Avoidance of DNA methylation. A virus-encoded methylase inhibitor and evidence for counterselection of methylase recognition sites in viral genomes.
Identifieur interne : 004B06 ( Main/Exploration ); précédent : 004B05; suivant : 004B07Avoidance of DNA methylation. A virus-encoded methylase inhibitor and evidence for counterselection of methylase recognition sites in viral genomes.
Auteurs : D H Krüger [Allemagne] ; C. Schroeder ; M. Santibanez-Koref ; M. ReuterSource :
- Cell biophysics [ 0163-4992 ]
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : DNA Modification Methylases.
- chemical , genetics : Hydrolases.
- chemical , metabolism : DNA Modification Methylases, DNA, Viral, Hydrolases.
- enzymology : T-Phages.
- genetics : T-Phages.
- metabolism : T-Phages.
- Base Sequence, Genes, Viral, Methylation.
Abstract
The ocr+ gene of bacterial virus T7 codes for the first protein recognized to inhibit a specific group of DNA methylases. The recognition sequences of several other DNA methylases, not susceptible to Ocr inhibition, are significantly suppressed in the virus genome. The bacterial virus T3 encodes an Ado-Met hydrolase, destroying the methyl donor and causing T3 DNA to be totally unmethylated. These observations could stimulate analogous investigations into the regulation of DNA methylation patterns of eukaryotic viruses and cells. For instance, an underrepresentation of methylation sites (5'-CG) is also true for animal DNA viruses. Moreover, we were able to disclose some novel properties of DNA restriction-modification enzymes concerning the protection of DNA recognition sequences in which only one strand can be methylated (e.g., type III enzyme EcoP15) and the primary resistance of (unmethylated) DNA recognition sites towards type II restriction endonuclease EcoRII.
PubMed: 2476230
Affiliations:
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Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Avoidance of DNA methylation. A virus-encoded methylase inhibitor and evidence for counterselection of methylase recognition sites in viral genomes.</title><author><name sortKey="Kruger, D H" sort="Kruger, D H" uniqKey="Kruger D" first="D H" last="Krüger">D H Krüger</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Medical Virology, Humboldt University School of Medicine, Charité, Berlin, German Democratic Republic.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Virology, Humboldt University School of Medicine, Charité, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Schroeder, C" sort="Schroeder, C" uniqKey="Schroeder C" first="C" last="Schroeder">C. Schroeder</name></author><author><name sortKey="Santibanez Koref, M" sort="Santibanez Koref, M" uniqKey="Santibanez Koref M" first="M" last="Santibanez-Koref">M. Santibanez-Koref</name></author><author><name sortKey="Reuter, M" sort="Reuter, M" uniqKey="Reuter M" first="M" last="Reuter">M. Reuter</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>1989 Aug-Oct</MedlineDate></PubDate></date><idno type="RBID">pubmed:2476230</idno><idno type="pmid">2476230</idno><idno type="wicri:Area/PubMed/Corpus">000088</idno><idno type="wicri:Area/PubMed/Curation">000088</idno><idno type="wicri:Area/PubMed/Checkpoint">000088</idno><idno type="wicri:Area/Ncbi/Merge">000188</idno><idno type="wicri:Area/Ncbi/Curation">000188</idno><idno type="wicri:Area/Ncbi/Checkpoint">000188</idno><idno type="wicri:doubleKey">0163-4992::Kruger D:avoidance:of:dna</idno><idno type="wicri:Area/Main/Merge">004D09</idno><idno type="wicri:Area/Main/Curation">004B06</idno><idno type="wicri:Area/Main/Exploration">004B06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Avoidance of DNA methylation. A virus-encoded methylase inhibitor and evidence for counterselection of methylase recognition sites in viral genomes.</title><author><name sortKey="Kruger, D H" sort="Kruger, D H" uniqKey="Kruger D" first="D H" last="Krüger">D H Krüger</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Medical Virology, Humboldt University School of Medicine, Charité, Berlin, German Democratic Republic.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Virology, Humboldt University School of Medicine, Charité, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Schroeder, C" sort="Schroeder, C" uniqKey="Schroeder C" first="C" last="Schroeder">C. Schroeder</name></author><author><name sortKey="Santibanez Koref, M" sort="Santibanez Koref, M" uniqKey="Santibanez Koref M" first="M" last="Santibanez-Koref">M. Santibanez-Koref</name></author><author><name sortKey="Reuter, M" sort="Reuter, M" uniqKey="Reuter M" first="M" last="Reuter">M. Reuter</name></author></analytic><series><title level="j">Cell biophysics</title><idno type="ISSN">0163-4992</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>DNA Modification Methylases (antagonists & inhibitors)</term><term>DNA Modification Methylases (metabolism)</term><term>DNA, Viral (metabolism)</term><term>Genes, Viral</term><term>Hydrolases (genetics)</term><term>Hydrolases (metabolism)</term><term>Methylation</term><term>T-Phages (enzymology)</term><term>T-Phages (genetics)</term><term>T-Phages (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>DNA Modification Methylases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hydrolases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA Modification Methylases</term><term>DNA, Viral</term><term>Hydrolases</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>T-Phages</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>T-Phages</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>T-Phages</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Genes, Viral</term><term>Methylation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ocr+ gene of bacterial virus T7 codes for the first protein recognized to inhibit a specific group of DNA methylases. The recognition sequences of several other DNA methylases, not susceptible to Ocr inhibition, are significantly suppressed in the virus genome. The bacterial virus T3 encodes an Ado-Met hydrolase, destroying the methyl donor and causing T3 DNA to be totally unmethylated. These observations could stimulate analogous investigations into the regulation of DNA methylation patterns of eukaryotic viruses and cells. For instance, an underrepresentation of methylation sites (5'-CG) is also true for animal DNA viruses. Moreover, we were able to disclose some novel properties of DNA restriction-modification enzymes concerning the protection of DNA recognition sequences in which only one strand can be methylated (e.g., type III enzyme EcoP15) and the primary resistance of (unmethylated) DNA recognition sites towards type II restriction endonuclease EcoRII.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Berlin</li></region><settlement><li>Berlin</li></settlement></list><tree><noCountry><name sortKey="Reuter, M" sort="Reuter, M" uniqKey="Reuter M" first="M" last="Reuter">M. Reuter</name><name sortKey="Santibanez Koref, M" sort="Santibanez Koref, M" uniqKey="Santibanez Koref M" first="M" last="Santibanez-Koref">M. Santibanez-Koref</name><name sortKey="Schroeder, C" sort="Schroeder, C" uniqKey="Schroeder C" first="C" last="Schroeder">C. Schroeder</name></noCountry><country name="Allemagne"><region name="Berlin"><name sortKey="Kruger, D H" sort="Kruger, D H" uniqKey="Kruger D" first="D H" last="Krüger">D H Krüger</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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