In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt
Identifieur interne : 003091 ( Istex/Corpus ); précédent : 003090; suivant : 003092In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt
Auteurs : Xiao Wei Tan ; Andri Riau ; Zhi Long Shi ; Anna C S. Tan ; Koon Gee Neoh ; Khiam Aik Khor ; Roger W. Beuerman ; Donald Tan ; Jodhbir S. MehtaSource :
- British Journal of Ophthalmology [ 0007-1161 ] ; 2012-09.
Abstract
Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.
Url:
DOI: 10.1136/bjophthalmol-2012-301633
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<front><div type="abstract">Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</div>
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<abstract>Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</abstract>
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<abstract><p>Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</p>
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<journal-title>British Journal of Ophthalmology</journal-title>
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<title-group><article-title>In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Tan</surname>
<given-names>Xiao Wei</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Riau</surname>
<given-names>Andri</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Shi</surname>
<given-names>Zhi Long</given-names>
</name>
<xref ref-type="aff" rid="aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tan</surname>
<given-names>Anna C S</given-names>
</name>
<xref ref-type="aff" rid="aff3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Neoh</surname>
<given-names>Koon Gee</given-names>
</name>
<xref ref-type="aff" rid="aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Khor</surname>
<given-names>Khiam Aik</given-names>
</name>
<xref ref-type="aff" rid="aff4">4</xref>
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<contrib contrib-type="author"><name><surname>Beuerman</surname>
<given-names>Roger W</given-names>
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<xref ref-type="aff" rid="aff5">5</xref>
<xref ref-type="aff" rid="aff6">6</xref>
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<contrib contrib-type="author"><name><surname>Tan</surname>
<given-names>Donald</given-names>
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<xref ref-type="aff" rid="aff2">2</xref>
<xref ref-type="aff" rid="aff3">3</xref>
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<contrib contrib-type="author"><name><surname>Mehta</surname>
<given-names>Jodhbir S</given-names>
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<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff3">3</xref>
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<aff id="aff1"><label>1</label>
Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</aff>
<aff id="aff2"><label>2</label>
School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</aff>
<aff id="aff3"><label>3</label>
Singapore National Eye Centre, Singapore</aff>
<aff id="aff4"><label>4</label>
School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore</aff>
<aff id="aff5"><label>5</label>
Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</aff>
<aff id="aff6"><label>6</label>
Duke-NUS SRP Neuroscience and Behavioral Disorders, Singapore</aff>
<aff id="aff7"><label>7</label>
Department of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore</aff>
<author-notes><corresp><label>Correspondence to</label>
Professor Jodhbir S Mehta, Singapore Eye Research Institute, 11 Third Hospital Drive, Singapore 168751, Singapore; <email>jodmehta@gmail.com</email>
</corresp>
<fn fn-type="other"><p>Additional materials are published online only. To view these files please visit the journal online (<ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1136/bjophthalmol-2012-301633">http://dx.doi.org/10.1136/bjophthalmol-2012-301633</ext-link>
).</p>
</fn>
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<pub-date pub-type="ppub"><month>9</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="epub"><day>16</day>
<month>7</month>
<year>2012</year>
</pub-date>
<volume>96</volume>
<volume-id pub-id-type="other">96</volume-id>
<volume-id pub-id-type="other">96</volume-id>
<issue>9</issue>
<issue-id pub-id-type="other">bjophthalmol;96/9</issue-id>
<issue-id pub-id-type="other">9</issue-id>
<issue-id pub-id-type="other">96/9</issue-id>
<fpage>1252</fpage>
<history><date date-type="accepted"><day>16</day>
<month>6</month>
<year>2012</year>
</date>
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<permissions><copyright-statement>© 2012, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</copyright-statement>
<copyright-year>2012</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/"><p>This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/">http://creativecommons.org/licenses/by-nc/2.0/</ext-link>
and <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/legalcode">http://creativecommons.org/licenses/by-nc/2.0/legalcode</ext-link>
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<abstract><sec><title>Aim</title>
<p>Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO<sub>2</sub>
) in a simulated in vitro cornea inflammation environment.</p>
</sec>
<sec><title>Methods</title>
<p>Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO<sub>2</sub>
. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy.</p>
</sec>
<sec><title>Results</title>
<p>There were less cytokines secreted from human corneal fibroblasts seeded on TiO<sub>2</sub>
substrates as compared with HA. TiO<sub>2</sub>
was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO<sub>2</sub>
was more stable than HA after long-term incubation with ATF.</p>
</sec>
<sec><title>Conclusions</title>
<p>TiO<sub>2</sub>
is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</p>
</sec>
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<abstract>Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</abstract>
<note type="footnotes">Additional materials are published online only. To view these files please visit the journal online (http://dx.doi.org/10.1136/bjophthalmol-2012-301633).</note>
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<topic>OOKP</topic>
<topic>microbial infection</topic>
<topic>material dissolution</topic>
<topic>artificial tear fluid</topic>
<topic>cornea</topic>
<topic>biochemistry</topic>
<topic>prosthesis</topic>
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<topic>stem cells</topic>
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