Fluorescence microscopy imaging of bone for automated histomorphometry.
Identifieur interne : 000615 ( PubMed/Checkpoint ); précédent : 000614; suivant : 000616Fluorescence microscopy imaging of bone for automated histomorphometry.
Auteurs : Ivan Martin [Italie] ; Maddalena Mastrogiacomo ; Gianluca De Leo ; Anita Muraglia ; Francesco Beltrame ; Ranieri Cancedda ; Rodolfo QuartoSource :
- Tissue engineering [ 1076-3279 ] ; 2002.
Descripteurs français
- KwdFr :
- MESH :
- cytologie : Cellules de la moelle osseuse, Os et tissu osseux.
- usage thérapeutique : Durapatite.
- Humains, Ingénierie tissulaire, Microscopie de fluorescence, Transplantation de moelle osseuse.
English descriptors
- KwdEn :
- MESH :
- chemical , therapeutic use : Durapatite.
- cytology : Bone Marrow Cells, Bone and Bones.
- Bone Marrow Transplantation, Humans, Microscopy, Fluorescence, Tissue Engineering.
Abstract
We have developed a computer-based method for the automated quantification of bone tissue in histological sections of decalcified specimens. Bone tissue was generated by ectopic implantation of ceramic-based carriers loaded with human bone marrow stromal cells (BMSCs). The method is based on the acquisition of multimodal images, in order to identify and measure the area covered by bone tissue (using fluorescent light) and the total area of tissue (using transmitted light), thereby excluding the regions corresponding to nonresorbed scaffold. The amount of bone as a percentage of the total area of interest (bone/area) and of the newly formed tissue (bone/tissue) is automatically derived. The computer-based results correlated closely with those obtained by manual identification of bone and tissue areas in the same histological fields (R(2) = 0.997; p < 0.0005), with errors dependent on the magnification used but always lower than 9.4%. The method was used to compare the bone/tissue and bone/area percentages in samples of engineered bone based on human BMSCs expanded in the presence of different biochemical factors and loaded onto different scaffolds. The technique thus represents a valuable tool to quantify reproducibly, accurately, and easily bone formation in a variety of tissue-engineering studies.
DOI: 10.1089/10763270260424204
PubMed: 12459063
Affiliations:
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first="Gianluca" last="De Leo">Gianluca De Leo</name></author><author><name sortKey="Muraglia, Anita" sort="Muraglia, Anita" uniqKey="Muraglia A" first="Anita" last="Muraglia">Anita Muraglia</name></author><author><name sortKey="Beltrame, Francesco" sort="Beltrame, Francesco" uniqKey="Beltrame F" first="Francesco" last="Beltrame">Francesco Beltrame</name></author><author><name sortKey="Cancedda, Ranieri" sort="Cancedda, Ranieri" uniqKey="Cancedda R" first="Ranieri" last="Cancedda">Ranieri Cancedda</name></author><author><name sortKey="Quarto, Rodolfo" sort="Quarto, Rodolfo" uniqKey="Quarto R" first="Rodolfo" last="Quarto">Rodolfo Quarto</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12459063</idno><idno type="pmid">12459063</idno><idno type="doi">10.1089/10763270260424204</idno><idno type="wicri:Area/PubMed/Corpus">000635</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" 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Genoa</wicri:regionArea><wicri:noRegion>16132 Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Mastrogiacomo, Maddalena" sort="Mastrogiacomo, Maddalena" uniqKey="Mastrogiacomo M" first="Maddalena" last="Mastrogiacomo">Maddalena Mastrogiacomo</name></author><author><name sortKey="De Leo, Gianluca" sort="De Leo, Gianluca" uniqKey="De Leo G" first="Gianluca" last="De Leo">Gianluca De Leo</name></author><author><name sortKey="Muraglia, Anita" sort="Muraglia, Anita" uniqKey="Muraglia A" first="Anita" last="Muraglia">Anita Muraglia</name></author><author><name sortKey="Beltrame, Francesco" sort="Beltrame, Francesco" uniqKey="Beltrame F" first="Francesco" last="Beltrame">Francesco Beltrame</name></author><author><name sortKey="Cancedda, Ranieri" sort="Cancedda, Ranieri" uniqKey="Cancedda R" first="Ranieri" last="Cancedda">Ranieri Cancedda</name></author><author><name sortKey="Quarto, Rodolfo" sort="Quarto, Rodolfo" uniqKey="Quarto R" first="Rodolfo" last="Quarto">Rodolfo Quarto</name></author></analytic><series><title level="j">Tissue engineering</title><idno type="ISSN">1076-3279</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bone Marrow Cells (cytology)</term><term>Bone Marrow Transplantation</term><term>Bone and Bones (cytology)</term><term>Durapatite (therapeutic use)</term><term>Humans</term><term>Microscopy, Fluorescence</term><term>Tissue Engineering</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules de la moelle osseuse (cytologie)</term><term>Durapatite (usage thérapeutique)</term><term>Humains</term><term>Ingénierie tissulaire</term><term>Microscopie de fluorescence</term><term>Os et tissu osseux (cytologie)</term><term>Transplantation de moelle osseuse</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Durapatite</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules de la moelle osseuse</term><term>Os et tissu osseux</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Bone Marrow Cells</term><term>Bone and Bones</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Durapatite</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bone Marrow Transplantation</term><term>Humans</term><term>Microscopy, Fluorescence</term><term>Tissue Engineering</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Ingénierie tissulaire</term><term>Microscopie de fluorescence</term><term>Transplantation de moelle osseuse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have developed a computer-based method for the automated quantification of bone tissue in histological sections of decalcified specimens. Bone tissue was generated by ectopic implantation of ceramic-based carriers loaded with human bone marrow stromal cells (BMSCs). The method is based on the acquisition of multimodal images, in order to identify and measure the area covered by bone tissue (using fluorescent light) and the total area of tissue (using transmitted light), thereby excluding the regions corresponding to nonresorbed scaffold. The amount of bone as a percentage of the total area of interest (bone/area) and of the newly formed tissue (bone/tissue) is automatically derived. The computer-based results correlated closely with those obtained by manual identification of bone and tissue areas in the same histological fields (R(2) = 0.997; p < 0.0005), with errors dependent on the magnification used but always lower than 9.4%. The method was used to compare the bone/tissue and bone/area percentages in samples of engineered bone based on human BMSCs expanded in the presence of different biochemical factors and loaded onto different scaffolds. The technique thus represents a valuable tool to quantify reproducibly, accurately, and easily bone formation in a variety of tissue-engineering studies.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">12459063</PMID><DateCreated><Year>2002</Year><Month>12</Month><Day>02</Day></DateCreated><DateCompleted><Year>2003</Year><Month>05</Month><Day>01</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1076-3279</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>5</Issue><PubDate><Year>2002</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Tissue engineering</Title><ISOAbbreviation>Tissue Eng.</ISOAbbreviation></Journal><ArticleTitle>Fluorescence microscopy imaging of bone for automated histomorphometry.</ArticleTitle><Pagination><MedlinePgn>847-52</MedlinePgn></Pagination><Abstract><AbstractText>We have developed a computer-based method for the automated quantification of bone tissue in histological sections of decalcified specimens. Bone tissue was generated by ectopic implantation of ceramic-based carriers loaded with human bone marrow stromal cells (BMSCs). The method is based on the acquisition of multimodal images, in order to identify and measure the area covered by bone tissue (using fluorescent light) and the total area of tissue (using transmitted light), thereby excluding the regions corresponding to nonresorbed scaffold. The amount of bone as a percentage of the total area of interest (bone/area) and of the newly formed tissue (bone/tissue) is automatically derived. The computer-based results correlated closely with those obtained by manual identification of bone and tissue areas in the same histological fields (R(2) = 0.997; p < 0.0005), with errors dependent on the magnification used but always lower than 9.4%. The method was used to compare the bone/tissue and bone/area percentages in samples of engineered bone based on human BMSCs expanded in the presence of different biochemical factors and loaded onto different scaffolds. The technique thus represents a valuable tool to quantify reproducibly, accurately, and easily bone formation in a variety of tissue-engineering studies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Martin</LastName><ForeName>Ivan</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Dipartimento di Informatica, Sistemistica e Telematica (DIST), Università di Genova, Largo Rosanna Benzi n. 10, 16132 Genoa, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mastrogiacomo</LastName><ForeName>Maddalena</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>De Leo</LastName><ForeName>Gianluca</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Muraglia</LastName><ForeName>Anita</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Beltrame</LastName><ForeName>Francesco</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Cancedda</LastName><ForeName>Ranieri</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Quarto</LastName><ForeName>Rodolfo</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Tissue Eng</MedlineTA><NlmUniqueID>9505538</NlmUniqueID><ISSNLinking>1076-3279</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>91D9GV0Z28</RegistryNumber><NameOfSubstance UI="D017886">Durapatite</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D001854">Bone Marrow Cells</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000166">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D016026">Bone Marrow Transplantation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D001842">Bone and Bones</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000166">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017886">Durapatite</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000627">therapeutic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006801">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008856">Microscopy, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D023822">Tissue Engineering</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>12</Month><Day>3</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>5</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>12</Month><Day>3</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12459063</ArticleId><ArticleId IdType="doi">10.1089/10763270260424204</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Beltrame, Francesco" sort="Beltrame, Francesco" uniqKey="Beltrame F" first="Francesco" last="Beltrame">Francesco Beltrame</name><name sortKey="Cancedda, Ranieri" sort="Cancedda, Ranieri" uniqKey="Cancedda R" first="Ranieri" last="Cancedda">Ranieri Cancedda</name><name sortKey="De Leo, Gianluca" sort="De Leo, Gianluca" uniqKey="De Leo G" first="Gianluca" last="De Leo">Gianluca De Leo</name><name sortKey="Mastrogiacomo, Maddalena" sort="Mastrogiacomo, Maddalena" uniqKey="Mastrogiacomo M" first="Maddalena" last="Mastrogiacomo">Maddalena Mastrogiacomo</name><name sortKey="Muraglia, Anita" sort="Muraglia, Anita" uniqKey="Muraglia A" first="Anita" last="Muraglia">Anita Muraglia</name><name sortKey="Quarto, Rodolfo" sort="Quarto, Rodolfo" uniqKey="Quarto R" first="Rodolfo" last="Quarto">Rodolfo Quarto</name></noCountry><country name="Italie"><noRegion><name sortKey="Martin, Ivan" sort="Martin, Ivan" uniqKey="Martin I" first="Ivan" last="Martin">Ivan Martin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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