The Subcellular Localisation of the Human Papillomavirus (HPV) 16 E7 Protein in Cervical Cancer Cells and Its Perturbation by RNA Aptamers
Identifieur interne : 000020 ( Pmc/Checkpoint ); précédent : 000019; suivant : 000021The Subcellular Localisation of the Human Papillomavirus (HPV) 16 E7 Protein in Cervical Cancer Cells and Its Perturbation by RNA Aptamers
Auteurs : Özlem Cesur ; Clare Nicol ; Helen Groves ; Jamel Mankouri ; George Eric Blair ; Nicola J. StonehouseSource :
- Viruses [ 1999-4915 ] ; 2015.
Abstract
Human papillomavirus (HPV) is the most common viral infection of the reproductive tract, affecting both men and women. High-risk oncogenic types are responsible for almost 90% of anogenital and oropharyngeal cancers including cervical cancer. Some of the HPV “early” genes, particularly E6 and E7, are known to act as oncogenes that promote tumour growth and malignant transformation. Most notably, HPV-16 E7 interacts with the tumour suppressor protein pRb, promoting its degradation, leading to cell cycle dysregulation in infected cells. We have previously shown that an RNA aptamer (termed A2) selectively binds to HPV16 E7 and is able to induce apoptosis in HPV16-transformed cervical carcinoma cell lines (SiHa) through reduction of E7 levels. In this study, we investigated the effects of the A2 aptamer on E7 localisation in order to define its effects on E7 activity. We demonstrate for the first time that E7 localised to the plasma membrane. In addition, we show that A2 enhanced E7 localisation in the ER and that the A2-mediated reduction of E7 was not associated with proteasomal degradation. These data suggest that A2 perturbs normal E7 trafficking through promoting E7 ER retention.
Url:
DOI: 10.3390/v7072780
PubMed: 26131956
PubMed Central: 4517109
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Subcellular Localisation of the Human Papillomavirus (HPV) 16 E7 Protein in Cervical Cancer Cells and Its Perturbation by RNA Aptamers</title><author><name sortKey="Cesur, Ozlem" sort="Cesur, Ozlem" uniqKey="Cesur O" first="Özlem" last="Cesur">Özlem Cesur</name></author><author><name sortKey="Nicol, Clare" sort="Nicol, Clare" uniqKey="Nicol C" first="Clare" last="Nicol">Clare Nicol</name></author><author><name sortKey="Groves, Helen" sort="Groves, Helen" uniqKey="Groves H" first="Helen" last="Groves">Helen Groves</name></author><author><name sortKey="Mankouri, Jamel" sort="Mankouri, Jamel" uniqKey="Mankouri J" first="Jamel" last="Mankouri">Jamel Mankouri</name></author><author><name sortKey="Blair, George Eric" sort="Blair, George Eric" uniqKey="Blair G" first="George Eric" last="Blair">George Eric Blair</name></author><author><name sortKey="Stonehouse, Nicola J" sort="Stonehouse, Nicola J" uniqKey="Stonehouse N" first="Nicola J." last="Stonehouse">Nicola J. Stonehouse</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26131956</idno><idno type="pmc">4517109</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517109</idno><idno type="RBID">PMC:4517109</idno><idno type="doi">10.3390/v7072780</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000013</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000013</idno><idno type="wicri:Area/Pmc/Curation">000013</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000013</idno><idno type="wicri:Area/Pmc/Checkpoint">000020</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000020</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Subcellular Localisation of the Human Papillomavirus (HPV) 16 E7 Protein in Cervical Cancer Cells and Its Perturbation by RNA Aptamers</title><author><name sortKey="Cesur, Ozlem" sort="Cesur, Ozlem" uniqKey="Cesur O" first="Özlem" last="Cesur">Özlem Cesur</name></author><author><name sortKey="Nicol, Clare" sort="Nicol, Clare" uniqKey="Nicol C" first="Clare" last="Nicol">Clare Nicol</name></author><author><name sortKey="Groves, Helen" sort="Groves, Helen" uniqKey="Groves H" first="Helen" last="Groves">Helen Groves</name></author><author><name sortKey="Mankouri, Jamel" sort="Mankouri, Jamel" uniqKey="Mankouri J" first="Jamel" last="Mankouri">Jamel Mankouri</name></author><author><name sortKey="Blair, George Eric" sort="Blair, George Eric" uniqKey="Blair G" first="George Eric" last="Blair">George Eric Blair</name></author><author><name sortKey="Stonehouse, Nicola J" sort="Stonehouse, Nicola J" uniqKey="Stonehouse N" first="Nicola J." last="Stonehouse">Nicola J. Stonehouse</name></author></analytic><series><title level="j">Viruses</title><idno type="eISSN">1999-4915</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Human papillomavirus (HPV) is the most common viral infection of the reproductive tract, affecting both men and women. High-risk oncogenic types are responsible for almost 90% of anogenital and oropharyngeal cancers including cervical cancer. Some of the HPV “early” genes, particularly E6 and E7, are known to act as oncogenes that promote tumour growth and malignant transformation. Most notably, HPV-16 E7 interacts with the tumour suppressor protein pRb, promoting its degradation, leading to cell cycle dysregulation in infected cells. We have previously shown that an RNA aptamer (termed A2) selectively binds to HPV16 E7 and is able to induce apoptosis in HPV16-transformed cervical carcinoma cell lines (SiHa) through reduction of E7 levels. In this study, we investigated the effects of the A2 aptamer on E7 localisation in order to define its effects on E7 activity. We demonstrate for the first time that E7 localised to the plasma membrane. In addition, we show that A2 enhanced E7 localisation in the ER and that the A2-mediated reduction of E7 was not associated with proteasomal degradation. These data suggest that A2 perturbs normal E7 trafficking through promoting E7 ER retention.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Bernard, H U" uniqKey="Bernard H">H.U. Bernard</name></author><author><name sortKey="Burk, R D" uniqKey="Burk R">R.D. Burk</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Van Doorslaer, K" uniqKey="Van Doorslaer K">K. van Doorslaer</name></author><author><name sortKey="Zur Hausen, H" uniqKey="Zur Hausen H">H. zur Hausen</name></author><author><name sortKey="De Villiers, E M" uniqKey="De Villiers E">E.M. de Villiers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zur Hausen, H" uniqKey="Zur Hausen H">H. Zur Hausen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Naucler, P" uniqKey="Naucler P">P. Naucler</name></author><author><name sortKey="Mabota Da Costa, F" uniqKey="Mabota Da Costa F">F. Mabota da Costa</name></author><author><name sortKey="Da Costa, J L" uniqKey="Da Costa J">J.L. da Costa</name></author><author><name sortKey="Ljungberg, O" uniqKey="Ljungberg O">O. Ljungberg</name></author><author><name sortKey="Bugalho, A" uniqKey="Bugalho A">A. Bugalho</name></author><author><name sortKey="Dillner, J" uniqKey="Dillner J">J. Dillner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosch, F X" uniqKey="Bosch F">F.X. Bosch</name></author><author><name sortKey="Lorincz, A" uniqKey="Lorincz A">A. Lorincz</name></author><author><name sortKey="Mu Oz, N" uniqKey="Mu Oz N">N. Muñoz</name></author><author><name sortKey="Meijer, C J" uniqKey="Meijer C">C.J. Meijer</name></author><author><name sortKey="Shah, K V" uniqKey="Shah K">K.V. Shah</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Mclaughlin Drubin, M E" uniqKey="Mclaughlin Drubin M">M.E. McLaughlin-Drubin</name></author><author><name sortKey="Munger, K" uniqKey="Munger K">K. Munger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, X" uniqKey="Liu X">X. Liu</name></author><author><name sortKey="Clements, A" uniqKey="Clements A">A. Clements</name></author><author><name sortKey="Zhao, K" uniqKey="Zhao K">K. Zhao</name></author><author><name sortKey="Marmorstein, R" uniqKey="Marmorstein R">R. Marmorstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ohlenschlager, O" uniqKey="Ohlenschlager O">O. Ohlenschlager</name></author><author><name sortKey="Seiboth, T" uniqKey="Seiboth T">T. Seiboth</name></author><author><name sortKey="Zengerling, H" uniqKey="Zengerling H">H. Zengerling</name></author><author><name sortKey="Briese, L" uniqKey="Briese L">L. Briese</name></author><author><name sortKey="Marchanka, A" uniqKey="Marchanka A">A. Marchanka</name></author><author><name sortKey="Ramachandran, R" uniqKey="Ramachandran R">R. Ramachandran</name></author><author><name sortKey="Baum, M" uniqKey="Baum M">M. Baum</name></author><author><name sortKey="Korbas, M" uniqKey="Korbas M">M. Korbas</name></author><author><name sortKey="Meyer Klaucke, W" uniqKey="Meyer Klaucke W">W. Meyer-Klaucke</name></author><author><name sortKey="Durst, M" uniqKey="Durst M">M. Dürst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mcintyre, M C" uniqKey="Mcintyre M">M.C. McIntyre</name></author><author><name sortKey="Frattini, M G" uniqKey="Frattini M">M.G. Frattini</name></author><author><name sortKey="Grossman, S R" uniqKey="Grossman S">S.R. Grossman</name></author><author><name sortKey="Laimins, L A" uniqKey="Laimins L">L.A. Laimins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Clements, A" uniqKey="Clements A">A. Clements</name></author><author><name sortKey="Johnston, K" uniqKey="Johnston K">K. Johnston</name></author><author><name sortKey="Mazzarelli, J M" uniqKey="Mazzarelli J">J.M. Mazzarelli</name></author><author><name sortKey="Ricciardi, R P" uniqKey="Ricciardi R">R.P. Ricciardi</name></author><author><name sortKey="Marmorstein, R" uniqKey="Marmorstein R">R. Marmorstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alonso, L G" uniqKey="Alonso L">L.G. Alonso</name></author><author><name sortKey="Garcia Alai, M M" uniqKey="Garcia Alai M">M.M. García-Alai</name></author><author><name sortKey="Smal, C" uniqKey="Smal C">C. Smal</name></author><author><name sortKey="Centeno, J M" uniqKey="Centeno J">J.M. Centeno</name></author><author><name sortKey="Iacono, R" uniqKey="Iacono R">R. Iacono</name></author><author><name sortKey="Casta O, E" uniqKey="Casta O E">E. Castaño</name></author><author><name sortKey="Gualfetti, P" uniqKey="Gualfetti P">P. Gualfetti</name></author><author><name sortKey="De Prat Gay, G" uniqKey="De Prat Gay G">G. de Prat-Gay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smotkin, D" uniqKey="Smotkin D">D. Smotkin</name></author><author><name sortKey="Wettstein, F O" uniqKey="Wettstein F">F.O. Wettstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith Mccune, K" uniqKey="Smith Mccune K">K. Smith-McCune</name></author><author><name sortKey="Kalman, D" uniqKey="Kalman D">D. Kalman</name></author><author><name sortKey="Robbins, C" uniqKey="Robbins C">C. Robbins</name></author><author><name sortKey="Shivakumar, S" uniqKey="Shivakumar S">S. Shivakumar</name></author><author><name sortKey="Yuschenkoff, L" uniqKey="Yuschenkoff L">L. Yuschenkoff</name></author><author><name sortKey="Bishop, J M" uniqKey="Bishop J">J.M. Bishop</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zatsepina, O" uniqKey="Zatsepina O">O. Zatsepina</name></author><author><name sortKey="Braspenning, J" uniqKey="Braspenning J">J. Braspenning</name></author><author><name sortKey="Robberson, D" uniqKey="Robberson D">D. Robberson</name></author><author><name sortKey="Hajibagheri, M A" uniqKey="Hajibagheri M">M.A. Hajibagheri</name></author><author><name sortKey="Blight, K J" uniqKey="Blight K">K.J. Blight</name></author><author><name sortKey="Ely, S" uniqKey="Ely S">S. Ely</name></author><author><name sortKey="Hibma, M" uniqKey="Hibma M">M. Hibma</name></author><author><name sortKey="Spitkovsky, D" uniqKey="Spitkovsky D">D. Spitkovsky</name></author><author><name sortKey="Trendelenburg, M" uniqKey="Trendelenburg M">M. Trendelenburg</name></author><author><name sortKey="Crawford, L" uniqKey="Crawford L">L. Crawford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dantur, K" uniqKey="Dantur K">K. Dantur</name></author><author><name sortKey="Alonso, L" uniqKey="Alonso L">L. Alonso</name></author><author><name sortKey="Casta O, E" uniqKey="Casta O E">E. Castaño</name></author><author><name sortKey="Morelli, L" uniqKey="Morelli L">L. Morelli</name></author><author><name sortKey="Centeno Crowley, J M" uniqKey="Centeno Crowley J">J.M. Centeno-Crowley</name></author><author><name sortKey="Vighi, S" uniqKey="Vighi S">S. Vighi</name></author><author><name sortKey="De Prat Gay, G" uniqKey="De Prat Gay G">G. de Prat-Gay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Knapp, A A" uniqKey="Knapp A">A.A. Knapp</name></author><author><name sortKey="Mcmanus, P M" uniqKey="Mcmanus P">P.M. McManus</name></author><author><name sortKey="Bockstall, K" uniqKey="Bockstall K">K. Bockstall</name></author><author><name sortKey="Moroianu, J" uniqKey="Moroianu J">J. Moroianu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laurson, J" uniqKey="Laurson J">J. Laurson</name></author><author><name sortKey="Raj, K" uniqKey="Raj K">K. Raj</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tuerk, C" uniqKey="Tuerk C">C. Tuerk</name></author><author><name sortKey="Gold, L" uniqKey="Gold L">L. Gold</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Keefe, A D" uniqKey="Keefe A">A.D. Keefe</name></author><author><name sortKey="Cload, S T" uniqKey="Cload S">S.T. Cload</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blind, M" uniqKey="Blind M">M. Blind</name></author><author><name sortKey="Blank, M" uniqKey="Blank M">M. Blank</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belyaeva, T A" uniqKey="Belyaeva T">T.A. Belyaeva</name></author><author><name sortKey="Nicol, C" uniqKey="Nicol C">C. Nicol</name></author><author><name sortKey="Cesur, O" uniqKey="Cesur O">O. Cesur</name></author><author><name sortKey="Trave, G" uniqKey="Trave G">G. Travé</name></author><author><name sortKey="Blair, G E" uniqKey="Blair G">G.E. Blair</name></author><author><name sortKey="Stonehouse, N J" uniqKey="Stonehouse N">N.J. Stonehouse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nicol, C" uniqKey="Nicol C">C. Nicol</name></author><author><name sortKey="Bunka, D H" uniqKey="Bunka D">D.H. Bunka</name></author><author><name sortKey="Blair, G E" uniqKey="Blair G">G.E. Blair</name></author><author><name sortKey="Stonehouse, N J" uniqKey="Stonehouse N">N.J. Stonehouse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nicol, C" uniqKey="Nicol C">C. Nicol</name></author><author><name sortKey="Cesur, O" uniqKey="Cesur O">Ö. Cesur</name></author><author><name sortKey="Forrest, S" uniqKey="Forrest S">S. Forrest</name></author><author><name sortKey="Belyaeva, T A" uniqKey="Belyaeva T">T.A. Belyaeva</name></author><author><name sortKey="Bunka, D H" uniqKey="Bunka D">D.H. Bunka</name></author><author><name sortKey="Blair, G E" uniqKey="Blair G">G.E. Blair</name></author><author><name sortKey="Stonehouse, N J" uniqKey="Stonehouse N">N.J. Stonehouse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ellingham, M" uniqKey="Ellingham M">M. Ellingham</name></author><author><name sortKey="Bunka, D H" uniqKey="Bunka D">D.H. Bunka</name></author><author><name sortKey="Rowlands, D J" uniqKey="Rowlands D">D.J. Rowlands</name></author><author><name sortKey="Stonehouse, N J" uniqKey="Stonehouse N">N.J. Stonehouse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Forrest, S" uniqKey="Forrest S">S. Forrest</name></author><author><name sortKey="Lear, Z" uniqKey="Lear Z">Z. Lear</name></author><author><name sortKey="Herod, M R" uniqKey="Herod M">M.R. Herod</name></author><author><name sortKey="Ryan, M" uniqKey="Ryan M">M. Ryan</name></author><author><name sortKey="Rowlands, D J" uniqKey="Rowlands D">D.J. Rowlands</name></author><author><name sortKey="Stonehouse, N J" uniqKey="Stonehouse N">N.J. Stonehouse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bellecave, P" uniqKey="Bellecave P">P. Bellecave</name></author><author><name sortKey="Cazenave, C" uniqKey="Cazenave C">C. Cazenave</name></author><author><name sortKey="Rumi, J" uniqKey="Rumi J">J. Rumi</name></author><author><name sortKey="Staedel, C" uniqKey="Staedel C">C. Staedel</name></author><author><name sortKey="Cosnefroy, O" uniqKey="Cosnefroy O">O. Cosnefroy</name></author><author><name sortKey="Andreola, M L" uniqKey="Andreola M">M.L. Andreola</name></author><author><name sortKey="Ventura, M" uniqKey="Ventura M">M. Ventura</name></author><author><name sortKey="Tarrago Litvak, L" uniqKey="Tarrago Litvak L">L. Tarrago-Litvak</name></author><author><name sortKey="Astier Gin, T" uniqKey="Astier Gin T">T. Astier-Gin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Biroccio, A" uniqKey="Biroccio A">A. Biroccio</name></author><author><name sortKey="Hamm, J" uniqKey="Hamm J">J. Hamm</name></author><author><name sortKey="Incitti, I" uniqKey="Incitti I">I. Incitti</name></author><author><name sortKey="De Francesco, R" uniqKey="De Francesco R">R. De Francesco</name></author><author><name sortKey="Tomei, L" uniqKey="Tomei L">L. Tomei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bates, P J" uniqKey="Bates P">P.J. Bates</name></author><author><name sortKey="Kahlon, J B" uniqKey="Kahlon J">J.B. Kahlon</name></author><author><name sortKey="Thomas, S D" uniqKey="Thomas S">S.D. Thomas</name></author><author><name sortKey="Trent, J O" uniqKey="Trent J">J.O. Trent</name></author><author><name sortKey="Miller, D M" uniqKey="Miller D">D.M. Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sayyed, S G" uniqKey="Sayyed S">S.G. Sayyed</name></author><author><name sortKey="H Gele, H" uniqKey="H Gele H">H. Hägele</name></author><author><name sortKey="Kulkarni, O P" uniqKey="Kulkarni O">O.P. Kulkarni</name></author><author><name sortKey="Endlich, K" uniqKey="Endlich K">K. Endlich</name></author><author><name sortKey="Segerer, S" uniqKey="Segerer S">S. Segerer</name></author><author><name sortKey="Eulberg, D" uniqKey="Eulberg D">D. Eulberg</name></author><author><name sortKey="Klussmann, S" uniqKey="Klussmann S">S. Klussmann</name></author><author><name sortKey="Anders, H J" uniqKey="Anders H">H.J. Anders</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doble, R" uniqKey="Doble R">R. Doble</name></author><author><name sortKey="Mcdermott, M F" uniqKey="Mcdermott M">M.F. McDermott</name></author><author><name sortKey="Cesur, O" uniqKey="Cesur O">O. Cesur</name></author><author><name sortKey="Stonehouse, N J" uniqKey="Stonehouse N">N.J. Stonehouse</name></author><author><name sortKey="Wittmann, M" uniqKey="Wittmann M">M. Wittmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nafissi, N" uniqKey="Nafissi N">N. Nafissi</name></author><author><name sortKey="Alqawlaq, S" uniqKey="Alqawlaq S">S. Alqawlaq</name></author><author><name sortKey="Lee, E A" uniqKey="Lee E">E.A. Lee</name></author><author><name sortKey="Foldvari, M" uniqKey="Foldvari M">M. Foldvari</name></author><author><name sortKey="Spagnuolo, P A" uniqKey="Spagnuolo P">P.A. Spagnuolo</name></author><author><name sortKey="Slavcev, R A" uniqKey="Slavcev R">R.A. Slavcev</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rossignol, J F" uniqKey="Rossignol J">J.F. Rossignol</name></author><author><name sortKey="La Frazia, S" uniqKey="La Frazia S">S. La Frazia</name></author><author><name sortKey="Chiappa, L" uniqKey="Chiappa L">L. Chiappa</name></author><author><name sortKey="Ciucci, A" uniqKey="Ciucci A">A. Ciucci</name></author><author><name sortKey="Santoro, M G" uniqKey="Santoro M">M.G. Santoro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mankouri, J" uniqKey="Mankouri J">J. Mankouri</name></author><author><name sortKey="Taneja, T K" uniqKey="Taneja T">T.K. Taneja</name></author><author><name sortKey="Smith, A J" uniqKey="Smith A">A.J. Smith</name></author><author><name sortKey="Ponnambalam, S" uniqKey="Ponnambalam S">S. Ponnambalam</name></author><author><name sortKey="Sivaprasadarao, A" uniqKey="Sivaprasadarao A">A. Sivaprasadarao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grant, B D" uniqKey="Grant B">B.D. Grant</name></author><author><name sortKey="Donaldson, J G" uniqKey="Donaldson J">J.G. Donaldson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Greenfield, I" uniqKey="Greenfield I">I. Greenfield</name></author><author><name sortKey="Nickerson, J" uniqKey="Nickerson J">J. Nickerson</name></author><author><name sortKey="Penman, S" uniqKey="Penman S">S. Penman</name></author><author><name sortKey="Stanley, M" uniqKey="Stanley M">M. Stanley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reinstein, E" uniqKey="Reinstein E">E. Reinstein</name></author><author><name sortKey="Scheffner, M" uniqKey="Scheffner M">M. Scheffner</name></author><author><name sortKey="Oren, M" uniqKey="Oren M">M. Oren</name></author><author><name sortKey="Ciechanover, A" uniqKey="Ciechanover A">A. Ciechanover</name></author><author><name sortKey="Schwartz, A" uniqKey="Schwartz A">A. Schwartz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dominska, M" uniqKey="Dominska M">M. Dominska</name></author><author><name sortKey="Dykxhoorn, D M" uniqKey="Dykxhoorn D">D.M. Dykxhoorn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valdovinos Torres, H" uniqKey="Valdovinos Torres H">H. Valdovinos-Torres</name></author><author><name sortKey="Orozco Morales, M" uniqKey="Orozco Morales M">M. Orozco-Morales</name></author><author><name sortKey="Pedroza Saavedra, A" uniqKey="Pedroza Saavedra A">A. Pedroza-Saavedra</name></author><author><name sortKey="Padilla Noriega, L" uniqKey="Padilla Noriega L">L. Padilla-Noriega</name></author><author><name sortKey="Esquivel Guadarrama, F" uniqKey="Esquivel Guadarrama F">F. Esquivel-Guadarrama</name></author><author><name sortKey="Gutierrez Xicotencatl, L" uniqKey="Gutierrez Xicotencatl L">L. Gutierrez-Xicotencatl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Casagrande, R" uniqKey="Casagrande R">R. Casagrande</name></author><author><name sortKey="Stern, P" uniqKey="Stern P">P. Stern</name></author><author><name sortKey="Diehn, M" uniqKey="Diehn M">M. Diehn</name></author><author><name sortKey="Shamu, C" uniqKey="Shamu C">C. Shamu</name></author><author><name sortKey="Osario, M" uniqKey="Osario M">M. Osario</name></author><author><name sortKey="Zu Iga, M" uniqKey="Zu Iga M">M. Zúñiga</name></author><author><name sortKey="Brown, P O" uniqKey="Brown P">P.O. Brown</name></author><author><name sortKey="Ploegh, H" uniqKey="Ploegh H">H. Ploegh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, C Y" uniqKey="Liu C">C.Y. Liu</name></author><author><name sortKey="Kaufman, R J" uniqKey="Kaufman R">R.J. Kaufman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Travers, K J" uniqKey="Travers K">K.J. Travers</name></author><author><name sortKey="Patil, C K" uniqKey="Patil C">C.K. Patil</name></author><author><name sortKey="Wodicka, L" uniqKey="Wodicka L">L. Wodicka</name></author><author><name sortKey="Lockhart, D J" uniqKey="Lockhart D">D.J. Lockhart</name></author><author><name sortKey="Weissman, J S" uniqKey="Weissman J">J.S. Weissman</name></author><author><name sortKey="Walter, P" uniqKey="Walter P">P. Walter</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Viruses</journal-id><journal-id journal-id-type="iso-abbrev">Viruses</journal-id><journal-id journal-id-type="publisher-id">viruses</journal-id><journal-title-group><journal-title>Viruses</journal-title></journal-title-group><issn pub-type="epub">1999-4915</issn><publisher><publisher-name>MDPI</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26131956</article-id><article-id pub-id-type="pmc">4517109</article-id><article-id pub-id-type="doi">10.3390/v7072780</article-id><article-id pub-id-type="publisher-id">viruses-07-02780</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>The Subcellular Localisation of the Human Papillomavirus (HPV) 16 E7 Protein in Cervical Cancer Cells and Its Perturbation by RNA Aptamers</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Cesur</surname><given-names>Özlem</given-names></name></contrib><contrib contrib-type="author"><name><surname>Nicol</surname><given-names>Clare</given-names></name></contrib><contrib contrib-type="author"><name><surname>Groves</surname><given-names>Helen</given-names></name></contrib><contrib contrib-type="author"><name><surname>Mankouri</surname><given-names>Jamel</given-names></name></contrib><contrib contrib-type="author"><name><surname>Blair</surname><given-names>George Eric</given-names></name></contrib><contrib contrib-type="author"><name><surname>Stonehouse</surname><given-names>Nicola J.</given-names></name><xref rid="c1-viruses-07-02780" ref-type="corresp">*</xref></contrib></contrib-group><contrib-group><contrib contrib-type="editor"><name><surname>Parish</surname><given-names>Joanna</given-names></name><role>Academic Editor</role></contrib></contrib-group><aff id="af1-viruses-07-02780">School of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK; E-Mails:<email>bs09oc@leeds.ac.uk</email> (Ö.C.);<email>c.nicol@leeds.ac.uk</email> (C.N.);<email>h.groves14@imperial.ac.uk</email> (H.G.);<email>j.mankouri@leeds.ac.uk</email> (J.M.);<email>g.e.blair@leeds.ac.uk</email> (G.E.B.)</aff><author-notes><corresp id="c1-viruses-07-02780"><label>*</label>Author to whom correspondence should be addressed; E-Mail: <email>n.j.stonehouse@leeds.ac.uk</email>; Tel.: +44-113-343-3102.</corresp></author-notes><pub-date pub-type="epub"><day>26</day><month>6</month><year>2015</year></pub-date><pub-date pub-type="collection"><month>7</month><year>2015</year></pub-date><volume>7</volume><issue>7</issue><fpage>3443</fpage><lpage>3461</lpage><history><date date-type="received"><day>09</day><month>4</month><year>2015</year></date><date date-type="accepted"><day>17</day><month>6</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 by the authors; licensee MDPI, Basel, Switzerland.</copyright-statement><copyright-year>2015</copyright-year><license><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>).</license-p></license></permissions><abstract><p>Human papillomavirus (HPV) is the most common viral infection of the reproductive tract, affecting both men and women. High-risk oncogenic types are responsible for almost 90% of anogenital and oropharyngeal cancers including cervical cancer. Some of the HPV “early” genes, particularly E6 and E7, are known to act as oncogenes that promote tumour growth and malignant transformation. Most notably, HPV-16 E7 interacts with the tumour suppressor protein pRb, promoting its degradation, leading to cell cycle dysregulation in infected cells. We have previously shown that an RNA aptamer (termed A2) selectively binds to HPV16 E7 and is able to induce apoptosis in HPV16-transformed cervical carcinoma cell lines (SiHa) through reduction of E7 levels. In this study, we investigated the effects of the A2 aptamer on E7 localisation in order to define its effects on E7 activity. We demonstrate for the first time that E7 localised to the plasma membrane. In addition, we show that A2 enhanced E7 localisation in the ER and that the A2-mediated reduction of E7 was not associated with proteasomal degradation. These data suggest that A2 perturbs normal E7 trafficking through promoting E7 ER retention.</p></abstract><kwd-group><kwd>HPV16 E7</kwd><kwd>SELEX</kwd><kwd>RNA aptamers</kwd><kwd>cell surface localisation</kwd><kwd>ER retention</kwd></kwd-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Blair, George Eric" sort="Blair, George Eric" uniqKey="Blair G" first="George Eric" last="Blair">George Eric Blair</name><name sortKey="Cesur, Ozlem" sort="Cesur, Ozlem" uniqKey="Cesur O" first="Özlem" last="Cesur">Özlem Cesur</name><name sortKey="Groves, Helen" sort="Groves, Helen" uniqKey="Groves H" first="Helen" last="Groves">Helen Groves</name><name sortKey="Mankouri, Jamel" sort="Mankouri, Jamel" uniqKey="Mankouri J" first="Jamel" last="Mankouri">Jamel Mankouri</name><name sortKey="Nicol, Clare" sort="Nicol, Clare" uniqKey="Nicol C" first="Clare" last="Nicol">Clare Nicol</name><name sortKey="Stonehouse, Nicola J" sort="Stonehouse, Nicola J" uniqKey="Stonehouse N" first="Nicola J." last="Stonehouse">Nicola J. 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