Time course of axotomy-induced apoptotic cell death in facial motoneurons of neonatal wild type and bcl-2 transgenic mice
Identifieur interne : 000C55 ( Istex/Curation ); précédent : 000C54; suivant : 000C56Time course of axotomy-induced apoptotic cell death in facial motoneurons of neonatal wild type and bcl-2 transgenic mice
Auteurs : F. De Bilbao [Suisse] ; M. Dubois-Dauphin [Suisse]Source :
- Neuroscience [ 0306-4522 ] ; 1996.
English descriptors
- KwdEn :
Abstract
Abstract: In neonatal animals, axotomy of facial motoneurons induces cell death. Using the TUNEL technique, which labelled apoptotic DNA breaks in situ, the kinetics of motoneuron death were studied. Lesion of the right facial nerve were performed on two-day-old mice. Then, animals were perfused 8, 12, 16, 20, 24, 28, 32, 48, 72 and 120 h after the lesion. Our results provide direct evidence that, following an axotomy, facial motoneurons die through an apoptotic process. We showed that apoptotic neurons can be detected as early as 16 h after the lesion. Facial motoneurons die within 120 h, with a peak observed 28 h after the lesion. The kinetics of appearance of apoptotic cells were correlated with the loss of Cresyl Violet-stained motoneurons. Furthermore, labelled cells were observed in the contralateral side of the lesion, suggesting that spontaneous apoptotic cell death occurs during the postnatal period. The same study was performed on transgenic mice overexpressing the proto-oncogene bcl-2, a gene repressor of cell death. In these mice, no TUNEL-labelled cells were detected on the lesioned and unlesioned sides. In vivo, Bcl-2 may protect motoneurons from apoptotic death following axotomy and during naturally occurring cell death. These results suggest that these two types of cell death may occur via the same mechanism.
Url:
DOI: 10.1016/0306-4522(95)00505-6
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001129
Links to Exploration step
ISTEX:82132C6BC45A5FE5E1174C149FA03B1457D2FFC1Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Time course of axotomy-induced apoptotic cell death in facial motoneurons of neonatal wild type and bcl-2 transgenic mice</title><author><name sortKey="De Bilbao, F" sort="De Bilbao, F" uniqKey="De Bilbao F" first="F." last="De Bilbao">F. De Bilbao</name><affiliation wicri:level="1"><mods:affiliation>Department of Physiology, Centre Medical Universitaire, 1 rue Michel Servet, 1211 Geneva 4, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Physiology, Centre Medical Universitaire, 1 rue Michel Servet, 1211 Geneva 4</wicri:regionArea></affiliation></author><author><name sortKey="Dubois Dauphin, M" sort="Dubois Dauphin, M" uniqKey="Dubois Dauphin M" first="M." last="Dubois-Dauphin">M. Dubois-Dauphin</name><affiliation wicri:level="1"><mods:affiliation>Department of Physiology, Centre Medical Universitaire, 1 rue Michel Servet, 1211 Geneva 4, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Physiology, Centre Medical Universitaire, 1 rue Michel Servet, 1211 Geneva 4</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:82132C6BC45A5FE5E1174C149FA03B1457D2FFC1</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1016/0306-4522(95)00505-6</idno><idno type="url">https://api.istex.fr/document/82132C6BC45A5FE5E1174C149FA03B1457D2FFC1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001129</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001129</idno><idno type="wicri:Area/Istex/Curation">000C55</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Time course of axotomy-induced apoptotic cell death in facial motoneurons of neonatal wild type and bcl-2 transgenic mice</title><author><name sortKey="De Bilbao, F" sort="De Bilbao, F" uniqKey="De Bilbao F" first="F." last="De Bilbao">F. De Bilbao</name><affiliation wicri:level="1"><mods:affiliation>Department of Physiology, Centre Medical Universitaire, 1 rue Michel Servet, 1211 Geneva 4, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Physiology, Centre Medical Universitaire, 1 rue Michel Servet, 1211 Geneva 4</wicri:regionArea></affiliation></author><author><name sortKey="Dubois Dauphin, M" sort="Dubois Dauphin, M" uniqKey="Dubois Dauphin M" first="M." last="Dubois-Dauphin">M. Dubois-Dauphin</name><affiliation wicri:level="1"><mods:affiliation>Department of Physiology, Centre Medical Universitaire, 1 rue Michel Servet, 1211 Geneva 4, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Physiology, Centre Medical Universitaire, 1 rue Michel Servet, 1211 Geneva 4</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Neuroscience</title><title level="j" type="abbrev">NSC</title><idno type="ISSN">0306-4522</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">71</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="1111">1111</biblScope><biblScope unit="page" to="1119">1119</biblScope></imprint><idno type="ISSN">0306-4522</idno></series><idno type="istex">82132C6BC45A5FE5E1174C149FA03B1457D2FFC1</idno><idno type="DOI">10.1016/0306-4522(95)00505-6</idno><idno type="PII">0306-4522(95)00505-6</idno><idno type="ArticleID">95005056</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0306-4522</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>PBS</term><term>TUNEL method</term><term>TdT</term><term>degeneration</term><term>neuronal cell death</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In neonatal animals, axotomy of facial motoneurons induces cell death. Using the TUNEL technique, which labelled apoptotic DNA breaks in situ, the kinetics of motoneuron death were studied. Lesion of the right facial nerve were performed on two-day-old mice. Then, animals were perfused 8, 12, 16, 20, 24, 28, 32, 48, 72 and 120 h after the lesion. Our results provide direct evidence that, following an axotomy, facial motoneurons die through an apoptotic process. We showed that apoptotic neurons can be detected as early as 16 h after the lesion. Facial motoneurons die within 120 h, with a peak observed 28 h after the lesion. The kinetics of appearance of apoptotic cells were correlated with the loss of Cresyl Violet-stained motoneurons. Furthermore, labelled cells were observed in the contralateral side of the lesion, suggesting that spontaneous apoptotic cell death occurs during the postnatal period. The same study was performed on transgenic mice overexpressing the proto-oncogene bcl-2, a gene repressor of cell death. In these mice, no TUNEL-labelled cells were detected on the lesioned and unlesioned sides. In vivo, Bcl-2 may protect motoneurons from apoptotic death following axotomy and during naturally occurring cell death. These results suggest that these two types of cell death may occur via the same mechanism.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C55 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000C55 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= CobaltMaghrebV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:82132C6BC45A5FE5E1174C149FA03B1457D2FFC1
|texte= Time course of axotomy-induced apoptotic cell death in facial motoneurons of neonatal wild type and bcl-2 transgenic mice
}}
| This area was generated with Dilib version V0.6.32. |  |