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Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2‐ethylpyridine column

Identifieur interne : 001377 ( Main/Exploration ); précédent : 001376; suivant : 001378

Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2‐ethylpyridine column

Auteurs : Claudio Brunelli ; Yining Zhao [États-Unis] ; Melissa-Hanna Brown [Royaume-Uni] ; Pat Sandra

Source :

Journal of Separation Science [ 1615-9306 ] ; 2008-05.

RBID : ISTEX:FF742E545C9AC68FAE375B66A5A74E68847D5114

Descripteurs français

Pascal (Inist)
- Analyse chimique, Chromatographie SFC, Composition phase, Composition phase mobile, Médicament, Optimisation, Phase mobile, Phase stationnaire, Pyridine(2-éthyl).
Wicri :
- topic : Médicament.

English descriptors

KwdEn :
- 2‐Ethylpyridine stationary phase, Chemical analysis, Chemistry Techniques, Analytical (methods), Chemistry, Pharmaceutical (methods), Chromatography, Supercritical Fluid (methods), Clinical Laboratory Techniques, Drug, Formates (chemistry), Methanol (chemistry), Mobile phase, Mobile phase optimization, Models, Chemical, Optimization, Pharmaceutical Preparations (chemistry), Pharmaceuticals, Phase composition, Propylamines (analysis), Propylamines (chemistry), Pyridines (chemistry), Stationary phase, Steroids (chemistry), Supercritical fluid chromatography, Technology, Pharmaceutical (methods).
MESH :
- chemical , analysis : Propylamines.
- chemical , chemistry : Formates, Methanol, Pharmaceutical Preparations, Propylamines, Pyridines, Steroids.
- methods : Chemistry Techniques, Analytical, Chemistry, Pharmaceutical, Chromatography, Supercritical Fluid, Technology, Pharmaceutical.
- Clinical Laboratory Techniques, Models, Chemical.

Abstract

The separation of neutral, acidic, and basic pharmaceuticals with diverse physicochemical properties by packed column supercritical fluid chromatography (pSFC) on a 2‐ethylpyridine column (25 cm×4.6 mm id, 3 μm particles) is presented. The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open‐access generic screening environments.

Url:

https://api.istex.fr/document/FF742E545C9AC68FAE375B66A5A74E68847D5114/fulltext/pdf

DOI: 10.1002/jssc.200700555

Affiliations:

Le document en format XML

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<author><name sortKey="Brown, Melissa Anna" sort="Brown, Melissa Anna" uniqKey="Brown M" first="Melissa-Hanna" last="Brown">Melissa-Hanna Brown</name>
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<front><div type="abstract" xml:lang="en">The separation of neutral, acidic, and basic pharmaceuticals with diverse physicochemical properties by packed column supercritical fluid chromatography (pSFC) on a 2‐ethylpyridine column (25 cm×4.6 mm id, 3 μm particles) is presented. The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open‐access generic screening environments.</div>
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Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2‐ethylpyridine column

Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2‐ethylpyridine column

Source :

Descripteurs français

English descriptors

Abstract

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Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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