Lens Epithelial Cell Proliferation in Human Posterior Capsule Opacification Specimens
Identifieur interne : 004238 ( Istex/Curation ); précédent : 004237; suivant : 004239Lens Epithelial Cell Proliferation in Human Posterior Capsule Opacification Specimens
Auteurs : Jean-Marie Rakic [Belgique] ; Albert Galand [Belgique] ; Gijs F. J. M Vrensen [Pays-Bas]Source :
- Experimental Eye Research [ 0014-4835 ] ; 2000.
English descriptors
Abstract
In previous in vitro studies on capsular bags it was shown that, after a sham extracapsular cataract extraction (ECCE) on human donor eyes, lens epithelial cells (LECs) show, in the short term, a dramatically elevated mitotic activity as compared to that in the intact lens. The long term in vivo proliferation of LECs in human lenses after ECCE and intraocular lens (IOL) implantation has not been studied until now. In the present study, the mitotic activity of LECs in human post-mortem eyes with posterior capsule opacification (PCO) was investigated. Human lenses with signs of PCO were dissected from donor eyes and incubated in MEM, supplemented with fetal calf serum, for 1 day (n=10) or 7 days (n=9). Six additional specimens were cultured for 7 days after removal of the IOL and lens fibres. After the incubation period, mitotic activity was estimated using the BrdU procedure and the Ki67 proliferating cell marker. The mean number of BrdU-positive nuclei in the intact PCO specimens was at a level of 7.5 (day 1) and 6.5 (day 7). Removal of the IOL and the lens fibres leads to a ten-fold increase in BrdU positive cells (mean=84.5). No correlation with donor age was found. The Ki67 observations corroborate the BrdU results. The results demonstrate that after an initial rise in proliferative activity, as shown in the capsular bag model, the mitotic activity of LECs returns to a rate comparable to that in intact cultured non-cataractous lenses. As in control lenses, removal of lens fibres significantly elevated the proliferative activity of the remaining LECs. Suppression by newly formed differentiated lens fibres in the in vivo capsular bag may be responsible for this return to control levels of mitotic activity of LECs in the PCO specimens.
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DOI: 10.1006/exer.2000.0904
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lens Epithelial Cell Proliferation in Human Posterior Capsule Opacification Specimens</title><author><name sortKey="Rakic, Jean Marie" sort="Rakic, Jean Marie" uniqKey="Rakic J" first="Jean-Marie" last="Rakic">Jean-Marie Rakic</name><affiliation wicri:level="1"><mods:affiliation>Department of Ophthalmology, University of Liège, Belgium</mods:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Ophthalmology, University of Liège</wicri:regionArea></affiliation></author><author><name sortKey="Galand, Albert" sort="Galand, Albert" uniqKey="Galand A" first="Albert" last="Galand">Albert Galand</name><affiliation wicri:level="1"><mods:affiliation>Department of Ophthalmology, University of Liège, Belgium</mods:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Ophthalmology, University of Liège</wicri:regionArea></affiliation></author><author><name sortKey="Vrensen, Gijs F J M" sort="Vrensen, Gijs F J M" uniqKey="Vrensen G" first="Gijs F. 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J. M" last="Vrensen">Gijs F. J. M Vrensen</name><affiliation wicri:level="1"><mods:affiliation>The Netherlands Ophthalmic Research Institute, Amsterdam, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>The Netherlands Ophthalmic Research Institute, Amsterdam</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Experimental Eye Research</title><title level="j" type="abbrev">YEXER</title><idno type="ISSN">0014-4835</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">71</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="489">489</biblScope><biblScope unit="page" to="494">494</biblScope></imprint><idno type="ISSN">0014-4835</idno></series><idno type="istex">D89BC1071FD13AC713F5E4BC5D94704370E4C3BB</idno><idno type="DOI">10.1006/exer.2000.0904</idno><idno type="PII">S0014-4835(00)90904-7</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0014-4835</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>cell proliferation</term><term>human lens</term><term>posterior capsule opacification (after-cataract)</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In previous in vitro studies on capsular bags it was shown that, after a sham extracapsular cataract extraction (ECCE) on human donor eyes, lens epithelial cells (LECs) show, in the short term, a dramatically elevated mitotic activity as compared to that in the intact lens. The long term in vivo proliferation of LECs in human lenses after ECCE and intraocular lens (IOL) implantation has not been studied until now. In the present study, the mitotic activity of LECs in human post-mortem eyes with posterior capsule opacification (PCO) was investigated. Human lenses with signs of PCO were dissected from donor eyes and incubated in MEM, supplemented with fetal calf serum, for 1 day (n=10) or 7 days (n=9). Six additional specimens were cultured for 7 days after removal of the IOL and lens fibres. After the incubation period, mitotic activity was estimated using the BrdU procedure and the Ki67 proliferating cell marker. The mean number of BrdU-positive nuclei in the intact PCO specimens was at a level of 7.5 (day 1) and 6.5 (day 7). Removal of the IOL and the lens fibres leads to a ten-fold increase in BrdU positive cells (mean=84.5). No correlation with donor age was found. The Ki67 observations corroborate the BrdU results. The results demonstrate that after an initial rise in proliferative activity, as shown in the capsular bag model, the mitotic activity of LECs returns to a rate comparable to that in intact cultured non-cataractous lenses. As in control lenses, removal of lens fibres significantly elevated the proliferative activity of the remaining LECs. Suppression by newly formed differentiated lens fibres in the in vivo capsular bag may be responsible for this return to control levels of mitotic activity of LECs in the PCO specimens.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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