ZOOSPOROGENESIS, MORPHOLOGY, ULTRASTRUCTURE, PIGMENT COMPOSITION, AND PHYLOGENETIC POSITION OF TRACHYDISCUS MINUTUS (EUSTIGMATOPHYCEAE, HETEROKONTOPHYTA)
Identifieur interne : 000668 ( Istex/Corpus ); précédent : 000667; suivant : 000669ZOOSPOROGENESIS, MORPHOLOGY, ULTRASTRUCTURE, PIGMENT COMPOSITION, AND PHYLOGENETIC POSITION OF TRACHYDISCUS MINUTUS (EUSTIGMATOPHYCEAE, HETEROKONTOPHYTA)
Auteurs : Pavel P Ibyl ; Marek Eliáš ; Vladislav Cepák ; Jaromír Lukavsk ; Petr KaštánekSource :
- Journal of Phycology [ 0022-3646 ] ; 2012-02.
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- KwdEn :
Abstract
The traditional order Mischococcales (Xanthophyceae) is polyphyletic with some original members now classified in a separate class, Eustigmatophyceae. However, most mischococcalean species have not yet been studied in detail, raising the possibility that many of them still remain misplaced. We established an algal culture (strain CCALA 838) determined as one such species, Trachydiscus minutus (Bourr.) H. Ettl, and studied the morphology, ultrastructure, life cycle, pigment composition, and phylogeny using the 18S rRNA gene. We discovered a zoosporic part of the life cycle of this alga. Zoospore production was induced by darkness, suppressed by light, and was temperature dependent. The zoospores possessed one flagellum covered with mastigonemes and exhibited a basal swelling, but a stigma was missing. Ultrastructural investigations of vegetative cells revealed plastids lacking both a connection to the nuclear envelope and a girdle lamella. Moreover, we described biogenesis of oil bodies on the ultrastructural level. Photosynthetic pigments of T. minutus included as the major carotenoids violaxanthin and vaucheriaxanthin (ester); we detected no chl c. An 18S rRNA gene‐based phylogenetic analysis placed T. minutus in a clade with species of the genus Pseudostaurastrum and with Goniochloris sculpta Geitler, which form a sister branch to initially studied Eustigmatophyceae. In summary, our results are inconsistent with classifying T. minutus as a xanthophycean and indicate that it is a member of a novel deep lineage of the class Eustigmatophyceae.
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DOI: 10.1111/j.1529-8817.2011.01109.x
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ISTEX:D07109B691339312C795DAA9FEB91510DC744E69Le document en format XML
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Mischococcales (Xanthophyceae) is polyphyletic with some original members now classified in a separate class, Eustigmatophyceae. However, most mischococcalean species have not yet been studied in detail, raising the possibility that many of them still remain misplaced. We established an algal culture (strain CCALA 838) determined as one such species, Trachydiscus minutus (Bourr.) H. Ettl, and studied the morphology, ultrastructure, life cycle, pigment composition, and phylogeny using the 18S rRNA gene. We discovered a zoosporic part of the life cycle of this alga. Zoospore production was induced by darkness, suppressed by light, and was temperature dependent. The zoospores possessed one flagellum covered with mastigonemes and exhibited a basal swelling, but a stigma was missing. Ultrastructural investigations of vegetative cells revealed plastids lacking both a connection to the nuclear envelope and a girdle lamella. Moreover, we described biogenesis of oil bodies on the ultrastructural level. Photosynthetic pigments of T. minutus included as the major carotenoids violaxanthin and vaucheriaxanthin (ester); we detected no chl c. An 18S rRNA gene‐based phylogenetic analysis placed T. minutus in a clade with species of the genus Pseudostaurastrum and with Goniochloris sculpta Geitler, which form a sister branch to initially studied Eustigmatophyceae. 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Photosynthetic pigments of T. minutus included as the major carotenoids violaxanthin and vaucheriaxanthin (ester); we detected no chl c. An 18S rRNA gene‐based phylogenetic analysis placed T. minutus in a clade with species of the genus Pseudostaurastrum and with Goniochloris sculpta Geitler, which form a sister branch to initially studied Eustigmatophyceae. In summary, our results are inconsistent with classifying T. minutus as a xanthophycean and indicate that it is a member of a novel deep lineage of the class Eustigmatophyceae.</abstract><qualityIndicators><score>7.712</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 782.362 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>9</keywordCount><abstractCharCount>1568</abstractCharCount><pdfWordCount>7500</pdfWordCount><pdfCharCount>47427</pdfCharCount><pdfPageCount>12</pdfPageCount><abstractWordCount>226</abstractWordCount></qualityIndicators><title>ZOOSPOROGENESIS, MORPHOLOGY, ULTRASTRUCTURE, PIGMENT COMPOSITION, AND PHYLOGENETIC POSITION OF TRACHYDISCUS MINUTUS (EUSTIGMATOPHYCEAE, HETEROKONTOPHYTA)</title><genre><json:string>article</json:string></genre><host><volume>48</volume><publisherId><json:string>JPY</json:string></publisherId><pages><total>15</total><last>242</last><first>231</first></pages><issn><json:string>0022-3646</json:string></issn><issue>1</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1529-8817</json:string></eissn><title>Journal of Phycology</title><doi><json:string>10.1111/(ISSN)1529-8817</json:string></doi></host><publicationDate>2012</publicationDate><copyrightDate>2012</copyrightDate><doi><json:string>10.1111/j.1529-8817.2011.01109.x</json:string></doi><id>D07109B691339312C795DAA9FEB91510DC744E69</id><score>0.100814484</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/D07109B691339312C795DAA9FEB91510DC744E69/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/D07109B691339312C795DAA9FEB91510DC744E69/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/D07109B691339312C795DAA9FEB91510DC744E69/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">ZOOSPOROGENESIS, MORPHOLOGY, ULTRASTRUCTURE, PIGMENT COMPOSITION, AND PHYLOGENETIC POSITION OF TRACHYDISCUS MINUTUS (EUSTIGMATOPHYCEAE, HETEROKONTOPHYTA)</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>© 2011 Phycological Society of America</p></availability><date>2012</date></publicationStmt><notesStmt><note type="content">*Received 8 October 2010. Accepted 28 July 2011.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">ZOOSPOROGENESIS, MORPHOLOGY, ULTRASTRUCTURE, PIGMENT COMPOSITION, AND PHYLOGENETIC POSITION OF TRACHYDISCUS MINUTUS (EUSTIGMATOPHYCEAE, HETEROKONTOPHYTA)</title><author xml:id="author-1"><persName><forename type="first">Pavel</forename><surname>Přibyl</surname></persName><affiliation>Author for correspondence: e‐mail pribyl@butbn.cas.cz.</affiliation><affiliation>Algological Centre and Centre for Bioindication and Revitalisation, Institute of Botany, v.v.i., Academy of Sciences of the Czech Republic, Dukelská 135, Třeboň CZ‐379 82, Czech Republic</affiliation></author><author xml:id="author-2"><persName><forename type="first">Marek</forename><surname>Eliáš</surname></persName><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, Prague CZ‐128 01, Czech Republic Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, Slezská Ostrava CZ‐710 00, Czech Republic</affiliation></author><author xml:id="author-3"><persName><forename type="first">Vladislav</forename><surname>Cepák</surname></persName><affiliation>Algological Centre and Centre for Bioindication and Revitalisation, Institute of Botany, v.v.i., Academy of Sciences of the Czech Republic, Dukelská 135, Třeboň CZ‐379 82, Czech Republic</affiliation></author><author xml:id="author-4"><persName><forename type="first">Jaromír</forename><surname>Lukavský</surname></persName><affiliation>Algological Centre and Centre for Bioindication and Revitalisation, Institute of Botany, v.v.i., Academy of Sciences of the Czech Republic, Dukelská 135, Třeboň CZ‐379 82, Czech Republic</affiliation></author><author xml:id="author-5"><persName><forename type="first">Petr</forename><surname>Kaštánek</surname></persName><affiliation>EcoFuel Laboratories Ltd., Sázavská 17, Prague CZ‐120 00, Czech Republic</affiliation></author></analytic><monogr><title level="j">Journal of Phycology</title><idno type="pISSN">0022-3646</idno><idno type="eISSN">1529-8817</idno><idno type="DOI">10.1111/(ISSN)1529-8817</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-02"></date><biblScope unit="volume">48</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="231">231</biblScope><biblScope unit="page" to="242">242</biblScope></imprint></monogr><idno type="istex">D07109B691339312C795DAA9FEB91510DC744E69</idno><idno type="DOI">10.1111/j.1529-8817.2011.01109.x</idno><idno type="ArticleID">JPY1109</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The traditional order Mischococcales (Xanthophyceae) is polyphyletic with some original members now classified in a separate class, Eustigmatophyceae. However, most mischococcalean species have not yet been studied in detail, raising the possibility that many of them still remain misplaced. We established an algal culture (strain CCALA 838) determined as one such species, Trachydiscus minutus (Bourr.) H. Ettl, and studied the morphology, ultrastructure, life cycle, pigment composition, and phylogeny using the 18S rRNA gene. We discovered a zoosporic part of the life cycle of this alga. Zoospore production was induced by darkness, suppressed by light, and was temperature dependent. The zoospores possessed one flagellum covered with mastigonemes and exhibited a basal swelling, but a stigma was missing. Ultrastructural investigations of vegetative cells revealed plastids lacking both a connection to the nuclear envelope and a girdle lamella. Moreover, we described biogenesis of oil bodies on the ultrastructural level. Photosynthetic pigments of T. minutus included as the major carotenoids violaxanthin and vaucheriaxanthin (ester); we detected no chl c. An 18S rRNA gene‐based phylogenetic analysis placed T. minutus in a clade with species of the genus Pseudostaurastrum and with Goniochloris sculpta Geitler, which form a sister branch to initially studied Eustigmatophyceae. In summary, our results are inconsistent with classifying T. minutus as a xanthophycean and indicate that it is a member of a novel deep lineage of the class Eustigmatophyceae.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>18S rRNA</term></item><item><term>autofluorescence</term></item><item><term>Goniochloris sculpta</term></item><item><term>lipid bodies</term></item><item><term>photosynthetic pigments</term></item><item><term>phylogeny</term></item><item><term>Pseudostaurastrum</term></item><item><term>Trachydiscus minutus</term></item><item><term>zoospores</term></item></list></keywords></textClass></profileDesc><revisionDesc><change 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MORPHOLOGY, ULTRASTRUCTURE, PIGMENT COMPOSITION, AND PHYLOGENETIC POSITION OF <i>TRACHYDISCUS MINUTUS</i> (EUSTIGMATOPHYCEAE, HETEROKONTOPHYTA)</title><title type="shortAuthors">PAVEL PŘIBYL ET AL.</title><title type="short"><i>TRACHYDISCUS MINUTUS</i></title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" noteRef="#fn2"><personName><givenNames>Pavel</givenNames><familyName>Přibyl</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>Marek</givenNames><familyName>Eliáš</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>Vladislav</givenNames><familyName>Cepák</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>Jaromír</givenNames><familyName>Lukavský</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a4"><personName><givenNames>Petr</givenNames><familyName>Kaštánek</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CZ"><unparsedAffiliation>Algological Centre and Centre for Bioindication and Revitalisation, Institute of Botany, v.v.i., Academy of Sciences of the Czech Republic, Dukelská 135, Třeboň CZ‐379 82, Czech Republic</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="CZ"><unparsedAffiliation> Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, Prague CZ‐128 01, Czech Republic
Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, Slezská Ostrava CZ‐710 00, Czech Republic</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="CZ"><unparsedAffiliation>Algological Centre and Centre for Bioindication and Revitalisation, Institute of Botany, v.v.i., Academy of Sciences of the Czech Republic, Dukelská 135, Třeboň CZ‐379 82, Czech Republic</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="CZ"><unparsedAffiliation>EcoFuel Laboratories Ltd., Sázavská 17, Prague CZ‐120 00, Czech Republic</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">18S rRNA</keyword><keyword xml:id="k2">autofluorescence</keyword><keyword xml:id="k3"><i>Goniochloris sculpta</i></keyword><keyword xml:id="k4">lipid bodies</keyword><keyword xml:id="k5">photosynthetic pigments</keyword><keyword xml:id="k6">phylogeny</keyword><keyword xml:id="k7"><i>Pseudostaurastrum</i></keyword><keyword xml:id="k8"><i>Trachydiscus minutus</i></keyword><keyword xml:id="k9">zoospores</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The traditional order Mischococcales (Xanthophyceae) is polyphyletic with some original members now classified in a separate class, Eustigmatophyceae. However, most mischococcalean species have not yet been studied in detail, raising the possibility that many of them still remain misplaced. We established an algal culture (strain CCALA 838) determined as one such species, <i>Trachydiscus minutus</i> (Bourr.) H. Ettl, and studied the morphology, ultrastructure, life cycle, pigment composition, and phylogeny using the 18S rRNA gene. We discovered a zoosporic part of the life cycle of this alga. Zoospore production was induced by darkness, suppressed by light, and was temperature dependent. The zoospores possessed one flagellum covered with mastigonemes and exhibited a basal swelling, but a stigma was missing. Ultrastructural investigations of vegetative cells revealed plastids lacking both a connection to the nuclear envelope and a girdle lamella. Moreover, we described biogenesis of oil bodies on the ultrastructural level. Photosynthetic pigments of <i>T</i>. <i>minutus</i> included as the major carotenoids violaxanthin and vaucheriaxanthin (ester); we detected no chl <i>c</i>. An 18S rRNA gene‐based phylogenetic analysis placed <i>T. minutus</i> in a clade with species of the genus <i>Pseudostaurastrum</i> and with <i>Goniochloris sculpta</i> Geitler, which form a sister branch to initially studied Eustigmatophyceae. In summary, our results are inconsistent with classifying <i>T. minutus</i> as a xanthophycean and indicate that it is a member of a novel deep lineage of the class Eustigmatophyceae.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p> Received 8 October 2010. Accepted 28 July 2011.</p></note><note xml:id="fn2"><p> Author for correspondence: e‐mail <email>pribyl@butbn.cas.cz</email>.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>ZOOSPOROGENESIS, MORPHOLOGY, ULTRASTRUCTURE, PIGMENT COMPOSITION, AND PHYLOGENETIC POSITION OF TRACHYDISCUS MINUTUS (EUSTIGMATOPHYCEAE, HETEROKONTOPHYTA)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>TRACHYDISCUS MINUTUS</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>ZOOSPOROGENESIS, MORPHOLOGY, ULTRASTRUCTURE, PIGMENT COMPOSITION, AND PHYLOGENETIC POSITION OF TRACHYDISCUS MINUTUS (EUSTIGMATOPHYCEAE, HETEROKONTOPHYTA)1</title></titleInfo><name type="personal"><namePart type="given">Pavel</namePart><namePart type="family">Přibyl</namePart><affiliation>Algological Centre and Centre for Bioindication and Revitalisation, Institute of Botany, v.v.i., Academy of Sciences of the Czech Republic, Dukelská 135, Třeboň CZ‐379 82, Czech Republic</affiliation><description>Author for correspondence: e‐mail pribyl@butbn.cas.cz.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marek</namePart><namePart type="family">Eliáš</namePart><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, Prague CZ‐128 01, Czech Republic Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, Slezská Ostrava CZ‐710 00, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Vladislav</namePart><namePart type="family">Cepák</namePart><affiliation>Algological Centre and Centre for Bioindication and Revitalisation, Institute of Botany, v.v.i., Academy of Sciences of the Czech Republic, Dukelská 135, Třeboň CZ‐379 82, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jaromír</namePart><namePart type="family">Lukavský</namePart><affiliation>Algological Centre and Centre for Bioindication and Revitalisation, Institute of Botany, v.v.i., Academy of Sciences of the Czech Republic, Dukelská 135, Třeboň CZ‐379 82, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Petr</namePart><namePart type="family">Kaštánek</namePart><affiliation>EcoFuel Laboratories Ltd., Sázavská 17, Prague CZ‐120 00, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2012-02</dateIssued><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">9</extent><extent unit="tables">1</extent></physicalDescription><abstract lang="en">The traditional order Mischococcales (Xanthophyceae) is polyphyletic with some original members now classified in a separate class, Eustigmatophyceae. However, most mischococcalean species have not yet been studied in detail, raising the possibility that many of them still remain misplaced. We established an algal culture (strain CCALA 838) determined as one such species, Trachydiscus minutus (Bourr.) H. Ettl, and studied the morphology, ultrastructure, life cycle, pigment composition, and phylogeny using the 18S rRNA gene. We discovered a zoosporic part of the life cycle of this alga. Zoospore production was induced by darkness, suppressed by light, and was temperature dependent. The zoospores possessed one flagellum covered with mastigonemes and exhibited a basal swelling, but a stigma was missing. Ultrastructural investigations of vegetative cells revealed plastids lacking both a connection to the nuclear envelope and a girdle lamella. Moreover, we described biogenesis of oil bodies on the ultrastructural level. Photosynthetic pigments of T. minutus included as the major carotenoids violaxanthin and vaucheriaxanthin (ester); we detected no chl c. An 18S rRNA gene‐based phylogenetic analysis placed T. minutus in a clade with species of the genus Pseudostaurastrum and with Goniochloris sculpta Geitler, which form a sister branch to initially studied Eustigmatophyceae. In summary, our results are inconsistent with classifying T. minutus as a xanthophycean and indicate that it is a member of a novel deep lineage of the class Eustigmatophyceae.</abstract><note type="content">*Received 8 October 2010. Accepted 28 July 2011.</note><subject lang="en"><genre>keywords</genre><topic>18S rRNA</topic><topic>autofluorescence</topic><topic>Goniochloris sculpta</topic><topic>lipid bodies</topic><topic>photosynthetic pigments</topic><topic>phylogeny</topic><topic>Pseudostaurastrum</topic><topic>Trachydiscus minutus</topic><topic>zoospores</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Phycology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-3646</identifier><identifier type="eISSN">1529-8817</identifier><identifier type="DOI">10.1111/(ISSN)1529-8817</identifier><identifier type="PublisherID">JPY</identifier><part><date>2012</date><detail type="volume"><caption>vol.</caption><number>48</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>231</start><end>242</end><total>15</total></extent></part></relatedItem><identifier type="istex">D07109B691339312C795DAA9FEB91510DC744E69</identifier><identifier type="DOI">10.1111/j.1529-8817.2011.01109.x</identifier><identifier type="ArticleID">JPY1109</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 Phycological Society of America</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/D07109B691339312C795DAA9FEB91510DC744E69/enrichments/multicat</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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