Modification of the surface properties of a lens material to influence posterior capsular opacification
Identifieur interne : 003C79 ( Istex/Corpus ); précédent : 003C78; suivant : 003C80Modification of the surface properties of a lens material to influence posterior capsular opacification
Auteurs : Conrad Yuen ; Rachel Williams ; Mark Batterbury ; Ian GriersonSource :
- Clinical & Experimental Ophthalmology [ 1442-6404 ] ; 2006-08.
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- KwdEn :
Abstract
Purpose: To study the effect of surface properties of materials on cellular behaviour and the formation of posterior capsular opacification (PCO). Methods: Polymethylmethacrylate, silicone and a hydrophobic acrylic were plasma treated and used in tissue culture. The changes in surface properties were quantified by dynamic contact angle measurements. Bovine lens epithelial cells (BLECs) were seeded onto these materials and cultured for 1 month. Serial photographs were taken. The cells were then fixed and stained to facilitate counting. Results: Plasma treatment significantly increased the hydrophilicity of surfaces. BLECs grew on all surfaces but significantly more cells adhered to the treated than the untreated surfaces. On the untreated surfaces the BLECs had a fibroblastic morphology whereas on the treated surfaces the cells maintained their epithelial morphology. Conclusions: Posterior capsular opacification is a form of wound healing and the behaviour of lens epithelial cells is central to its progression. Emphasis has been on the elimination of residual lens epithelial cells to combat PCO. This study demonstrated that the phenotype of BLECs was influenced by the surface properties of the intraocular lens materials. Gas plasma treatment of the materials increased their hydrophilicity and allowed the adhered BLECs to maintain their normal epithelial morphology. We believe that controlled growth of lens epithelial cells may reduce the incidence of PCO.
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DOI: 10.1111/j.1442-9071.2006.01278.x
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Methods: Polymethylmethacrylate, silicone and a hydrophobic acrylic were plasma treated and used in tissue culture. The changes in surface properties were quantified by dynamic contact angle measurements. Bovine lens epithelial cells (BLECs) were seeded onto these materials and cultured for 1 month. Serial photographs were taken. The cells were then fixed and stained to facilitate counting. Results: Plasma treatment significantly increased the hydrophilicity of surfaces. BLECs grew on all surfaces but significantly more cells adhered to the treated than the untreated surfaces. On the untreated surfaces the BLECs had a fibroblastic morphology whereas on the treated surfaces the cells maintained their epithelial morphology. Conclusions: Posterior capsular opacification is a form of wound healing and the behaviour of lens epithelial cells is central to its progression. Emphasis has been on the elimination of residual lens epithelial cells to combat PCO. This study demonstrated that the phenotype of BLECs was influenced by the surface properties of the intraocular lens materials. Gas plasma treatment of the materials increased their hydrophilicity and allowed the adhered BLECs to maintain their normal epithelial morphology. We believe that controlled growth of lens epithelial cells may reduce the incidence of PCO.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Conrad Yuen MRCOphth</name><affiliations><json:string>Ophthalmology, University Clinical Departments, and</json:string></affiliations></json:item><json:item><name>Rachel Williams PhD</name><affiliations><json:string>Clinical Engineering, School of Clinical Sciences, University of Liverpool, Liverpool, UK</json:string></affiliations></json:item><json:item><name>Mark Batterbury FRCOphth</name><affiliations><json:string>Ophthalmology, University Clinical Departments, and</json:string></affiliations></json:item><json:item><name>Ian Grierson PhD</name><affiliations><json:string>Ophthalmology, University Clinical Departments, and</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>posterior capsular opacification</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cellular morphology</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>gas plasma treatment</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>surface properties</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>intraocular lenses</value></json:item></subject><articleId><json:string>CEO1278</json:string></articleId><language><json:string>eng</json:string></language><abstract>Purpose: To study the effect of surface properties of materials on cellular behaviour and the formation of posterior capsular opacification (PCO). Methods: Polymethylmethacrylate, silicone and a hydrophobic acrylic were plasma treated and used in tissue culture. The changes in surface properties were quantified by dynamic contact angle measurements. Bovine lens epithelial cells (BLECs) were seeded onto these materials and cultured for 1 month. Serial photographs were taken. The cells were then fixed and stained to facilitate counting. Results: Plasma treatment significantly increased the hydrophilicity of surfaces. BLECs grew on all surfaces but significantly more cells adhered to the treated than the untreated surfaces. On the untreated surfaces the BLECs had a fibroblastic morphology whereas on the treated surfaces the cells maintained their epithelial morphology. Conclusions: Posterior capsular opacification is a form of wound healing and the behaviour of lens epithelial cells is central to its progression. Emphasis has been on the elimination of residual lens epithelial cells to combat PCO. This study demonstrated that the phenotype of BLECs was influenced by the surface properties of the intraocular lens materials. Gas plasma treatment of the materials increased their hydrophilicity and allowed the adhered BLECs to maintain their normal epithelial morphology. 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Email:</p></note><affiliation>Ophthalmology, University Clinical Departments, and</affiliation></author><author><persName><forename type="first">Rachel</forename><surname>Williams</surname></persName><roleName type="degree">PhD</roleName><affiliation>Clinical Engineering, School of Clinical Sciences, University of Liverpool, Liverpool, UK</affiliation></author><author><persName><forename type="first">Mark</forename><surname>Batterbury</surname></persName><roleName type="degree">FRCOphth</roleName><affiliation>Ophthalmology, University Clinical Departments, and</affiliation></author><author><persName><forename type="first">Ian</forename><surname>Grierson</surname></persName><roleName type="degree">PhD</roleName><affiliation>Ophthalmology, University Clinical Departments, and</affiliation></author></analytic><monogr><title level="j">Clinical & Experimental Ophthalmology</title><idno type="pISSN">1442-6404</idno><idno type="eISSN">1442-9071</idno><idno type="DOI">10.1111/(ISSN)1442-9071</idno><imprint><publisher>Blackwell Publishing Asia</publisher><pubPlace>Melbourne, Australia</pubPlace><date type="published" when="2006-08"></date><biblScope unit="volume">34</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="568">568</biblScope><biblScope unit="page" to="574">574</biblScope></imprint></monogr><idno type="istex">C1E093AA3E847852CFDF1DC07AB6981716477330</idno><idno type="DOI">10.1111/j.1442-9071.2006.01278.x</idno><idno type="ArticleID">CEO1278</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2006</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Purpose: To study the effect of surface properties of materials on cellular behaviour and the formation of posterior capsular opacification (PCO). Methods: Polymethylmethacrylate, silicone and a hydrophobic acrylic were plasma treated and used in tissue culture. The changes in surface properties were quantified by dynamic contact angle measurements. Bovine lens epithelial cells (BLECs) were seeded onto these materials and cultured for 1 month. Serial photographs were taken. The cells were then fixed and stained to facilitate counting. Results: Plasma treatment significantly increased the hydrophilicity of surfaces. BLECs grew on all surfaces but significantly more cells adhered to the treated than the untreated surfaces. On the untreated surfaces the BLECs had a fibroblastic morphology whereas on the treated surfaces the cells maintained their epithelial morphology. Conclusions: Posterior capsular opacification is a form of wound healing and the behaviour of lens epithelial cells is central to its progression. Emphasis has been on the elimination of residual lens epithelial cells to combat PCO. This study demonstrated that the phenotype of BLECs was influenced by the surface properties of the intraocular lens materials. Gas plasma treatment of the materials increased their hydrophilicity and allowed the adhered BLECs to maintain their normal epithelial morphology. We believe that controlled growth of lens epithelial cells may reduce the incidence of PCO.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>posterior capsular opacification</term></item><item><term>cellular morphology</term></item><item><term>gas plasma treatment</term></item><item><term>surface properties</term></item><item><term>intraocular lenses</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2006-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/C1E093AA3E847852CFDF1DC07AB6981716477330/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Asia</publisherName><publisherLoc>Melbourne, Australia</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1442-9071</doi><issn type="print">1442-6404</issn><issn type="electronic">1442-9071</issn><idGroup><id type="product" value="CEO"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="CLINICAL EXPERIMENTAL OPHTHALMOLOGY">Clinical & Experimental Ophthalmology</title></titleGroup></publicationMeta><publicationMeta level="part" position="08006"><doi origin="wiley">10.1111/ceo.2006.34.issue-6</doi><numberingGroup><numbering type="journalVolume" number="34">34</numbering><numbering type="journalIssue" number="6">6</numbering></numberingGroup><coverDate startDate="2006-08">August 2006</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="12" status="forIssue"><doi origin="wiley">10.1111/j.1442-9071.2006.01278.x</doi><idGroup><id type="unit" value="CEO1278"></id></idGroup><countGroup><count type="pageTotal" number="7"></count></countGroup><titleGroup><title type="tocHeading1">Original Article</title><title type="tocHeading2">Laboratory Science</title></titleGroup><eventGroup><event type="firstOnline" date="2006-08-17"></event><event type="publishedOnlineFinalForm" date="2006-08-17"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.4 mode:FullText source:FullText result:FullText" date="2010-03-30"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-09"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="568">568</numbering><numbering type="pageLast" number="574">574</numbering></numberingGroup><correspondenceTo>Mr Conrad HW Yuen, Unit of Ophthalmology, Department of Medicine, University Clinical Departments, Daulby Street, Liverpool L69 3GA, UK. Email: <email>c.yuen@liverpool.ac.uk</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:CEO.CEO1278.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 1 August 2005; accepted 4 April 2006.</unparsedEditorialHistory><countGroup><count type="figureTotal" number="7"></count><count type="tableTotal" number="2"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="43"></count><count type="wordTotal" number="4154"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Modification of the surface properties of a lens material to influence posterior capsular opacification</title><title type="shortAuthors">Yuen <i>et al.</i></title><title type="short">Effect of surface properties of lens materials</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" corresponding="yes"><personName><givenNames>Conrad</givenNames><familyName>Yuen</familyName><degrees>MRCOphth</degrees></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>Rachel</givenNames><familyName>Williams</familyName><degrees>PhD</degrees></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Mark</givenNames><familyName>Batterbury</familyName><degrees>FRCOphth</degrees></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>Ian</givenNames><familyName>Grierson</familyName><degrees>PhD</degrees></personName></creator></creators><affiliationGroup><affiliation xml:id="a1"><unparsedAffiliation>Ophthalmology, University Clinical Departments, and</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="GB"><unparsedAffiliation>Clinical Engineering, School of Clinical Sciences, University of Liverpool, Liverpool, UK</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">posterior capsular opacification</keyword><keyword xml:id="k2">cellular morphology</keyword><keyword xml:id="k3">gas plasma treatment</keyword><keyword xml:id="k4">surface properties</keyword><keyword xml:id="k5">intraocular lenses</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">A<sc>bstract</sc></title><p><b>Purpose: </b> To study the effect of surface properties of materials on cellular behaviour and the formation of posterior capsular opacification (PCO).</p><p><b>Methods: </b> Polymethylmethacrylate, silicone and a hydrophobic acrylic were plasma treated and used in tissue culture. The changes in surface properties were quantified by dynamic contact angle measurements. Bovine lens epithelial cells (BLECs) were seeded onto these materials and cultured for 1 month. Serial photographs were taken. The cells were then fixed and stained to facilitate counting.</p><p><b>Results: </b> Plasma treatment significantly increased the hydrophilicity of surfaces. BLECs grew on all surfaces but significantly more cells adhered to the treated than the untreated surfaces. On the untreated surfaces the BLECs had a fibroblastic morphology whereas on the treated surfaces the cells maintained their epithelial morphology.</p><p><b>Conclusions: </b> Posterior capsular opacification is a form of wound healing and the behaviour of lens epithelial cells is central to its progression. Emphasis has been on the elimination of residual lens epithelial cells to combat PCO. This study demonstrated that the phenotype of BLECs was influenced by the surface properties of the intraocular lens materials. Gas plasma treatment of the materials increased their hydrophilicity and allowed the adhered BLECs to maintain their normal epithelial morphology. We believe that controlled growth of lens epithelial cells may reduce the incidence of PCO.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Modification of the surface properties of a lens material to influence posterior capsular opacification</title></titleInfo><titleInfo type="abbreviated"><title>Effect of surface properties of lens materials</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Modification of the surface properties of a lens material to influence posterior capsular opacification</title></titleInfo><name type="personal"><namePart type="given">Conrad</namePart><namePart type="family">Yuen</namePart><namePart type="termsOfAddress">MRCOphth</namePart><affiliation>Ophthalmology, University Clinical Departments, and</affiliation><description>Correspondence: Mr Conrad HW Yuen, Unit of Ophthalmology, Department of Medicine, University Clinical Departments, Daulby Street, Liverpool L69 3GA, UK. Email: </description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rachel</namePart><namePart type="family">Williams</namePart><namePart type="termsOfAddress">PhD</namePart><affiliation>Clinical Engineering, School of Clinical Sciences, University of Liverpool, Liverpool, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mark</namePart><namePart type="family">Batterbury</namePart><namePart type="termsOfAddress">FRCOphth</namePart><affiliation>Ophthalmology, University Clinical Departments, and</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ian</namePart><namePart type="family">Grierson</namePart><namePart type="termsOfAddress">PhD</namePart><affiliation>Ophthalmology, University Clinical Departments, and</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Asia</publisher><place><placeTerm type="text">Melbourne, Australia</placeTerm></place><dateIssued encoding="w3cdtf">2006-08</dateIssued><edition>Received 1 August 2005; accepted 4 April 2006.</edition><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">7</extent><extent unit="tables">2</extent><extent unit="references">43</extent><extent unit="words">4154</extent></physicalDescription><abstract lang="en">Purpose: To study the effect of surface properties of materials on cellular behaviour and the formation of posterior capsular opacification (PCO). Methods: Polymethylmethacrylate, silicone and a hydrophobic acrylic were plasma treated and used in tissue culture. The changes in surface properties were quantified by dynamic contact angle measurements. Bovine lens epithelial cells (BLECs) were seeded onto these materials and cultured for 1 month. Serial photographs were taken. The cells were then fixed and stained to facilitate counting. Results: Plasma treatment significantly increased the hydrophilicity of surfaces. BLECs grew on all surfaces but significantly more cells adhered to the treated than the untreated surfaces. On the untreated surfaces the BLECs had a fibroblastic morphology whereas on the treated surfaces the cells maintained their epithelial morphology. Conclusions: Posterior capsular opacification is a form of wound healing and the behaviour of lens epithelial cells is central to its progression. Emphasis has been on the elimination of residual lens epithelial cells to combat PCO. This study demonstrated that the phenotype of BLECs was influenced by the surface properties of the intraocular lens materials. Gas plasma treatment of the materials increased their hydrophilicity and allowed the adhered BLECs to maintain their normal epithelial morphology. We believe that controlled growth of lens epithelial cells may reduce the incidence of PCO.</abstract><subject lang="en"><genre>keywords</genre><topic>posterior capsular opacification</topic><topic>cellular morphology</topic><topic>gas plasma treatment</topic><topic>surface properties</topic><topic>intraocular lenses</topic></subject><relatedItem type="host"><titleInfo><title>Clinical & Experimental Ophthalmology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1442-6404</identifier><identifier type="eISSN">1442-9071</identifier><identifier type="DOI">10.1111/(ISSN)1442-9071</identifier><identifier type="PublisherID">CEO</identifier><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>34</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>568</start><end>574</end><total>7</total></extent></part></relatedItem><identifier type="istex">C1E093AA3E847852CFDF1DC07AB6981716477330</identifier><identifier type="DOI">10.1111/j.1442-9071.2006.01278.x</identifier><identifier type="ArticleID">CEO1278</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Asia</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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