Interaction of metallopyrazoliumylporphyrins with calf thymus DNA
Identifieur interne : 001564 ( Istex/Corpus ); précédent : 001563; suivant : 001565Interaction of metallopyrazoliumylporphyrins with calf thymus DNA
Auteurs : Daryono H. Tjahjono ; Shunsuke Mima ; Takehiro Akutsu ; Naoki Yoshioka ; Hidenari InoueSource :
- Journal of Inorganic Biochemistry [ 0162-0134 ] ; 2001.
Abstract
The interaction of transition metal complexes of cationic porphyrins bearing five membered rings, meso-tetrakis(1,2-dimethylpyrazolium-4-yl)porphyrin (MPzP, M=MnIII, NiII, CuII or ZnII), with calf thymus DNA (ctDNA) has been studied. Metalloporphyrins NiPzP and CuPzP are intercalated into the 5′GC3′ step of ctDNA. MnPzP is bound edge-on at the 5′TA3′ step of the minor groove of ctDNA, while ZnPzP is bound face-on at the 5′TA3′ step of the major groove of ctDNA. The binding constants of the metalloporphyrins to ctDNA range from 1.05×105 to 2.66×106 M−1 and are comparable to those of other reported cationic porphyrins. The binding process of the metallopyrazoliumylporphyrins to ctDNA is endothermic and entropically driven. These results have revealed that the kind of central metal ions of metalloporphyrins influences the binding characteristics of the porphyrin to DNA.
Url:
DOI: 10.1016/S0162-0134(01)00186-6
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Tjahjono</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: c02935@educ.cc.keio.ac.jp</mods:affiliation></affiliation></author><author><name sortKey="Mima, Shunsuke" sort="Mima, Shunsuke" uniqKey="Mima S" first="Shunsuke" last="Mima">Shunsuke Mima</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation></author><author><name sortKey="Akutsu, Takehiro" sort="Akutsu, Takehiro" uniqKey="Akutsu T" first="Takehiro" last="Akutsu">Takehiro Akutsu</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation></author><author><name sortKey="Yoshioka, Naoki" sort="Yoshioka, Naoki" uniqKey="Yoshioka N" first="Naoki" last="Yoshioka">Naoki Yoshioka</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation></author><author><name sortKey="Inoue, Hidenari" sort="Inoue, Hidenari" uniqKey="Inoue H" first="Hidenari" last="Inoue">Hidenari Inoue</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1642D1218149EBED06670529AD5D13DF1D549BD5</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1016/S0162-0134(01)00186-6</idno><idno type="url">https://api.istex.fr/document/1642D1218149EBED06670529AD5D13DF1D549BD5/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001564</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001564</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Interaction of metallopyrazoliumylporphyrins with calf thymus DNA</title><author><name sortKey="Tjahjono, Daryono H" sort="Tjahjono, Daryono H" uniqKey="Tjahjono D" first="Daryono H" last="Tjahjono">Daryono H. Tjahjono</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: c02935@educ.cc.keio.ac.jp</mods:affiliation></affiliation></author><author><name sortKey="Mima, Shunsuke" sort="Mima, Shunsuke" uniqKey="Mima S" first="Shunsuke" last="Mima">Shunsuke Mima</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation></author><author><name sortKey="Akutsu, Takehiro" sort="Akutsu, Takehiro" uniqKey="Akutsu T" first="Takehiro" last="Akutsu">Takehiro Akutsu</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation></author><author><name sortKey="Yoshioka, Naoki" sort="Yoshioka, Naoki" uniqKey="Yoshioka N" first="Naoki" last="Yoshioka">Naoki Yoshioka</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation></author><author><name sortKey="Inoue, Hidenari" sort="Inoue, Hidenari" uniqKey="Inoue H" first="Hidenari" last="Inoue">Hidenari Inoue</name><affiliation><mods:affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Inorganic Biochemistry</title><title level="j" type="abbrev">JIB</title><idno type="ISSN">0162-0134</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">85</biblScope><biblScope unit="issue">2–3</biblScope><biblScope unit="page" from="219">219</biblScope><biblScope unit="page" to="228">228</biblScope></imprint><idno type="ISSN">0162-0134</idno></series><idno type="istex">1642D1218149EBED06670529AD5D13DF1D549BD5</idno><idno type="DOI">10.1016/S0162-0134(01)00186-6</idno><idno type="PII">S0162-0134(01)00186-6</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0162-0134</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The interaction of transition metal complexes of cationic porphyrins bearing five membered rings, meso-tetrakis(1,2-dimethylpyrazolium-4-yl)porphyrin (MPzP, M=MnIII, NiII, CuII or ZnII), with calf thymus DNA (ctDNA) has been studied. Metalloporphyrins NiPzP and CuPzP are intercalated into the 5′GC3′ step of ctDNA. MnPzP is bound edge-on at the 5′TA3′ step of the minor groove of ctDNA, while ZnPzP is bound face-on at the 5′TA3′ step of the major groove of ctDNA. The binding constants of the metalloporphyrins to ctDNA range from 1.05×105 to 2.66×106 M−1 and are comparable to those of other reported cationic porphyrins. The binding process of the metallopyrazoliumylporphyrins to ctDNA is endothermic and entropically driven. These results have revealed that the kind of central metal ions of metalloporphyrins influences the binding characteristics of the porphyrin to DNA.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Daryono H Tjahjono</name><affiliations><json:string>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</json:string><json:string>E-mail: c02935@educ.cc.keio.ac.jp</json:string></affiliations></json:item><json:item><name>Shunsuke Mima</name><affiliations><json:string>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</json:string></affiliations></json:item><json:item><name>Takehiro Akutsu</name><affiliations><json:string>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</json:string></affiliations></json:item><json:item><name>Naoki Yoshioka</name><affiliations><json:string>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</json:string></affiliations></json:item><json:item><name>Hidenari Inoue</name><affiliations><json:string>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Cationic pyrazoliumylporphyrin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Metalloporphyrins</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Calf thymus DNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Porphyrin–DNA interaction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Thermodynamic parameters</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>The interaction of transition metal complexes of cationic porphyrins bearing five membered rings, meso-tetrakis(1,2-dimethylpyrazolium-4-yl)porphyrin (MPzP, M=MnIII, NiII, CuII or ZnII), with calf thymus DNA (ctDNA) has been studied. Metalloporphyrins NiPzP and CuPzP are intercalated into the 5′GC3′ step of ctDNA. MnPzP is bound edge-on at the 5′TA3′ step of the minor groove of ctDNA, while ZnPzP is bound face-on at the 5′TA3′ step of the major groove of ctDNA. The binding constants of the metalloporphyrins to ctDNA range from 1.05×105 to 2.66×106 M−1 and are comparable to those of other reported cationic porphyrins. The binding process of the metallopyrazoliumylporphyrins to ctDNA is endothermic and entropically driven. These results have revealed that the kind of central metal ions of metalloporphyrins influences the binding characteristics of the porphyrin to DNA.</abstract><qualityIndicators><score>6.536</score><pdfVersion>1.2</pdfVersion><pdfPageSize>598 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>879</abstractCharCount><pdfWordCount>6235</pdfWordCount><pdfCharCount>34727</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>128</abstractWordCount></qualityIndicators><title>Interaction of metallopyrazoliumylporphyrins with calf thymus DNA</title><pii><json:string>S0162-0134(01)00186-6</json:string></pii><genre><json:string>research-article</json:string></genre><serie><language><json:string>unknown</json:string></language><title>Chapter 46</title></serie><host><volume>85</volume><pii><json:string>S0162-0134(00)X0252-8</json:string></pii><pages><last>228</last><first>219</first></pages><issn><json:string>0162-0134</json:string></issn><issue>2–3</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Journal of Inorganic Biochemistry</title><publicationDate>2001</publicationDate></host><categories><wos><json:string>CHEMISTRY, INORGANIC & NUCLEAR</json:string><json:string>BIOCHEMISTRY & MOLECULAR BIOLOGY</json:string></wos></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1016/S0162-0134(01)00186-6</json:string></doi><id>1642D1218149EBED06670529AD5D13DF1D549BD5</id><score>0.038330868</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/1642D1218149EBED06670529AD5D13DF1D549BD5/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/1642D1218149EBED06670529AD5D13DF1D549BD5/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/1642D1218149EBED06670529AD5D13DF1D549BD5/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Interaction of metallopyrazoliumylporphyrins with calf thymus DNA</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2001 Elsevier Science B.V.</p></availability><date>2001</date></publicationStmt><notesStmt><note type="content">Fig. 1: Structure of metallotetrakispyrazoliumylporphyrins, [MPzP]I4.</note><note type="content">Fig. 2: Visible, CD and MCD spectra of free (solid line) and ctDNA-bound (dashed line) [MnPzP]I4 in phosphate buffer (pH 6.8, μ=0.2 M) at R (=[porphyrin]/[DNA])=0.02.</note><note type="content">Fig. 3: Visible, CD and MCD spectra of free (solid line) and ctDNA-bound (dashed line) [NiPzP]I4 in phosphate buffer (pH 6.8, μ=0.2 M) at R (=[porphyrin]/[DNA])=0.02.</note><note type="content">Fig. 4: Visible, CD and MCD spectra of free (solid line) and ctDNA-bound (dashed line) [CuPzP]I4 in phosphate buffer (pH 6.8, μ=0.2 M) at R (=[porphyrin]/[DNA])=0.02.</note><note type="content">Fig. 5: Visible, CD and MCD spectra of free (solid line) and ctDNA-bound (dashed line) [ZnPzP]I4 in phosphate buffer (pH 6.8, μ=0.2 M) at R (=[porphyrin]/[DNA])=0.02.</note><note type="content">Fig. 6: Plots of the relative viscosity of ctDNA versus R value of [MnPzP]I4 (triangles), [NiPzP]I4 (diamonds), [CuPzP]I4 (squares) and [ZnPzP]I4 (circles) in phosphate buffer (pH 6.8, μ=0.2 M) at 30±1°C.</note><note type="content">Fig. 7: Plots of the increase in ctDNA melting temperature (ΔTm) versus R values of [MnPzP]I4 (triangles), [NiPzP]I4 (diamonds), [CuPzP]I4 (squares) and [ZnPzP]I4 (circles) in phosphate buffer (pH 6.8, μ=0.2 M).</note><note type="content">Table 1: Thermodynamic parameters of interactions between [MPzP]I4 and ctDNAa</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Interaction of metallopyrazoliumylporphyrins with calf thymus DNA</title><author xml:id="author-1"><persName><forename type="first">Daryono H</forename><surname>Tjahjono</surname></persName><email>c02935@educ.cc.keio.ac.jp</email><note type="biography">On sabbatical leave from Department of Pharmacy, Bandung Institute of Technology, Jalan Ganesha 10, Bandung 40132, Indonesia.</note><note type="correspondence"><p>Corresponding author</p></note><affiliation>On sabbatical leave from Department of Pharmacy, Bandung Institute of Technology, Jalan Ganesha 10, Bandung 40132, Indonesia.</affiliation><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation></author><author xml:id="author-2"><persName><forename type="first">Shunsuke</forename><surname>Mima</surname></persName><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation></author><author xml:id="author-3"><persName><forename type="first">Takehiro</forename><surname>Akutsu</surname></persName><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation></author><author xml:id="author-4"><persName><forename type="first">Naoki</forename><surname>Yoshioka</surname></persName><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation></author><author xml:id="author-5"><persName><forename type="first">Hidenari</forename><surname>Inoue</surname></persName><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation></author></analytic><monogr><title level="j">Journal of Inorganic Biochemistry</title><title level="j" type="abbrev">JIB</title><idno type="pISSN">0162-0134</idno><idno type="PII">S0162-0134(00)X0252-8</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001"></date><biblScope unit="volume">85</biblScope><biblScope unit="issue">2–3</biblScope><biblScope unit="page" from="219">219</biblScope><biblScope unit="page" to="228">228</biblScope></imprint></monogr><idno type="istex">1642D1218149EBED06670529AD5D13DF1D549BD5</idno><idno type="DOI">10.1016/S0162-0134(01)00186-6</idno><idno type="PII">S0162-0134(01)00186-6</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The interaction of transition metal complexes of cationic porphyrins bearing five membered rings, meso-tetrakis(1,2-dimethylpyrazolium-4-yl)porphyrin (MPzP, M=MnIII, NiII, CuII or ZnII), with calf thymus DNA (ctDNA) has been studied. Metalloporphyrins NiPzP and CuPzP are intercalated into the 5′GC3′ step of ctDNA. MnPzP is bound edge-on at the 5′TA3′ step of the minor groove of ctDNA, while ZnPzP is bound face-on at the 5′TA3′ step of the major groove of ctDNA. The binding constants of the metalloporphyrins to ctDNA range from 1.05×105 to 2.66×106 M−1 and are comparable to those of other reported cationic porphyrins. The binding process of the metallopyrazoliumylporphyrins to ctDNA is endothermic and entropically driven. These results have revealed that the kind of central metal ions of metalloporphyrins influences the binding characteristics of the porphyrin to DNA.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Cationic pyrazoliumylporphyrin</term></item><item><term>Metalloporphyrins</term></item><item><term>Calf thymus DNA</term></item><item><term>Porphyrin–DNA interaction</term></item><item><term>Thermodynamic parameters</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2001-01-17">Modified</change><change when="2001">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/1642D1218149EBED06670529AD5D13DF1D549BD5/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="GR1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="GR2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="GR3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="GR4"></istex:entity><istex:entity SYSTEM="gr5" NDATA="IMAGE" name="GR5"></istex:entity><istex:entity SYSTEM="gr6" NDATA="IMAGE" name="GR6"></istex:entity><istex:entity SYSTEM="gr7" NDATA="IMAGE" name="GR7"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JIB</jid><aid>6576</aid><ce:pii>S0162-0134(01)00186-6</ce:pii><ce:doi>10.1016/S0162-0134(01)00186-6</ce:doi><ce:copyright type="full-transfer" year="2001">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>Interaction of metallopyrazoliumylporphyrins with calf thymus DNA</ce:title><ce:author-group><ce:author><ce:given-name>Daryono H</ce:given-name><ce:surname>Tjahjono</ce:surname><ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref><ce:e-address>c02935@educ.cc.keio.ac.jp</ce:e-address></ce:author><ce:author><ce:given-name>Shunsuke</ce:given-name><ce:surname>Mima</ce:surname></ce:author><ce:author><ce:given-name>Takehiro</ce:given-name><ce:surname>Akutsu</ce:surname></ce:author><ce:author><ce:given-name>Naoki</ce:given-name><ce:surname>Yoshioka</ce:surname></ce:author><ce:author><ce:given-name>Hidenari</ce:given-name><ce:surname>Inoue</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>On sabbatical leave from Department of Pharmacy, Bandung Institute of Technology, Jalan Ganesha 10, Bandung 40132, Indonesia.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="23" month="10" year="2000"></ce:date-received><ce:date-revised day="17" month="1" year="2001"></ce:date-revised><ce:date-accepted day="22" month="1" year="2001"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The interaction of transition metal complexes of cationic porphyrins bearing five membered rings, <ce:italic>meso</ce:italic>-tetrakis(1,2-dimethylpyrazolium-4-yl)porphyrin (MPzP, M=Mn<ce:sup>III</ce:sup>, Ni<ce:sup>II</ce:sup>, Cu<ce:sup>II</ce:sup> or Zn<ce:sup>II</ce:sup>), with calf thymus DNA (ctDNA) has been studied. Metalloporphyrins NiPzP and CuPzP are intercalated into the 5′GC3′ step of ctDNA. MnPzP is bound edge-on at the 5′TA3′ step of the minor groove of ctDNA, while ZnPzP is bound face-on at the 5′TA3′ step of the major groove of ctDNA. The binding constants of the metalloporphyrins to ctDNA range from 1.05×10<ce:sup>5</ce:sup> to 2.66×10<ce:sup>6</ce:sup> M<ce:sup>−1</ce:sup> and are comparable to those of other reported cationic porphyrins. The binding process of the metallopyrazoliumylporphyrins to ctDNA is endothermic and entropically driven. These results have revealed that the kind of central metal ions of metalloporphyrins influences the binding characteristics of the porphyrin to DNA.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Cationic pyrazoliumylporphyrin</ce:text></ce:keyword><ce:keyword><ce:text>Metalloporphyrins</ce:text></ce:keyword><ce:keyword><ce:text>Calf thymus DNA</ce:text></ce:keyword><ce:keyword><ce:text>Porphyrin–DNA interaction</ce:text></ce:keyword><ce:keyword><ce:text>Thermodynamic parameters</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Interaction of metallopyrazoliumylporphyrins with calf thymus DNA</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Interaction of metallopyrazoliumylporphyrins with calf thymus DNA</title></titleInfo><name type="personal"><namePart type="given">Daryono H</namePart><namePart type="family">Tjahjono</namePart><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation><affiliation>E-mail: c02935@educ.cc.keio.ac.jp</affiliation><description>Corresponding author</description><description>On sabbatical leave from Department of Pharmacy, Bandung Institute of Technology, Jalan Ganesha 10, Bandung 40132, Indonesia.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shunsuke</namePart><namePart type="family">Mima</namePart><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Takehiro</namePart><namePart type="family">Akutsu</namePart><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Naoki</namePart><namePart type="family">Yoshioka</namePart><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hidenari</namePart><namePart type="family">Inoue</namePart><affiliation>Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued><dateModified encoding="w3cdtf">2001-01-17</dateModified><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The interaction of transition metal complexes of cationic porphyrins bearing five membered rings, meso-tetrakis(1,2-dimethylpyrazolium-4-yl)porphyrin (MPzP, M=MnIII, NiII, CuII or ZnII), with calf thymus DNA (ctDNA) has been studied. Metalloporphyrins NiPzP and CuPzP are intercalated into the 5′GC3′ step of ctDNA. MnPzP is bound edge-on at the 5′TA3′ step of the minor groove of ctDNA, while ZnPzP is bound face-on at the 5′TA3′ step of the major groove of ctDNA. The binding constants of the metalloporphyrins to ctDNA range from 1.05×105 to 2.66×106 M−1 and are comparable to those of other reported cationic porphyrins. The binding process of the metallopyrazoliumylporphyrins to ctDNA is endothermic and entropically driven. These results have revealed that the kind of central metal ions of metalloporphyrins influences the binding characteristics of the porphyrin to DNA.</abstract><note type="content">Fig. 1: Structure of metallotetrakispyrazoliumylporphyrins, [MPzP]I4.</note><note type="content">Fig. 2: Visible, CD and MCD spectra of free (solid line) and ctDNA-bound (dashed line) [MnPzP]I4 in phosphate buffer (pH 6.8, μ=0.2 M) at R (=[porphyrin]/[DNA])=0.02.</note><note type="content">Fig. 3: Visible, CD and MCD spectra of free (solid line) and ctDNA-bound (dashed line) [NiPzP]I4 in phosphate buffer (pH 6.8, μ=0.2 M) at R (=[porphyrin]/[DNA])=0.02.</note><note type="content">Fig. 4: Visible, CD and MCD spectra of free (solid line) and ctDNA-bound (dashed line) [CuPzP]I4 in phosphate buffer (pH 6.8, μ=0.2 M) at R (=[porphyrin]/[DNA])=0.02.</note><note type="content">Fig. 5: Visible, CD and MCD spectra of free (solid line) and ctDNA-bound (dashed line) [ZnPzP]I4 in phosphate buffer (pH 6.8, μ=0.2 M) at R (=[porphyrin]/[DNA])=0.02.</note><note type="content">Fig. 6: Plots of the relative viscosity of ctDNA versus R value of [MnPzP]I4 (triangles), [NiPzP]I4 (diamonds), [CuPzP]I4 (squares) and [ZnPzP]I4 (circles) in phosphate buffer (pH 6.8, μ=0.2 M) at 30±1°C.</note><note type="content">Fig. 7: Plots of the increase in ctDNA melting temperature (ΔTm) versus R values of [MnPzP]I4 (triangles), [NiPzP]I4 (diamonds), [CuPzP]I4 (squares) and [ZnPzP]I4 (circles) in phosphate buffer (pH 6.8, μ=0.2 M).</note><note type="content">Table 1: Thermodynamic parameters of interactions between [MPzP]I4 and ctDNAa</note><subject><genre>Keywords</genre><topic>Cationic pyrazoliumylporphyrin</topic><topic>Metalloporphyrins</topic><topic>Calf thymus DNA</topic><topic>Porphyrin–DNA interaction</topic><topic>Thermodynamic parameters</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Inorganic Biochemistry</title></titleInfo><titleInfo type="abbreviated"><title>JIB</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">200106</dateIssued></originInfo><identifier type="ISSN">0162-0134</identifier><identifier type="PII">S0162-0134(00)X0252-8</identifier><part><date>200106</date><detail type="volume"><number>85</number><caption>vol.</caption></detail><detail type="issue"><number>2–3</number><caption>no.</caption></detail><extent unit="issue pages"><start>79</start><end>236</end></extent><extent unit="pages"><start>219</start><end>228</end></extent></part></relatedItem><identifier type="istex">1642D1218149EBED06670529AD5D13DF1D549BD5</identifier><identifier type="DOI">10.1016/S0162-0134(01)00186-6</identifier><identifier type="PII">S0162-0134(01)00186-6</identifier><accessCondition type="use and reproduction" contentType="copyright">©2001 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science B.V., ©2001</recordOrigin></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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