Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region
Identifieur interne : 003682 ( Istex/Corpus ); précédent : 003681; suivant : 003683Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region
Auteurs : Gregory L. Blatch ; Renate R. Scholle ; David R. WoodsSource :
- Gene [ 0378-1119 ] ; 1990.
Abstract
The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr protein from Streptococcus mutants, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm.
Url:
DOI: 10.1016/0378-1119(90)90408-J
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region</title><author><name sortKey="Blatch, Gregory L" sort="Blatch, Gregory L" uniqKey="Blatch G" first="Gregory L." last="Blatch">Gregory L. Blatch</name><affiliation><mods:affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</mods:affiliation></affiliation></author><author><name sortKey="Scholle, Renate R" sort="Scholle, Renate R" uniqKey="Scholle R" first="Renate R." last="Scholle">Renate R. Scholle</name><affiliation><mods:affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</mods:affiliation></affiliation></author><author><name sortKey="Woods, David R" sort="Woods, David R" uniqKey="Woods D" first="David R." last="Woods">David R. Woods</name><affiliation><mods:affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:43C3427E988CA1CBF881954A89E28C53F7D120D6</idno><date when="1990" year="1990">1990</date><idno type="doi">10.1016/0378-1119(90)90408-J</idno><idno type="url">https://api.istex.fr/document/43C3427E988CA1CBF881954A89E28C53F7D120D6/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">003682</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region</title><author><name sortKey="Blatch, Gregory L" sort="Blatch, Gregory L" uniqKey="Blatch G" first="Gregory L." last="Blatch">Gregory L. Blatch</name><affiliation><mods:affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</mods:affiliation></affiliation></author><author><name sortKey="Scholle, Renate R" sort="Scholle, Renate R" uniqKey="Scholle R" first="Renate R." last="Scholle">Renate R. Scholle</name><affiliation><mods:affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</mods:affiliation></affiliation></author><author><name sortKey="Woods, David R" sort="Woods, David R" uniqKey="Woods D" first="David R." last="Woods">David R. Woods</name><affiliation><mods:affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="ISSN">0378-1119</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990">1990</date><biblScope unit="volume">95</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="17">17</biblScope><biblScope unit="page" to="23">23</biblScope></imprint><idno type="ISSN">0378-1119</idno></series><idno type="istex">43C3427E988CA1CBF881954A89E28C53F7D120D6</idno><idno type="DOI">10.1016/0378-1119(90)90408-J</idno><idno type="PII">0378-1119(90)90408-J</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1119</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr protein from Streptococcus mutants, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Gregory L. Blatch</name><affiliations><json:string>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</json:string></affiliations></json:item><json:item><name>Renate R. Scholle</name><affiliations><json:string>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</json:string></affiliations></json:item><json:item><name>David R. Woods</name><affiliations><json:string>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Bacillus subtilis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Streptococcus mutants</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>sucrase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>fructokinase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>transposon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>recombinant DNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>A600 : absorbance at 600 nm</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>aa : amino acid(s)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Ap : ampicillin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>B. : Bacillus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>bp : base pair(s)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Δ : deletion</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>EI, EII and EIII : enzymes I, II and III, respectively, of the PTS</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>EIIScr : enzyme IISucrose</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HPr : heat-stable protein</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>kb : kilobase(s) or 1000 bp</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LB : Luria-Bertani (medium)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>nt : nucleotide(s)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ORF : open reading frame</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>p : plasmid</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PEP : phosphoenolpyruvate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PTS : phosphotransferase system</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>S. : Streptococcus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>scrA : V. alginolyticus sucrose uptake-encoding gene</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>scrB : V. alginolyticus sucrase-encoding gene</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>scrK : V. alginolyticus fructokinase-encoding gene</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Tn : transposon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>V. : Vibrio</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>[] : denotes plasmid-carrier state</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>:: : novel joint (fusion)</value></json:item></subject><language><json:string>eng</json:string></language><abstract>The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr protein from Streptococcus mutants, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm.</abstract><qualityIndicators><score>5.459</score><pdfVersion>1.2</pdfVersion><pdfPageSize>591 x 814 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>30</keywordCount><abstractCharCount>823</abstractCharCount><pdfWordCount>4139</pdfWordCount><pdfCharCount>24717</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>110</abstractWordCount></qualityIndicators><title>Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region</title><pii><json:string>0378-1119(90)90408-J</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>84</volume><pages><last>8777</last><first>8773</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>Proc. Natl. Acad. Sci. USA</title></serie><host><volume>95</volume><pii><json:string>S0378-1119(00)X0513-6</json:string></pii><pages><last>23</last><first>17</first></pages><issn><json:string>0378-1119</json:string></issn><issue>1</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Gene</title><publicationDate>1990</publicationDate></host><categories><wos><json:string>BIOCHEMISTRY & MOLECULAR BIOLOGY</json:string><json:string>GENETICS & HEREDITY</json:string></wos></categories><publicationDate>1990</publicationDate><copyrightDate>1990</copyrightDate><doi><json:string>10.1016/0378-1119(90)90408-J</json:string></doi><id>43C3427E988CA1CBF881954A89E28C53F7D120D6</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/43C3427E988CA1CBF881954A89E28C53F7D120D6/fulltext/pdf</uri></json:item><json:item><original>true</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/43C3427E988CA1CBF881954A89E28C53F7D120D6/fulltext/txt</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/43C3427E988CA1CBF881954A89E28C53F7D120D6/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/43C3427E988CA1CBF881954A89E28C53F7D120D6/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1990</date></publicationStmt><notesStmt><note>Received by J. Davison</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region</title><author><persName><forename type="first">Gregory L.</forename><surname>Blatch</surname></persName><affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</affiliation></author><author><persName><forename type="first">Renate R.</forename><surname>Scholle</surname></persName><affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</affiliation></author><author><persName><forename type="first">David R.</forename><surname>Woods</surname></persName><note type="correspondence"><p>Correspondence to: Dr. D.R. Woods, Dept. of Microbiology, University of Cape Town, Rondebosch 7700, South Africa Tel. (021) 650-2173; Fax (021) 650-2138.</p></note><affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</affiliation></author></analytic><monogr><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="pISSN">0378-1119</idno><idno type="PII">S0378-1119(00)X0513-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990"></date><biblScope unit="volume">95</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="17">17</biblScope><biblScope unit="page" to="23">23</biblScope></imprint></monogr><idno type="istex">43C3427E988CA1CBF881954A89E28C53F7D120D6</idno><idno type="DOI">10.1016/0378-1119(90)90408-J</idno><idno type="PII">0378-1119(90)90408-J</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1990</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr protein from Streptococcus mutants, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Bacillus subtilis</term></item><item><term>Streptococcus mutants</term></item><item><term>sucrase</term></item><item><term>fructokinase</term></item><item><term>transposon</term></item><item><term>recombinant DNA</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>A600</term><term>absorbance at 600 nm</term></item><item><term>aa</term><term>amino acid(s)</term></item><item><term>Ap</term><term>ampicillin</term></item><item><term>B.</term><term>Bacillus</term></item><item><term>bp</term><term>base pair(s)</term></item><item><term>Δ</term><term>deletion</term></item><item><term>EI, EII and EIII</term><term>enzymes I, II and III, respectively, of the PTS</term></item><item><term>EIIScr</term><term>enzyme IISucrose</term></item><item><term>HPr</term><term>heat-stable protein</term></item><item><term>kb</term><term>kilobase(s) or 1000 bp</term></item><item><term>LB</term><term>Luria-Bertani (medium)</term></item><item><term>nt</term><term>nucleotide(s)</term></item><item><term>ORF</term><term>open reading frame</term></item><item><term>p</term><term>plasmid</term></item><item><term>PEP</term><term>phosphoenolpyruvate</term></item><item><term>PTS</term><term>phosphotransferase system</term></item><item><term>S.</term><term>Streptococcus</term></item><item><term>scrA</term><term>V. alginolyticus sucrose uptake-encoding gene</term></item><item><term>scrB</term><term>V. alginolyticus sucrase-encoding gene</term></item><item><term>scrK</term><term>V. alginolyticus fructokinase-encoding gene</term></item><item><term>Tn</term><term>transposon</term></item><item><term>V.</term><term>Vibrio</term></item><item><term>[]</term><term>denotes plasmid-carrier state</term></item><item><term>::</term><term>novel joint (fusion)</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1990-06-30">Registration</change><change when="1990-03-14">Modified</change><change when="1990">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" </istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>GENE</jid><aid>90408</aid><ce:pii>0378-1119(90)90408-J</ce:pii><ce:doi>10.1016/0378-1119(90)90408-J</ce:doi><ce:copyright type="unknown" year="1990"></ce:copyright></item-info><head><ce:title>Nucleotide sequence and analysis of the <ce:italic>Vibrio alginolyticus</ce:italic> sucrose uptake-encoding region</ce:title><ce:author-group><ce:author><ce:given-name>Gregory L.</ce:given-name><ce:surname>Blatch</ce:surname></ce:author><ce:author><ce:given-name>Renate R.</ce:given-name><ce:surname>Scholle</ce:surname></ce:author><ce:author><ce:given-name>David R.</ce:given-name><ce:surname>Woods</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: Dr. D.R. Woods, Dept. of Microbiology, University of Cape Town, Rondebosch 7700, South Africa Tel. (021) 650-2173; Fax (021) 650-2138.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="14" month="3" year="1990"></ce:date-received><ce:date-revised day="14" month="3" year="1990"></ce:date-revised><ce:date-accepted day="30" month="6" year="1990"></ce:date-accepted><ce:miscellaneous>Received by J. Davison</ce:miscellaneous><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para id="SP0005">The nucleotide sequence of the <ce:italic>Vibrio alginolyticus</ce:italic> sucrose uptake-encoding region was determined, and contained two genes, <ce:italic>scrA</ce:italic> and <ce:italic>scrK</ce:italic>. The <ce:italic>scrA</ce:italic> gene encodes an enzyme II<ce:sup>Sucrose</ce:sup> (EII<ce:sup>Scr</ce:sup>) protein of the phosphoenolpyruvate dependent phosphotransferase system and the <ce:italic>scrK</ce:italic> gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the <ce:italic>V. alginolyticus</ce:italic> EII<ce:sup>Scr</ce:sup> protein was homologous with the EII<ce:sup>Scr</ce:sup> protein from <ce:italic>Streptococcus mutants, Salmonella typhimurium</ce:italic> (pUR400 system) and <ce:italic>Bacillus subtilis</ce:italic>. The deduced aa sequence for the <ce:italic>V. alginolyticus</ce:italic> fructokinase was homologous with the <ce:italic>Escherichia coli</ce:italic> enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon <ce:italic>phoA</ce:italic> mutagenesis experiments indicated that the EII<ce:sup>Scr</ce:sup> protein was a membrane-bound protein with a region that extended into the periplasm.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>Bacillus subtilis</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>Streptococcus mutants</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>sucrase</ce:text></ce:keyword><ce:keyword><ce:text>fructokinase</ce:text></ce:keyword><ce:keyword><ce:text>transposon</ce:text></ce:keyword><ce:keyword><ce:text>recombinant DNA</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text><ce:italic>A</ce:italic><ce:inf>600</ce:inf></ce:text><ce:keyword><ce:text>absorbance at 600 nm</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>aa</ce:text><ce:keyword><ce:text>amino acid(s)</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>Ap</ce:text><ce:keyword><ce:text>ampicillin</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text><ce:italic>B.</ce:italic></ce:text><ce:keyword><ce:text><ce:italic>Bacillus</ce:italic></ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>bp</ce:text><ce:keyword><ce:text>base pair(s)</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>Δ</ce:text><ce:keyword><ce:text>deletion</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>EI, EII and EIII</ce:text><ce:keyword><ce:text>enzymes I, II and III, respectively, of the PTS</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>EII<ce:sup>Scr</ce:sup></ce:text><ce:keyword><ce:text>enzyme II<ce:sup>Sucrose</ce:sup></ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>HPr</ce:text><ce:keyword><ce:text>heat-stable protein</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>kb</ce:text><ce:keyword><ce:text>kilobase(s) or 1000 bp</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>LB</ce:text><ce:keyword><ce:text>Luria-Bertani (medium)</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>nt</ce:text><ce:keyword><ce:text>nucleotide(s)</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>ORF</ce:text><ce:keyword><ce:text>open reading frame</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>p</ce:text><ce:keyword><ce:text>plasmid</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>PEP</ce:text><ce:keyword><ce:text>phosphoenolpyruvate</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>PTS</ce:text><ce:keyword><ce:text>phosphotransferase system</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text><ce:italic>S.</ce:italic></ce:text><ce:keyword><ce:text><ce:italic>Streptococcus</ce:italic></ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text><ce:italic>scrA</ce:italic></ce:text><ce:keyword><ce:text><ce:italic>V. alginolyticus</ce:italic> sucrose uptake-encoding gene</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text><ce:italic>scrB</ce:italic></ce:text><ce:keyword><ce:text><ce:italic>V. alginolyticus</ce:italic> sucrase-encoding gene</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text><ce:italic>scrK</ce:italic></ce:text><ce:keyword><ce:text><ce:italic>V. alginolyticus</ce:italic> fructokinase-encoding gene</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>Tn</ce:text><ce:keyword><ce:text>transposon</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text><ce:italic>V.</ce:italic></ce:text><ce:keyword><ce:text><ce:italic>Vibrio</ce:italic></ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>[]</ce:text><ce:keyword><ce:text>denotes plasmid-carrier state</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>::</ce:text><ce:keyword><ce:text>novel joint (fusion)</ce:text></ce:keyword></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Nucleotide sequence and analysis of the</title></titleInfo><name type="personal"><namePart type="given">Gregory L.</namePart><namePart type="family">Blatch</namePart><affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Renate R.</namePart><namePart type="family">Scholle</namePart><affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">David R.</namePart><namePart type="family">Woods</namePart><affiliation>Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa</affiliation><description>Correspondence to: Dr. D.R. Woods, Dept. of Microbiology, University of Cape Town, Rondebosch 7700, South Africa Tel. (021) 650-2173; Fax (021) 650-2138.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued><dateValid encoding="w3cdtf">1990-06-30</dateValid><dateModified encoding="w3cdtf">1990-03-14</dateModified><copyrightDate encoding="w3cdtf">1990</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr protein from Streptococcus mutants, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm.</abstract><note>Received by J. Davison</note><subject><genre>Keywords</genre><topic>Bacillus subtilis</topic><topic>Streptococcus mutants</topic><topic>sucrase</topic><topic>fructokinase</topic><topic>transposon</topic><topic>recombinant DNA</topic></subject><subject><genre>Abbreviations</genre><topic>A600 : absorbance at 600 nm</topic><topic>aa : amino acid(s)</topic><topic>Ap : ampicillin</topic><topic>B. : Bacillus</topic><topic>bp : base pair(s)</topic><topic>Δ : deletion</topic><topic>EI, EII and EIII : enzymes I, II and III, respectively, of the PTS</topic><topic>EIIScr : enzyme IISucrose</topic><topic>HPr : heat-stable protein</topic><topic>kb : kilobase(s) or 1000 bp</topic><topic>LB : Luria-Bertani (medium)</topic><topic>nt : nucleotide(s)</topic><topic>ORF : open reading frame</topic><topic>p : plasmid</topic><topic>PEP : phosphoenolpyruvate</topic><topic>PTS : phosphotransferase system</topic><topic>S. : Streptococcus</topic><topic>scrA : V. alginolyticus sucrose uptake-encoding gene</topic><topic>scrB : V. alginolyticus sucrase-encoding gene</topic><topic>scrK : V. alginolyticus fructokinase-encoding gene</topic><topic>Tn : transposon</topic><topic>V. : Vibrio</topic><topic>[] : denotes plasmid-carrier state</topic><topic>:: : novel joint (fusion)</topic></subject><relatedItem type="host"><titleInfo><title>Gene</title></titleInfo><titleInfo type="abbreviated"><title>GENE</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19901030</dateIssued></originInfo><identifier type="ISSN">0378-1119</identifier><identifier type="PII">S0378-1119(00)X0513-6</identifier><part><date>19901030</date><detail type="volume"><number>95</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>163</end></extent><extent unit="pages"><start>17</start><end>23</end></extent></part></relatedItem><identifier type="istex">43C3427E988CA1CBF881954A89E28C53F7D120D6</identifier><identifier type="DOI">10.1016/0378-1119(90)90408-J</identifier><identifier type="PII">0378-1119(90)90408-J</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/43C3427E988CA1CBF881954A89E28C53F7D120D6/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">BIOCHEMISTRY & MOLECULAR BIOLOGY</classCode><classCode scheme="WOS">GENETICS & HEREDITY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
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