Relationship between metallothioneins and metals in a natural population of the clam Ruditapes decussatus from Sfax coast: a non-linear model using Box-Cox transformation
Identifieur interne : 001116 ( Istex/Corpus ); précédent : 001115; suivant : 001117Relationship between metallothioneins and metals in a natural population of the clam Ruditapes decussatus from Sfax coast: a non-linear model using Box-Cox transformation
Auteurs : A. Hamza-Chaffai ; J. C. Amiard ; R. P. CossonSource :
- Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology [ 0742-8413 ] ; 1999.
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Abstract
Cadmium, copper and zinc were determined concomitantly with metallothionein-like proteins (MTLPs) in the subcellular fractions of Ruditapes decussatus digestive gland. This study covered 4 months and aimed to evaluate the effect of metal pollution and other factors such as sex, size and reproductive state on MTLP levels. Copper concentrations did not vary with month, however Cd and Zn concentrations showed high levels during August. Organisms showing low cadmium concentrations presented the highest cadmium percentages in the soluble fraction (SF) containing MTLPs. However for high cadmium concentrations, the insoluble fraction (IF) was implicated in cadmium association. MTLP levels varied according to the month, the sex and the size of the organisms. A non-linear model based on the Box-Cox transformation, was proposed to describe a positive and a significant relationship between MTLPs and the studied metals. A model including sex and size showed that these two factors affected MTLP levels, but were less important than metals. Males of R. decussatus showed higher significant correlations between MTLP levels and cadmium than females. Moreover, the effect of size and reproductive state on MTLP levels was less perceptible in males than in females. As a result, MTLPs in males of R. decussatus could be proposed as suitable biomarker for detecting metal contamination.
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DOI: 10.1016/S0742-8413(99)00023-7
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Cosson</name><affiliation><mods:affiliation>EP61-CNRS-ISOMer, 1 rue Gaston Veil, 44035 Nantes, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F4047BB902BC723D78B4A74C9C52EAA86097FDF2</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1016/S0742-8413(99)00023-7</idno><idno type="url">https://api.istex.fr/document/F4047BB902BC723D78B4A74C9C52EAA86097FDF2/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001116</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001116</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Relationship between metallothioneins and metals in a natural population of the clam Ruditapes decussatus from Sfax coast: a non-linear model using Box-Cox transformation</title><author><name sortKey="Hamza Chaffai, A" sort="Hamza Chaffai, A" uniqKey="Hamza Chaffai A" first="A." last="Hamza-Chaffai">A. Hamza-Chaffai</name><affiliation><mods:affiliation>Universite de Sfax, IPEIS, BP 805, Sfax 3018, Tunisia</mods:affiliation></affiliation></author><author><name sortKey="Amiard, J C" sort="Amiard, J C" uniqKey="Amiard J" first="J. C." last="Amiard">J. C. Amiard</name><affiliation><mods:affiliation>EP61-CNRS-ISOMer, 1 rue Gaston Veil, 44035 Nantes, France</mods:affiliation></affiliation></author><author><name sortKey="Cosson, R P" sort="Cosson, R P" uniqKey="Cosson R" first="R. P." last="Cosson">R. P. 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This study covered 4 months and aimed to evaluate the effect of metal pollution and other factors such as sex, size and reproductive state on MTLP levels. Copper concentrations did not vary with month, however Cd and Zn concentrations showed high levels during August. Organisms showing low cadmium concentrations presented the highest cadmium percentages in the soluble fraction (SF) containing MTLPs. However for high cadmium concentrations, the insoluble fraction (IF) was implicated in cadmium association. MTLP levels varied according to the month, the sex and the size of the organisms. A non-linear model based on the Box-Cox transformation, was proposed to describe a positive and a significant relationship between MTLPs and the studied metals. A model including sex and size showed that these two factors affected MTLP levels, but were less important than metals. Males of R. decussatus showed higher significant correlations between MTLP levels and cadmium than females. Moreover, the effect of size and reproductive state on MTLP levels was less perceptible in males than in females. As a result, MTLPs in males of R. decussatus could be proposed as suitable biomarker for detecting metal contamination.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>A. Hamza-Chaffai</name><affiliations><json:string>Universite de Sfax, IPEIS, BP 805, Sfax 3018, Tunisia</json:string></affiliations></json:item><json:item><name>J.C. Amiard</name><affiliations><json:string>EP61-CNRS-ISOMer, 1 rue Gaston Veil, 44035 Nantes, France</json:string></affiliations></json:item><json:item><name>R.P. Cosson</name><affiliations><json:string>EP61-CNRS-ISOMer, 1 rue Gaston Veil, 44035 Nantes, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Biomarker</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Biomonitoring</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Box-Cox transformation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Cadmium</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Copper</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Metallothionein-like proteins</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Pollution</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Ruditapes decussatus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Zinc</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Cadmium, copper and zinc were determined concomitantly with metallothionein-like proteins (MTLPs) in the subcellular fractions of Ruditapes decussatus digestive gland. This study covered 4 months and aimed to evaluate the effect of metal pollution and other factors such as sex, size and reproductive state on MTLP levels. Copper concentrations did not vary with month, however Cd and Zn concentrations showed high levels during August. Organisms showing low cadmium concentrations presented the highest cadmium percentages in the soluble fraction (SF) containing MTLPs. However for high cadmium concentrations, the insoluble fraction (IF) was implicated in cadmium association. MTLP levels varied according to the month, the sex and the size of the organisms. A non-linear model based on the Box-Cox transformation, was proposed to describe a positive and a significant relationship between MTLPs and the studied metals. A model including sex and size showed that these two factors affected MTLP levels, but were less important than metals. Males of R. decussatus showed higher significant correlations between MTLP levels and cadmium than females. Moreover, the effect of size and reproductive state on MTLP levels was less perceptible in males than in females. As a result, MTLPs in males of R. decussatus could be proposed as suitable biomarker for detecting metal contamination.</abstract><qualityIndicators><score>7.484</score><pdfVersion>1.2</pdfVersion><pdfPageSize>552 x 768 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>9</keywordCount><abstractCharCount>1383</abstractCharCount><pdfWordCount>5638</pdfWordCount><pdfCharCount>35647</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>207</abstractWordCount></qualityIndicators><title>Relationship between metallothioneins and metals in a natural population of the clam Ruditapes decussatus from Sfax coast: a non-linear model using Box-Cox transformation</title><pii><json:string>S0742-8413(99)00023-7</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>123</volume><pii><json:string>S0742-8413(00)X0039-4</json:string></pii><pages><last>163</last><first>153</first></pages><issn><json:string>0742-8413</json:string></issn><issue>2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology</title><publicationDate>1999</publicationDate></host><categories><wos><json:string>ENDOCRINOLOGY & METABOLISM</json:string><json:string>PHARMACOLOGY & PHARMACY</json:string><json:string>TOXICOLOGY</json:string><json:string>BIOCHEMISTRY & MOLECULAR BIOLOGY</json:string><json:string>ZOOLOGY</json:string></wos></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1016/S0742-8413(99)00023-7</json:string></doi><id>F4047BB902BC723D78B4A74C9C52EAA86097FDF2</id><score>0.04658293</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/F4047BB902BC723D78B4A74C9C52EAA86097FDF2/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/F4047BB902BC723D78B4A74C9C52EAA86097FDF2/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/F4047BB902BC723D78B4A74C9C52EAA86097FDF2/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Relationship between metallothioneins and metals in a natural population of the clam Ruditapes decussatus from Sfax coast: a non-linear model using Box-Cox transformation</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1999 Elsevier Science Inc.</p></availability><date>1999</date></publicationStmt><notesStmt><note type="content">Fig. 1: Month (X axis)-related variations of metal concentrations in ng/g and MTLP concentrations in mg/g (Y axis). C1F: insoluble fraction of females; C1M: insoluble fraction of males; S1F: soluble fraction of females; S1M: soluble fraction of males; M: MTLP in males, F: MTLP in females</note><note type="content">Fig. 2: Month (X axis)-related variations of metal quantities (ng) and MTLP quantities in mg (Y axis). C1F: insoluble fraction of females; C1M: insoluble fraction of males; S1F: soluble fraction of females; S1M: soluble fraction of males; M: MTLP in males, F: MTLP in females.</note><note type="content">Table 1: A result of the intercalibration exercise, mean and (SD) 13, our laboratory code is 12</note><note type="content">Table 2: MTLP, Cd, Cu and Zn: statistical significance of differences based on the montha</note><note type="content">Table 3: Metal distribution in the studied fractions with respect to total amountsa</note><note type="content">Table 4: Cadmium and zinc ratios in the different fractions with reference to the month</note><note type="content">Table 5: MTLP, Cd, Cu and Zn amounts according to the sex, statistical significancea</note><note type="content">Table 6: Results of the linear relationship between MTLPs and metalsa</note><note type="content">Table 7: Functional relationship between MTLP and metals by using both linear and non-linear models, MTLPs=f(Cd, Cu, Zn)a</note><note type="content">Table 8: Functional relationship between MTLP and the studied variables using both linear and non-linear models, MTLPs=f(Cd, Cu, Zn, Sex, Size)a</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Relationship between metallothioneins and metals in a natural population of the clam Ruditapes decussatus from Sfax coast: a non-linear model using Box-Cox transformation</title><author xml:id="author-1"><persName><forename type="first">A.</forename><surname>Hamza-Chaffai</surname></persName><note type="correspondence"><p>Corresponding author. Tel.: +216-4-614109; fax: +216-4-614109</p></note><affiliation>Universite de Sfax, IPEIS, BP 805, Sfax 3018, Tunisia</affiliation></author><author xml:id="author-2"><persName><forename type="first">J.C.</forename><surname>Amiard</surname></persName><affiliation>EP61-CNRS-ISOMer, 1 rue Gaston Veil, 44035 Nantes, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">R.P.</forename><surname>Cosson</surname></persName><affiliation>EP61-CNRS-ISOMer, 1 rue Gaston Veil, 44035 Nantes, France</affiliation></author></analytic><monogr><title level="j">Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology</title><title level="j" type="abbrev">CBCOLD</title><idno type="pISSN">0742-8413</idno><idno type="PII">S0742-8413(00)X0039-4</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">123</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="153">153</biblScope><biblScope unit="page" to="163">163</biblScope></imprint></monogr><idno type="istex">F4047BB902BC723D78B4A74C9C52EAA86097FDF2</idno><idno type="DOI">10.1016/S0742-8413(99)00023-7</idno><idno type="PII">S0742-8413(99)00023-7</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Cadmium, copper and zinc were determined concomitantly with metallothionein-like proteins (MTLPs) in the subcellular fractions of Ruditapes decussatus digestive gland. This study covered 4 months and aimed to evaluate the effect of metal pollution and other factors such as sex, size and reproductive state on MTLP levels. Copper concentrations did not vary with month, however Cd and Zn concentrations showed high levels during August. Organisms showing low cadmium concentrations presented the highest cadmium percentages in the soluble fraction (SF) containing MTLPs. However for high cadmium concentrations, the insoluble fraction (IF) was implicated in cadmium association. MTLP levels varied according to the month, the sex and the size of the organisms. A non-linear model based on the Box-Cox transformation, was proposed to describe a positive and a significant relationship between MTLPs and the studied metals. A model including sex and size showed that these two factors affected MTLP levels, but were less important than metals. Males of R. decussatus showed higher significant correlations between MTLP levels and cadmium than females. Moreover, the effect of size and reproductive state on MTLP levels was less perceptible in males than in females. As a result, MTLPs in males of R. decussatus could be proposed as suitable biomarker for detecting metal contamination.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Biomarker</term></item><item><term>Biomonitoring</term></item><item><term>Box-Cox transformation</term></item><item><term>Cadmium</term></item><item><term>Copper</term></item><item><term>Metallothionein-like proteins</term></item><item><term>Pollution</term></item><item><term>Ruditapes decussatus</term></item><item><term>Zinc</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999-02-23">Modified</change><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/F4047BB902BC723D78B4A74C9C52EAA86097FDF2/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>CBC</jid><aid>6150</aid><ce:pii>S0742-8413(99)00023-7</ce:pii><ce:doi>10.1016/S0742-8413(99)00023-7</ce:doi><ce:copyright type="full-transfer" year="1999">Elsevier Science Inc.</ce:copyright></item-info><head><ce:title>Relationship between metallothioneins and metals in a natural population of the clam <ce:italic>Ruditapes decussatus</ce:italic> from Sfax coast: a non-linear model using Box-Cox transformation</ce:title><ce:author-group><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Hamza-Chaffai</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:author><ce:given-name>J.C.</ce:given-name><ce:surname>Amiard</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>R.P.</ce:given-name><ce:surname>Cosson</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Universite de Sfax, IPEIS, BP 805, Sfax 3018, Tunisia</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>EP61-CNRS-ISOMer, 1 rue Gaston Veil, 44035 Nantes, France</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +216-4-614109; fax: +216-4-614109</ce:text></ce:correspondence></ce:author-group><ce:date-received day="15" month="5" year="1998"></ce:date-received><ce:date-revised day="23" month="2" year="1999"></ce:date-revised><ce:date-accepted day="3" month="3" year="1999"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Cadmium, copper and zinc were determined concomitantly with metallothionein-like proteins (MTLPs) in the subcellular fractions of <ce:italic>Ruditapes decussatus</ce:italic> digestive gland. This study covered 4 months and aimed to evaluate the effect of metal pollution and other factors such as sex, size and reproductive state on MTLP levels. Copper concentrations did not vary with month, however Cd and Zn concentrations showed high levels during August. Organisms showing low cadmium concentrations presented the highest cadmium percentages in the soluble fraction (SF) containing MTLPs. However for high cadmium concentrations, the insoluble fraction (IF) was implicated in cadmium association. MTLP levels varied according to the month, the sex and the size of the organisms. A non-linear model based on the Box-Cox transformation, was proposed to describe a positive and a significant relationship between MTLPs and the studied metals. A model including sex and size showed that these two factors affected MTLP levels, but were less important than metals. Males of <ce:italic>R. decussatus</ce:italic> showed higher significant correlations between MTLP levels and cadmium than females. Moreover, the effect of size and reproductive state on MTLP levels was less perceptible in males than in females. As a result, MTLPs in males of <ce:italic>R. decussatus</ce:italic> could be proposed as suitable biomarker for detecting metal contamination.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Biomarker</ce:text></ce:keyword><ce:keyword><ce:text>Biomonitoring</ce:text></ce:keyword><ce:keyword><ce:text>Box-Cox transformation</ce:text></ce:keyword><ce:keyword><ce:text>Cadmium</ce:text></ce:keyword><ce:keyword><ce:text>Copper</ce:text></ce:keyword><ce:keyword><ce:text>Metallothionein-like proteins</ce:text></ce:keyword><ce:keyword><ce:text>Pollution</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>Ruditapes decussatus</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Zinc</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Relationship between metallothioneins and metals in a natural population of the clam Ruditapes decussatus from Sfax coast: a non-linear model using Box-Cox transformation</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Relationship between metallothioneins and metals in a natural population of the clam</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Hamza-Chaffai</namePart><affiliation>Universite de Sfax, IPEIS, BP 805, Sfax 3018, Tunisia</affiliation><description>Corresponding author. Tel.: +216-4-614109; fax: +216-4-614109</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.C.</namePart><namePart type="family">Amiard</namePart><affiliation>EP61-CNRS-ISOMer, 1 rue Gaston Veil, 44035 Nantes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.P.</namePart><namePart type="family">Cosson</namePart><affiliation>EP61-CNRS-ISOMer, 1 rue Gaston Veil, 44035 Nantes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><dateModified encoding="w3cdtf">1999-02-23</dateModified><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Cadmium, copper and zinc were determined concomitantly with metallothionein-like proteins (MTLPs) in the subcellular fractions of Ruditapes decussatus digestive gland. This study covered 4 months and aimed to evaluate the effect of metal pollution and other factors such as sex, size and reproductive state on MTLP levels. Copper concentrations did not vary with month, however Cd and Zn concentrations showed high levels during August. Organisms showing low cadmium concentrations presented the highest cadmium percentages in the soluble fraction (SF) containing MTLPs. However for high cadmium concentrations, the insoluble fraction (IF) was implicated in cadmium association. MTLP levels varied according to the month, the sex and the size of the organisms. A non-linear model based on the Box-Cox transformation, was proposed to describe a positive and a significant relationship between MTLPs and the studied metals. A model including sex and size showed that these two factors affected MTLP levels, but were less important than metals. Males of R. decussatus showed higher significant correlations between MTLP levels and cadmium than females. Moreover, the effect of size and reproductive state on MTLP levels was less perceptible in males than in females. As a result, MTLPs in males of R. decussatus could be proposed as suitable biomarker for detecting metal contamination.</abstract><note type="content">Fig. 1: Month (X axis)-related variations of metal concentrations in ng/g and MTLP concentrations in mg/g (Y axis). C1F: insoluble fraction of females; C1M: insoluble fraction of males; S1F: soluble fraction of females; S1M: soluble fraction of males; M: MTLP in males, F: MTLP in females</note><note type="content">Fig. 2: Month (X axis)-related variations of metal quantities (ng) and MTLP quantities in mg (Y axis). C1F: insoluble fraction of females; C1M: insoluble fraction of males; S1F: soluble fraction of females; S1M: soluble fraction of males; M: MTLP in males, F: MTLP in females.</note><note type="content">Table 1: A result of the intercalibration exercise, mean and (SD) 13, our laboratory code is 12</note><note type="content">Table 2: MTLP, Cd, Cu and Zn: statistical significance of differences based on the montha</note><note type="content">Table 3: Metal distribution in the studied fractions with respect to total amountsa</note><note type="content">Table 4: Cadmium and zinc ratios in the different fractions with reference to the month</note><note type="content">Table 5: MTLP, Cd, Cu and Zn amounts according to the sex, statistical significancea</note><note type="content">Table 6: Results of the linear relationship between MTLPs and metalsa</note><note type="content">Table 7: Functional relationship between MTLP and metals by using both linear and non-linear models, MTLPs=f(Cd, Cu, Zn)a</note><note type="content">Table 8: Functional relationship between MTLP and the studied variables using both linear and non-linear models, MTLPs=f(Cd, Cu, Zn, Sex, Size)a</note><subject lang="en"><genre>Keywords</genre><topic>Biomarker</topic><topic>Biomonitoring</topic><topic>Box-Cox transformation</topic><topic>Cadmium</topic><topic>Copper</topic><topic>Metallothionein-like proteins</topic><topic>Pollution</topic><topic>Ruditapes decussatus</topic><topic>Zinc</topic></subject><relatedItem type="host"><titleInfo><title>Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology</title></titleInfo><titleInfo type="abbreviated"><title>CBCOLD</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199906</dateIssued></originInfo><identifier type="ISSN">0742-8413</identifier><identifier type="PII">S0742-8413(00)X0039-4</identifier><part><date>199906</date><detail type="volume"><number>123</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue pages"><start>103</start><end>192</end></extent><extent unit="pages"><start>153</start><end>163</end></extent></part></relatedItem><identifier type="istex">F4047BB902BC723D78B4A74C9C52EAA86097FDF2</identifier><identifier type="DOI">10.1016/S0742-8413(99)00023-7</identifier><identifier type="PII">S0742-8413(99)00023-7</identifier><accessCondition type="use and reproduction" contentType="copyright">©1999 Elsevier Science Inc.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Inc., ©1999</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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