In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt
Identifieur interne : 003091 ( Istex/Corpus ); précédent : 003090; suivant : 003092In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt
Auteurs : Xiao Wei Tan ; Andri Riau ; Zhi Long Shi ; Anna C S. Tan ; Koon Gee Neoh ; Khiam Aik Khor ; Roger W. Beuerman ; Donald Tan ; Jodhbir S. MehtaSource :
- British Journal of Ophthalmology [ 0007-1161 ] ; 2012-09.
Abstract
Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.
Url:
DOI: 10.1136/bjophthalmol-2012-301633
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Tan</name><affiliation><mods:affiliation>Singapore National Eye Centre, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Neoh, Koon Gee" sort="Neoh, Koon Gee" uniqKey="Neoh K" first="Koon Gee" last="Neoh">Koon Gee Neoh</name><affiliation><mods:affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Khor, Khiam Aik" sort="Khor, Khiam Aik" uniqKey="Khor K" first="Khiam Aik" last="Khor">Khiam Aik Khor</name><affiliation><mods:affiliation>School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Beuerman, Roger W" sort="Beuerman, Roger W" uniqKey="Beuerman R" first="Roger W" last="Beuerman">Roger W. Beuerman</name><affiliation><mods:affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>Duke-NUS SRP Neuroscience and Behavioral Disorders, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Tan, Donald" sort="Tan, Donald" uniqKey="Tan D" first="Donald" last="Tan">Donald Tan</name><affiliation><mods:affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>Singapore National Eye Centre, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Mehta, Jodhbir S" sort="Mehta, Jodhbir S" uniqKey="Mehta J" first="Jodhbir S" last="Mehta">Jodhbir S. 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Tan</name><affiliation><mods:affiliation>Singapore National Eye Centre, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Neoh, Koon Gee" sort="Neoh, Koon Gee" uniqKey="Neoh K" first="Koon Gee" last="Neoh">Koon Gee Neoh</name><affiliation><mods:affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Khor, Khiam Aik" sort="Khor, Khiam Aik" uniqKey="Khor K" first="Khiam Aik" last="Khor">Khiam Aik Khor</name><affiliation><mods:affiliation>School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Beuerman, Roger W" sort="Beuerman, Roger W" uniqKey="Beuerman R" first="Roger W" last="Beuerman">Roger W. Beuerman</name><affiliation><mods:affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>Duke-NUS SRP Neuroscience and Behavioral Disorders, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Tan, Donald" sort="Tan, Donald" uniqKey="Tan D" first="Donald" last="Tan">Donald Tan</name><affiliation><mods:affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>Singapore National Eye Centre, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Mehta, Jodhbir S" sort="Mehta, Jodhbir S" uniqKey="Mehta J" first="Jodhbir S" last="Mehta">Jodhbir S. Mehta</name><affiliation><mods:affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>Singapore National Eye Centre, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">British Journal of Ophthalmology</title><title level="j" type="abbrev">Br J Ophthalmol</title><idno type="ISSN">0007-1161</idno><idno type="eISSN">1468-2079</idno><imprint><publisher>BMJ Publishing Group Ltd.</publisher><pubPlace>BMA House, Tavistock Square, London, WC1H 9JR</pubPlace><date type="published" when="2012-09">2012-09</date><biblScope unit="volume">96</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="1252">1252</biblScope></imprint><idno type="ISSN">0007-1161</idno></series><idno type="istex">17555A97CEBD6DC260CE6D7CF8DF71BDDF82C538</idno><idno type="DOI">10.1136/bjophthalmol-2012-301633</idno><idno type="href">bjophthalmol-96-1252.pdf</idno><idno type="ArticleID">bjophthalmol-2012-301633</idno><idno type="PMID">22802307</idno><idno type="local">bjophthalmol;96/9/1252</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0007-1161</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</div></front></TEI><istex><corpusName>bmj</corpusName><author><json:item><name>Xiao Wei Tan</name><affiliations><json:string>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</json:string></affiliations></json:item><json:item><name>Andri Riau</name><affiliations><json:string>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</json:string></affiliations></json:item><json:item><name>Zhi Long Shi</name><affiliations><json:string>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</json:string></affiliations></json:item><json:item><name>Anna C S Tan</name><affiliations><json:string>Singapore National Eye Centre, Singapore</json:string></affiliations></json:item><json:item><name>Koon Gee Neoh</name><affiliations><json:string>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</json:string></affiliations></json:item><json:item><name>Khiam Aik Khor</name><affiliations><json:string>School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore</json:string></affiliations></json:item><json:item><name>Roger W Beuerman</name><affiliations><json:string>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</json:string><json:string>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</json:string><json:string>Duke-NUS SRP Neuroscience and Behavioral Disorders, Singapore</json:string></affiliations></json:item><json:item><name>Donald Tan</name><affiliations><json:string>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</json:string><json:string>Singapore National Eye Centre, Singapore</json:string></affiliations></json:item><json:item><name>Jodhbir S Mehta</name><affiliations><json:string>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</json:string><json:string>Singapore National Eye Centre, Singapore</json:string><json:string>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</json:string><json:string>Department of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Open access</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>OOKP</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>microbial infection</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>material dissolution</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>artificial tear fluid</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cornea</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>biochemistry</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>prosthesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>microbiology</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>contact lens</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>stem cells</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>lens and zonules</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>treatment surgery</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>epidemiology</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>experimental and animal models</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ocular surface</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>genetics</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>imaging</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>treatment lasers</value></json:item></subject><articleId><json:string>bjophthalmol-2012-301633</json:string></articleId><language><json:string>eng</json:string></language><abstract>Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</abstract><qualityIndicators><score>5.905</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.163 x 793.587 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>19</keywordCount><abstractCharCount>1304</abstractCharCount><pdfWordCount>3685</pdfWordCount><pdfCharCount>24022</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>185</abstractWordCount></qualityIndicators><title>In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt</title><pmid><json:string>22802307</json:string></pmid><genre.original><json:string>research-article</json:string></genre.original><genre><json:string>research-article</json:string></genre><host><volume>96</volume><publisherId><json:string>bjo</json:string></publisherId><pages><first>1252</first></pages><issn><json:string>0007-1161</json:string></issn><issue>9</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1468-2079</json:string></eissn><title>British Journal of Ophthalmology</title></host><publicationDate>2012</publicationDate><copyrightDate>2012</copyrightDate><doi><json:string>10.1136/bjophthalmol-2012-301633</json:string></doi><id>17555A97CEBD6DC260CE6D7CF8DF71BDDF82C538</id><score>1</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/17555A97CEBD6DC260CE6D7CF8DF71BDDF82C538/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/17555A97CEBD6DC260CE6D7CF8DF71BDDF82C538/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/17555A97CEBD6DC260CE6D7CF8DF71BDDF82C538/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>BMJ Publishing Group Ltd.</publisher><pubPlace>BMA House, Tavistock Square, London, WC1H 9JR</pubPlace><availability><p>BMJ</p></availability><date>2012-07-16</date></publicationStmt><notesStmt><note>Additional materials are published online only. To view these files please visit the journal online (http://dx.doi.org/10.1136/bjophthalmol-2012-301633).</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt</title><author><persName><forename type="first">Xiao Wei</forename><surname>Tan</surname></persName><affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</affiliation></author><author><persName><forename type="first">Andri</forename><surname>Riau</surname></persName><affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</affiliation></author><author><persName><forename type="first">Zhi Long</forename><surname>Shi</surname></persName><affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</affiliation></author><author><persName><forename type="first">Anna C S</forename><surname>Tan</surname></persName><affiliation>Singapore National Eye Centre, Singapore</affiliation></author><author><persName><forename type="first">Koon Gee</forename><surname>Neoh</surname></persName><affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</affiliation></author><author><persName><forename type="first">Khiam Aik</forename><surname>Khor</surname></persName><affiliation>School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore</affiliation></author><author><persName><forename type="first">Roger W</forename><surname>Beuerman</surname></persName><affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</affiliation><affiliation>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</affiliation><affiliation>Duke-NUS SRP Neuroscience and Behavioral Disorders, Singapore</affiliation></author><author><persName><forename type="first">Donald</forename><surname>Tan</surname></persName><affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</affiliation><affiliation>Singapore National Eye Centre, Singapore</affiliation></author><author><persName><forename type="first">Jodhbir S</forename><surname>Mehta</surname></persName><affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</affiliation><affiliation>Singapore National Eye Centre, Singapore</affiliation><affiliation>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</affiliation><affiliation>Department of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore</affiliation></author></analytic><monogr><title level="j">British Journal of Ophthalmology</title><title level="j" type="abbrev">Br J Ophthalmol</title><idno type="pISSN">0007-1161</idno><idno type="eISSN">1468-2079</idno><imprint><publisher>BMJ Publishing Group Ltd.</publisher><pubPlace>BMA House, Tavistock Square, London, WC1H 9JR</pubPlace><date type="published" when="2012-09"></date><biblScope unit="volume">96</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="1252">1252</biblScope></imprint></monogr><idno type="istex">17555A97CEBD6DC260CE6D7CF8DF71BDDF82C538</idno><idno type="DOI">10.1136/bjophthalmol-2012-301633</idno><idno type="href">bjophthalmol-96-1252.pdf</idno><idno type="ArticleID">bjophthalmol-2012-301633</idno><idno type="PMID">22802307</idno><idno type="local">bjophthalmol;96/9/1252</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-07-16</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</p></abstract><textClass><keywords scheme="keyword"><list><head>hwp-journal-coll</head><item><term>Open access</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>OOKP</term></item><item><term>microbial infection</term></item><item><term>material dissolution</term></item><item><term>artificial tear fluid</term></item><item><term>cornea</term></item><item><term>biochemistry</term></item><item><term>prosthesis</term></item><item><term>microbiology</term></item><item><term>contact lens</term></item><item><term>stem cells</term></item><item><term>lens and zonules</term></item><item><term>treatment surgery</term></item><item><term>epidemiology</term></item><item><term>experimental and animal models</term></item><item><term>ocular surface</term></item><item><term>genetics</term></item><item><term>imaging</term></item><item><term>treatment 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journal-id-type="publisher-id">bjo</journal-id><journal-title>British Journal of Ophthalmology</journal-title><abbrev-journal-title abbrev-type="publisher">Br J Ophthalmol</abbrev-journal-title><abbrev-journal-title>Br J Ophthalmol</abbrev-journal-title><issn pub-type="ppub">0007-1161</issn><issn pub-type="epub">1468-2079</issn><publisher><publisher-name>BMJ Publishing Group Ltd.</publisher-name><publisher-loc>BMA House, Tavistock Square, London, WC1H 9JR</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">bjophthalmol-2012-301633</article-id><article-id pub-id-type="doi">10.1136/bjophthalmol-2012-301633</article-id><article-id pub-id-type="other">bjophthalmol;96/9/1252</article-id><article-id pub-id-type="other">bjophthalmol;bjophthalmol-2012-301633</article-id><article-id pub-id-type="pmid">22802307</article-id><article-id pub-id-type="other">1252</article-id><article-id pub-id-type="other">bjophthalmol-2012-301633</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original articles</subject></subj-group><subj-group subj-group-type="hwp-journal-coll"><subject>Open access</subject></subj-group><series-title>Laboratory science</series-title></article-categories><title-group><article-title>In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Tan</surname><given-names>Xiao Wei</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Riau</surname><given-names>Andri</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Shi</surname><given-names>Zhi Long</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Tan</surname><given-names>Anna C S</given-names></name><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Neoh</surname><given-names>Koon Gee</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Khor</surname><given-names>Khiam Aik</given-names></name><xref ref-type="aff" rid="aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Beuerman</surname><given-names>Roger W</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff5">5</xref><xref ref-type="aff" rid="aff6">6</xref></contrib><contrib contrib-type="author"><name><surname>Tan</surname><given-names>Donald</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Mehta</surname><given-names>Jodhbir S</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff3">3</xref><xref ref-type="aff" rid="aff5">5</xref><xref ref-type="aff" rid="aff7">7</xref></contrib></contrib-group><aff id="aff1"><label>1</label>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</aff><aff id="aff2"><label>2</label>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</aff><aff id="aff3"><label>3</label>Singapore National Eye Centre, Singapore</aff><aff id="aff4"><label>4</label>School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore</aff><aff id="aff5"><label>5</label>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</aff><aff id="aff6"><label>6</label>Duke-NUS SRP Neuroscience and Behavioral Disorders, Singapore</aff><aff id="aff7"><label>7</label>Department of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore</aff><author-notes><corresp><label>Correspondence to</label> Professor Jodhbir S Mehta, Singapore Eye Research Institute, 11 Third Hospital Drive, Singapore 168751, Singapore; <email>jodmehta@gmail.com</email></corresp><fn fn-type="other"><p>Additional materials are published online only. To view these files please visit the journal online (<ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1136/bjophthalmol-2012-301633">http://dx.doi.org/10.1136/bjophthalmol-2012-301633</ext-link>).</p></fn></author-notes><pub-date pub-type="ppub"><month>9</month><year>2012</year></pub-date><pub-date pub-type="epub"><day>16</day><month>7</month><year>2012</year></pub-date><volume>96</volume><volume-id pub-id-type="other">96</volume-id><volume-id pub-id-type="other">96</volume-id><issue>9</issue><issue-id pub-id-type="other">bjophthalmol;96/9</issue-id><issue-id pub-id-type="other">9</issue-id><issue-id pub-id-type="other">96/9</issue-id><fpage>1252</fpage><history><date date-type="accepted"><day>16</day><month>6</month><year>2012</year></date></history><permissions><copyright-statement>© 2012, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</copyright-statement><copyright-year>2012</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/"><p>This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/">http://creativecommons.org/licenses/by-nc/2.0/</ext-link> and <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/legalcode">http://creativecommons.org/licenses/by-nc/2.0/legalcode</ext-link>.</p></license></permissions><self-uri content-type="pdf" xlink:role="full-text" xlink:href="bjophthalmol-96-1252.pdf"></self-uri><abstract><sec><title>Aim</title><p>Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO<sub>2</sub>) in a simulated in vitro cornea inflammation environment.</p></sec><sec><title>Methods</title><p>Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO<sub>2</sub>. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy.</p></sec><sec><title>Results</title><p>There were less cytokines secreted from human corneal fibroblasts seeded on TiO<sub>2</sub> substrates as compared with HA. TiO<sub>2</sub> was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO<sub>2</sub> was more stable than HA after long-term incubation with ATF.</p></sec><sec><title>Conclusions</title><p>TiO<sub>2</sub> is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</p></sec></abstract><kwd-group><kwd>OOKP</kwd><kwd>microbial infection</kwd><kwd>material dissolution</kwd><kwd>artificial tear fluid</kwd><kwd>cornea</kwd><kwd>biochemistry</kwd><kwd>prosthesis</kwd><kwd>microbiology</kwd><kwd>contact lens</kwd><kwd>stem cells</kwd><kwd>lens and zonules</kwd><kwd>treatment surgery</kwd><kwd>epidemiology</kwd><kwd>experimental and animal models</kwd><kwd>ocular surface</kwd><kwd>genetics</kwd><kwd>imaging</kwd><kwd>treatment lasers</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><partName>Laboratory science</partName><title>In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><partName>Laboratory science</partName><title>In vitro effect of a corrosive hostile ocular surface on candidate biomaterials for keratoprosthesis skirt</title></titleInfo><name type="personal"><namePart type="given">Xiao Wei</namePart><namePart type="family">Tan</namePart><affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Andri</namePart><namePart type="family">Riau</namePart><affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zhi Long</namePart><namePart type="family">Shi</namePart><affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Anna C S</namePart><namePart type="family">Tan</namePart><affiliation>Singapore National Eye Centre, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Koon Gee</namePart><namePart type="family">Neoh</namePart><affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Khiam Aik</namePart><namePart type="family">Khor</namePart><affiliation>School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Roger W</namePart><namePart type="family">Beuerman</namePart><affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</affiliation><affiliation>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</affiliation><affiliation>Duke-NUS SRP Neuroscience and Behavioral Disorders, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Donald</namePart><namePart type="family">Tan</namePart><affiliation>School of Chemical and Bimolecular Engineering, National University of Singapore, Singapore</affiliation><affiliation>Singapore National Eye Centre, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jodhbir S</namePart><namePart type="family">Mehta</namePart><affiliation>Tissue Engineering and Stem Cell Research Group, Singapore Eye Research Institute, Singapore</affiliation><affiliation>Singapore National Eye Centre, Singapore</affiliation><affiliation>Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</affiliation><affiliation>Department of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><subject><genre>hwp-journal-coll</genre><topic>Open access</topic></subject><originInfo><publisher>BMJ Publishing Group Ltd.</publisher><place><placeTerm type="text">BMA House, Tavistock Square, London, WC1H 9JR</placeTerm></place><dateIssued encoding="w3cdtf">2012-09</dateIssued><dateCreated encoding="w3cdtf">2012-07-16</dateCreated><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Aim Keratoprosthesis (KPro) devices are prone to long-term corrosion and microbiological assault. The authors aimed to compare the inflammatory response and material dissolution properties of two candidate KPro skirt materials, hydroxyapatite (HA) and titania (TiO2) in a simulated in vitro cornea inflammation environment. Methods Lipopolysaccharide-stimulated cytokine secretions were evaluated with human corneal fibroblasts on both HA and TiO2. Material specimens were subjected to electrochemical and long-term incubation test with artificial tear fluid (ATF) of various acidities. Topography and surface roughness of material discs were analysed by scanning electron microscopy and atomic force microscopy. Results There were less cytokines secreted from human corneal fibroblasts seeded on TiO2 substrates as compared with HA. TiO2 was more resistant to the corrosion effect caused by acidic ATF in contrast to HA. Moreover, the elemental composition of TiO2 was more stable than HA after long-term incubation with ATF. Conclusions TiO2 is more resistant to inflammatory degradation and has a higher corrosion resistance as compared with HA, and in this regard may be a suitable material to replace HA as an osteo-odonto-keratoprosthesis skirt. This would reduce resorption rates for KPro surgery.</abstract><note type="footnotes">Additional materials are published online only. To view these files please visit the journal online (http://dx.doi.org/10.1136/bjophthalmol-2012-301633).</note><subject><genre>Keywords</genre><topic>OOKP</topic><topic>microbial infection</topic><topic>material dissolution</topic><topic>artificial tear fluid</topic><topic>cornea</topic><topic>biochemistry</topic><topic>prosthesis</topic><topic>microbiology</topic><topic>contact lens</topic><topic>stem cells</topic><topic>lens and zonules</topic><topic>treatment surgery</topic><topic>epidemiology</topic><topic>experimental and animal models</topic><topic>ocular surface</topic><topic>genetics</topic><topic>imaging</topic><topic>treatment lasers</topic></subject><relatedItem type="host"><titleInfo><title>British Journal of Ophthalmology</title></titleInfo><titleInfo type="abbreviated"><title>Br J Ophthalmol</title></titleInfo><genre type="Journal">journal</genre><identifier type="ISSN">0007-1161</identifier><identifier type="eISSN">1468-2079</identifier><identifier type="PublisherID">bjo</identifier><identifier type="PublisherID-hwp">bjophthalmol</identifier><identifier type="PublisherID-nlm-ta">Br J Ophthalmol</identifier><part><date>2012</date><detail type="volume"><caption>vol.</caption><number>96</number></detail><detail type="issue"><caption>no.</caption><number>9</number></detail><extent unit="pages"><start>1252</start></extent></part></relatedItem><identifier type="istex">17555A97CEBD6DC260CE6D7CF8DF71BDDF82C538</identifier><identifier type="DOI">10.1136/bjophthalmol-2012-301633</identifier><identifier type="href">bjophthalmol-96-1252.pdf</identifier><identifier type="ArticleID">bjophthalmol-2012-301633</identifier><identifier type="PMID">22802307</identifier><identifier type="local">bjophthalmol;96/9/1252</identifier><accessCondition type="use and reproduction" contentType="open-access">This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.</accessCondition><recordInfo><recordContentSource>BMJ</recordContentSource></recordInfo></mods></metadata><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/17555A97CEBD6DC260CE6D7CF8DF71BDDF82C538/annexes/jpeg</uri></json:item></annexes><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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