Influence of the rpoS gene and pre-adaptation in seawater on the tolerance to heavy metals of Salmonella enterica serovar Typhimurium
Identifieur interne : 000450 ( Main/Exploration ); précédent : 000449; suivant : 000451Influence of the rpoS gene and pre-adaptation in seawater on the tolerance to heavy metals of Salmonella enterica serovar Typhimurium
Auteurs : Rihab Lagha [Tunisie] ; Fethi Ben Abdallah [Tunisie] ; Amina Bakhrouf [Tunisie]Source :
- Annals of microbiology [ 1590-4261 ] ; 2012.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Métal lourd.
English descriptors
- KwdEn :
Abstract
Salmonella is an international food-borne pathogen disseminated widely in seawater that regularly causes large outbreaks of food poisoning. In this study, we investigated the effect of starvation on the survival ability of Salmonella enterica serovar Typhimurium rpoS+ and its mutant rpoS- after a 6-month incubation in seawater microcosms using a colorimetric XTT assay and colony forming units (CFU). The effect of pre-adaptation in seawater and the rpoS gene on the tolerance to heavy metals of this microorganism was also investigated. Our results showed that the number of cells decreased by ∼5 log units for the strain rpoS+ and by∼ 5.5 log units for the mutant rpoS- after this period of stress. At the end of the experiments, cell viability was around of 20% and 10% for the wild type and mutant, respectively. We also revealed that the minimal inhibitory concentration values and cell viability under zinc, cobalt, mercury, chromium and cadmium heavy metals were decreased for the two starved strains.
Affiliations:
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de I'Environnement et des Produits, Faculté de Pharmacie, Rue Avicenne</s1><s2>Monastir 5000</s2><s3>TUN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Tunisie</country><wicri:noRegion>Monastir 5000</wicri:noRegion></affiliation></author><author><name sortKey="Abdallah, Fethi Ben" sort="Abdallah, Fethi Ben" uniqKey="Abdallah F" first="Fethi Ben" last="Abdallah">Fethi Ben Abdallah</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratoire d'Analyse, Traitement et Valorisation des Polluants de I'Environnement et des Produits, Faculté de Pharmacie, Rue Avicenne</s1><s2>Monastir 5000</s2><s3>TUN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Tunisie</country><wicri:noRegion>Monastir 5000</wicri:noRegion></affiliation></author><author><name sortKey="Bakhrouf, Amina" sort="Bakhrouf, Amina" uniqKey="Bakhrouf A" first="Amina" last="Bakhrouf">Amina Bakhrouf</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratoire d'Analyse, Traitement et Valorisation des Polluants de I'Environnement et des Produits, Faculté de Pharmacie, Rue Avicenne</s1><s2>Monastir 5000</s2><s3>TUN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Tunisie</country><wicri:noRegion>Monastir 5000</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Annals of microbiology</title><title level="j" type="abbreviated">Ann. microbiol.</title><idno type="ISSN">1590-4261</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Annals of microbiology</title><title level="j" type="abbreviated">Ann. microbiol.</title><idno type="ISSN">1590-4261</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation</term><term>Gene</term><term>Heavy metal</term><term>Salmonella typhimurium</term><term>Seawater</term><term>Survival</term><term>Tolerance</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Gène</term><term>Adaptation</term><term>Eau mer</term><term>Tolérance</term><term>Métal lourd</term><term>Salmonella typhimurium</term><term>Survie</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Métal lourd</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Salmonella is an international food-borne pathogen disseminated widely in seawater that regularly causes large outbreaks of food poisoning. In this study, we investigated the effect of starvation on the survival ability of Salmonella enterica serovar Typhimurium rpoS<sup>+</sup> and its mutant rpoS<sup>-</sup> after a 6-month incubation in seawater microcosms using a colorimetric XTT assay and colony forming units (CFU). The effect of pre-adaptation in seawater and the rpoS gene on the tolerance to heavy metals of this microorganism was also investigated. Our results showed that the number of cells decreased by ∼5 log units for the strain rpoS<sup>+ </sup>and by∼ 5.5 log units for the mutant rpoS<sup>-</sup> after this period of stress. At the end of the experiments, cell viability was around of 20% and 10% for the wild type and mutant, respectively. We also revealed that the minimal inhibitory concentration values and cell viability under zinc, cobalt, mercury, chromium and cadmium heavy metals were decreased for the two starved strains.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><country name="Tunisie"><noRegion><name sortKey="Lagha, Rihab" sort="Lagha, Rihab" uniqKey="Lagha R" first="Rihab" last="Lagha">Rihab Lagha</name></noRegion><name sortKey="Abdallah, Fethi Ben" sort="Abdallah, Fethi Ben" uniqKey="Abdallah F" first="Fethi Ben" last="Abdallah">Fethi Ben Abdallah</name><name sortKey="Bakhrouf, Amina" sort="Bakhrouf, Amina" uniqKey="Bakhrouf A" first="Amina" last="Bakhrouf">Amina Bakhrouf</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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