Coping with cadmium exposure in various ways: the two Helicid snails Helix pomatia and Cantareus aspersus share the metal transcription factor‐2, but differ in promoter organization and transcription of their Cd‐metallothionein genes
Identifieur interne : 000071 ( Main/Exploration ); précédent : 000070; suivant : 000072Coping with cadmium exposure in various ways: the two Helicid snails Helix pomatia and Cantareus aspersus share the metal transcription factor‐2, but differ in promoter organization and transcription of their Cd‐metallothionein genes
Auteurs : M. Höckner [Autriche] ; K. Stefanon [Autriche] ; D. Schuler [Autriche] ; R. Fantur [Autriche] ; A. de Vaufleury [France] ; R. Dallinger [Autriche]Source :
- Journal of Experimental Zoology Part A: Ecological Genetics and Physiology [ 1932-5223 ] ; 2009-12-01.
Abstract
Gastropods are able to withstand fluctuating availabilities of nonessential trace elements such as cadmium by induction of Cd‐specific metallothionein isoform (Cd‐MT) expression. As in other species, the induction mechanism involves the binding of metal‐regulatory transcription factors (MTF‐1 or MTF‐2) to metal responsive elements (MREs) in the MT promoter regions. Cd‐dependent transcription of Cd‐MT genes was assessed by quantitative real time PCR in two helicid gastropods, Helix pomatia and Cantareus aspersus, over a period of eight days. The promoter regions of the Cd‐MT genes of the two species were sequenced and compared regarding the position of MREs and other relevant potential transcription factor binding sites (TFBs). Cd‐MT gene transcription is induced after Cd exposure in Helix pomatia and Cantareus aspersus, showing a transient peak in Helix pomatia, contrasting with a persistent induction rate in Cantareus aspersus. Since the existence of MTF‐2 was verified in both species, differing transcription patterns of Cd‐MT genes must be due to functional differences in their metal‐responsive promoter regions. Both promoters contain a proximal cluster of three MREs overlapping with TFBs for the transcriptional regulator Sp1. In contrast to Cantareus aspersus, however, the Cd‐MT gene of Helix pomatia hosts an additional distal MRE overlapping with a Sp1 binding site and a CACCC box. Inhibitory effects of MRE overlapping Sp1 binding sites were observed in other MT genes. We therefore suggest that transient Cd‐MT transcription upon Cd2+ exposure in Helix pomatia may be the result of an inhibitory action of the distal MRE cluster. J. Exp. Zool. 311A:776–787, 2009. © 2009 Wiley‐Liss, Inc.
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DOI: 10.1002/jez.564
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Zool.</title><idno type="ISSN">1932-5223</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2009-12-01">2009-12-01</date><biblScope unit="vol">311A</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="776">776</biblScope><biblScope unit="page" to="787">787</biblScope></imprint><idno type="ISSN">1932-5223</idno></series><idno type="istex">A0BE09B57102718759CA06C38A2993D732B1B498</idno><idno type="DOI">10.1002/jez.564</idno><idno type="ArticleID">JEZ564</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1932-5223</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Gastropods are able to withstand fluctuating availabilities of nonessential trace elements such as cadmium by induction of Cd‐specific metallothionein isoform (Cd‐MT) expression. As in other species, the induction mechanism involves the binding of metal‐regulatory transcription factors (MTF‐1 or MTF‐2) to metal responsive elements (MREs) in the MT promoter regions. Cd‐dependent transcription of Cd‐MT genes was assessed by quantitative real time PCR in two helicid gastropods, Helix pomatia and Cantareus aspersus, over a period of eight days. The promoter regions of the Cd‐MT genes of the two species were sequenced and compared regarding the position of MREs and other relevant potential transcription factor binding sites (TFBs). Cd‐MT gene transcription is induced after Cd exposure in Helix pomatia and Cantareus aspersus, showing a transient peak in Helix pomatia, contrasting with a persistent induction rate in Cantareus aspersus. Since the existence of MTF‐2 was verified in both species, differing transcription patterns of Cd‐MT genes must be due to functional differences in their metal‐responsive promoter regions. Both promoters contain a proximal cluster of three MREs overlapping with TFBs for the transcriptional regulator Sp1. In contrast to Cantareus aspersus, however, the Cd‐MT gene of Helix pomatia hosts an additional distal MRE overlapping with a Sp1 binding site and a CACCC box. Inhibitory effects of MRE overlapping Sp1 binding sites were observed in other MT genes. We therefore suggest that transient Cd‐MT transcription upon Cd2+ exposure in Helix pomatia may be the result of an inhibitory action of the distal MRE cluster. J. Exp. Zool. 311A:776–787, 2009. © 2009 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Autriche</li><li>France</li></country></list><tree><country name="Autriche"><noRegion><name sortKey="H Ckner, M" sort="H Ckner, M" uniqKey="H Ckner M" first="M." last="Höckner">M. Höckner</name></noRegion><name sortKey="Dallinger, R" sort="Dallinger, R" uniqKey="Dallinger R" first="R." last="Dallinger">R. Dallinger</name><name sortKey="Fantur, R" sort="Fantur, R" uniqKey="Fantur R" first="R." last="Fantur">R. Fantur</name><name sortKey="Schuler, D" sort="Schuler, D" uniqKey="Schuler D" first="D." last="Schuler">D. Schuler</name><name sortKey="Stefanon, K" sort="Stefanon, K" uniqKey="Stefanon K" first="K." last="Stefanon">K. Stefanon</name></country><country name="France"><noRegion><name sortKey="De Vaufleury, A" sort="De Vaufleury, A" uniqKey="De Vaufleury A" first="A." last="de Vaufleury">A. de Vaufleury</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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