In vitro adherence of staphylococcus epidermidis to polymethyl methacrylate and acrysof intraocular lenses
Identifieur interne : 003260 ( Istex/Corpus ); précédent : 003259; suivant : 003261In vitro adherence of staphylococcus epidermidis to polymethyl methacrylate and acrysof intraocular lenses
Auteurs : Antonio Pinna ; Stefania Zanetti ; Leonardo A. Sechi ; Donatella Usai ; Maria P. Falchi ; Francesco CartaSource :
- Ophthalmology [ 0161-6420 ] ; 2000.
Abstract
Purpose To investigate the adherence of one clinically relevant ocular isolate of Staphylococcus epidermidis to polymethyl methacrylate (PMMA) and Acrysof (Alcon Surgical, Fort Worth, TX) intraocular lenses (IOLs).Design Experimental study.Participants The authors examined the in vitro adherence of one clinically relevant ocular isolate of S. epidermidis. Adherence was tested on 12 PMMA IOLs and 12 Acrysof IOLs.Methods Six IOLs (three of each type) were placed in different test tubes containing bacterial suspension (108 cfu/ml) and incubated at 37°C. At different times (3 minutes, 30 minutes, and 90 minutes), each IOL type was removed from the test tube, rinsed in sterile phosphate-buffered saline, and transferred into sterile brain-heart infusion broth. The broth with the IOL was sonicated on low power for 3 minutes to remove adhered bacteria. Two serial 10-fold dilutions of the broth containing the dislodged bacteria were plated on mannitol agar plates. The plates were incubated overnight at 37°C and then bacterial colonies were counted. All assays were performed in triplicate. Additional lenses (three of each type) were incubated with S. epidermidis for different times (3 minutes, 30 minutes, and 90 minutes) and then examined with scanning electron microscopy.Main outcome measures The number of adhered bacteria per area (mm2) of IOL optic was calculated. Statistical analysis included calculation of arithmetic means and 95% confidence intervals (t test).Results Direct counting of viable adherent bacteria released by sonication showed that the amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 0.1 × 102/mm2, 3.6 × 102/mm2, and 11 x 102/mm2 (PMMA IOLs), and 4.4 × 102/mm2, 3.1 × 102/mm2, and 2.3 × 102/mm2 (Acrysof IOLs). Direct counting of adherent bacteria in scanning electron microscopy photographs revealed that the total amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 1.1 × 102/mm2, 4.4 × 102/mm2, and 5.5 × 102/mm2 (PMMA IOLs) and 13 × 102/mm2, 33.9 × 102/mm2, and 72 × 102/mm2 (Acrysof IOLs).Conclusions Results suggest that in vitro adherence of S. epidermidis to IOLs is influenced by IOL materials. After 3 minutes’ incubation, Acrysof IOLs appeared to be more permissive to S. epidermidis adherence than PMMA IOLs. The difference was statistically significant (P < 0.05). However, at 90 minutes, Acrysof IOLs had a lower viable bacterial count than did the PMMA IOLs. Bacterial adherence appeared to be greater in areas with surface irregularities. Adherence of S. epidermidis to IOLs may play a role in the pathogenesis of some forms of endophthalmitis after cataract surgery.
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DOI: 10.1016/S0161-6420(00)00080-4
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">In vitro adherence of staphylococcus epidermidis to polymethyl methacrylate and acrysof intraocular lenses</title><author><name sortKey="Pinna, Antonio" sort="Pinna, Antonio" uniqKey="Pinna A" first="Antonio" last="Pinna">Antonio Pinna</name><affiliation><mods:affiliation>Institute of Ophthalmology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Zanetti, Stefania" sort="Zanetti, Stefania" uniqKey="Zanetti S" first="Stefania" last="Zanetti">Stefania Zanetti</name><affiliation><mods:affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Sechi, Leonardo A" sort="Sechi, Leonardo A" uniqKey="Sechi L" first="Leonardo A" last="Sechi">Leonardo A. Sechi</name><affiliation><mods:affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Usai, Donatella" sort="Usai, Donatella" uniqKey="Usai D" first="Donatella" last="Usai">Donatella Usai</name><affiliation><mods:affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Falchi, Maria P" sort="Falchi, Maria P" uniqKey="Falchi M" first="Maria P" last="Falchi">Maria P. Falchi</name><affiliation><mods:affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Carta, Francesco" sort="Carta, Francesco" uniqKey="Carta F" first="Francesco" last="Carta">Francesco Carta</name><affiliation><mods:affiliation>Institute of Ophthalmology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:72C6BBA13FAFB5A132994C7910197D8C36C96BB0</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1016/S0161-6420(00)00080-4</idno><idno type="url">https://api.istex.fr/document/72C6BBA13FAFB5A132994C7910197D8C36C96BB0/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">003260</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">In vitro adherence of staphylococcus epidermidis to polymethyl methacrylate and acrysof intraocular lenses</title><author><name sortKey="Pinna, Antonio" sort="Pinna, Antonio" uniqKey="Pinna A" first="Antonio" last="Pinna">Antonio Pinna</name><affiliation><mods:affiliation>Institute of Ophthalmology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Zanetti, Stefania" sort="Zanetti, Stefania" uniqKey="Zanetti S" first="Stefania" last="Zanetti">Stefania Zanetti</name><affiliation><mods:affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Sechi, Leonardo A" sort="Sechi, Leonardo A" uniqKey="Sechi L" first="Leonardo A" last="Sechi">Leonardo A. Sechi</name><affiliation><mods:affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Usai, Donatella" sort="Usai, Donatella" uniqKey="Usai D" first="Donatella" last="Usai">Donatella Usai</name><affiliation><mods:affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Falchi, Maria P" sort="Falchi, Maria P" uniqKey="Falchi M" first="Maria P" last="Falchi">Maria P. Falchi</name><affiliation><mods:affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Carta, Francesco" sort="Carta, Francesco" uniqKey="Carta F" first="Francesco" last="Carta">Francesco Carta</name><affiliation><mods:affiliation>Institute of Ophthalmology, University of Sassari, Sassari, Italy</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Ophthalmology</title><title level="j" type="abbrev">OPHTHA</title><idno type="ISSN">0161-6420</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">107</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1042">1042</biblScope><biblScope unit="page" to="1046">1046</biblScope></imprint><idno type="ISSN">0161-6420</idno></series><idno type="istex">72C6BBA13FAFB5A132994C7910197D8C36C96BB0</idno><idno type="DOI">10.1016/S0161-6420(00)00080-4</idno><idno type="PII">S0161-6420(00)00080-4</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0161-6420</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Purpose To investigate the adherence of one clinically relevant ocular isolate of Staphylococcus epidermidis to polymethyl methacrylate (PMMA) and Acrysof (Alcon Surgical, Fort Worth, TX) intraocular lenses (IOLs).Design Experimental study.Participants The authors examined the in vitro adherence of one clinically relevant ocular isolate of S. epidermidis. Adherence was tested on 12 PMMA IOLs and 12 Acrysof IOLs.Methods Six IOLs (three of each type) were placed in different test tubes containing bacterial suspension (108 cfu/ml) and incubated at 37°C. At different times (3 minutes, 30 minutes, and 90 minutes), each IOL type was removed from the test tube, rinsed in sterile phosphate-buffered saline, and transferred into sterile brain-heart infusion broth. The broth with the IOL was sonicated on low power for 3 minutes to remove adhered bacteria. Two serial 10-fold dilutions of the broth containing the dislodged bacteria were plated on mannitol agar plates. The plates were incubated overnight at 37°C and then bacterial colonies were counted. All assays were performed in triplicate. Additional lenses (three of each type) were incubated with S. epidermidis for different times (3 minutes, 30 minutes, and 90 minutes) and then examined with scanning electron microscopy.Main outcome measures The number of adhered bacteria per area (mm2) of IOL optic was calculated. Statistical analysis included calculation of arithmetic means and 95% confidence intervals (t test).Results Direct counting of viable adherent bacteria released by sonication showed that the amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 0.1 × 102/mm2, 3.6 × 102/mm2, and 11 x 102/mm2 (PMMA IOLs), and 4.4 × 102/mm2, 3.1 × 102/mm2, and 2.3 × 102/mm2 (Acrysof IOLs). Direct counting of adherent bacteria in scanning electron microscopy photographs revealed that the total amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 1.1 × 102/mm2, 4.4 × 102/mm2, and 5.5 × 102/mm2 (PMMA IOLs) and 13 × 102/mm2, 33.9 × 102/mm2, and 72 × 102/mm2 (Acrysof IOLs).Conclusions Results suggest that in vitro adherence of S. epidermidis to IOLs is influenced by IOL materials. After 3 minutes’ incubation, Acrysof IOLs appeared to be more permissive to S. epidermidis adherence than PMMA IOLs. The difference was statistically significant (P < 0.05). However, at 90 minutes, Acrysof IOLs had a lower viable bacterial count than did the PMMA IOLs. Bacterial adherence appeared to be greater in areas with surface irregularities. Adherence of S. epidermidis to IOLs may play a role in the pathogenesis of some forms of endophthalmitis after cataract surgery.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Antonio Pinna MD</name><affiliations><json:string>Institute of Ophthalmology, University of Sassari, Sassari, Italy</json:string></affiliations></json:item><json:item><name>Stefania Zanetti PhD</name><affiliations><json:string>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</json:string></affiliations></json:item><json:item><name>Leonardo A Sechi PhD</name><affiliations><json:string>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</json:string></affiliations></json:item><json:item><name>Donatella Usai PhD</name><affiliations><json:string>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</json:string></affiliations></json:item><json:item><name>Maria P Falchi PhD</name><affiliations><json:string>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</json:string></affiliations></json:item><json:item><name>Francesco Carta MD</name><affiliations><json:string>Institute of Ophthalmology, University of Sassari, Sassari, Italy</json:string></affiliations></json:item></author><language><json:string>eng</json:string></language><abstract>Purpose To investigate the adherence of one clinically relevant ocular isolate of Staphylococcus epidermidis to polymethyl methacrylate (PMMA) and Acrysof (Alcon Surgical, Fort Worth, TX) intraocular lenses (IOLs).Design Experimental study.Participants The authors examined the in vitro adherence of one clinically relevant ocular isolate of S. epidermidis. Adherence was tested on 12 PMMA IOLs and 12 Acrysof IOLs.Methods Six IOLs (three of each type) were placed in different test tubes containing bacterial suspension (108 cfu/ml) and incubated at 37°C. At different times (3 minutes, 30 minutes, and 90 minutes), each IOL type was removed from the test tube, rinsed in sterile phosphate-buffered saline, and transferred into sterile brain-heart infusion broth. The broth with the IOL was sonicated on low power for 3 minutes to remove adhered bacteria. Two serial 10-fold dilutions of the broth containing the dislodged bacteria were plated on mannitol agar plates. The plates were incubated overnight at 37°C and then bacterial colonies were counted. All assays were performed in triplicate. Additional lenses (three of each type) were incubated with S. epidermidis for different times (3 minutes, 30 minutes, and 90 minutes) and then examined with scanning electron microscopy.Main outcome measures The number of adhered bacteria per area (mm2) of IOL optic was calculated. Statistical analysis included calculation of arithmetic means and 95% confidence intervals (t test).Results Direct counting of viable adherent bacteria released by sonication showed that the amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 0.1 × 102/mm2, 3.6 × 102/mm2, and 11 x 102/mm2 (PMMA IOLs), and 4.4 × 102/mm2, 3.1 × 102/mm2, and 2.3 × 102/mm2 (Acrysof IOLs). Direct counting of adherent bacteria in scanning electron microscopy photographs revealed that the total amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 1.1 × 102/mm2, 4.4 × 102/mm2, and 5.5 × 102/mm2 (PMMA IOLs) and 13 × 102/mm2, 33.9 × 102/mm2, and 72 × 102/mm2 (Acrysof IOLs).Conclusions Results suggest that in vitro adherence of S. epidermidis to IOLs is influenced by IOL materials. After 3 minutes’ incubation, Acrysof IOLs appeared to be more permissive to S. epidermidis adherence than PMMA IOLs. The difference was statistically significant (P > 0.05). However, at 90 minutes, Acrysof IOLs had a lower viable bacterial count than did the PMMA IOLs. Bacterial adherence appeared to be greater in areas with surface irregularities. Adherence of S. epidermidis to IOLs may play a role in the pathogenesis of some forms of endophthalmitis after cataract surgery.</abstract><qualityIndicators><score>5.991</score><pdfVersion>1.2</pdfVersion><pdfPageSize>586 x 786 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>2763</abstractCharCount><pdfWordCount>2991</pdfWordCount><pdfCharCount>19116</pdfCharCount><pdfPageCount>5</pdfPageCount><abstractWordCount>427</abstractWordCount></qualityIndicators><title>In vitro adherence of staphylococcus epidermidis to polymethyl methacrylate and acrysof intraocular lenses</title><pii><json:string>S0161-6420(00)00080-4</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>107</volume><pii><json:string>S0161-6420(00)X0006-1</json:string></pii><pages><last>1046</last><first>1042</first></pages><issn><json:string>0161-6420</json:string></issn><issue>6</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Ophthalmology</title><publicationDate>2000</publicationDate></host><categories><wos><json:string>OPHTHALMOLOGY</json:string></wos></categories><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1016/S0161-6420(00)00080-4</json:string></doi><id>72C6BBA13FAFB5A132994C7910197D8C36C96BB0</id><score>1</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/72C6BBA13FAFB5A132994C7910197D8C36C96BB0/fulltext/pdf</uri></json:item><json:item><original>true</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/72C6BBA13FAFB5A132994C7910197D8C36C96BB0/fulltext/txt</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/72C6BBA13FAFB5A132994C7910197D8C36C96BB0/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/72C6BBA13FAFB5A132994C7910197D8C36C96BB0/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">In vitro adherence of staphylococcus epidermidis to polymethyl methacrylate and acrysof intraocular lenses</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>2000</date></publicationStmt><notesStmt><note>Manuscript no. 99695</note><note>The authors have no financial interest in any material used in this study.</note><note type="content">Figure 1: Scanning electron microscopy photographs: S. epidermidis adherent to the surface of an Acrysof IOL (original magnification, ×2000 [A], ×5000 [B], ×10,000 [C]).</note><note type="content">Figure 2: S. epidermidis adherent to the surface of an Acrysof IOL. The organisms appear to be preferentially colonizing sites with surface irregularities (original magnification, ×1000 [A], ×5000 [B]).</note><note type="content">Figure 3: S. epidermidis adherent to the surface of a PMMA IOL. PMMA IOLs display a smooth-finished surface as shown here. Fewer bacteria are adherent than in Figs 1 and 2. PMMA IOLs appear to have less surface irregularities than Acrysof IOLs (compare with Figs 1 and 2) (original magnification, ×3000 [A], ×10,000 [B]).</note><note type="content">Table 1: Adherence of Staphylococcus epidermidis to PMMA IOLs Determined by Direct Counting of Viable Adherent Bacteria Released by Sonication legend</note><note type="content">Table 2: Adherence of Staphylococcus epidermidis to Acrysof IOLs Determined by Direct Counting of Viable Adherent Bacteria Released by Sonication legend</note><note type="content">Table 3: Adherence of Staphylococcus epidermidis to PMMA IOLs Determined by Direct Counting of Adherent Bacteria in Scanning Electronic Microscopy Photographs legend</note><note type="content">Table 4: Adherence of Staphylococcus epidermidis to Acrysof IOLs Determined by Direct Counting of Adherent Bacteria in Scanning Electronic Microscopy Photographs legend</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">In vitro adherence of staphylococcus epidermidis to polymethyl methacrylate and acrysof intraocular lenses</title><author><persName><forename type="first">Antonio</forename><surname>Pinna</surname></persName><roleName type="degree">MD</roleName><note type="biography">Reprint requests to Antonio Pinna, MD, Institute of Ophthalmology, University of Sassari, Viale San Pietro 43 A, 07100 Sassari, Italy</note><affiliation>Reprint requests to Antonio Pinna, MD, Institute of Ophthalmology, University of Sassari, Viale San Pietro 43 A, 07100 Sassari, Italy</affiliation><affiliation>Institute of Ophthalmology, University of Sassari, Sassari, Italy</affiliation></author><author><persName><forename type="first">Stefania</forename><surname>Zanetti</surname></persName><roleName type="degree">PhD</roleName><affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</affiliation></author><author><persName><forename type="first">Leonardo A</forename><surname>Sechi</surname></persName><roleName type="degree">PhD</roleName><affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</affiliation></author><author><persName><forename type="first">Donatella</forename><surname>Usai</surname></persName><roleName type="degree">PhD</roleName><affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</affiliation></author><author><persName><forename type="first">Maria P</forename><surname>Falchi</surname></persName><roleName type="degree">PhD</roleName><affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</affiliation></author><author><persName><forename type="first">Francesco</forename><surname>Carta</surname></persName><roleName type="degree">MD</roleName><affiliation>Institute of Ophthalmology, University of Sassari, Sassari, Italy</affiliation></author></analytic><monogr><title level="j">Ophthalmology</title><title level="j" type="abbrev">OPHTHA</title><idno type="pISSN">0161-6420</idno><idno type="PII">S0161-6420(00)X0006-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000"></date><biblScope unit="volume">107</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1042">1042</biblScope><biblScope unit="page" to="1046">1046</biblScope></imprint></monogr><idno type="istex">72C6BBA13FAFB5A132994C7910197D8C36C96BB0</idno><idno type="DOI">10.1016/S0161-6420(00)00080-4</idno><idno type="PII">S0161-6420(00)00080-4</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Purpose To investigate the adherence of one clinically relevant ocular isolate of Staphylococcus epidermidis to polymethyl methacrylate (PMMA) and Acrysof (Alcon Surgical, Fort Worth, TX) intraocular lenses (IOLs).Design Experimental study.Participants The authors examined the in vitro adherence of one clinically relevant ocular isolate of S. epidermidis. Adherence was tested on 12 PMMA IOLs and 12 Acrysof IOLs.Methods Six IOLs (three of each type) were placed in different test tubes containing bacterial suspension (108 cfu/ml) and incubated at 37°C. At different times (3 minutes, 30 minutes, and 90 minutes), each IOL type was removed from the test tube, rinsed in sterile phosphate-buffered saline, and transferred into sterile brain-heart infusion broth. The broth with the IOL was sonicated on low power for 3 minutes to remove adhered bacteria. Two serial 10-fold dilutions of the broth containing the dislodged bacteria were plated on mannitol agar plates. The plates were incubated overnight at 37°C and then bacterial colonies were counted. All assays were performed in triplicate. Additional lenses (three of each type) were incubated with S. epidermidis for different times (3 minutes, 30 minutes, and 90 minutes) and then examined with scanning electron microscopy.Main outcome measures The number of adhered bacteria per area (mm2) of IOL optic was calculated. Statistical analysis included calculation of arithmetic means and 95% confidence intervals (t test).Results Direct counting of viable adherent bacteria released by sonication showed that the amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 0.1 × 102/mm2, 3.6 × 102/mm2, and 11 x 102/mm2 (PMMA IOLs), and 4.4 × 102/mm2, 3.1 × 102/mm2, and 2.3 × 102/mm2 (Acrysof IOLs). Direct counting of adherent bacteria in scanning electron microscopy photographs revealed that the total amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 1.1 × 102/mm2, 4.4 × 102/mm2, and 5.5 × 102/mm2 (PMMA IOLs) and 13 × 102/mm2, 33.9 × 102/mm2, and 72 × 102/mm2 (Acrysof IOLs).Conclusions Results suggest that in vitro adherence of S. epidermidis to IOLs is influenced by IOL materials. After 3 minutes’ incubation, Acrysof IOLs appeared to be more permissive to S. epidermidis adherence than PMMA IOLs. The difference was statistically significant (P < 0.05). However, at 90 minutes, Acrysof IOLs had a lower viable bacterial count than did the PMMA IOLs. Bacterial adherence appeared to be greater in areas with surface irregularities. Adherence of S. epidermidis to IOLs may play a role in the pathogenesis of some forms of endophthalmitis after cataract surgery.</p></abstract></profileDesc><revisionDesc><change when="2000-02-15">Registration</change><change when="2000">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="GR1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="GR2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="GR3"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>OPHTHA</jid><aid>262</aid><ce:pii>S0161-6420(00)00080-4</ce:pii><ce:doi>10.1016/S0161-6420(00)00080-4</ce:doi><ce:copyright type="society" year="2000">American Academy of Ophthalmology, Inc.</ce:copyright></item-info><head><ce:title>In vitro adherence of <ce:italic>staphylococcus epidermidis</ce:italic> to polymethyl methacrylate and acrysof intraocular lenses<ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>The authors have no financial interest in any material used in this study.</ce:note-para></ce:footnote></ce:title><ce:presented>Presented in part as a poster at the American Academy of Ophthalmology annual meeting, New Orleans, Louisiana, November 1998.</ce:presented><ce:author-group><ce:author><ce:given-name>Antonio</ce:given-name><ce:surname>Pinna</ce:surname><ce:degrees>MD</ce:degrees><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:author><ce:given-name>Stefania</ce:given-name><ce:surname>Zanetti</ce:surname><ce:degrees>PhD</ce:degrees><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Leonardo A</ce:given-name><ce:surname>Sechi</ce:surname><ce:degrees>PhD</ce:degrees><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Donatella</ce:given-name><ce:surname>Usai</ce:surname><ce:degrees>PhD</ce:degrees><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Maria P</ce:given-name><ce:surname>Falchi</ce:surname><ce:degrees>PhD</ce:degrees><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Francesco</ce:given-name><ce:surname>Carta</ce:surname><ce:degrees>MD</ce:degrees><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>1</ce:label><ce:textfn>Institute of Ophthalmology, University of Sassari, Sassari, Italy</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>2</ce:label><ce:textfn>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Reprint requests to Antonio Pinna, MD, Institute of Ophthalmology, University of Sassari, Viale San Pietro 43 A, 07100 Sassari, Italy</ce:text></ce:correspondence></ce:author-group><ce:date-received day="12" month="10" year="1999"></ce:date-received><ce:date-accepted day="15" month="2" year="2000"></ce:date-accepted><ce:miscellaneous>Manuscript no. 99695</ce:miscellaneous><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:section-title>Purpose</ce:section-title><ce:simple-para>To investigate the adherence of one clinically relevant ocular isolate of <ce:italic>Staphylococcus epidermidis</ce:italic> to polymethyl methacrylate (PMMA) and Acrysof (Alcon Surgical, Fort Worth, TX) intraocular lenses (IOLs).</ce:simple-para></ce:abstract-sec><ce:abstract-sec><ce:section-title>Design</ce:section-title><ce:simple-para>Experimental study.</ce:simple-para></ce:abstract-sec><ce:abstract-sec><ce:section-title>Participants</ce:section-title><ce:simple-para>The authors examined the in vitro adherence of one clinically relevant ocular isolate of <ce:italic>S. epidermidis.</ce:italic> Adherence was tested on 12 PMMA IOLs and 12 Acrysof IOLs.</ce:simple-para></ce:abstract-sec><ce:abstract-sec><ce:section-title>Methods</ce:section-title><ce:simple-para>Six IOLs (three of each type) were placed in different test tubes containing bacterial suspension (10<ce:sup>8</ce:sup> cfu/ml) and incubated at 37°C. At different times (3 minutes, 30 minutes, and 90 minutes), each IOL type was removed from the test tube, rinsed in sterile phosphate-buffered saline, and transferred into sterile brain-heart infusion broth. The broth with the IOL was sonicated on low power for 3 minutes to remove adhered bacteria. Two serial 10-fold dilutions of the broth containing the dislodged bacteria were plated on mannitol agar plates. The plates were incubated overnight at 37°C and then bacterial colonies were counted. All assays were performed in triplicate. Additional lenses (three of each type) were incubated with <ce:italic>S. epidermidis</ce:italic> for different times (3 minutes, 30 minutes, and 90 minutes) and then examined with scanning electron microscopy.</ce:simple-para></ce:abstract-sec><ce:abstract-sec><ce:section-title>Main outcome measures</ce:section-title><ce:simple-para>The number of adhered bacteria per area (mm<ce:sup>2</ce:sup>) of IOL optic was calculated. Statistical analysis included calculation of arithmetic means and 95% confidence intervals (<ce:italic>t</ce:italic> test).</ce:simple-para></ce:abstract-sec><ce:abstract-sec><ce:section-title>Results</ce:section-title><ce:simple-para>Direct counting of viable adherent bacteria released by sonication showed that the amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in <ce:italic>S. epidermidis</ce:italic> suspension was 0.1 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup>, 3.6 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup>, and 11 x 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup> (PMMA IOLs), and 4.4 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup>, 3.1 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup>, and 2.3 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup> (Acrysof IOLs). Direct counting of adherent bacteria in scanning electron microscopy photographs revealed that the total amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in <ce:italic>S. epidermidis</ce:italic> suspension was 1.1 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup>, 4.4 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup>, and 5.5 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup> (PMMA IOLs) and 13 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup>, 33.9 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup>, and 72 × 10<ce:sup>2</ce:sup>/mm<ce:sup>2</ce:sup> (Acrysof IOLs).</ce:simple-para></ce:abstract-sec><ce:abstract-sec><ce:section-title>Conclusions</ce:section-title><ce:simple-para>Results suggest that in vitro adherence of <ce:italic>S. epidermidis</ce:italic> to IOLs is influenced by IOL materials. After 3 minutes’ incubation, Acrysof IOLs appeared to be more permissive to <ce:italic>S. epidermidis</ce:italic> adherence than PMMA IOLs. The difference was statistically significant (<ce:italic>P</ce:italic> < 0.05). However, at 90 minutes, Acrysof IOLs had a lower viable bacterial count than did the PMMA IOLs. Bacterial adherence appeared to be greater in areas with surface irregularities. Adherence of <ce:italic>S. epidermidis</ce:italic> to IOLs may play a role in the pathogenesis of some forms of endophthalmitis after cataract surgery.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>In vitro adherence of staphylococcus epidermidis to polymethyl methacrylate and acrysof intraocular lenses</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>In vitro adherence of</title></titleInfo><name type="personal"><namePart type="given">Antonio</namePart><namePart type="family">Pinna</namePart><namePart type="termsOfAddress">MD</namePart><affiliation>Institute of Ophthalmology, University of Sassari, Sassari, Italy</affiliation><description>Reprint requests to Antonio Pinna, MD, Institute of Ophthalmology, University of Sassari, Viale San Pietro 43 A, 07100 Sassari, Italy</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stefania</namePart><namePart type="family">Zanetti</namePart><namePart type="termsOfAddress">PhD</namePart><affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Leonardo A</namePart><namePart type="family">Sechi</namePart><namePart type="termsOfAddress">PhD</namePart><affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Donatella</namePart><namePart type="family">Usai</namePart><namePart type="termsOfAddress">PhD</namePart><affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Maria P</namePart><namePart type="family">Falchi</namePart><namePart type="termsOfAddress">PhD</namePart><affiliation>Department of Biomedical Sciences, Section of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Francesco</namePart><namePart type="family">Carta</namePart><namePart type="termsOfAddress">MD</namePart><affiliation>Institute of Ophthalmology, University of Sassari, Sassari, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued><dateValid encoding="w3cdtf">2000-02-15</dateValid><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Purpose To investigate the adherence of one clinically relevant ocular isolate of Staphylococcus epidermidis to polymethyl methacrylate (PMMA) and Acrysof (Alcon Surgical, Fort Worth, TX) intraocular lenses (IOLs).Design Experimental study.Participants The authors examined the in vitro adherence of one clinically relevant ocular isolate of S. epidermidis. Adherence was tested on 12 PMMA IOLs and 12 Acrysof IOLs.Methods Six IOLs (three of each type) were placed in different test tubes containing bacterial suspension (108 cfu/ml) and incubated at 37°C. At different times (3 minutes, 30 minutes, and 90 minutes), each IOL type was removed from the test tube, rinsed in sterile phosphate-buffered saline, and transferred into sterile brain-heart infusion broth. The broth with the IOL was sonicated on low power for 3 minutes to remove adhered bacteria. Two serial 10-fold dilutions of the broth containing the dislodged bacteria were plated on mannitol agar plates. The plates were incubated overnight at 37°C and then bacterial colonies were counted. All assays were performed in triplicate. Additional lenses (three of each type) were incubated with S. epidermidis for different times (3 minutes, 30 minutes, and 90 minutes) and then examined with scanning electron microscopy.Main outcome measures The number of adhered bacteria per area (mm2) of IOL optic was calculated. Statistical analysis included calculation of arithmetic means and 95% confidence intervals (t test).Results Direct counting of viable adherent bacteria released by sonication showed that the amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 0.1 × 102/mm2, 3.6 × 102/mm2, and 11 x 102/mm2 (PMMA IOLs), and 4.4 × 102/mm2, 3.1 × 102/mm2, and 2.3 × 102/mm2 (Acrysof IOLs). Direct counting of adherent bacteria in scanning electron microscopy photographs revealed that the total amount of adhered bacteria per area of IOL optic after 3, 30, and 90 minutes’ incubation in S. epidermidis suspension was 1.1 × 102/mm2, 4.4 × 102/mm2, and 5.5 × 102/mm2 (PMMA IOLs) and 13 × 102/mm2, 33.9 × 102/mm2, and 72 × 102/mm2 (Acrysof IOLs).Conclusions Results suggest that in vitro adherence of S. epidermidis to IOLs is influenced by IOL materials. After 3 minutes’ incubation, Acrysof IOLs appeared to be more permissive to S. epidermidis adherence than PMMA IOLs. The difference was statistically significant (P < 0.05). However, at 90 minutes, Acrysof IOLs had a lower viable bacterial count than did the PMMA IOLs. Bacterial adherence appeared to be greater in areas with surface irregularities. Adherence of S. epidermidis to IOLs may play a role in the pathogenesis of some forms of endophthalmitis after cataract surgery.</abstract><note>Manuscript no. 99695</note><note type="footnote">The authors have no financial interest in any material used in this study.</note><note type="content">Figure 1: Scanning electron microscopy photographs: S. epidermidis adherent to the surface of an Acrysof IOL (original magnification, ×2000 [A], ×5000 [B], ×10,000 [C]).</note><note type="content">Figure 2: S. epidermidis adherent to the surface of an Acrysof IOL. The organisms appear to be preferentially colonizing sites with surface irregularities (original magnification, ×1000 [A], ×5000 [B]).</note><note type="content">Figure 3: S. epidermidis adherent to the surface of a PMMA IOL. PMMA IOLs display a smooth-finished surface as shown here. Fewer bacteria are adherent than in Figs 1 and 2. PMMA IOLs appear to have less surface irregularities than Acrysof IOLs (compare with Figs 1 and 2) (original magnification, ×3000 [A], ×10,000 [B]).</note><note type="content">Table 1: Adherence of Staphylococcus epidermidis to PMMA IOLs Determined by Direct Counting of Viable Adherent Bacteria Released by Sonication legend</note><note type="content">Table 2: Adherence of Staphylococcus epidermidis to Acrysof IOLs Determined by Direct Counting of Viable Adherent Bacteria Released by Sonication legend</note><note type="content">Table 3: Adherence of Staphylococcus epidermidis to PMMA IOLs Determined by Direct Counting of Adherent Bacteria in Scanning Electronic Microscopy Photographs legend</note><note type="content">Table 4: Adherence of Staphylococcus epidermidis to Acrysof IOLs Determined by Direct Counting of Adherent Bacteria in Scanning Electronic Microscopy Photographs legend</note><relatedItem type="host"><titleInfo><title>Ophthalmology</title></titleInfo><titleInfo type="abbreviated"><title>OPHTHA</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">200006</dateIssued></originInfo><identifier type="ISSN">0161-6420</identifier><identifier type="PII">S0161-6420(00)X0006-1</identifier><part><date>200006</date><detail type="volume"><number>107</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="issue pages"><start>A1</start><end>A48</end></extent><extent unit="issue pages"><start>1019</start><end>1210</end></extent><extent unit="pages"><start>1042</start><end>1046</end></extent></part></relatedItem><identifier type="istex">72C6BBA13FAFB5A132994C7910197D8C36C96BB0</identifier><identifier type="DOI">10.1016/S0161-6420(00)00080-4</identifier><identifier type="PII">S0161-6420(00)00080-4</identifier><accessCondition type="use and reproduction" contentType="">© 2000American Academy of Ophthalmology, Inc.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>American Academy of Ophthalmology, Inc., ©2000</recordOrigin></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/72C6BBA13FAFB5A132994C7910197D8C36C96BB0/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">OPHTHALMOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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