Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model
Identifieur interne : 000539 ( Main/Merge ); précédent : 000538; suivant : 000540Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model
Auteurs : Rita Mencucci [Italie] ; Eleonora Favuzza [Italie] ; Carlotta Boccalini [Italie] ; Jean-Jacques Gicquel [France] ; Laura Raimondi [Italie]Source :
- BMC Ophthalmology [ 1471-2415 ] ; 2015.
Abstract
To evaluate lens epithelial cell (LEC) proliferation with two different designs (one-piece or three-piece) of hydrophobic acrylic IOLs with 360° square optic edge using an in vitro culture model of posterior capsule opacification (PCO).
This experimental study was conducted at the Department of NEUROFARBA, Section of Pharmacology, University of Florence, Italy. Human LECs were seeded and cultured in transwell cell culture inserts coated with a type-IV collagen membrane on which an IOL (one-piece Tecnis-1 or three-piece AR40E, Abbott Medical Optics Inc.) had been previously placed. As control, cells were plated on the insert membrane without an IOL. At day six (cells confluent in controls) IOLs were removed and cell counting, viability and cell density under and outside the IOLs were evaluated.
No statistically significant difference in the number of cells (p > 0.05) between inserts with the one-piece and three-piece IOLs was found. Cell density in the area under each IOL was significantly lower than in the area outside of it (p < 0.05), or in the control insert. (p < 0.05). Cell density under the single-piece IOL was not significantly different from that under the three-piece IOL (p > 0.05).
A 360° sharp-edge played a crucial role in avoiding LEC migration under the IOL and preventing the formation of PCO after cataract surgery. Long term clinical evaluation is necessary to estimate functional results.
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DOI: 10.1186/1471-2415-15-5
PubMed: 25599704
PubMed Central: 4324805
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model</title><author><name sortKey="Mencucci, Rita" sort="Mencucci, Rita" uniqKey="Mencucci R" first="Rita" last="Mencucci">Rita Mencucci</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author><author><name sortKey="Favuzza, Eleonora" sort="Favuzza, Eleonora" uniqKey="Favuzza E" first="Eleonora" last="Favuzza">Eleonora Favuzza</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author><author><name sortKey="Boccalini, Carlotta" sort="Boccalini, Carlotta" uniqKey="Boccalini C" first="Carlotta" last="Boccalini">Carlotta Boccalini</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author><author><name sortKey="Gicquel, Jean Jacques" sort="Gicquel, Jean Jacques" uniqKey="Gicquel J" first="Jean-Jacques" last="Gicquel">Jean-Jacques Gicquel</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Ophthalmology, Poitiers University Hospital, Poitiers, Cedex, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Department of Ophthalmology, Poitiers University Hospital, Poitiers, Cedex</wicri:regionArea><wicri:noRegion>Cedex</wicri:noRegion><wicri:noRegion>Cedex</wicri:noRegion></affiliation></author><author><name sortKey="Raimondi, Laura" sort="Raimondi, Laura" uniqKey="Raimondi L" first="Laura" last="Raimondi">Laura Raimondi</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of NEUROFARBA, section of Pharmacology, University of Florence, Florence, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of NEUROFARBA, section of Pharmacology, University of Florence, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25599704</idno><idno type="pmc">4324805</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4324805</idno><idno type="RBID">PMC:4324805</idno><idno type="doi">10.1186/1471-2415-15-5</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000757</idno><idno type="wicri:Area/Pmc/Curation">000757</idno><idno type="wicri:Area/Pmc/Checkpoint">000321</idno><idno type="wicri:Area/Ncbi/Merge">003632</idno><idno type="wicri:Area/Ncbi/Curation">003632</idno><idno type="wicri:Area/Ncbi/Checkpoint">003632</idno><idno type="wicri:Area/Main/Merge">000539</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model</title><author><name sortKey="Mencucci, Rita" sort="Mencucci, Rita" uniqKey="Mencucci R" first="Rita" last="Mencucci">Rita Mencucci</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author><author><name sortKey="Favuzza, Eleonora" sort="Favuzza, Eleonora" uniqKey="Favuzza E" first="Eleonora" last="Favuzza">Eleonora Favuzza</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author><author><name sortKey="Boccalini, Carlotta" sort="Boccalini, Carlotta" uniqKey="Boccalini C" first="Carlotta" last="Boccalini">Carlotta Boccalini</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author><author><name sortKey="Gicquel, Jean Jacques" sort="Gicquel, Jean Jacques" uniqKey="Gicquel J" first="Jean-Jacques" last="Gicquel">Jean-Jacques Gicquel</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Ophthalmology, Poitiers University Hospital, Poitiers, Cedex, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Department of Ophthalmology, Poitiers University Hospital, Poitiers, Cedex</wicri:regionArea><wicri:noRegion>Cedex</wicri:noRegion><wicri:noRegion>Cedex</wicri:noRegion></affiliation></author><author><name sortKey="Raimondi, Laura" sort="Raimondi, Laura" uniqKey="Raimondi L" first="Laura" last="Raimondi">Laura Raimondi</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of NEUROFARBA, section of Pharmacology, University of Florence, Florence, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of NEUROFARBA, section of Pharmacology, University of Florence, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC Ophthalmology</title><idno type="eISSN">1471-2415</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>To evaluate lens epithelial cell (LEC) proliferation with two different designs (one-piece or three-piece) of hydrophobic acrylic IOLs with 360° square optic edge using an in vitro culture model of posterior capsule opacification (PCO).</p></sec><sec><title>Methods</title><p>This experimental study was conducted at the Department of NEUROFARBA, Section of Pharmacology, University of Florence, Italy. Human LECs were seeded and cultured in transwell cell culture inserts coated with a type-IV collagen membrane on which an IOL (one-piece Tecnis-1 or three-piece AR40E, Abbott Medical Optics Inc.) had been previously placed. As control, cells were plated on the insert membrane without an IOL. At day six (cells confluent in controls) IOLs were removed and cell counting, viability and cell density under and outside the IOLs were evaluated.</p></sec><sec><title>Results</title><p>No statistically significant difference in the number of cells (p > 0.05) between inserts with the one-piece and three-piece IOLs was found. Cell density in the area under each IOL was significantly lower than in the area outside of it (p < 0.05), or in the control insert. (p < 0.05). Cell density under the single-piece IOL was not significantly different from that under the three-piece IOL (p > 0.05).</p></sec><sec><title>Conclusions</title><p>A 360° sharp-edge played a crucial role in avoiding LEC migration under the IOL and preventing the formation of PCO after cataract surgery. 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