Surface elasticity imaging of vascular tissues in a liquid environment by a scanning haptic microscope.
Identifieur interne : 000E75 ( PubMed/Checkpoint ); précédent : 000E74; suivant : 000E76Surface elasticity imaging of vascular tissues in a liquid environment by a scanning haptic microscope.
Auteurs : Tomonori Oie [Japon] ; Hisato Suzuki ; Yoshinobu Murayama ; Toru Fukuda ; Sadao Omata ; Keiichi Kanda ; Keiichi Takamizawa ; Yasuhide NakayamaSource :
- Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs [ 1619-0904 ] ; 2010.
English descriptors
- KwdEn :
- MESH :
- physiology : Arteries, Extracellular Matrix.
- Animals, Elasticity, Microscopy, Swine.
Abstract
The objective of this study was to make an elasticity distribution image of natural arteries in a liquid environment at high resolution at the micrometer level and at a wide area at the sub-square millimeter level by improving the scanning haptic microscope (SHM), developed previously for characterization of the stiffness of natural tissues. The circumferential sections (thickness, 1.0 mm) of small-caliber porcine arteries (approximately 3-mm diameter) were used as a sample. Measurement was performed by soaking a probe (diameter, 5 microm; spatial resolution, less than 2 microm) in saline solution at an appropriate depth. The vascular tissues were segregated by multi-layering a high elasticity region with mainly elastin (50.8 +/- 13.8 kPa) and a low one with mainly collagen and smooth muscle cells (17.0 +/- 9.0 kPa), as observed previously in high humidity conditions. The elasticity was measured repeatedly with little change for over 4 h in a liquid environment, which enabled observation with maintenance of high precision of a large area of at least 1,200 x 100 microm, whereas the elasticity was increased with time by the dehydration of samples with shrinkage in the air, in which an averaged elasticity in the overall area was approximately doubled within 2 h. This simple, inexpensive system allows observation of the distribution of the surface elasticity at the extracellular matrix level of vascular tissues in a liquid environment close to the natural one.
DOI: 10.1007/s10047-010-0503-2
PubMed: 20473627
Affiliations:
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Yoshinobu" uniqKey="Murayama Y" first="Yoshinobu" last="Murayama">Yoshinobu Murayama</name></author><author><name sortKey="Fukuda, Toru" sort="Fukuda, Toru" uniqKey="Fukuda T" first="Toru" last="Fukuda">Toru Fukuda</name></author><author><name sortKey="Omata, Sadao" sort="Omata, Sadao" uniqKey="Omata S" first="Sadao" last="Omata">Sadao Omata</name></author><author><name sortKey="Kanda, Keiichi" sort="Kanda, Keiichi" uniqKey="Kanda K" first="Keiichi" last="Kanda">Keiichi Kanda</name></author><author><name sortKey="Takamizawa, Keiichi" sort="Takamizawa, Keiichi" uniqKey="Takamizawa K" first="Keiichi" last="Takamizawa">Keiichi Takamizawa</name></author><author><name sortKey="Nakayama, Yasuhide" sort="Nakayama, Yasuhide" uniqKey="Nakayama Y" first="Yasuhide" last="Nakayama">Yasuhide Nakayama</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="doi">10.1007/s10047-010-0503-2</idno><idno 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565-8565</wicri:regionArea><wicri:noRegion>565-8565</wicri:noRegion></affiliation></author><author><name sortKey="Suzuki, Hisato" sort="Suzuki, Hisato" uniqKey="Suzuki H" first="Hisato" last="Suzuki">Hisato Suzuki</name></author><author><name sortKey="Murayama, Yoshinobu" sort="Murayama, Yoshinobu" uniqKey="Murayama Y" first="Yoshinobu" last="Murayama">Yoshinobu Murayama</name></author><author><name sortKey="Fukuda, Toru" sort="Fukuda, Toru" uniqKey="Fukuda T" first="Toru" last="Fukuda">Toru Fukuda</name></author><author><name sortKey="Omata, Sadao" sort="Omata, Sadao" uniqKey="Omata S" first="Sadao" last="Omata">Sadao Omata</name></author><author><name sortKey="Kanda, Keiichi" sort="Kanda, Keiichi" uniqKey="Kanda K" first="Keiichi" last="Kanda">Keiichi Kanda</name></author><author><name sortKey="Takamizawa, Keiichi" sort="Takamizawa, Keiichi" uniqKey="Takamizawa K" first="Keiichi" last="Takamizawa">Keiichi Takamizawa</name></author><author><name sortKey="Nakayama, Yasuhide" sort="Nakayama, Yasuhide" uniqKey="Nakayama Y" first="Yasuhide" last="Nakayama">Yasuhide Nakayama</name></author></analytic><series><title level="j">Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs</title><idno type="eISSN">1619-0904</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Arteries (physiology)</term><term>Elasticity</term><term>Extracellular Matrix (physiology)</term><term>Microscopy</term><term>Swine</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Arteries</term><term>Extracellular Matrix</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Elasticity</term><term>Microscopy</term><term>Swine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objective of this study was to make an elasticity distribution image of natural arteries in a liquid environment at high resolution at the micrometer level and at a wide area at the sub-square millimeter level by improving the scanning haptic microscope (SHM), developed previously for characterization of the stiffness of natural tissues. The circumferential sections (thickness, 1.0 mm) of small-caliber porcine arteries (approximately 3-mm diameter) were used as a sample. Measurement was performed by soaking a probe (diameter, 5 microm; spatial resolution, less than 2 microm) in saline solution at an appropriate depth. The vascular tissues were segregated by multi-layering a high elasticity region with mainly elastin (50.8 +/- 13.8 kPa) and a low one with mainly collagen and smooth muscle cells (17.0 +/- 9.0 kPa), as observed previously in high humidity conditions. The elasticity was measured repeatedly with little change for over 4 h in a liquid environment, which enabled observation with maintenance of high precision of a large area of at least 1,200 x 100 microm, whereas the elasticity was increased with time by the dehydration of samples with shrinkage in the air, in which an averaged elasticity in the overall area was approximately doubled within 2 h. This simple, inexpensive system allows observation of the distribution of the surface elasticity at the extracellular matrix level of vascular tissues in a liquid environment close to the natural one.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">20473627</PMID><DateCreated><Year>2010</Year><Month>06</Month><Day>24</Day></DateCreated><DateCompleted><Year>2010</Year><Month>09</Month><Day>27</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1619-0904</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>2</Issue><PubDate><Year>2010</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs</Title><ISOAbbreviation>J Artif Organs</ISOAbbreviation></Journal><ArticleTitle>Surface elasticity imaging of vascular tissues in a liquid environment by a scanning haptic microscope.</ArticleTitle><Pagination><MedlinePgn>121-5</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10047-010-0503-2</ELocationID><Abstract><AbstractText>The objective of this study was to make an elasticity distribution image of natural arteries in a liquid environment at high resolution at the micrometer level and at a wide area at the sub-square millimeter level by improving the scanning haptic microscope (SHM), developed previously for characterization of the stiffness of natural tissues. The circumferential sections (thickness, 1.0 mm) of small-caliber porcine arteries (approximately 3-mm diameter) were used as a sample. Measurement was performed by soaking a probe (diameter, 5 microm; spatial resolution, less than 2 microm) in saline solution at an appropriate depth. The vascular tissues were segregated by multi-layering a high elasticity region with mainly elastin (50.8 +/- 13.8 kPa) and a low one with mainly collagen and smooth muscle cells (17.0 +/- 9.0 kPa), as observed previously in high humidity conditions. The elasticity was measured repeatedly with little change for over 4 h in a liquid environment, which enabled observation with maintenance of high precision of a large area of at least 1,200 x 100 microm, whereas the elasticity was increased with time by the dehydration of samples with shrinkage in the air, in which an averaged elasticity in the overall area was approximately doubled within 2 h. This simple, inexpensive system allows observation of the distribution of the surface elasticity at the extracellular matrix level of vascular tissues in a liquid environment close to the natural one.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Oie</LastName><ForeName>Tomonori</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka, 565-8565, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suzuki</LastName><ForeName>Hisato</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Murayama</LastName><ForeName>Yoshinobu</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Fukuda</LastName><ForeName>Toru</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Omata</LastName><ForeName>Sadao</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Kanda</LastName><ForeName>Keiichi</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Takamizawa</LastName><ForeName>Keiichi</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Nakayama</LastName><ForeName>Yasuhide</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>05</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Artif Organs</MedlineTA><NlmUniqueID>9815648</NlmUniqueID><ISSNLinking>1434-7229</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000818">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D001158">Arteries</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000502">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D004548">Elasticity</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005109">Extracellular Matrix</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000502">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D008853">Microscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013552">Swine</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>10</Month><Day>5</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>4</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2010</Year><Month>5</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1007/s10047-010-0503-2</ArticleId><ArticleId IdType="pubmed">20473627</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Fukuda, Toru" sort="Fukuda, Toru" uniqKey="Fukuda T" first="Toru" last="Fukuda">Toru Fukuda</name><name sortKey="Kanda, Keiichi" sort="Kanda, Keiichi" uniqKey="Kanda K" first="Keiichi" last="Kanda">Keiichi Kanda</name><name sortKey="Murayama, Yoshinobu" sort="Murayama, Yoshinobu" uniqKey="Murayama Y" first="Yoshinobu" last="Murayama">Yoshinobu Murayama</name><name sortKey="Nakayama, Yasuhide" sort="Nakayama, Yasuhide" uniqKey="Nakayama Y" first="Yasuhide" last="Nakayama">Yasuhide Nakayama</name><name sortKey="Omata, Sadao" sort="Omata, Sadao" uniqKey="Omata S" first="Sadao" last="Omata">Sadao Omata</name><name sortKey="Suzuki, Hisato" sort="Suzuki, Hisato" uniqKey="Suzuki H" first="Hisato" last="Suzuki">Hisato Suzuki</name><name sortKey="Takamizawa, Keiichi" sort="Takamizawa, Keiichi" uniqKey="Takamizawa K" first="Keiichi" last="Takamizawa">Keiichi Takamizawa</name></noCountry><country name="Japon"><noRegion><name sortKey="Oie, Tomonori" sort="Oie, Tomonori" uniqKey="Oie T" first="Tomonori" last="Oie">Tomonori Oie</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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