pradel G">G Pradel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arevalo Herrera, M" uniqKey="Arevalo Herrera M">M Arevalo-Herrera</name></author><author><name sortKey="Solarte, Y" uniqKey="Solarte Y">Y Solarte</name></author><author><name sortKey="Yasnot, Mf" uniqKey="Yasnot M">MF Yasnot</name></author><author><name sortKey="Castellanos, A" uniqKey="Castellanos A">A Castellanos</name></author><author><name sortKey="Rincon, A" uniqKey="Rincon A">A Rincon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blagborough, Am" uniqKey="Blagborough A">AM Blagborough</name></author><author><name sortKey="Yoshida, S" uniqKey="Yoshida S">S Yoshida</name></author><author><name sortKey="Sattabongkot, J" uniqKey="Sattabongkot J">J Sattabongkot</name></author><author><name sortKey="Tsuboi, T" uniqKey="Tsuboi T">T Tsuboi</name></author><author><name sortKey="Sinden, Re" uniqKey="Sinden R">RE Sinden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaslow, Dc" uniqKey="Kaslow D">DC Kaslow</name></author><author><name sortKey="Shiloach, J" uniqKey="Shiloach J">J Shiloach</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name></author><author><name sortKey="Ellis, Rd" uniqKey="Ellis R">RD Ellis</name></author><author><name sortKey="Shaffer, D" uniqKey="Shaffer D">D Shaffer</name></author><author><name sortKey="Fontes, E" uniqKey="Fontes E">E Fontes</name></author><author><name sortKey="Malkin, Em" uniqKey="Malkin E">EM Malkin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Malkin, Em" uniqKey="Malkin E">EM Malkin</name></author><author><name sortKey="Durbin, Ap" uniqKey="Durbin A">AP Durbin</name></author><author><name sortKey="Diemert, Dj" uniqKey="Diemert D">DJ Diemert</name></author><author><name sortKey="Sattabongkot, J" uniqKey="Sattabongkot J">J Sattabongkot</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carter, R" uniqKey="Carter R">R Carter</name></author><author><name sortKey="Coulson, A" uniqKey="Coulson A">A Coulson</name></author><author><name sortKey="Bhatti, S" uniqKey="Bhatti S">S Bhatti</name></author><author><name sortKey="Taylor, Bj" uniqKey="Taylor B">BJ Taylor</name></author><author><name sortKey="Elliott, Jf" uniqKey="Elliott J">JF Elliott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baneyx, F" uniqKey="Baneyx F">F Baneyx</name></author><author><name sortKey="Mujacic, M" uniqKey="Mujacic M">M Mujacic</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alcala, P" uniqKey="Alcala P">P Alcalá</name></author><author><name sortKey="Ferrer Miralles, N" uniqKey="Ferrer Miralles N">N Ferrer-Miralles</name></author><author><name sortKey="Villaverde, A" uniqKey="Villaverde A">A Villaverde</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Collins, We" uniqKey="Collins W">WE Collins</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Gaspar, P" uniqKey="Gaspar P">P Gaspar</name></author><author><name sortKey="Oliveira, Jl" uniqKey="Oliveira J">JL Oliveira</name></author><author><name sortKey="Frommlet, J" uniqKey="Frommlet J">J Frommlet</name></author><author><name sortKey="Santos, Ma" uniqKey="Santos M">MA Santos</name></author><author><name sortKey="Moura, G" uniqKey="Moura G">G Moura</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Shevchenko, A" uniqKey="Shevchenko A">A Shevchenko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blake, M" uniqKey="Blake M">M Blake</name></author><author><name sortKey="Johnston, K" uniqKey="Johnston K">K Johnston</name></author><author><name sortKey="Russell Jones, G" uniqKey="Russell Jones G">G Russell-Jones</name></author><author><name sortKey="Gotschlich, E" uniqKey="Gotschlich E">E Gotschlich</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Herrera, S" uniqKey="Herrera S">S Herrera</name></author><author><name sortKey="Gomez, A" uniqKey="Gomez A">A Gomez</name></author><author><name sortKey="Vera, O" uniqKey="Vera O">O Vera</name></author><author><name sortKey="Vergara, J" uniqKey="Vergara J">J Vergara</name></author><author><name sortKey="Valderrama Aguirre, A" uniqKey="Valderrama Aguirre A">A Valderrama-Aguirre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Terpe, K" uniqKey="Terpe K">K Terpe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doug, Chung D W" uniqKey="Doug C">Chung D-W Doug</name></author><author><name sortKey="Ponts, N" uniqKey="Ponts N">N Ponts</name></author><author><name sortKey="Cervantes, S" uniqKey="Cervantes S">S Cervantes</name></author><author><name sortKey="Le Roch, Kg" uniqKey="Le Roch K">KG Le Roch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carlton, Jm" uniqKey="Carlton J">JM Carlton</name></author><author><name sortKey="Adams, Jh" uniqKey="Adams J">JH Adams</name></author><author><name sortKey="Silva, Jc" uniqKey="Silva J">JC Silva</name></author><author><name sortKey="Bidwell, Sl" uniqKey="Bidwell S">SL Bidwell</name></author><author><name sortKey="Lorenzi, H" uniqKey="Lorenzi H">H Lorenzi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pain, A" uniqKey="Pain A">A Pain</name></author><author><name sortKey="Hertz Fowler, C" uniqKey="Hertz Fowler C">C Hertz-Fowler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sahdev, S" uniqKey="Sahdev S">S Sahdev</name></author><author><name sortKey="Khattar, Sk" uniqKey="Khattar S">SK Khattar</name></author><author><name sortKey="Saini, Ks" uniqKey="Saini K">KS Saini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Birkholtz, Lm" uniqKey="Birkholtz L">LM Birkholtz</name></author><author><name sortKey="Blatch, G" uniqKey="Blatch G">G Blatch</name></author><author><name sortKey="Coetzer, Tl" uniqKey="Coetzer T">TL Coetzer</name></author><author><name sortKey="Hoppe, Hc" uniqKey="Hoppe H">HC Hoppe</name></author><author><name sortKey="Human, E" uniqKey="Human E">E Human</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25775466</article-id><article-id pub-id-type="pmc">4361554</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0119335</article-id><article-id pub-id-type="publisher-id">PONE-D-14-29883</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Recombinant <italic>Pvs</italic>48/45 Antigen Expressed in <italic>E</italic>. <italic>coli</italic> Generates Antibodies that Block Malaria Transmission in <italic>Anopheles albimanus</italic> Mosquitoes</article-title><alt-title alt-title-type="running-head">Anti-<italic>Pvs</italic>48/45 Antibodies Block Malaria Transmission</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Arévalo-Herrera</surname><given-names>Myriam</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref><xref rid="cor001" ref-type="corresp">*</xref></contrib><contrib contrib-type="author"><name><surname>Vallejo</surname><given-names>Andrés F.</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Rubiano</surname><given-names>Kelly</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Solarte</surname><given-names>Yezid</given-names></name><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Marin</surname><given-names>Catherin</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Castellanos</surname><given-names>Angélica</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Céspedes</surname><given-names>Nora</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Herrera</surname><given-names>Sócrates</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Malaria Vaccine and Drug Development Center, Cali, Colombia</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Caucaseco Scientific Research Center, Cali, Colombia</addr-line></aff><aff id="aff003"><label>3</label><addr-line>School of Health, Universidad del Valle, Cali, Colombia</addr-line></aff><aff id="aff004"><label>4</label><addr-line>Primates Center Foundation, Cali, Colombia</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Tsuboi</surname><given-names>Takafumi</given-names></name><role>Academic Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Ehime University, JAPAN</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: MAH SH. Performed the experiments: AFV KR YS CM AC NC. Analyzed the data: AFV KR NC. Contributed reagents/materials/analysis tools: MAH. Wrote the paper: MAH AFV KR NC SH.</p></fn><corresp id="cor001">* E-mail: <email>marevalo@inmuno.org</email></corresp></author-notes><pub-date pub-type="epub"><day>16</day><month>3</month><year>2015</year></pub-date><pub-date pub-type="collection"><year>2015</year></pub-date><volume>10</volume><issue>3</issue><elocation-id>e0119335</elocation-id><history><date date-type="received"><day>2</day><month>8</month><year>2014</year></date><date date-type="accepted"><day>23</day><month>1</month><year>2015</year></date></history><permissions><copyright-year>2015</copyright-year><copyright-holder>Arévalo-Herrera et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="pone.0119335.pdf"></self-uri><abstract><p>Transmission of malaria parasites from humans to <italic>Anopheles</italic> mosquitoes can be inhibited by specific antibodies elicited during malaria infection, which target surface <italic>Plasmodium</italic> gametocyte/gamete proteins. Some of these proteins may have potential for vaccine development. <italic>Pvs</italic>48/45 is a <italic>P</italic>. <italic>vivax</italic> gametocyte surface antigen orthologous to <italic>Pfs</italic>48/45, which may play a role during parasite fertilization and thus has potential for transmission blocking (TB) activity. Here we describe the expression of a recombinant <italic>Pvs</italic>48/45 protein expressed in <italic>Escherichia coli</italic> as a ∼60kDa construct which we tested for antigenicity using human sera and for its immunogenicity and transmission blocking activity of specific anti-mouse and anti-monkey <italic>Pvs</italic>48/45 antibodies. The protein reacted with sera of individuals from malaria-endemic areas and in addition induced specific IgG antibody responses in BALB/c mice and <italic>Aotus l</italic>. <italic>griseimembra</italic> monkeys. Sera from both immunized animal species recognized native <italic>P</italic>. <italic>vivax</italic> protein in Western blot (WB) and immunofluorescence assays. Moreover, sera from immunized mice and monkeys produced significant inhibition of parasite transmission to <italic>An</italic>. <italic>Albimanus</italic> mosquitoes as shown by membrane feeding assays. Results indicate the presence of reactive epitopes in the <italic>Pvs</italic>48/45 recombinant product that induce antibodies with TB activity. Further testing of this protein is ongoing to determine its vaccine potential.</p></abstract><funding-group><funding-statement>This work was funded by Colciencias (Contracts 360-2011, 685-2013 and 719-2013) and by the National Institutes of Health (NIH) (Grant number 5U19AI089702). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><fig-count count="5"></fig-count><table-count count="3"></table-count><page-count count="16"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper.</p></notes></front></pmc><affiliations><list><country><li>Colombie</li></country></list><tree><country name="Colombie"><noRegion><name sortKey="Arevalo Herrera, Myriam" sort="Arevalo Herrera, Myriam" uniqKey="Arevalo Herrera M" first="Myriam" last="Arévalo-Herrera">Myriam Arévalo-Herrera</name></noRegion><name sortKey="Arevalo Herrera, Myriam" sort="Arevalo Herrera, Myriam" uniqKey="Arevalo Herrera M" first="Myriam" last="Arévalo-Herrera">Myriam Arévalo-Herrera</name><name sortKey="Castellanos, Angelica" sort="Castellanos, Angelica" uniqKey="Castellanos A" first="Angélica" last="Castellanos">Angélica Castellanos</name><name sortKey="Cespedes, Nora" sort="Cespedes, Nora" uniqKey="Cespedes N" first="Nora" last="Céspedes">Nora Céspedes</name><name sortKey="Herrera, S Crates" sort="Herrera, S Crates" uniqKey="Herrera S" first="S Crates" last="Herrera">S Crates Herrera</name><name sortKey="Herrera, S Crates" sort="Herrera, S Crates" uniqKey="Herrera S" first="S Crates" last="Herrera">S Crates Herrera</name><name sortKey="Herrera, S Crates" sort="Herrera, S Crates" uniqKey="Herrera S" first="S Crates" last="Herrera">S Crates Herrera</name><name sortKey="Marin, Catherin" sort="Marin, Catherin" uniqKey="Marin C" first="Catherin" last="Marin">Catherin Marin</name><name sortKey="Rubiano, Kelly" sort="Rubiano, Kelly" uniqKey="Rubiano K" first="Kelly" last="Rubiano">Kelly Rubiano</name><name sortKey="Rubiano, Kelly" sort="Rubiano, Kelly" uniqKey="Rubiano K" first="Kelly" last="Rubiano">Kelly Rubiano</name><name sortKey="Solarte, Yezid" sort="Solarte, Yezid" uniqKey="Solarte Y" first="Yezid" last="Solarte">Yezid Solarte</name><name sortKey="Vallejo, Andres F" sort="Vallejo, Andres F" uniqKey="Vallejo A" first="Andrés F." last="Vallejo">Andrés F. 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