Bacterial community transcription patterns during a marine phytoplankton bloom
Identifieur interne : 000611 ( Main/Merge ); précédent : 000610; suivant : 000612Bacterial community transcription patterns during a marine phytoplankton bloom
Auteurs : Johanna M. Rinta-Kanto [États-Unis] ; Shulei Sun [États-Unis] ; Shalabh Sharma [États-Unis] ; Ronald P. Kiene [États-Unis] ; Mary Ann Moran [États-Unis]Source :
- Environmental Microbiology [ 1462-2912 ] ; 2012-01.
Abstract
Bacterioplankton consume a large proportion of photosynthetically fixed carbon in the ocean and control its biogeochemical fate. We used an experimental metatranscriptomics approach to compare bacterial activities that route energy and nutrients during a phytoplankton bloom compared with non‐bloom conditions. mRNAs were sequenced from duplicate bloom and control microcosms 1 day after a phytoplankton biomass peak, and transcript copies per litre of seawater were calculated using an internal mRNA standard. Transcriptome analysis revealed a potential novel mechanism for enhanced efficiency during carbon‐limited growth, mediated through membrane‐bound pyrophosphatases [V‐type H(+)‐translocating; hppA]; bloom bacterioplankton participated less in this metabolic energy scavenging than non‐bloom bacterioplankton, with possible implications for differences in growth yields on organic substrates. Bloom bacterioplankton transcribed more copies of genes predicted to increase cell surface adhesiveness, mediated by changes in bacterial signalling molecules related to biofilm formation and motility; these may be important in microbial aggregate formation. Bloom bacterioplankton also transcribed more copies of genes for organic acid utilization, suggesting an increased importance of this compound class in the bioreactive organic matter released during phytoplankton blooms. Transcription patterns were surprisingly faithful within a taxon regardless of treatment, suggesting that phylogeny broadly predicts the ecological roles of bacterial groups across ‘boom’ and ‘bust’ environmental backgrounds.
Url:
DOI: 10.1111/j.1462-2920.2011.02602.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000700
- to stream Istex, to step Curation: 000700
- to stream Istex, to step Checkpoint: 000129
Links to Exploration step
ISTEX:E2841A5EC19CD5FE75F4C0385374EE232EF19DB5Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Bacterial community transcription patterns during a marine phytoplankton bloom</title><author><name sortKey="Rinta Anto, Johanna M" sort="Rinta Anto, Johanna M" uniqKey="Rinta Anto J" first="Johanna M." last="Rinta-Kanto">Johanna M. Rinta-Kanto</name></author><author><name sortKey="Sun, Shulei" sort="Sun, Shulei" uniqKey="Sun S" first="Shulei" last="Sun">Shulei Sun</name></author><author><name sortKey="Sharma, Shalabh" sort="Sharma, Shalabh" uniqKey="Sharma S" first="Shalabh" last="Sharma">Shalabh Sharma</name></author><author><name sortKey="Kiene, Ronald P" sort="Kiene, Ronald P" uniqKey="Kiene R" first="Ronald P." last="Kiene">Ronald P. Kiene</name></author><author><name sortKey="Moran, Mary Ann" sort="Moran, Mary Ann" uniqKey="Moran M" first="Mary Ann" last="Moran">Mary Ann Moran</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E2841A5EC19CD5FE75F4C0385374EE232EF19DB5</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1111/j.1462-2920.2011.02602.x</idno><idno type="url">https://api.istex.fr/document/E2841A5EC19CD5FE75F4C0385374EE232EF19DB5/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000700</idno><idno type="wicri:Area/Istex/Curation">000700</idno><idno type="wicri:Area/Istex/Checkpoint">000129</idno><idno type="wicri:doubleKey">1462-2912:2012:Rinta Anto J:bacterial:community:transcription</idno><idno type="wicri:Area/Main/Merge">000611</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Bacterial community transcription patterns during a marine phytoplankton bloom</title><author><name sortKey="Rinta Anto, Johanna M" sort="Rinta Anto, Johanna M" uniqKey="Rinta Anto J" first="Johanna M." last="Rinta-Kanto">Johanna M. Rinta-Kanto</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Marine Sciences, University of Georgia, Athens, GA 30602</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Sun, Shulei" sort="Sun, Shulei" uniqKey="Sun S" first="Shulei" last="Sun">Shulei Sun</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Marine Sciences, University of Georgia, Athens, GA 30602</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Sharma, Shalabh" sort="Sharma, Shalabh" uniqKey="Sharma S" first="Shalabh" last="Sharma">Shalabh Sharma</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Marine Sciences, University of Georgia, Athens, GA 30602</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Kiene, Ronald P" sort="Kiene, Ronald P" uniqKey="Kiene R" first="Ronald P." last="Kiene">Ronald P. Kiene</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Marine Sciences, University of South Alabama, Mobile, AL 36688</wicri:regionArea><placeName><region type="state">Alabama</region></placeName></affiliation></author><author><name sortKey="Moran, Mary Ann" sort="Moran, Mary Ann" uniqKey="Moran M" first="Mary Ann" last="Moran">Mary Ann Moran</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Marine Sciences, University of Georgia, Athens, GA 30602</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Environmental Microbiology</title><idno type="ISSN">1462-2912</idno><idno type="eISSN">1462-2920</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-01">2012-01</date><biblScope unit="volume">14</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="228">228</biblScope><biblScope unit="page" to="239">239</biblScope></imprint><idno type="ISSN">1462-2912</idno></series><idno type="istex">E2841A5EC19CD5FE75F4C0385374EE232EF19DB5</idno><idno type="DOI">10.1111/j.1462-2920.2011.02602.x</idno><idno type="ArticleID">EMI2602</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1462-2912</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bacterioplankton consume a large proportion of photosynthetically fixed carbon in the ocean and control its biogeochemical fate. We used an experimental metatranscriptomics approach to compare bacterial activities that route energy and nutrients during a phytoplankton bloom compared with non‐bloom conditions. mRNAs were sequenced from duplicate bloom and control microcosms 1 day after a phytoplankton biomass peak, and transcript copies per litre of seawater were calculated using an internal mRNA standard. Transcriptome analysis revealed a potential novel mechanism for enhanced efficiency during carbon‐limited growth, mediated through membrane‐bound pyrophosphatases [V‐type H(+)‐translocating; hppA]; bloom bacterioplankton participated less in this metabolic energy scavenging than non‐bloom bacterioplankton, with possible implications for differences in growth yields on organic substrates. Bloom bacterioplankton transcribed more copies of genes predicted to increase cell surface adhesiveness, mediated by changes in bacterial signalling molecules related to biofilm formation and motility; these may be important in microbial aggregate formation. Bloom bacterioplankton also transcribed more copies of genes for organic acid utilization, suggesting an increased importance of this compound class in the bioreactive organic matter released during phytoplankton blooms. Transcription patterns were surprisingly faithful within a taxon regardless of treatment, suggesting that phylogeny broadly predicts the ecological roles of bacterial groups across ‘boom’ and ‘bust’ environmental backgrounds.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/CyberinfraV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000611 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000611 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Ticri/CIDE
|area= CyberinfraV1
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:E2841A5EC19CD5FE75F4C0385374EE232EF19DB5
|texte= Bacterial community transcription patterns during a marine phytoplankton bloom
}}
| This area was generated with Dilib version V0.6.25. |  |