Comparison of the three optical platforms for measurement of cellular respiration.
Identifieur interne : 000012 ( PubMed/Curation ); précédent : 000011; suivant : 000013Comparison of the three optical platforms for measurement of cellular respiration.
Auteurs : Alina V. Kondrashina [Irlande (pays)] ; Vladimir I. Ogurtsov [Irlande (pays)] ; Dmitri B. Papkovsky [Irlande (pays)]Source :
- Analytical biochemistry [ 1096-0309 ] ; 2014.
Abstract
We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.
DOI: 10.1016/j.ab.2014.09.001
PubMed: 25233002
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<front><div type="abstract" xml:lang="en">We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.</div>
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