Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections.
Identifieur interne : 000002 ( PubMed/Corpus ); précédent : 000001; suivant : 000003Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections.
Auteurs : Timothée Houssin ; Jérémy Cramer ; Rébecca Grojsman ; Lyes Bellahsene ; Guillaume Colas ; Hélène Moulet ; Walter Minnella ; Christophe Pannetier ; Maël Leberre ; Adrien Plecis ; Yong ChenSource :
- Lab on a chip [ 1473-0189 ] ; 2016.
Abstract
To control future infectious disease outbreaks, like the 2014 Ebola epidemic, it is necessary to develop ultrafast molecular assays enabling rapid and sensitive diagnoses. To that end, several ultrafast real-time PCR systems have been previously developed, but they present issues that hinder their wide adoption, notably regarding their sensitivity and detection volume. An ultrafast, sensitive and large-volume real-time PCR system based on microfluidic thermalization is presented herein. The method is based on the circulation of pre-heated liquids in a microfluidic chip that thermalize the PCR chamber by diffusion and ultrafast flow switches. The system can achieve up to 30 real-time PCR cycles in around 2 minutes, which makes it the fastest PCR thermalization system for regular sample volume to the best of our knowledge. After biochemical optimization, anthrax and Ebola simulating agents could be respectively detected by a real-time PCR in 7 minutes and a reverse transcription real-time PCR in 7.5 minutes. These detections are respectively 6.4 and 7.2 times faster than with an off-the-shelf apparatus, while conserving real-time PCR sample volume, efficiency, selectivity and sensitivity. The high-speed thermalization also enabled us to perform sharp melting curve analyses in only 20 s and to discriminate amplicons of different lengths by rapid real-time PCR. This real-time PCR microfluidic thermalization system is cost-effective, versatile and can be then further developed for point-of-care, multiplexed, ultrafast and highly sensitive molecular diagnoses of bacterial and viral diseases.
DOI: 10.1039/c5lc01459j
PubMed: 26952334
Links to Exploration step
pubmed:26952334Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections.</title><author><name sortKey="Houssin, Timothee" sort="Houssin, Timothee" uniqKey="Houssin T" first="Timothée" last="Houssin">Timothée Houssin</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com and École Normale Supérieure-PSL Research University, 24 rue Lhomond, 75005, Paris, France. yong.chen@ens.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Cramer, Jeremy" sort="Cramer, Jeremy" uniqKey="Cramer J" first="Jérémy" last="Cramer">Jérémy Cramer</name><affiliation><nlm:affiliation>École Normale Supérieure-PSL Research University, 24 rue Lhomond, 75005, Paris, France. yong.chen@ens.fr and Cherry Biotech, Université de Rennes 1, Campus Santé, Bât8, Et2, 2 Av. du Pr. Léon Bernard, CS 34317, 35043 Rennes Cedex, France. jeremy.cramer@cherrybiotech.com.</nlm:affiliation></affiliation></author><author><name sortKey="Grojsman, Rebecca" sort="Grojsman, Rebecca" uniqKey="Grojsman R" first="Rébecca" last="Grojsman">Rébecca Grojsman</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Bellahsene, Lyes" sort="Bellahsene, Lyes" uniqKey="Bellahsene L" first="Lyes" last="Bellahsene">Lyes Bellahsene</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Colas, Guillaume" sort="Colas, Guillaume" uniqKey="Colas G" first="Guillaume" last="Colas">Guillaume Colas</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Moulet, Helene" sort="Moulet, Helene" uniqKey="Moulet H" first="Hélène" last="Moulet">Hélène Moulet</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Minnella, Walter" sort="Minnella, Walter" uniqKey="Minnella W" first="Walter" last="Minnella">Walter Minnella</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Pannetier, Christophe" sort="Pannetier, Christophe" uniqKey="Pannetier C" first="Christophe" last="Pannetier">Christophe Pannetier</name><affiliation><nlm:affiliation>Episteme Research and Consulting, 1 rue Rabelais, 92170, Vanves, France. christophe.pannetier@epistemerc.eu.</nlm:affiliation></affiliation></author><author><name sortKey="Leberre, Mael" sort="Leberre, Mael" uniqKey="Leberre M" first="Maël" last="Leberre">Maël Leberre</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Plecis, Adrien" sort="Plecis, Adrien" uniqKey="Plecis A" first="Adrien" last="Plecis">Adrien Plecis</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Yong" sort="Chen, Yong" uniqKey="Chen Y" first="Yong" last="Chen">Yong Chen</name><affiliation><nlm:affiliation>École Normale Supérieure-PSL Research University, 24 rue Lhomond, 75005, Paris, France. yong.chen@ens.fr.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="doi">10.1039/c5lc01459j</idno><idno type="RBID">pubmed:26952334</idno><idno type="pmid">26952334</idno><idno type="wicri:Area/PubMed/Corpus">000002</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000002</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections.</title><author><name sortKey="Houssin, Timothee" sort="Houssin, Timothee" uniqKey="Houssin T" first="Timothée" last="Houssin">Timothée Houssin</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com and École Normale Supérieure-PSL Research University, 24 rue Lhomond, 75005, Paris, France. yong.chen@ens.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Cramer, Jeremy" sort="Cramer, Jeremy" uniqKey="Cramer J" first="Jérémy" last="Cramer">Jérémy Cramer</name><affiliation><nlm:affiliation>École Normale Supérieure-PSL Research University, 24 rue Lhomond, 75005, Paris, France. yong.chen@ens.fr and Cherry Biotech, Université de Rennes 1, Campus Santé, Bât8, Et2, 2 Av. du Pr. Léon Bernard, CS 34317, 35043 Rennes Cedex, France. jeremy.cramer@cherrybiotech.com.</nlm:affiliation></affiliation></author><author><name sortKey="Grojsman, Rebecca" sort="Grojsman, Rebecca" uniqKey="Grojsman R" first="Rébecca" last="Grojsman">Rébecca Grojsman</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Bellahsene, Lyes" sort="Bellahsene, Lyes" uniqKey="Bellahsene L" first="Lyes" last="Bellahsene">Lyes Bellahsene</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Colas, Guillaume" sort="Colas, Guillaume" uniqKey="Colas G" first="Guillaume" last="Colas">Guillaume Colas</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Moulet, Helene" sort="Moulet, Helene" uniqKey="Moulet H" first="Hélène" last="Moulet">Hélène Moulet</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Minnella, Walter" sort="Minnella, Walter" uniqKey="Minnella W" first="Walter" last="Minnella">Walter Minnella</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Pannetier, Christophe" sort="Pannetier, Christophe" uniqKey="Pannetier C" first="Christophe" last="Pannetier">Christophe Pannetier</name><affiliation><nlm:affiliation>Episteme Research and Consulting, 1 rue Rabelais, 92170, Vanves, France. christophe.pannetier@epistemerc.eu.</nlm:affiliation></affiliation></author><author><name sortKey="Leberre, Mael" sort="Leberre, Mael" uniqKey="Leberre M" first="Maël" last="Leberre">Maël Leberre</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Plecis, Adrien" sort="Plecis, Adrien" uniqKey="Plecis A" first="Adrien" last="Plecis">Adrien Plecis</name><affiliation><nlm:affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Yong" sort="Chen, Yong" uniqKey="Chen Y" first="Yong" last="Chen">Yong Chen</name><affiliation><nlm:affiliation>École Normale Supérieure-PSL Research University, 24 rue Lhomond, 75005, Paris, France. yong.chen@ens.fr.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Lab on a chip</title><idno type="eISSN">1473-0189</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To control future infectious disease outbreaks, like the 2014 Ebola epidemic, it is necessary to develop ultrafast molecular assays enabling rapid and sensitive diagnoses. To that end, several ultrafast real-time PCR systems have been previously developed, but they present issues that hinder their wide adoption, notably regarding their sensitivity and detection volume. An ultrafast, sensitive and large-volume real-time PCR system based on microfluidic thermalization is presented herein. The method is based on the circulation of pre-heated liquids in a microfluidic chip that thermalize the PCR chamber by diffusion and ultrafast flow switches. The system can achieve up to 30 real-time PCR cycles in around 2 minutes, which makes it the fastest PCR thermalization system for regular sample volume to the best of our knowledge. After biochemical optimization, anthrax and Ebola simulating agents could be respectively detected by a real-time PCR in 7 minutes and a reverse transcription real-time PCR in 7.5 minutes. These detections are respectively 6.4 and 7.2 times faster than with an off-the-shelf apparatus, while conserving real-time PCR sample volume, efficiency, selectivity and sensitivity. The high-speed thermalization also enabled us to perform sharp melting curve analyses in only 20 s and to discriminate amplicons of different lengths by rapid real-time PCR. This real-time PCR microfluidic thermalization system is cost-effective, versatile and can be then further developed for point-of-care, multiplexed, ultrafast and highly sensitive molecular diagnoses of bacterial and viral diseases.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">26952334</PMID><DateCreated><Year>2016</Year><Month>04</Month><Day>12</Day></DateCreated><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1473-0189</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><Issue>8</Issue><PubDate><Year>2016</Year><Month>Apr</Month><Day>21</Day></PubDate></JournalIssue><Title>Lab on a chip</Title><ISOAbbreviation>Lab Chip</ISOAbbreviation></Journal><ArticleTitle>Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections.</ArticleTitle><Pagination><MedlinePgn>1401-11</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c5lc01459j</ELocationID><Abstract><AbstractText>To control future infectious disease outbreaks, like the 2014 Ebola epidemic, it is necessary to develop ultrafast molecular assays enabling rapid and sensitive diagnoses. To that end, several ultrafast real-time PCR systems have been previously developed, but they present issues that hinder their wide adoption, notably regarding their sensitivity and detection volume. An ultrafast, sensitive and large-volume real-time PCR system based on microfluidic thermalization is presented herein. The method is based on the circulation of pre-heated liquids in a microfluidic chip that thermalize the PCR chamber by diffusion and ultrafast flow switches. The system can achieve up to 30 real-time PCR cycles in around 2 minutes, which makes it the fastest PCR thermalization system for regular sample volume to the best of our knowledge. After biochemical optimization, anthrax and Ebola simulating agents could be respectively detected by a real-time PCR in 7 minutes and a reverse transcription real-time PCR in 7.5 minutes. These detections are respectively 6.4 and 7.2 times faster than with an off-the-shelf apparatus, while conserving real-time PCR sample volume, efficiency, selectivity and sensitivity. The high-speed thermalization also enabled us to perform sharp melting curve analyses in only 20 s and to discriminate amplicons of different lengths by rapid real-time PCR. This real-time PCR microfluidic thermalization system is cost-effective, versatile and can be then further developed for point-of-care, multiplexed, ultrafast and highly sensitive molecular diagnoses of bacterial and viral diseases.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Houssin</LastName><ForeName>Timothée</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com and École Normale Supérieure-PSL Research University, 24 rue Lhomond, 75005, Paris, France. yong.chen@ens.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cramer</LastName><ForeName>Jérémy</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>École Normale Supérieure-PSL Research University, 24 rue Lhomond, 75005, Paris, France. yong.chen@ens.fr and Cherry Biotech, Université de Rennes 1, Campus Santé, Bât8, Et2, 2 Av. du Pr. Léon Bernard, CS 34317, 35043 Rennes Cedex, France. jeremy.cramer@cherrybiotech.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grojsman</LastName><ForeName>Rébecca</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bellahsene</LastName><ForeName>Lyes</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Colas</LastName><ForeName>Guillaume</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moulet</LastName><ForeName>Hélène</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Minnella</LastName><ForeName>Walter</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pannetier</LastName><ForeName>Christophe</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Episteme Research and Consulting, 1 rue Rabelais, 92170, Vanves, France. christophe.pannetier@epistemerc.eu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leberre</LastName><ForeName>Maël</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Plecis</LastName><ForeName>Adrien</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Elvesys - Innovation Center, 83 avenue Philippe Auguste, 75011, Paris, France. houssintimothee@gmail.com mael.leberre@elvesys.com adrien.plecis@elvesys.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>École Normale Supérieure-PSL Research University, 24 rue Lhomond, 75005, Paris, France. yong.chen@ens.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Lab Chip</MedlineTA><NlmUniqueID>101128948</NlmUniqueID><ISSNLinking>1473-0189</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>3</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1039/c5lc01459j</ArticleId><ArticleId IdType="pubmed">26952334</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/EpistemeV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000002 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000002 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Ticri/CIDE
|area= EpistemeV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:26952334
|texte= Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:26952334" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a EpistemeV1
| This area was generated with Dilib version V0.6.31. |  |