A high-throughput standing surface acoustic wave (SSAW)-based cell sorter
Identifieur interne : 000428 ( Pmc/Corpus ); précédent : 000427; suivant : 000429A high-throughput standing surface acoustic wave (SSAW)-based cell sorter
Auteurs : Liqiang Ren ; Yuchao Chen ; Peng Li ; Zhangming Mao ; Po-Hsun Huang ; Joseph Rufo ; Feng Guo ; Lin Wang ; J. Philip Mccoy ; Stewart J. Levine ; Tony Jun HuangSource :
- Lab on a chip [ 1473-0197 ] ; 2015.
Abstract
Acoustic-based fluorescence activated cell sorters (FACS) have drawn increased attention in recent years due to their versatility, high biocompatibility, high controllability, and simple design. However, the sorting throughput for existing acoustic cell sorters is far from optimum for practical applications. Here we report a high-throughput cell sorting method based on standing surface acoustic waves (SSAWs). We utilized a pair of focused interdigital transducers (FIDTs) to generate SSAW with high resolution and high energy efficiency. As a result, the sorting throughput is improved significantly from conventional acoustic-based cell sorting methods. We demonstrated the successful sorting of 10 μm polystyrene particles with a minimum actuation time of 72 μs, which translates to a potential sorting rate of more than 13,800 events/s. Without using a cell-detection unit, we were able to demonstrate an actual sorting throughput of 3,300 events/s. Our sorting method can be conveniently integrated with upstream detection units, and it represents an important development towards a functional acoustic-based FACS system.
Url:
DOI: 10.1039/c5lc00706b
PubMed: 26289231
PubMed Central: 4641751
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PMC:4641751Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A high-throughput standing surface acoustic wave (SSAW)-based cell sorter</title><author><name sortKey="Ren, Liqiang" sort="Ren, Liqiang" uniqKey="Ren L" first="Liqiang" last="Ren">Liqiang Ren</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Chen, Yuchao" sort="Chen, Yuchao" uniqKey="Chen Y" first="Yuchao" last="Chen">Yuchao Chen</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Li, Peng" sort="Li, Peng" uniqKey="Li P" first="Peng" last="Li">Peng Li</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Mao, Zhangming" sort="Mao, Zhangming" uniqKey="Mao Z" first="Zhangming" last="Mao">Zhangming Mao</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Huang, Po Hsun" sort="Huang, Po Hsun" uniqKey="Huang P" first="Po-Hsun" last="Huang">Po-Hsun Huang</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Rufo, Joseph" sort="Rufo, Joseph" uniqKey="Rufo J" first="Joseph" last="Rufo">Joseph Rufo</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Guo, Feng" sort="Guo, Feng" uniqKey="Guo F" first="Feng" last="Guo">Feng Guo</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><affiliation><nlm:aff id="A2">Ascent Bio-Nano Technologies, Inc., State College, PA, 16802, USA</nlm:aff></affiliation></author><author><name sortKey="Mccoy, J Philip" sort="Mccoy, J Philip" uniqKey="Mccoy J" first="J. Philip" last="Mccoy">J. Philip Mccoy</name><affiliation><nlm:aff id="A3">National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD, USA</nlm:aff></affiliation></author><author><name sortKey="Levine, Stewart J" sort="Levine, Stewart J" uniqKey="Levine S" first="Stewart J." last="Levine">Stewart J. Levine</name><affiliation><nlm:aff id="A3">National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD, USA</nlm:aff></affiliation></author><author><name sortKey="Huang, Tony Jun" sort="Huang, Tony Jun" uniqKey="Huang T" first="Tony Jun" last="Huang">Tony Jun Huang</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26289231</idno><idno type="pmc">4641751</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641751</idno><idno type="RBID">PMC:4641751</idno><idno type="doi">10.1039/c5lc00706b</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000428</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A high-throughput standing surface acoustic wave (SSAW)-based cell sorter</title><author><name sortKey="Ren, Liqiang" sort="Ren, Liqiang" uniqKey="Ren L" first="Liqiang" last="Ren">Liqiang Ren</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Chen, Yuchao" sort="Chen, Yuchao" uniqKey="Chen Y" first="Yuchao" last="Chen">Yuchao Chen</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Li, Peng" sort="Li, Peng" uniqKey="Li P" first="Peng" last="Li">Peng Li</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Mao, Zhangming" sort="Mao, Zhangming" uniqKey="Mao Z" first="Zhangming" last="Mao">Zhangming Mao</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Huang, Po Hsun" sort="Huang, Po Hsun" uniqKey="Huang P" first="Po-Hsun" last="Huang">Po-Hsun Huang</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Rufo, Joseph" sort="Rufo, Joseph" uniqKey="Rufo J" first="Joseph" last="Rufo">Joseph Rufo</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Guo, Feng" sort="Guo, Feng" uniqKey="Guo F" first="Feng" last="Guo">Feng Guo</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><affiliation><nlm:aff id="A2">Ascent Bio-Nano Technologies, Inc., State College, PA, 16802, USA</nlm:aff></affiliation></author><author><name sortKey="Mccoy, J Philip" sort="Mccoy, J Philip" uniqKey="Mccoy J" first="J. Philip" last="Mccoy">J. Philip Mccoy</name><affiliation><nlm:aff id="A3">National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD, USA</nlm:aff></affiliation></author><author><name sortKey="Levine, Stewart J" sort="Levine, Stewart J" uniqKey="Levine S" first="Stewart J." last="Levine">Stewart J. Levine</name><affiliation><nlm:aff id="A3">National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD, USA</nlm:aff></affiliation></author><author><name sortKey="Huang, Tony Jun" sort="Huang, Tony Jun" uniqKey="Huang T" first="Tony Jun" last="Huang">Tony Jun Huang</name><affiliation><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff></affiliation></author></analytic><series><title level="j">Lab on a chip</title><idno type="ISSN">1473-0197</idno><idno type="eISSN">1473-0189</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Acoustic-based fluorescence activated cell sorters (FACS) have drawn increased attention in recent years due to their versatility, high biocompatibility, high controllability, and simple design. However, the sorting throughput for existing acoustic cell sorters is far from optimum for practical applications. Here we report a high-throughput cell sorting method based on standing surface acoustic waves (SSAWs). We utilized a pair of focused interdigital transducers (FIDTs) to generate SSAW with high resolution and high energy efficiency. As a result, the sorting throughput is improved significantly from conventional acoustic-based cell sorting methods. We demonstrated the successful sorting of 10 μm polystyrene particles with a minimum actuation time of 72 μs, which translates to a potential sorting rate of more than 13,800 events/s. Without using a cell-detection unit, we were able to demonstrate an actual sorting throughput of 3,300 events/s. Our sorting method can be conveniently integrated with upstream detection units, and it represents an important development towards a functional acoustic-based FACS system.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101128948</journal-id><journal-id journal-id-type="pubmed-jr-id">31848</journal-id><journal-id journal-id-type="nlm-ta">Lab Chip</journal-id><journal-id journal-id-type="iso-abbrev">Lab Chip</journal-id><journal-title-group><journal-title>Lab on a chip</journal-title></journal-title-group><issn pub-type="ppub">1473-0197</issn><issn pub-type="epub">1473-0189</issn></journal-meta><article-meta><article-id pub-id-type="pmid">26289231</article-id><article-id pub-id-type="pmc">4641751</article-id><article-id pub-id-type="doi">10.1039/c5lc00706b</article-id><article-id pub-id-type="manuscript">NIHMS717385</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A high-throughput standing surface acoustic wave (SSAW)-based cell sorter</article-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Ren</surname><given-names>Liqiang</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Chen</surname><given-names>Yuchao</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Peng</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Mao</surname><given-names>Zhangming</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Po-Hsun</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Rufo</surname><given-names>Joseph</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Guo</surname><given-names>Feng</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Lin</given-names></name><xref ref-type="aff" rid="A2">b</xref></contrib><contrib contrib-type="author"><name><surname>McCoy</surname><given-names>J. Philip</given-names></name><xref ref-type="aff" rid="A3">c</xref></contrib><contrib contrib-type="author"><name><surname>Levine</surname><given-names>Stewart J.</given-names></name><xref ref-type="aff" rid="A3">c</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Tony Jun</given-names></name><xref ref-type="corresp" rid="CR1">*</xref><xref ref-type="aff" rid="A1">a</xref></contrib></contrib-group><aff id="A1"><label>a</label>Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</aff><aff id="A2"><label>b</label>Ascent Bio-Nano Technologies, Inc., State College, PA, 16802, USA</aff><aff id="A3"><label>c</label>National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD, USA</aff><author-notes><corresp id="CR1"><label>*</label>To whom correspondence should be addressed: <email>junhuang@psu.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>11</day><month>9</month><year>2015</year></pub-date><pub-date pub-type="ppub"><day>7</day><month>10</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>07</day><month>2</month><year>2016</year></pub-date><volume>15</volume><issue>19</issue><fpage>3870</fpage><lpage>3879</lpage><pmc-comment>elocation-id from pubmed: 10.1039/c5lc00706b</pmc-comment>
<abstract><p id="P1">Acoustic-based fluorescence activated cell sorters (FACS) have drawn increased attention in recent years due to their versatility, high biocompatibility, high controllability, and simple design. However, the sorting throughput for existing acoustic cell sorters is far from optimum for practical applications. Here we report a high-throughput cell sorting method based on standing surface acoustic waves (SSAWs). We utilized a pair of focused interdigital transducers (FIDTs) to generate SSAW with high resolution and high energy efficiency. As a result, the sorting throughput is improved significantly from conventional acoustic-based cell sorting methods. We demonstrated the successful sorting of 10 μm polystyrene particles with a minimum actuation time of 72 μs, which translates to a potential sorting rate of more than 13,800 events/s. Without using a cell-detection unit, we were able to demonstrate an actual sorting throughput of 3,300 events/s. Our sorting method can be conveniently integrated with upstream detection units, and it represents an important development towards a functional acoustic-based FACS system.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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