Neurotoxic glutamate treatment of cultured cerebellar granule cells induces Ca2+-dependent collapse of mitochondrial membrane potential and ultrastructural alterations of mitochondria
Identifieur interne : 000F91 ( Main/Exploration ); précédent : 000F90; suivant : 000F92Neurotoxic glutamate treatment of cultured cerebellar granule cells induces Ca2+-dependent collapse of mitochondrial membrane potential and ultrastructural alterations of mitochondria
Auteurs : Nikolaj K. Isaev [Russie] ; Dmitry B. Zorov [Russie] ; Elena V. Stelmashook [Russie] ; Rustem E. Uzbekov [Russie] ; Maxim B. Kozhemyakin [Russie] ; Ilya V. Victorov [Russie]Source :
- FEBS Letters [ 0014-5793 ] ; 1996.
Abstract
Rhodamine 123 staining and electron microscopy were used to reveal a correlation between the ultrastructural and functional state of cultured cerebellar granule cells after short glutamate treatment. Glutamate exposure (15 min, 100 μM) in Mg2+-free solution caused considerable ultrastructural alterations in a granule cell: clumping of the chromatin, swelling of the endoplasmic reticulum and mitochondria, and disruption of the mitochondrial cristae. After glutamate treatment, the mitochondria of the neurons lost their ability to sequester rhodamine 123. Both the N-ethyl-d-aspartate receptor channel blocker MK-801 (30 μM) and cobalt chloride (2 mM) prevented the deteriorative effects of glutamate. These data suggest that glutamate-induced Ca2+ overload of the neurons can lead to non-specific permeability of the inner mitochondrial membrane, resulting in neuronal death.
Url:
DOI: 10.1016/0014-5793(96)00804-6
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000C32
- to stream Istex, to step Curation: 000814
- to stream Istex, to step Checkpoint: 000832
- to stream Main, to step Merge: 001030
- to stream Main, to step Curation: 000F91
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Neurotoxic glutamate treatment of cultured cerebellar granule cells induces Ca2+-dependent collapse of mitochondrial membrane potential and ultrastructural alterations of mitochondria</title>
<author><name sortKey="Isaev, Nikolaj K" sort="Isaev, Nikolaj K" uniqKey="Isaev N" first="Nikolaj K." last="Isaev">Nikolaj K. Isaev</name>
</author>
<author><name sortKey="Zorov, Dmitry B" sort="Zorov, Dmitry B" uniqKey="Zorov D" first="Dmitry B." last="Zorov">Dmitry B. Zorov</name>
</author>
<author><name sortKey="Stelmashook, Elena V" sort="Stelmashook, Elena V" uniqKey="Stelmashook E" first="Elena V." last="Stelmashook">Elena V. Stelmashook</name>
</author>
<author><name sortKey="Uzbekov, Rustem E" sort="Uzbekov, Rustem E" uniqKey="Uzbekov R" first="Rustem E." last="Uzbekov">Rustem E. Uzbekov</name>
</author>
<author><name sortKey="Kozhemyakin, Maxim B" sort="Kozhemyakin, Maxim B" uniqKey="Kozhemyakin M" first="Maxim B." last="Kozhemyakin">Maxim B. Kozhemyakin</name>
</author>
<author><name sortKey="Victorov, Ilya V" sort="Victorov, Ilya V" uniqKey="Victorov I" first="Ilya V." last="Victorov">Ilya V. Victorov</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:F1127E136A67A205D23722D1F7D9FD17CE133CC8</idno>
<date when="1996" year="1996">1996</date>
<idno type="doi">10.1016/0014-5793(96)00804-6</idno>
<idno type="url">https://api.istex.fr/document/F1127E136A67A205D23722D1F7D9FD17CE133CC8/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000C32</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C32</idno>
<idno type="wicri:Area/Istex/Curation">000814</idno>
<idno type="wicri:Area/Istex/Checkpoint">000832</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000832</idno>
<idno type="wicri:doubleKey">0014-5793:1996:Isaev N:neurotoxic:glutamate:treatment</idno>
<idno type="wicri:Area/Main/Merge">001030</idno>
<idno type="wicri:Area/Main/Curation">000F91</idno>
<idno type="wicri:Area/Main/Exploration">000F91</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Neurotoxic glutamate treatment of cultured cerebellar granule cells induces Ca2+-dependent collapse of mitochondrial membrane potential and ultrastructural alterations of mitochondria</title>
<author><name sortKey="Isaev, Nikolaj K" sort="Isaev, Nikolaj K" uniqKey="Isaev N" first="Nikolaj K." last="Isaev">Nikolaj K. Isaev</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Brain Research Institute, Russian Academy of Medical Sciences, p. Obuha 5, 103064, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zorov, Dmitry B" sort="Zorov, Dmitry B" uniqKey="Zorov D" first="Dmitry B." last="Zorov">Dmitry B. Zorov</name>
<affiliation wicri:level="4"><country xml:lang="fr">Russie</country>
<wicri:regionArea>A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 117292, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
<orgName type="university">Université d'État de Moscou</orgName>
</affiliation>
</author>
<author><name sortKey="Stelmashook, Elena V" sort="Stelmashook, Elena V" uniqKey="Stelmashook E" first="Elena V." last="Stelmashook">Elena V. Stelmashook</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Brain Research Institute, Russian Academy of Medical Sciences, p. Obuha 5, 103064, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Uzbekov, Rustem E" sort="Uzbekov, Rustem E" uniqKey="Uzbekov R" first="Rustem E." last="Uzbekov">Rustem E. Uzbekov</name>
<affiliation wicri:level="4"><country xml:lang="fr">Russie</country>
<wicri:regionArea>A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 117292, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
<orgName type="university">Université d'État de Moscou</orgName>
</affiliation>
</author>
<author><name sortKey="Kozhemyakin, Maxim B" sort="Kozhemyakin, Maxim B" uniqKey="Kozhemyakin M" first="Maxim B." last="Kozhemyakin">Maxim B. Kozhemyakin</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Brain Research Institute, Russian Academy of Medical Sciences, p. Obuha 5, 103064, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Victorov, Ilya V" sort="Victorov, Ilya V" uniqKey="Victorov I" first="Ilya V." last="Victorov">Ilya V. Victorov</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Brain Research Institute, Russian Academy of Medical Sciences, p. Obuha 5, 103064, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">FEBS Letters</title>
<title level="j" type="abbrev">FEBS</title>
<idno type="ISSN">0014-5793</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1996">1996</date>
<biblScope unit="volume">392</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="143">143</biblScope>
<biblScope unit="page" to="147">147</biblScope>
</imprint>
<idno type="ISSN">0014-5793</idno>
</series>
<idno type="istex">F1127E136A67A205D23722D1F7D9FD17CE133CC8</idno>
<idno type="DOI">10.1016/0014-5793(96)00804-6</idno>
<idno type="PII">0014-5793(96)00804-6</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0014-5793</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Rhodamine 123 staining and electron microscopy were used to reveal a correlation between the ultrastructural and functional state of cultured cerebellar granule cells after short glutamate treatment. Glutamate exposure (15 min, 100 μM) in Mg2+-free solution caused considerable ultrastructural alterations in a granule cell: clumping of the chromatin, swelling of the endoplasmic reticulum and mitochondria, and disruption of the mitochondrial cristae. After glutamate treatment, the mitochondria of the neurons lost their ability to sequester rhodamine 123. Both the N-ethyl-d-aspartate receptor channel blocker MK-801 (30 μM) and cobalt chloride (2 mM) prevented the deteriorative effects of glutamate. These data suggest that glutamate-induced Ca2+ overload of the neurons can lead to non-specific permeability of the inner mitochondrial membrane, resulting in neuronal death.</div>
</front>
</TEI>
<affiliations><list><country><li>Russie</li>
</country>
<region><li>District fédéral central</li>
</region>
<settlement><li>Moscou</li>
</settlement>
<orgName><li>Université d'État de Moscou</li>
</orgName>
</list>
<tree><country name="Russie"><region name="District fédéral central"><name sortKey="Isaev, Nikolaj K" sort="Isaev, Nikolaj K" uniqKey="Isaev N" first="Nikolaj K." last="Isaev">Nikolaj K. Isaev</name>
</region>
<name sortKey="Kozhemyakin, Maxim B" sort="Kozhemyakin, Maxim B" uniqKey="Kozhemyakin M" first="Maxim B." last="Kozhemyakin">Maxim B. Kozhemyakin</name>
<name sortKey="Stelmashook, Elena V" sort="Stelmashook, Elena V" uniqKey="Stelmashook E" first="Elena V." last="Stelmashook">Elena V. Stelmashook</name>
<name sortKey="Uzbekov, Rustem E" sort="Uzbekov, Rustem E" uniqKey="Uzbekov R" first="Rustem E." last="Uzbekov">Rustem E. Uzbekov</name>
<name sortKey="Victorov, Ilya V" sort="Victorov, Ilya V" uniqKey="Victorov I" first="Ilya V." last="Victorov">Ilya V. Victorov</name>
<name sortKey="Zorov, Dmitry B" sort="Zorov, Dmitry B" uniqKey="Zorov D" first="Dmitry B." last="Zorov">Dmitry B. Zorov</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F91 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000F91 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Terre |area= CobaltMaghrebV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:F1127E136A67A205D23722D1F7D9FD17CE133CC8 |texte= Neurotoxic glutamate treatment of cultured cerebellar granule cells induces Ca2+-dependent collapse of mitochondrial membrane potential and ultrastructural alterations of mitochondria }}
This area was generated with Dilib version V0.6.32. |