Expression of inducible nitric oxide synthase in thyroid neoplasms: Immunohistochemical and molecular analysis
Identifieur interne : 003D13 ( Istex/Corpus ); précédent : 003D12; suivant : 003D14Expression of inducible nitric oxide synthase in thyroid neoplasms: Immunohistochemical and molecular analysis
Auteurs : Wonsick Choe ; Sungeun Kim ; Tae Sook Hwang ; Seung Sook LeeSource :
- Pathology International [ 1320-5463 ] ; 2003-07.
English descriptors
- Teeft :
- Adenoma, Anaplastic carcinomas, Carcinoma, Chain reaction, Diffuse immunostaining, Follicular, Follicular adenomas, Follicular carcinomas, Follicular epithelial origin, Follicular neoplasms, Immunohistochemical, Immunohistochemical staining, Immunostaining, Inducible, Inducible nitric oxide synthase, Inos, Inos expression, Medullary, Medullary carcinomas, Neoplasm, Neoplastic cells, Nitric, Nitric oxide, Nitric oxide synthase, Nitric oxide synthase activity, Normal thyroid, Oxide, Papillary, Papillary carcinomas, Present study, Room temperature, Surgical specimens, Synthase, Thyroid carcinomas, Thyroid neoplasm, Thyroid neoplasms, Tumor cells, Western blot analysis.
Abstract
To understand the role of inducible nitric oxide synthase (iNOS) in thyroid tumorigenesis, immunohistochemical staining of 36 surgical specimens of thyroid neoplasm that consisted of seven follicular adenomas, 12 papillary carcinomas, seven follicular carcinomas, five medullary carcinomas, and five anaplastic carcinomas were analyzed. In addition, 20 specimens of normal thyroid were used as control samples. Reverse transcription–polymerase chain reaction and western blot analysis were also performed using a normal thyroid and a representative papillary carcinoma case. The intensity and proportion of the immunostained tumor cells were graded semiquantitatively. The grades of the intensity and the proportion were then summed to provide an immunohistochemical score. There was a variation in the staining intensity and proportion. The iNOS expression was low in normal follicular epithelia. Inducible nitric oxide synthase is present in the majority of thyroid tumor cells, including follicular adenomas, papillary carcinomas, follicular carcinomas, medullary carcinomas, and anaplastic carcinomas. Relatively low expression was shown in follicular neoplasms. Only a few inflammatory cells in the stroma were immunoreactive. These results suggest that iNOS may have a role in tumorigenesis, and iNOS in human thyroid carcinoma is mostly derived from tumor cells not from macrophages.
Url:
DOI: 10.1046/j.1440-1827.2003.01501.x
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ISTEX:EB662FECDD859FA295BDC747DDE406A4AB55991CLe document en format XML
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In addition, 20 specimens of normal thyroid were used as control samples. Reverse transcription–polymerase chain reaction and western blot analysis were also performed using a normal thyroid and a representative papillary carcinoma case. The intensity and proportion of the immunostained tumor cells were graded semiquantitatively. The grades of the intensity and the proportion were then summed to provide an immunohistochemical score. There was a variation in the staining intensity and proportion. The iNOS expression was low in normal follicular epithelia. Inducible nitric oxide synthase is present in the majority of thyroid tumor cells, including follicular adenomas, papillary carcinomas, follicular carcinomas, medullary carcinomas, and anaplastic carcinomas. Relatively low expression was shown in follicular neoplasms. Only a few inflammatory cells in the stroma were immunoreactive. 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</orgName></affiliation></author><author xml:id="author-0002" role="corresp"><persName><forename type="first">Tae Sook</forename><surname>Hwang</surname></persName><affiliation><address><addrLine>Pathology</addrLine><addrLine>Inha University College of Medicine</addrLine><addrLine>Incheon and
</addrLine></address></affiliation><affiliation>Tae Sook Hwang, MD, Department of Pathology, Inha University College of Medicine, 7‐241, 3rd st. Shinheung‐dong, Choong‐gu, Incheon 400‐103, Korea. Email: tshwang@inha.ac.kr</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Seung Sook</forename><surname>Lee</surname></persName><affiliation><orgName type="department">Department of Pathology</orgName><address><addrLine>Korea Cancer Center Hospital</addrLine><addrLine>Seoul</addrLine><country key="KR">KOREA, REPUBLIC OF</country><country key="KR"></country></address></affiliation></author><idno type="istex">EB662FECDD859FA295BDC747DDE406A4AB55991C</idno><idno type="ark">ark:/67375/WNG-CSB9VJ27-K</idno><idno type="DOI">10.1046/j.1440-1827.2003.01501.x</idno><idno type="unit">PIN1501</idno><idno type="toTypesetVersion">file:PIN.PIN1501.pdf</idno></analytic><monogr><title level="j" type="main">Pathology International</title><title level="j" type="alt">PATHOLOGY INTERNATIONAL</title><idno type="pISSN">1320-5463</idno><idno type="eISSN">1440-1827</idno><idno type="book-DOI">10.1111/(ISSN)1440-1827</idno><idno type="book-part-DOI">10.1111/pin.2003.53.issue-7</idno><idno type="product">PIN</idno><idno type="publisherDivision">ST</idno><imprint><biblScope unit="vol">53</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="434">434</biblScope><biblScope unit="page" to="439">439</biblScope><biblScope unit="page-count">6</biblScope><publisher>Blackwell Science Pty</publisher><pubPlace>Melbourne, Australia</pubPlace><date type="published" when="2003-07"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main"><p>To understand the role of inducible nitric oxide synthase (iNOS) in thyroid tumorigenesis, immunohistochemical staining of 36 surgical specimens of thyroid neoplasm that consisted of seven follicular adenomas, 12 papillary carcinomas, seven follicular carcinomas, five medullary carcinomas, and five anaplastic carcinomas were analyzed. In addition, 20 specimens of normal thyroid were used as control samples. Reverse transcription–polymerase chain reaction and western blot analysis were also performed using a normal thyroid and a representative papillary carcinoma case. The intensity and proportion of the immunostained tumor cells were graded semiquantitatively. The grades of the intensity and the proportion were then summed to provide an immunohistochemical score. There was a variation in the staining intensity and proportion. The iNOS expression was low in normal follicular epithelia. Inducible nitric oxide synthase is present in the majority of thyroid tumor cells, including follicular adenomas, papillary carcinomas, follicular carcinomas, medullary carcinomas, and anaplastic carcinomas. Relatively low expression was shown in follicular neoplasms. Only a few inflammatory cells in the stroma were immunoreactive. These results suggest that iNOS may have a role in tumorigenesis, and iNOS in human thyroid carcinoma is mostly derived from tumor cells not from macrophages.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="k1">immunohistochemistry</term><term xml:id="k2">inducible nitric oxide synthase</term><term xml:id="k3">thyroid neoplasm </term></keywords><keywords rend="tocHeading1"><term>Original Articles</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-CSB9VJ27-K/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Science Pty</publisherName><publisherLoc>Melbourne, Australia</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1440-1827</doi><issn type="print">1320-5463</issn><issn type="electronic">1440-1827</issn><idGroup><id type="product" value="PIN"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="PATHOLOGY INTERNATIONAL">Pathology International</title></titleGroup></publicationMeta><publicationMeta level="part" position="07007"><doi origin="wiley">10.1111/pin.2003.53.issue-7</doi><numberingGroup><numbering type="journalVolume" number="53">53</numbering><numbering type="journalIssue" number="7">7</numbering></numberingGroup><coverDate startDate="2003-07">July 2003</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0043400" status="forIssue"><doi origin="wiley">10.1046/j.1440-1827.2003.01501.x</doi><idGroup><id type="unit" value="PIN1501"></id></idGroup><countGroup><count type="pageTotal" number="6"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><eventGroup><event type="firstOnline" date="2003-07-04"></event><event type="publishedOnlineFinalForm" date="2003-07-04"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.4 mode:FullText source:FullText result:FullText" date="2010-03-30"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-11-03"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="434">434</numbering><numbering type="pageLast" number="439">439</numbering></numberingGroup><correspondenceTo> Tae Sook Hwang, MD, Department of Pathology, Inha University College of Medicine, 7‐241, 3rd st. Shinheung‐dong, Choong‐gu, Incheon 400‐103, Korea. Email: <email>tshwang@inha.ac.kr</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:PIN.PIN1501.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 17 October 2002. Accepted for publication 28 February 2003.</unparsedEditorialHistory><countGroup><count type="figureTotal" number="0"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="23"></count><count type="wordTotal" number="2363"></count><count type="linksPubMed" number="22"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Expression of inducible nitric oxide synthase in thyroid neoplasms: Immunohistochemical and molecular analysis</title><title type="shortAuthors">W. Choe <i>et al</i>.</title><title type="short">Inducible NO synthase in thyroid neoplasm</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Wonsick</givenNames><familyName>Choe</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>Sungeun</givenNames><familyName>Kim</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a2" corresponding="yes"><personName><givenNames>Tae Sook</givenNames><familyName>Hwang</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>Seung Sook</givenNames><familyName>Lee</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1"><unparsedAffiliation>Departments of Nuclear Medicine and
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</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="KR"><unparsedAffiliation>Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">immunohistochemistry</keyword><keyword xml:id="k2">inducible nitric oxide synthase</keyword><keyword xml:id="k3">thyroid neoplasm </keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>To understand the role of inducible nitric oxide synthase (iNOS) in thyroid tumorigenesis, immunohistochemical staining of 36 surgical specimens of thyroid neoplasm that consisted of seven follicular adenomas, 12 papillary carcinomas, seven follicular carcinomas, five medullary carcinomas, and five anaplastic carcinomas were analyzed. In addition, 20 specimens of normal thyroid were used as control samples. Reverse transcription–polymerase chain reaction and western blot analysis were also performed using a normal thyroid and a representative papillary carcinoma case. The intensity and proportion of the immunostained tumor cells were graded semiquantitatively. The grades of the intensity and the proportion were then summed to provide an immunohistochemical score. There was a variation in the staining intensity and proportion. The iNOS expression was low in normal follicular epithelia. Inducible nitric oxide synthase is present in the majority of thyroid tumor cells, including follicular adenomas, papillary carcinomas, follicular carcinomas, medullary carcinomas, and anaplastic carcinomas. Relatively low expression was shown in follicular neoplasms. Only a few inflammatory cells in the stroma were immunoreactive. These results suggest that iNOS may have a role in tumorigenesis, and iNOS in human thyroid carcinoma is mostly derived from tumor cells not from macrophages.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Expression of inducible nitric oxide synthase in thyroid neoplasms: Immunohistochemical and molecular analysis</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Inducible NO synthase in thyroid neoplasm</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Expression of inducible nitric oxide synthase in thyroid neoplasms: Immunohistochemical and molecular analysis</title></titleInfo><name type="personal"><namePart type="given">Wonsick</namePart><namePart type="family">Choe</namePart><affiliation>Departments of Nuclear Medicine and</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sungeun</namePart><namePart type="family">Kim</namePart><affiliation>Departments of Nuclear Medicine and</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tae Sook</namePart><namePart type="family">Hwang</namePart><affiliation>Pathology, Inha University College of Medicine, Incheon and</affiliation><affiliation>E-mail: tshwang@inha.ac.kr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Seung Sook</namePart><namePart type="family">Lee</namePart><affiliation>Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Science Pty</publisher><place><placeTerm type="text">Melbourne, Australia</placeTerm></place><dateIssued encoding="w3cdtf">2003-07</dateIssued><edition>Received 17 October 2002. Accepted for publication 28 February 2003.</edition><copyrightDate encoding="w3cdtf">2003</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">0</extent><extent unit="tables">0</extent><extent unit="formulas">0</extent><extent unit="references">23</extent><extent unit="linksCrossRef">0</extent><extent unit="words">2363</extent></physicalDescription><abstract lang="en">To understand the role of inducible nitric oxide synthase (iNOS) in thyroid tumorigenesis, immunohistochemical staining of 36 surgical specimens of thyroid neoplasm that consisted of seven follicular adenomas, 12 papillary carcinomas, seven follicular carcinomas, five medullary carcinomas, and five anaplastic carcinomas were analyzed. In addition, 20 specimens of normal thyroid were used as control samples. Reverse transcription–polymerase chain reaction and western blot analysis were also performed using a normal thyroid and a representative papillary carcinoma case. The intensity and proportion of the immunostained tumor cells were graded semiquantitatively. The grades of the intensity and the proportion were then summed to provide an immunohistochemical score. There was a variation in the staining intensity and proportion. The iNOS expression was low in normal follicular epithelia. Inducible nitric oxide synthase is present in the majority of thyroid tumor cells, including follicular adenomas, papillary carcinomas, follicular carcinomas, medullary carcinomas, and anaplastic carcinomas. Relatively low expression was shown in follicular neoplasms. Only a few inflammatory cells in the stroma were immunoreactive. These results suggest that iNOS may have a role in tumorigenesis, and iNOS in human thyroid carcinoma is mostly derived from tumor cells not from macrophages.</abstract><subject lang="en"><genre>keywords</genre><topic>immunohistochemistry</topic><topic>inducible nitric oxide synthase</topic><topic>thyroid neoplasm</topic></subject><relatedItem type="host"><titleInfo><title>Pathology International</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">1320-5463</identifier><identifier type="eISSN">1440-1827</identifier><identifier type="DOI">10.1111/(ISSN)1440-1827</identifier><identifier type="PublisherID">PIN</identifier><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>53</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>434</start><end>439</end><total>6</total></extent></part></relatedItem><identifier type="istex">EB662FECDD859FA295BDC747DDE406A4AB55991C</identifier><identifier type="ark">ark:/67375/WNG-CSB9VJ27-K</identifier><identifier type="DOI">10.1046/j.1440-1827.2003.01501.x</identifier><identifier type="ArticleID">PIN1501</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Blackwell Science Pty</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-CSB9VJ27-K/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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