p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA
Identifieur interne : 000030 ( Pmc/Curation ); précédent : 000029; suivant : 000031p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA
Auteurs : Silvia Sabattini [Italie] ; Federica Savini [Italie] ; Laura Gallina [Italie] ; Alessandra Scagliarini [Italie] ; Patrizia Bassi [Italie] ; Giuliano Bettini [Italie]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
While papillomavirus (PVs) are an established cause of human cancer, few reports have supported a relationship between PV and canine squamous cell carcinomas (SCCs). Human oncogenic PVs lead to an increased expression of the p16 tumor suppressor protein, and the latter can be demonstrated immunohistochemically to support a likely causal relationship between tumor and PV infection. In the present study, archive samples of canine SCC from different anatomical locations were tested by polymerase chain reaction for the presence of PV DNA and by p16 immunohistochemistry. The aims were to investigate the relationship between p16 expression and presence of PV DNA, in order to assess the utility of p16 overexpression as a biomarker of PV infection in canine SCC. A total of 52 SCCs were included. Nine cases (17.3%) showed moderate p16 immunoreactivity, with no association with tumor degree of differentiation, histotype or mitotic activity. The canPVf/FAP64 primers amplified
Url:
DOI: 10.1371/journal.pone.0159687
PubMed: 27441555
PubMed Central: 4956233
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000030
Links to Exploration step
PMC:4956233Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA</title><author><name sortKey="Sabattini, Silvia" sort="Sabattini, Silvia" uniqKey="Sabattini S" first="Silvia" last="Sabattini">Silvia Sabattini</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Savini, Federica" sort="Savini, Federica" uniqKey="Savini F" first="Federica" last="Savini">Federica Savini</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Gallina, Laura" sort="Gallina, Laura" uniqKey="Gallina L" first="Laura" last="Gallina">Laura Gallina</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Scagliarini, Alessandra" sort="Scagliarini, Alessandra" uniqKey="Scagliarini A" first="Alessandra" last="Scagliarini">Alessandra Scagliarini</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Bassi, Patrizia" sort="Bassi, Patrizia" uniqKey="Bassi P" first="Patrizia" last="Bassi">Patrizia Bassi</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Bettini, Giuliano" sort="Bettini, Giuliano" uniqKey="Bettini G" first="Giuliano" last="Bettini">Giuliano Bettini</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO)</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27441555</idno><idno type="pmc">4956233</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956233</idno><idno type="RBID">PMC:4956233</idno><idno type="doi">10.1371/journal.pone.0159687</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000030</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000030</idno><idno type="wicri:Area/Pmc/Curation">000030</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000030</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA</title><author><name sortKey="Sabattini, Silvia" sort="Sabattini, Silvia" uniqKey="Sabattini S" first="Silvia" last="Sabattini">Silvia Sabattini</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Savini, Federica" sort="Savini, Federica" uniqKey="Savini F" first="Federica" last="Savini">Federica Savini</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Gallina, Laura" sort="Gallina, Laura" uniqKey="Gallina L" first="Laura" last="Gallina">Laura Gallina</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Scagliarini, Alessandra" sort="Scagliarini, Alessandra" uniqKey="Scagliarini A" first="Alessandra" last="Scagliarini">Alessandra Scagliarini</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Bassi, Patrizia" sort="Bassi, Patrizia" uniqKey="Bassi P" first="Patrizia" last="Bassi">Patrizia Bassi</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO)</wicri:regionArea></affiliation></author><author><name sortKey="Bettini, Giuliano" sort="Bettini, Giuliano" uniqKey="Bettini G" first="Giuliano" last="Bettini">Giuliano Bettini</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO), Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO)</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>While papillomavirus (PVs) are an established cause of human cancer, few reports have supported a relationship between PV and canine squamous cell carcinomas (SCCs). Human oncogenic PVs lead to an increased expression of the p16 tumor suppressor protein, and the latter can be demonstrated immunohistochemically to support a likely causal relationship between tumor and PV infection. In the present study, archive samples of canine SCC from different anatomical locations were tested by polymerase chain reaction for the presence of PV DNA and by p16 immunohistochemistry. The aims were to investigate the relationship between p16 expression and presence of PV DNA, in order to assess the utility of p16 overexpression as a biomarker of PV infection in canine SCC. A total of 52 SCCs were included. Nine cases (17.3%) showed moderate p16 immunoreactivity, with no association with tumor degree of differentiation, histotype or mitotic activity. The canPVf/FAP64 primers amplified <italic>Canis familiaris</italic> PV-1 DNA from 3 out of 52 tumors (5.8%), one cutaneous, one oral and one tonsillar SCC. There was no association between PV presence and p16 immunostaining. These results do not support a significant role of PVs in the development of canine SCCs. Additionally, PV infection was apparently not the cause of the p16 immunostaining observed in a subset of canine SCCs. A better awareness of p16 level of expression and cellular function in canine cancer may help to define its diagnostic and prognostic role.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Webb, Jl" uniqKey="Webb J">JL Webb</name></author><author><name sortKey="Burns, Re" uniqKey="Burns R">RE Burns</name></author><author><name sortKey="Brown, Hm" uniqKey="Brown H">HM Brown</name></author><author><name sortKey="Leroy, Be" uniqKey="Leroy B">BE LeRoy</name></author><author><name sortKey="Kosarek, Ce" uniqKey="Kosarek C">CE Kosarek</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Mas, A" uniqKey="Mas A">A Mas</name></author><author><name sortKey="Blackwood, L" uniqKey="Blackwood L">L Blackwood</name></author><author><name sortKey="Cripps, P" uniqKey="Cripps P">P Cripps</name></author><author><name sortKey="Murphy, S" uniqKey="Murphy S">S Murphy</name></author><author><name sortKey="De Vos, J" uniqKey="De Vos J">J De Vos</name></author><author><name sortKey="Dervisis, N" uniqKey="Dervisis N">N Dervisis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mellett, S" uniqKey="Mellett S">S Mellett</name></author><author><name sortKey="Verganti, S" uniqKey="Verganti S">S Verganti</name></author><author><name sortKey="Murphy, S" uniqKey="Murphy S">S Murphy</name></author><author><name sortKey="Bowlt, K" uniqKey="Bowlt K">K Bowlt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bostock, De" uniqKey="Bostock D">DE Bostock</name></author><author><name sortKey="Curtis, R" uniqKey="Curtis R">R Curtis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaugg, N" uniqKey="Zaugg N">N Zaugg</name></author><author><name sortKey="Nespeca, G" uniqKey="Nespeca G">G Nespeca</name></author><author><name sortKey="Hauser, B" uniqKey="Hauser B">B Hauser</name></author><author><name sortKey="Ackermann, M" uniqKey="Ackermann M">M Ackermann</name></author><author><name sortKey="Favrot, C" uniqKey="Favrot C">C Favrot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munday, Js" uniqKey="Munday J">JS Munday</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nasir, L" uniqKey="Nasir L">L Nasir</name></author><author><name sortKey="Brandt, S" uniqKey="Brandt S">S Brandt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rector, A" uniqKey="Rector A">A Rector</name></author><author><name sortKey="Van Ranst, M" uniqKey="Van Ranst M">M Van Ranst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lange, Ce" uniqKey="Lange C">CE Lange</name></author><author><name sortKey="Favrot, C" uniqKey="Favrot C">C Favrot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gross, Tl" uniqKey="Gross T">TL Gross</name></author><author><name sortKey="Gross, Tl" uniqKey="Gross T">TL Gross</name></author><author><name sortKey="Ihrke, Pj" uniqKey="Ihrke P">PJ Ihrke</name></author><author><name sortKey="Walder, Ej" uniqKey="Walder E">EJ Walder</name></author><author><name sortKey="Affolter, Vk" uniqKey="Affolter V">VK Affolter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wiggans, Kt" uniqKey="Wiggans K">KT Wiggans</name></author><author><name sortKey="Hoover, Ce" uniqKey="Hoover C">CE Hoover</name></author><author><name sortKey="Ehrhart, Ej" uniqKey="Ehrhart E">EJ Ehrhart</name></author><author><name sortKey="Wobeser, Bk" uniqKey="Wobeser B">BK Wobeser</name></author><author><name sortKey="Cohen, Lb" uniqKey="Cohen L">LB Cohen</name></author><author><name sortKey="Gionfriddo, Jr" uniqKey="Gionfriddo J">JR Gionfriddo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munday, Js" uniqKey="Munday J">JS Munday</name></author><author><name sortKey="O Onnor, Ki" uniqKey="O Onnor K">KI O’Connor</name></author><author><name sortKey="Smits, B" uniqKey="Smits B">B Smits</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luff, Ja" uniqKey="Luff J">JA Luff</name></author><author><name sortKey="Affolter, Vk" uniqKey="Affolter V">VK Affolter</name></author><author><name sortKey="Yeargan, B" uniqKey="Yeargan B">B Yeargan</name></author><author><name sortKey="Moore, Pf" uniqKey="Moore P">PF Moore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luff, J" uniqKey="Luff J">J Luff</name></author><author><name sortKey="Mader, M" uniqKey="Mader M">M Mader</name></author><author><name sortKey="Britton, M" uniqKey="Britton M">M Britton</name></author><author><name sortKey="Fass, J" uniqKey="Fass J">J Fass</name></author><author><name sortKey="Rowland, P" uniqKey="Rowland P">P Rowland</name></author><author><name sortKey="Orr, C" uniqKey="Orr C">C Orr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bregman, Cl" uniqKey="Bregman C">CL Bregman</name></author><author><name sortKey="Hirth, Rs" uniqKey="Hirth R">RS Hirth</name></author><author><name sortKey="Sundberg, Jp" uniqKey="Sundberg J">JP Sundberg</name></author><author><name sortKey="Christensen, Ef" uniqKey="Christensen E">EF Christensen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Watrach, Am" uniqKey="Watrach A">AM Watrach</name></author><author><name sortKey="Small, E" uniqKey="Small E">E Small</name></author><author><name sortKey="Case, Mt" uniqKey="Case M">MT Case</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goldschmidt, Mh" uniqKey="Goldschmidt M">MH Goldschmidt</name></author><author><name sortKey="Kennedy, Js" uniqKey="Kennedy J">JS Kennedy</name></author><author><name sortKey="Kennedy, Dr" uniqKey="Kennedy D">DR Kennedy</name></author><author><name sortKey="Yuan, H" uniqKey="Yuan H">H Yuan</name></author><author><name sortKey="Holt, De" uniqKey="Holt D">DE Holt</name></author><author><name sortKey="Casal, Ml" uniqKey="Casal M">ML Casal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luff, J" uniqKey="Luff J">J Luff</name></author><author><name sortKey="Rowland, P" uniqKey="Rowland P">P Rowland</name></author><author><name sortKey="Mader, M" uniqKey="Mader M">M Mader</name></author><author><name sortKey="Orr, C" uniqKey="Orr C">C Orr</name></author><author><name sortKey="Yuan, H" uniqKey="Yuan H">H Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Teifke, Jp" uniqKey="Teifke J">JP Teifke</name></author><author><name sortKey="Lohr, Cv" uniqKey="Lohr C">CV Löhr</name></author><author><name sortKey="Shirasawa, H" uniqKey="Shirasawa H">H Shirasawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munday, Js" uniqKey="Munday J">JS Munday</name></author><author><name sortKey="Tucker, Rs" uniqKey="Tucker R">RS Tucker</name></author><author><name sortKey="Kiupel, M" uniqKey="Kiupel M">M Kiupel</name></author><author><name sortKey="Harvey, Cj" uniqKey="Harvey C">CJ Harvey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ralli, M" uniqKey="Ralli M">M Ralli</name></author><author><name sortKey="Singh, S" uniqKey="Singh S">S Singh</name></author><author><name sortKey="Yadav, Sp" uniqKey="Yadav S">SP Yadav</name></author><author><name sortKey="Sharma, N" uniqKey="Sharma N">N Sharma</name></author><author><name sortKey="Verma, R" uniqKey="Verma R">R Verma</name></author><author><name sortKey="Sen, R" uniqKey="Sen R">R Sen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meuten, Dj" uniqKey="Meuten D">DJ Meuten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mehanna, H" uniqKey="Mehanna H">H Mehanna</name></author><author><name sortKey="Franklin, N" uniqKey="Franklin N">N Franklin</name></author><author><name sortKey="Compton, N" uniqKey="Compton N">N Compton</name></author><author><name sortKey="Robinson, M" uniqKey="Robinson M">M Robinson</name></author><author><name sortKey="Powell, N" uniqKey="Powell N">N Powell</name></author><author><name sortKey="Biswas Baldwin, N" uniqKey="Biswas Baldwin N">N Biswas-Baldwin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nemec, A" uniqKey="Nemec A">A Nemec</name></author><author><name sortKey="Murphy, B" uniqKey="Murphy B">B Murphy</name></author><author><name sortKey="Kass, Ph" uniqKey="Kass P">PH Kass</name></author><author><name sortKey="Verstraete, Fj" uniqKey="Verstraete F">FJ Verstraete</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munday, Js" uniqKey="Munday J">JS Munday</name></author><author><name sortKey="French, A" uniqKey="French A">A French</name></author><author><name sortKey="Harvey, Cj" uniqKey="Harvey C">CJ Harvey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Forslund, O" uniqKey="Forslund O">O Forslund</name></author><author><name sortKey="Antonsson, A" uniqKey="Antonsson A">A Antonsson</name></author><author><name sortKey="Nordin, P" uniqKey="Nordin P">P Nordin</name></author><author><name sortKey="Stenquist, B" uniqKey="Stenquist B">B Stenquist</name></author><author><name sortKey="Hansson, Bg" uniqKey="Hansson B">BG Hansson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lange, Ce" uniqKey="Lange C">CE Lange</name></author><author><name sortKey="Zollinger, S" uniqKey="Zollinger S">S Zollinger</name></author><author><name sortKey="Tobler, K" uniqKey="Tobler K">K Tobler</name></author><author><name sortKey="Ackermann, M" uniqKey="Ackermann M">M Ackermann</name></author><author><name sortKey="Favrot, C" uniqKey="Favrot C">C Favrot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peyton, Cl" uniqKey="Peyton C">CL Peyton</name></author><author><name sortKey="Gravitt, Pe" uniqKey="Gravitt P">PE Gravitt</name></author><author><name sortKey="Hunt, Wc" uniqKey="Hunt W">WC Hunt</name></author><author><name sortKey="Hundley, Rs" uniqKey="Hundley R">RS Hundley</name></author><author><name sortKey="Zhao, M" uniqKey="Zhao M">M Zhao</name></author><author><name sortKey="Apple, Rj" uniqKey="Apple R">RJ Apple</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waropastrakul, S" uniqKey="Waropastrakul S">S Waropastrakul</name></author><author><name sortKey="Munday, Js" uniqKey="Munday J">JS Munday</name></author><author><name sortKey="French, Af" uniqKey="French A">AF French</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munday, Js" uniqKey="Munday J">JS Munday</name></author><author><name sortKey="Waropastrakul, S" uniqKey="Waropastrakul S">S Waropastrakul</name></author><author><name sortKey="Gibson, I" uniqKey="Gibson I">I Gibson</name></author><author><name sortKey="French, Af" uniqKey="French A">AF French</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nemec, A" uniqKey="Nemec A">A Nemec</name></author><author><name sortKey="Murphy, Bg" uniqKey="Murphy B">BG Murphy</name></author><author><name sortKey="Jordan, Rc" uniqKey="Jordan R">RC Jordan</name></author><author><name sortKey="Kass, Ph" uniqKey="Kass P">PH Kass</name></author><author><name sortKey="Verstraete, Fj" uniqKey="Verstraete F">FJ Verstraete</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munday, Js" uniqKey="Munday J">JS Munday</name></author><author><name sortKey="Dunowska, M" uniqKey="Dunowska M">M Dunowska</name></author><author><name sortKey="Laurie, Re" uniqKey="Laurie R">RE Laurie</name></author><author><name sortKey="Hills, S" uniqKey="Hills S">S Hills</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lange, Ce" uniqKey="Lange C">CE Lange</name></author><author><name sortKey="Ackermann, M" uniqKey="Ackermann M">M Ackermann</name></author><author><name sortKey="Favrot, C" uniqKey="Favrot C">C Favrot</name></author><author><name sortKey="Tobler, K" uniqKey="Tobler K">K Tobler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lange, Ce" uniqKey="Lange C">CE Lange</name></author><author><name sortKey="Tobler, K" uniqKey="Tobler K">K Tobler</name></author><author><name sortKey="Schraner, Em" uniqKey="Schraner E">EM Schraner</name></author><author><name sortKey="Vetsch, E" uniqKey="Vetsch E">E Vetsch</name></author><author><name sortKey="Fischer, Nm" uniqKey="Fischer N">NM Fischer</name></author><author><name sortKey="Ackermann, M" uniqKey="Ackermann M">M Ackermann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Doorslaer, K" uniqKey="Van Doorslaer K">K Van Doorslaer</name></author><author><name sortKey="Tan, Q" uniqKey="Tan Q">Q Tan</name></author><author><name sortKey="Xirasagar, S" uniqKey="Xirasagar S">S Xirasagar</name></author><author><name sortKey="Bandaru, S" uniqKey="Bandaru S">S Bandaru</name></author><author><name sortKey="Gopalan, V" uniqKey="Gopalan V">V Gopalan</name></author><author><name sortKey="Mohamoud, Y" uniqKey="Mohamoud Y">Y Mohamoud</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Antonsson, A" uniqKey="Antonsson A">A Antonsson</name></author><author><name sortKey="Hansson, Bg" uniqKey="Hansson B">BG Hansson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Manos, Mm" uniqKey="Manos M">MM Manos</name></author><author><name sortKey="Ting, Y" uniqKey="Ting Y">Y Ting</name></author><author><name sortKey="Wright, Dk" uniqKey="Wright D">DK Wright</name></author><author><name sortKey="Lewis, Aj" uniqKey="Lewis A">AJ Lewis</name></author><author><name sortKey="Broker, Tr" uniqKey="Broker T">TR Broker</name></author><author><name sortKey="Wolinsky, Sm" uniqKey="Wolinsky S">SM Wolinsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munday, Js" uniqKey="Munday J">JS Munday</name></author><author><name sortKey="Peters Kennedy, J" uniqKey="Peters Kennedy J">J Peters-Kennedy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ogawa, T" uniqKey="Ogawa T">T Ogawa</name></author><author><name sortKey="Tomita, Y" uniqKey="Tomita Y">Y Tomita</name></author><author><name sortKey="Okada, M" uniqKey="Okada M">M Okada</name></author><author><name sortKey="Shinozaki, K" uniqKey="Shinozaki K">K Shinozaki</name></author><author><name sortKey="Kubonoya, H" uniqKey="Kubonoya H">H Kubonoya</name></author><author><name sortKey="Kaiho, I" uniqKey="Kaiho I">I Kaiho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Little, Se" uniqKey="Little S">SE Little</name></author><author><name sortKey="Vuononvirta, R" uniqKey="Vuononvirta R">R Vuononvirta</name></author><author><name sortKey="Reis Filho, Js" uniqKey="Reis Filho J">JS Reis-Filho</name></author><author><name sortKey="Natrajan, R" uniqKey="Natrajan R">R Natrajan</name></author><author><name sortKey="Iravani, M" uniqKey="Iravani M">M Iravani</name></author><author><name sortKey="Fenwick, K" uniqKey="Fenwick K">K Fenwick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boscolo Rizzo, P" uniqKey="Boscolo Rizzo P">P Boscolo-Rizzo</name></author><author><name sortKey="Pawlita, M" uniqKey="Pawlita M">M Pawlita</name></author><author><name sortKey="Holzinger, D" uniqKey="Holzinger D">D Holzinger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Porcellato, I" uniqKey="Porcellato I">I Porcellato</name></author><author><name sortKey="Brachelente, C" uniqKey="Brachelente C">C Brachelente</name></author><author><name sortKey="Guelfi, G" uniqKey="Guelfi G">G Guelfi</name></author><author><name sortKey="Reginato, A" uniqKey="Reginato A">A Reginato</name></author><author><name sortKey="Sforna, M" uniqKey="Sforna M">M Sforna</name></author><author><name sortKey="Bongiovanni, L" uniqKey="Bongiovanni L">L Bongiovanni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Longworth, Ms" uniqKey="Longworth M">MS Longworth</name></author><author><name sortKey="Laimins, La" uniqKey="Laimins L">LA Laimins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Firzlaff, Jm" uniqKey="Firzlaff J">JM Firzlaff</name></author><author><name sortKey="Kiviat, Nb" uniqKey="Kiviat N">NB Kiviat</name></author><author><name sortKey="Beckmann, Am" uniqKey="Beckmann A">AM Beckmann</name></author><author><name sortKey="Jenison, Sa" uniqKey="Jenison S">SA Jenison</name></author><author><name sortKey="Galloway, Da" uniqKey="Galloway D">DA Galloway</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doorbar, J" uniqKey="Doorbar J">J Doorbar</name></author><author><name sortKey="Quint, W" uniqKey="Quint W">W Quint</name></author><author><name sortKey="Banks, L" uniqKey="Banks L">L Banks</name></author><author><name sortKey="Bravo, Ig" uniqKey="Bravo I">IG Bravo</name></author><author><name sortKey="Stoler, M" uniqKey="Stoler M">M Stoler</name></author><author><name sortKey="Broker, Tr" uniqKey="Broker T">TR Broker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smeets, Sj" uniqKey="Smeets S">SJ Smeets</name></author><author><name sortKey="Hesselink, At" uniqKey="Hesselink A">AT Hesselink</name></author><author><name sortKey="Speel, Ej" uniqKey="Speel E">EJ Speel</name></author><author><name sortKey="Haesevoets, A" uniqKey="Haesevoets A">A Haesevoets</name></author><author><name sortKey="Snijders, Pj" uniqKey="Snijders P">PJ Snijders</name></author><author><name sortKey="Pawlita, M" uniqKey="Pawlita M">M Pawlita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gillison, Ml" uniqKey="Gillison M">ML Gillison</name></author><author><name sortKey="Koch, Wm" uniqKey="Koch W">WM Koch</name></author><author><name sortKey="Capone, Rb" uniqKey="Capone R">RB Capone</name></author><author><name sortKey="Spafford, M" uniqKey="Spafford M">M Spafford</name></author><author><name sortKey="Westra, Wh" uniqKey="Westra W">WH Westra</name></author><author><name sortKey="Wu, L" uniqKey="Wu L">L Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Milde Langosch, K" uniqKey="Milde Langosch K">K Milde-Langosch</name></author><author><name sortKey="Bamberger, Am" uniqKey="Bamberger A">AM Bamberger</name></author><author><name sortKey="Rieck, G" uniqKey="Rieck G">G Rieck</name></author><author><name sortKey="Kelp, B" uniqKey="Kelp B">B Kelp</name></author><author><name sortKey="Loning, T" uniqKey="Loning T">T Loning</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arifin, Mt" uniqKey="Arifin M">MT Arifin</name></author><author><name sortKey="Hama, S" uniqKey="Hama S">S Hama</name></author><author><name sortKey="Kajiwara, Y" uniqKey="Kajiwara Y">Y Kajiwara</name></author><author><name sortKey="Sugiyama, K" uniqKey="Sugiyama K">K Sugiyama</name></author><author><name sortKey="Saito, T" uniqKey="Saito T">T Saito</name></author><author><name sortKey="Matsuura, S" uniqKey="Matsuura S">S Matsuura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lam, Ak" uniqKey="Lam A">AK Lam</name></author><author><name sortKey="Ong, K" uniqKey="Ong K">K Ong</name></author><author><name sortKey="Giv, Mj" uniqKey="Giv M">MJ Giv</name></author><author><name sortKey="Ho, Yh" uniqKey="Ho Y">YH Ho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Romagosa, C" uniqKey="Romagosa C">C Romagosa</name></author><author><name sortKey="Simonetti, S" uniqKey="Simonetti S">S Simonetti</name></author><author><name sortKey="L Pez Vicente, L" uniqKey="L Pez Vicente L">L López-Vicente</name></author><author><name sortKey="Mazo, A" uniqKey="Mazo A">A Mazo</name></author><author><name sortKey="Lleonart, Me" uniqKey="Lleonart M">ME Lleonart</name></author><author><name sortKey="Castellvi, J" uniqKey="Castellvi J">J Castellvi</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27441555</article-id><article-id pub-id-type="pmc">4956233</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0159687</article-id><article-id pub-id-type="publisher-id">PONE-D-16-10480</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Oncology</subject><subj-group><subject>Cancers and Neoplasms</subject><subj-group><subject>Carcinomas</subject><subj-group><subject>Squamous Cell Carcinomas</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Animals</subject><subj-group><subject>Vertebrates</subject><subj-group><subject>Amniotes</subject><subj-group><subject>Mammals</subject><subj-group><subject>Dogs</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Specimen Preparation and Treatment</subject><subj-group><subject>Staining</subject><subj-group><subject>Immunostaining</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Molecular Biology</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Artificial Gene Amplification and Extension</subject><subj-group><subject>Polymerase Chain Reaction</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Artificial Gene Amplification and Extension</subject><subj-group><subject>Polymerase Chain Reaction</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Histology</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Histology</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Histochemistry and Cytochemistry Techniques</subject><subj-group><subject>Immunohistochemistry Techniques</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Immunologic Techniques</subject><subj-group><subject>Immunohistochemistry Techniques</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Genetics</subject><subj-group><subject>DNA</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Nucleic Acids</subject><subj-group><subject>DNA</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Genetics</subject><subj-group><subject>Gene Amplification</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA</article-title><alt-title alt-title-type="running-head">The Role of Papillomavirus in Canine Squamous Cell Carcinoma</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Sabattini</surname><given-names>Silvia</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Savini</surname><given-names>Federica</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gallina</surname><given-names>Laura</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Scagliarini</surname><given-names>Alessandra</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bassi</surname><given-names>Patrizia</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0003-1857-7329</contrib-id><name><surname>Bettini</surname><given-names>Giuliano</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Pathology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia (BO), Italy</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Virology Division, Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>McLaughlin-Drubin</surname><given-names>Margaret</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Brigham and Women's Hospital/Harvard Medical School, UNITED STATES</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: SS GB. Performed the experiments: SS FS LG AS PB. Analyzed the data: SS. Contributed reagents/materials/analysis tools: AS. Wrote the paper: SS LG GB.</p></fn><corresp id="cor001">* E-mail: <email>giuliano.bettini@unibo.it</email></corresp></author-notes><pub-date pub-type="epub"><day>21</day><month>7</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>11</volume><issue>7</issue><elocation-id>e0159687</elocation-id><history><date date-type="received"><day>12</day><month>3</month><year>2016</year></date><date date-type="accepted"><day>5</day><month>7</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 Sabattini et al</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Sabattini et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="pone.0159687.pdf"></self-uri><abstract><p>While papillomavirus (PVs) are an established cause of human cancer, few reports have supported a relationship between PV and canine squamous cell carcinomas (SCCs). Human oncogenic PVs lead to an increased expression of the p16 tumor suppressor protein, and the latter can be demonstrated immunohistochemically to support a likely causal relationship between tumor and PV infection. In the present study, archive samples of canine SCC from different anatomical locations were tested by polymerase chain reaction for the presence of PV DNA and by p16 immunohistochemistry. The aims were to investigate the relationship between p16 expression and presence of PV DNA, in order to assess the utility of p16 overexpression as a biomarker of PV infection in canine SCC. A total of 52 SCCs were included. Nine cases (17.3%) showed moderate p16 immunoreactivity, with no association with tumor degree of differentiation, histotype or mitotic activity. The canPVf/FAP64 primers amplified <italic>Canis familiaris</italic> PV-1 DNA from 3 out of 52 tumors (5.8%), one cutaneous, one oral and one tonsillar SCC. There was no association between PV presence and p16 immunostaining. These results do not support a significant role of PVs in the development of canine SCCs. Additionally, PV infection was apparently not the cause of the p16 immunostaining observed in a subset of canine SCCs. A better awareness of p16 level of expression and cellular function in canine cancer may help to define its diagnostic and prognostic role.</p></abstract><funding-group><award-group id="award001"><funding-source><institution>FARB—Finanziamenti di Ateneo alla ricerca di base, University of Bologna</institution></funding-source><principal-award-recipient><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0003-1857-7329</contrib-id><name><surname>Bettini</surname><given-names>Giuliano</given-names></name></principal-award-recipient></award-group><funding-statement>This work was supported by grants from the University of Bologna (FARB—Finanziamenti di Ateneo alla ricerca di base). The funding source had no involvement in the study design or in the writing of the report.</funding-statement></funding-group><counts><fig-count count="2"></fig-count><table-count count="0"></table-count><page-count count="11"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information files.</p></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/EpistemeV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000030 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000030 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Ticri/CIDE
|area= EpistemeV1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:4956233
|texte= p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:27441555" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a EpistemeV1
| This area was generated with Dilib version V0.6.31. |  |