Changes in macrophage function and morphology due to biomedical polyurethane surfaces undergoing biodegradation
Identifieur interne : 001E33 ( Istex/Curation ); précédent : 001E32; suivant : 001E34Changes in macrophage function and morphology due to biomedical polyurethane surfaces undergoing biodegradation
Auteurs : Loren A. Matheson [Canada] ; J. Paul Santerre [Canada] ; Rosalind S. Labow [Canada]Source :
- Journal of Cellular Physiology [ 0021-9541 ] ; 2004-04.
Abstract
Monocytes are recruited to the material surface of an implanted biomedical device recognizing it as a foreign body. Differentiation into macrophages subsequently occurs followed by fusion to form foreign body giant cells (FBGCs). Consequently, implants can become degraded, cause chronic inflammation or become isolated by fibrous encapsulation. In this study, a relationship between material surface chemistry and the FBGC response was demonstrated by seeding mature monocyte‐derived macrophages (MDMs) on polycarbonate‐based polyurethanes that differed in their chemical structures (synthesized with poly(1,6‐hexyl 1,2‐ethyl carbonate) diol, and either 14C‐hexane diisocyanate and butanediol (BD) (referred to as HDI) or 4,4′‐methylene bisphenyl diisocyanate and 14C‐BD (referred to as MDI)) and material degradation assessed. At 48 h of cell‐material interaction, the FBGC attached to HDI were more multinucleated (73%) compared to MDI or the polystyrene (PS) control (21 and 36%, respectively). There was a fivefold increase in the synthesis and secretion of a protein with an approximate molecular weight of 48 kDa and a pI of 6.1 (determined by two‐dimensional gel electrophoresis) only from cells seeded on HDI. Immunoprecipitation confirmed that MSE and CE were synthesized and secreted de novo. Immunoblotting also showed an increase in secreted monocyte‐specific esterase (MSE) and cholesterol esterase (CE) from cells seeded on HDI relative to PS and MDI. Significantly more radiolabel (14C) release and esterase activity were elicited by MDMs on HDI than MDI (P < 0.05). The material that was more degradable (HDI), elicited greater protein synthesis and esterase secretion as well as more multinucleated MDMs than MDI, suggesting that the material surface chemistry modulates the function of MDM at the site of an inflammatory response to an implanted device. J. Cell. Physiol. 199: 8–19, 2004© 2003 Wiley‐Liss, Inc.
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DOI: 10.1002/jcp.10412
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Changes in macrophage function and morphology due to biomedical polyurethane surfaces undergoing biodegradation</title><author><name sortKey="Matheson, Loren A" sort="Matheson, Loren A" uniqKey="Matheson L" first="Loren A." last="Matheson">Loren A. Matheson</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario</wicri:regionArea></affiliation></author><author><name sortKey="Santerre, J Paul" sort="Santerre, J Paul" uniqKey="Santerre J" first="J. Paul" last="Santerre">J. Paul Santerre</name><affiliation wicri:level="1"><mods:affiliation>Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Faculty of Dentistry, University of Toronto, Toronto, Ontario</wicri:regionArea></affiliation></author><author><name sortKey="Labow, Rosalind S" sort="Labow, Rosalind S" uniqKey="Labow R" first="Rosalind S." last="Labow">Rosalind S. Labow</name><affiliation wicri:level="1"><mods:affiliation>University of Ottawa Heart Institute, Ottawa, Ontario, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>University of Ottawa Heart Institute, Ottawa, Ontario</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4C858914B7D3A6774D0FB3D562879D2F978E86AD</idno><date when="2004" year="2004">2004</date><idno type="doi">10.1002/jcp.10412</idno><idno type="url">https://api.istex.fr/document/4C858914B7D3A6774D0FB3D562879D2F978E86AD/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001E33</idno><idno type="wicri:Area/Istex/Curation">001E33</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Changes in macrophage function and morphology due to biomedical polyurethane surfaces undergoing biodegradation</title><author><name sortKey="Matheson, Loren A" sort="Matheson, Loren A" uniqKey="Matheson L" first="Loren A." last="Matheson">Loren A. Matheson</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario</wicri:regionArea></affiliation></author><author><name sortKey="Santerre, J Paul" sort="Santerre, J Paul" uniqKey="Santerre J" first="J. Paul" last="Santerre">J. Paul Santerre</name><affiliation wicri:level="1"><mods:affiliation>Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Faculty of Dentistry, University of Toronto, Toronto, Ontario</wicri:regionArea></affiliation></author><author><name sortKey="Labow, Rosalind S" sort="Labow, Rosalind S" uniqKey="Labow R" first="Rosalind S." last="Labow">Rosalind S. Labow</name><affiliation wicri:level="1"><mods:affiliation>University of Ottawa Heart Institute, Ottawa, Ontario, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>University of Ottawa Heart Institute, Ottawa, Ontario</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Cellular Physiology</title><title level="j" type="abbrev">J. Cell. Physiol.</title><idno type="ISSN">0021-9541</idno><idno type="eISSN">1097-4652</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2004-04">2004-04</date><biblScope unit="volume">199</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="8">8</biblScope><biblScope unit="page" to="19">19</biblScope></imprint><idno type="ISSN">0021-9541</idno></series><idno type="istex">4C858914B7D3A6774D0FB3D562879D2F978E86AD</idno><idno type="DOI">10.1002/jcp.10412</idno><idno type="ArticleID">JCP10412</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9541</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Monocytes are recruited to the material surface of an implanted biomedical device recognizing it as a foreign body. Differentiation into macrophages subsequently occurs followed by fusion to form foreign body giant cells (FBGCs). Consequently, implants can become degraded, cause chronic inflammation or become isolated by fibrous encapsulation. In this study, a relationship between material surface chemistry and the FBGC response was demonstrated by seeding mature monocyte‐derived macrophages (MDMs) on polycarbonate‐based polyurethanes that differed in their chemical structures (synthesized with poly(1,6‐hexyl 1,2‐ethyl carbonate) diol, and either 14C‐hexane diisocyanate and butanediol (BD) (referred to as HDI) or 4,4′‐methylene bisphenyl diisocyanate and 14C‐BD (referred to as MDI)) and material degradation assessed. At 48 h of cell‐material interaction, the FBGC attached to HDI were more multinucleated (73%) compared to MDI or the polystyrene (PS) control (21 and 36%, respectively). There was a fivefold increase in the synthesis and secretion of a protein with an approximate molecular weight of 48 kDa and a pI of 6.1 (determined by two‐dimensional gel electrophoresis) only from cells seeded on HDI. Immunoprecipitation confirmed that MSE and CE were synthesized and secreted de novo. Immunoblotting also showed an increase in secreted monocyte‐specific esterase (MSE) and cholesterol esterase (CE) from cells seeded on HDI relative to PS and MDI. Significantly more radiolabel (14C) release and esterase activity were elicited by MDMs on HDI than MDI (P < 0.05). The material that was more degradable (HDI), elicited greater protein synthesis and esterase secretion as well as more multinucleated MDMs than MDI, suggesting that the material surface chemistry modulates the function of MDM at the site of an inflammatory response to an implanted device. J. Cell. Physiol. 199: 8–19, 2004© 2003 Wiley‐Liss, Inc.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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