Comparison of the three optical platforms for measurement of cellular respiration.
Identifieur interne : 000012 ( PubMed/Corpus ); précédent : 000011; suivant : 000013Comparison of the three optical platforms for measurement of cellular respiration.
Auteurs : Alina V. Kondrashina ; Vladimir I. Ogurtsov ; Dmitri B. PapkovskySource :
- Analytical biochemistry [ 1096-0309 ] ; 2014.
Abstract
We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.
DOI: 10.1016/j.ab.2014.09.001
PubMed: 25233002
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of the three optical platforms for measurement of cellular respiration.</title><author><name sortKey="Kondrashina, Alina V" sort="Kondrashina, Alina V" uniqKey="Kondrashina A" first="Alina V" last="Kondrashina">Alina V. Kondrashina</name><affiliation><nlm:affiliation>School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.</nlm:affiliation></affiliation></author><author><name sortKey="Ogurtsov, Vladimir I" sort="Ogurtsov, Vladimir I" uniqKey="Ogurtsov V" first="Vladimir I" last="Ogurtsov">Vladimir I. Ogurtsov</name><affiliation><nlm:affiliation>Tyndall National Institute, Dyke Parade, Cork, Ireland.</nlm:affiliation></affiliation></author><author><name sortKey="Papkovsky, Dmitri B" sort="Papkovsky, Dmitri B" uniqKey="Papkovsky D" first="Dmitri B" last="Papkovsky">Dmitri B. Papkovsky</name><affiliation><nlm:affiliation>School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland. Electronic address: d.papkovsky@ucc.ie.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25233002</idno><idno type="pmid">25233002</idno><idno type="doi">10.1016/j.ab.2014.09.001</idno><idno type="wicri:Area/PubMed/Corpus">000012</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparison of the three optical platforms for measurement of cellular respiration.</title><author><name sortKey="Kondrashina, Alina V" sort="Kondrashina, Alina V" uniqKey="Kondrashina A" first="Alina V" last="Kondrashina">Alina V. Kondrashina</name><affiliation><nlm:affiliation>School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.</nlm:affiliation></affiliation></author><author><name sortKey="Ogurtsov, Vladimir I" sort="Ogurtsov, Vladimir I" uniqKey="Ogurtsov V" first="Vladimir I" last="Ogurtsov">Vladimir I. Ogurtsov</name><affiliation><nlm:affiliation>Tyndall National Institute, Dyke Parade, Cork, Ireland.</nlm:affiliation></affiliation></author><author><name sortKey="Papkovsky, Dmitri B" sort="Papkovsky, Dmitri B" uniqKey="Papkovsky D" first="Dmitri B" last="Papkovsky">Dmitri B. Papkovsky</name><affiliation><nlm:affiliation>School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland. Electronic address: d.papkovsky@ucc.ie.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Analytical biochemistry</title><idno type="eISSN">1096-0309</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">25233002</PMID><DateCreated><Year>2014</Year><Month>12</Month><Day>8</Day></DateCreated><DateRevised><Year>2014</Year><Month>12</Month><Day>8</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0309</ISSN><JournalIssue CitedMedium="Internet"><Volume>468C</Volume><PubDate><Year>2014</Year><Month>Sep</Month><Day>16</Day></PubDate></JournalIssue><Title>Analytical biochemistry</Title><ISOAbbreviation>Anal. Biochem.</ISOAbbreviation></Journal><ArticleTitle>Comparison of the three optical platforms for measurement of cellular respiration.</ArticleTitle><Pagination><MedlinePgn>1-3</MedlinePgn></Pagination><ELocationID EIdType="pii">S0003-2697(14)00399-6</ELocationID><ELocationID EIdType="doi">10.1016/j.ab.2014.09.001</ELocationID><Abstract><AbstractText>We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList><Author><LastName>Kondrashina</LastName><ForeName>Alina V</ForeName><Initials>AV</Initials><AffiliationInfo><Affiliation>School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.</Affiliation></AffiliationInfo></Author><Author><LastName>Ogurtsov</LastName><ForeName>Vladimir I</ForeName><Initials>VI</Initials><AffiliationInfo><Affiliation>Tyndall National Institute, Dyke Parade, Cork, Ireland.</Affiliation></AffiliationInfo></Author><Author><LastName>Papkovsky</LastName><ForeName>Dmitri B</ForeName><Initials>DB</Initials><AffiliationInfo><Affiliation>School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland. Electronic address: d.papkovsky@ucc.ie.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="">JOURNAL ARTICLE</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>9</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><MedlineTA>Anal Biochem</MedlineTA><NlmUniqueID>0370535</NlmUniqueID><ISSNLinking>0003-2697</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cell oxygenation</Keyword><Keyword MajorTopicYN="N">Cell respiration</Keyword><Keyword MajorTopicYN="N">Intracellular oxygen</Keyword><Keyword MajorTopicYN="N">Oxygen consumption rate</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>7</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>8</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>9</Month><Day>4</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25233002</ArticleId><ArticleId IdType="pii">S0003-2697(14)00399-6</ArticleId><ArticleId IdType="doi">10.1016/j.ab.2014.09.001</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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