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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Utilization of Heme as an Iron Source by Marine <named-content content-type="genus-species">Alphaproteobacteria</named-content> in the <named-content content-type="genus-species">Roseobacter</named-content> Clade</title><author><name sortKey="Roe, Kelly L" sort="Roe, Kelly L" uniqKey="Roe K" first="Kelly L." last="Roe">Kelly L. Roe</name></author><author><name sortKey="Hogle, Shane L" sort="Hogle, Shane L" uniqKey="Hogle S" first="Shane L." last="Hogle">Shane L. Hogle</name></author><author><name sortKey="Barbeau, Katherine A" sort="Barbeau, Katherine A" uniqKey="Barbeau K" first="Katherine A." last="Barbeau">Katherine A. Barbeau</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23872569</idno><idno type="pmc">3754158</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3754158</idno><idno type="RBID">PMC:3754158</idno><idno type="doi">10.1128/AEM.01562-13</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000349</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Utilization of Heme as an Iron Source by Marine <named-content content-type="genus-species">Alphaproteobacteria</named-content> in the <named-content content-type="genus-species">Roseobacter</named-content> Clade</title><author><name sortKey="Roe, Kelly L" sort="Roe, Kelly L" uniqKey="Roe K" first="Kelly L." last="Roe">Kelly L. Roe</name></author><author><name sortKey="Hogle, Shane L" sort="Hogle, Shane L" uniqKey="Hogle S" first="Shane L." last="Hogle">Shane L. Hogle</name></author><author><name sortKey="Barbeau, Katherine A" sort="Barbeau, Katherine A" uniqKey="Barbeau K" first="Katherine A." last="Barbeau">Katherine A. Barbeau</name></author></analytic><series><title level="j">Applied and Environmental Microbiology</title><idno type="ISSN">0099-2240</idno><idno type="eISSN">1098-5336</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The bioavailability and utilization of porphyrin-bound iron, specifically heme, by marine microorganisms have rarely been examined. This study used <named-content content-type="genus-species">Ruegeria</named-content> sp. strain TrichCH4B as a model organism to study heme acquisition by a member of the <named-content content-type="genus-species">Roseobacter</named-content> clade. Analogs of known heme transporter proteins were found within the <named-content content-type="genus-species">Ruegeria</named-content> sp. TrichCH4B genome. The identified heme uptake and utilization system appears to be functional, as the heme genes were upregulated under iron stress, the bacterium could grow on ferric-porphyrin complexes as the sole iron source, and internalization of <sup>55</sup> Fe from ferric protoporphyrin IX was observed. The potential ability to utilize heme in the <named-content content-type="genus-species">Roseobacter</named-content> clade appears to be common, as half of the isolates in the RoseoBase database were found to have a complete heme uptake system. A degenerate primer set was designed and successfully used to identify the putative heme oxygenase gene (<italic>hmus</italic>) in the roseobacter heme uptake system from diverse nonenriched marine environments. This study found that members of the <named-content content-type="genus-species">Roseobacter</named-content> clade are capable of utilizing heme as an iron source and that this capability may be present in all types of marine environments. The results of this study add a new perspective to the current picture of iron cycling in marine systems, whereby relatively refractory intracellular pools of heme-bound iron may be taken up quickly and directly reincorporated into living bacteria without previous degradation or the necessity of a siderophore intermediate.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id><journal-id journal-id-type="iso-abbrev">Appl. Environ. Microbiol</journal-id><journal-id journal-id-type="hwp">aem</journal-id><journal-id journal-id-type="pmc">aem</journal-id><journal-id journal-id-type="publisher-id">AEM</journal-id><journal-title-group><journal-title>Applied and Environmental Microbiology</journal-title></journal-title-group><issn pub-type="ppub">0099-2240</issn><issn pub-type="epub">1098-5336</issn><publisher><publisher-name>American Society for Microbiology</publisher-name><publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23872569</article-id><article-id pub-id-type="pmc">3754158</article-id><article-id pub-id-type="publisher-id">01562-13</article-id><article-id pub-id-type="doi">10.1128/AEM.01562-13</article-id><article-categories><subj-group subj-group-type="heading"><subject>Environmental Microbiology</subject></subj-group></article-categories><title-group><article-title>Utilization of Heme as an Iron Source by Marine <named-content content-type="genus-species">Alphaproteobacteria</named-content> in the <named-content content-type="genus-species">Roseobacter</named-content> Clade</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Roe</surname><given-names>Kelly L.</given-names></name><xref ref-type="author-notes" rid="fn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Hogle</surname><given-names>Shane L.</given-names></name></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Barbeau</surname><given-names>Katherine A.</given-names></name></contrib><aff>Geoscience Research Division, Scripps Institution of Oceanography, La Jolla, California, USA</aff></contrib-group><author-notes><corresp id="cor1">Address correspondence to Katherine A. Barbeau, <email>kbarbeau@ucsd.edu</email>.</corresp><fn id="fn1" fn-type="present-address"><label>*</label><p>Present address: Kelly L. Roe, Department of Chemistry and Geochemistry, Colorado School of Mines, Golden, Colorado, USA.</p></fn></author-notes><pub-date pub-type="ppub"><month>9</month><year>2013</year></pub-date><volume>79</volume><issue>18</issue><fpage>5753</fpage><lpage>5762</lpage><history><date date-type="received"><day>13</day><month>5</month><year>2013</year></date><date date-type="accepted"><day>3</day><month>7</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013, American Society for Microbiology. All Rights Reserved.</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>American Society for Microbiology</copyright-holder></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zam01813005753.pdf"></self-uri><abstract><p>The bioavailability and utilization of porphyrin-bound iron, specifically heme, by marine microorganisms have rarely been examined. This study used <named-content content-type="genus-species">Ruegeria</named-content> sp. strain TrichCH4B as a model organism to study heme acquisition by a member of the <named-content content-type="genus-species">Roseobacter</named-content> clade. Analogs of known heme transporter proteins were found within the <named-content content-type="genus-species">Ruegeria</named-content> sp. TrichCH4B genome. The identified heme uptake and utilization system appears to be functional, as the heme genes were upregulated under iron stress, the bacterium could grow on ferric-porphyrin complexes as the sole iron source, and internalization of <sup>55</sup> Fe from ferric protoporphyrin IX was observed. The potential ability to utilize heme in the <named-content content-type="genus-species">Roseobacter</named-content> clade appears to be common, as half of the isolates in the RoseoBase database were found to have a complete heme uptake system. A degenerate primer set was designed and successfully used to identify the putative heme oxygenase gene (<italic>hmus</italic>) in the roseobacter heme uptake system from diverse nonenriched marine environments. This study found that members of the <named-content content-type="genus-species">Roseobacter</named-content> clade are capable of utilizing heme as an iron source and that this capability may be present in all types of marine environments. The results of this study add a new perspective to the current picture of iron cycling in marine systems, whereby relatively refractory intracellular pools of heme-bound iron may be taken up quickly and directly reincorporated into living bacteria without previous degradation or the necessity of a siderophore intermediate.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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