The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environment.
Identifieur interne : 000059 ( Main/Corpus ); précédent : 000058; suivant : 000060The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environment.
Auteurs : Qin Zhou ; Xiaoyu Gu ; Jiachen Dong ; Chao Zhu ; Zhen Cai ; Dongmei He ; Chi Yang ; Ling Xu ; Jiawei ZhengSource :
- Artificial cells, nanomedicine, and biotechnology [ 2169-141X ] ; 2019.
English descriptors
- KwdEn :
- Animals (MeSH), Bone Regeneration (MeSH), Cell Differentiation (MeSH), Cellular Microenvironment (MeSH), Dogs (MeSH), Inflammation (metabolism), Inflammation (pathology), Inflammation (physiopathology), Male (MeSH), Mesenchymal Stem Cell Transplantation (MeSH), Mesenchymal Stem Cells (cytology), Mesenchymal Stem Cells (metabolism), Neovascularization, Physiologic (MeSH), Osteogenesis (MeSH), Signal Transduction (MeSH), Toll-Like Receptor 2 (genetics), Toll-Like Receptor 2 (metabolism), Tooth Socket (diagnostic imaging), Tooth Socket (metabolism), Tooth Socket (pathology), Tooth Socket (physiopathology), X-Ray Microtomography (MeSH).
- MESH :
- chemical , genetics : Toll-Like Receptor 2.
- cytology : Mesenchymal Stem Cells.
- diagnostic imaging : Tooth Socket.
- metabolism : Inflammation, Mesenchymal Stem Cells, Toll-Like Receptor 2, Tooth Socket.
- pathology : Inflammation, Tooth Socket.
- physiopathology : Inflammation, Tooth Socket.
- Animals, Bone Regeneration, Cell Differentiation, Cellular Microenvironment, Dogs, Male, Mesenchymal Stem Cell Transplantation, Neovascularization, Physiologic, Osteogenesis, Signal Transduction, X-Ray Microtomography.
Abstract
The repair of periodontal bone tissue defects in patients with periodontitis is one of the major challenges for dentists. Stem cell-based bone regeneration has been considered as a promising strategy to restore the lost periodontal bone tissue. However, the local inflammatory environment of periodontal tissue affects stem cell-based periodontal bone regeneration. Toll-like receptor 2 (TLR2), a member of the TLR family, plays an important role in regulating immunoreaction. Previous studies have shown that the activation of TLR2 signaling pathway is involved in enhancing tissue vascularization and wound healing. However, the mechanisms underlying the therapeutic effects of TLR2 on regulating bone marrow stromal cells (BMSCs) mediated periodontal bone tissue regeneration still need to be further investigated. In this study, we tested the effect of TLR2 on regulating BMSCs mediated alveolar bone regeneration by establishing a TLR2 gene-modified canine BMSCs using a lentivirus. Activation of TLR2 significantly enhanced the expression of hypoxia-inducible factor-1α (HIF-1α) and bone morphogenetic protein 2 (BMP-2) and then upregulated the expression of their downstream osteogenic and angiogenic related gene in BMSCs. TLR2-BMSCs mediated bone regeneration in canine tooth extraction sockets under an inflammatory environment demonstrated that activation of the TLR2 signaling pathway significantly stimulated BMSCs meditated angiogenesis and osteogenesis.
DOI: 10.1080/21691401.2019.1626867
PubMed: 31387403
Links to Exploration step
pubmed:31387403Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environment.</title><author><name sortKey="Zhou, Qin" sort="Zhou, Qin" uniqKey="Zhou Q" first="Qin" last="Zhou">Qin Zhou</name><affiliation><nlm:affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Gu, Xiaoyu" sort="Gu, Xiaoyu" uniqKey="Gu X" first="Xiaoyu" last="Gu">Xiaoyu Gu</name><affiliation><nlm:affiliation>b Department of Prothodontics, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Dong, Jiachen" sort="Dong, Jiachen" uniqKey="Dong J" first="Jiachen" last="Dong">Jiachen Dong</name><affiliation><nlm:affiliation>c Department of Periodontology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Chao" sort="Zhu, Chao" uniqKey="Zhu C" first="Chao" last="Zhu">Chao Zhu</name><affiliation><nlm:affiliation>d Department of Oral and Maxillofacial Surgery, Shandong Linyi People's Hospital , Linyi , China.</nlm:affiliation></affiliation></author><author><name sortKey="Cai, Zhen" sort="Cai, Zhen" uniqKey="Cai Z" first="Zhen" last="Cai">Zhen Cai</name><affiliation><nlm:affiliation>d Department of Oral and Maxillofacial Surgery, Shandong Linyi People's Hospital , Linyi , China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Dongmei" sort="He, Dongmei" uniqKey="He D" first="Dongmei" last="He">Dongmei He</name><affiliation><nlm:affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Chi" sort="Yang, Chi" uniqKey="Yang C" first="Chi" last="Yang">Chi Yang</name><affiliation><nlm:affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Ling" sort="Xu, Ling" uniqKey="Xu L" first="Ling" last="Xu">Ling Xu</name><affiliation><nlm:affiliation>b Department of Prothodontics, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>e Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Zheng, Jiawei" sort="Zheng, Jiawei" uniqKey="Zheng J" first="Jiawei" last="Zheng">Jiawei Zheng</name><affiliation><nlm:affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31387403</idno><idno type="pmid">31387403</idno><idno type="doi">10.1080/21691401.2019.1626867</idno><idno type="wicri:Area/Main/Corpus">000059</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000059</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environment.</title><author><name sortKey="Zhou, Qin" sort="Zhou, Qin" uniqKey="Zhou Q" first="Qin" last="Zhou">Qin Zhou</name><affiliation><nlm:affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Gu, Xiaoyu" sort="Gu, Xiaoyu" uniqKey="Gu X" first="Xiaoyu" last="Gu">Xiaoyu Gu</name><affiliation><nlm:affiliation>b Department of Prothodontics, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Dong, Jiachen" sort="Dong, Jiachen" uniqKey="Dong J" first="Jiachen" last="Dong">Jiachen Dong</name><affiliation><nlm:affiliation>c Department of Periodontology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Chao" sort="Zhu, Chao" uniqKey="Zhu C" first="Chao" last="Zhu">Chao Zhu</name><affiliation><nlm:affiliation>d Department of Oral and Maxillofacial Surgery, Shandong Linyi People's Hospital , Linyi , China.</nlm:affiliation></affiliation></author><author><name sortKey="Cai, Zhen" sort="Cai, Zhen" uniqKey="Cai Z" first="Zhen" last="Cai">Zhen Cai</name><affiliation><nlm:affiliation>d Department of Oral and Maxillofacial Surgery, Shandong Linyi People's Hospital , Linyi , China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Dongmei" sort="He, Dongmei" uniqKey="He D" first="Dongmei" last="He">Dongmei He</name><affiliation><nlm:affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Chi" sort="Yang, Chi" uniqKey="Yang C" first="Chi" last="Yang">Chi Yang</name><affiliation><nlm:affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Ling" sort="Xu, Ling" uniqKey="Xu L" first="Ling" last="Xu">Ling Xu</name><affiliation><nlm:affiliation>b Department of Prothodontics, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>e Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology , Shanghai , China.</nlm:affiliation></affiliation></author><author><name sortKey="Zheng, Jiawei" sort="Zheng, Jiawei" uniqKey="Zheng J" first="Jiawei" last="Zheng">Jiawei Zheng</name><affiliation><nlm:affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Artificial cells, nanomedicine, and biotechnology</title><idno type="eISSN">2169-141X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Bone Regeneration (MeSH)</term><term>Cell Differentiation (MeSH)</term><term>Cellular Microenvironment (MeSH)</term><term>Dogs (MeSH)</term><term>Inflammation (metabolism)</term><term>Inflammation (pathology)</term><term>Inflammation (physiopathology)</term><term>Male (MeSH)</term><term>Mesenchymal Stem Cell Transplantation (MeSH)</term><term>Mesenchymal Stem Cells (cytology)</term><term>Mesenchymal Stem Cells (metabolism)</term><term>Neovascularization, Physiologic (MeSH)</term><term>Osteogenesis (MeSH)</term><term>Signal Transduction (MeSH)</term><term>Toll-Like Receptor 2 (genetics)</term><term>Toll-Like Receptor 2 (metabolism)</term><term>Tooth Socket (diagnostic imaging)</term><term>Tooth Socket (metabolism)</term><term>Tooth Socket (pathology)</term><term>Tooth Socket (physiopathology)</term><term>X-Ray Microtomography (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Toll-Like Receptor 2</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Mesenchymal Stem Cells</term></keywords><keywords scheme="MESH" qualifier="diagnostic imaging" xml:lang="en"><term>Tooth Socket</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Inflammation</term><term>Mesenchymal Stem Cells</term><term>Toll-Like Receptor 2</term><term>Tooth Socket</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Inflammation</term><term>Tooth Socket</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Inflammation</term><term>Tooth Socket</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Bone Regeneration</term><term>Cell Differentiation</term><term>Cellular Microenvironment</term><term>Dogs</term><term>Male</term><term>Mesenchymal Stem Cell Transplantation</term><term>Neovascularization, Physiologic</term><term>Osteogenesis</term><term>Signal Transduction</term><term>X-Ray Microtomography</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The repair of periodontal bone tissue defects in patients with periodontitis is one of the major challenges for dentists. Stem cell-based bone regeneration has been considered as a promising strategy to restore the lost periodontal bone tissue. However, the local inflammatory environment of periodontal tissue affects stem cell-based periodontal bone regeneration. Toll-like receptor 2 (TLR2), a member of the TLR family, plays an important role in regulating immunoreaction. Previous studies have shown that the activation of TLR2 signaling pathway is involved in enhancing tissue vascularization and wound healing. However, the mechanisms underlying the therapeutic effects of TLR2 on regulating bone marrow stromal cells (BMSCs) mediated periodontal bone tissue regeneration still need to be further investigated. In this study, we tested the effect of TLR2 on regulating BMSCs mediated alveolar bone regeneration by establishing a TLR2 gene-modified canine BMSCs using a lentivirus. Activation of TLR2 significantly enhanced the expression of hypoxia-inducible factor-1α (HIF-1α) and bone morphogenetic protein 2 (BMP-2) and then upregulated the expression of their downstream osteogenic and angiogenic related gene in BMSCs. TLR2-BMSCs mediated bone regeneration in canine tooth extraction sockets under an inflammatory environment demonstrated that activation of the TLR2 signaling pathway significantly stimulated BMSCs meditated angiogenesis and osteogenesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31387403</PMID><DateCompleted><Year>2019</Year><Month>12</Month><Day>30</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2169-141X</ISSN><JournalIssue CitedMedium="Internet"><Volume>47</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Artificial cells, nanomedicine, and biotechnology</Title><ISOAbbreviation>Artif Cells Nanomed Biotechnol</ISOAbbreviation></Journal><ArticleTitle>The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environment.</ArticleTitle><Pagination><MedlinePgn>3329-3337</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/21691401.2019.1626867</ELocationID><Abstract><AbstractText>The repair of periodontal bone tissue defects in patients with periodontitis is one of the major challenges for dentists. Stem cell-based bone regeneration has been considered as a promising strategy to restore the lost periodontal bone tissue. However, the local inflammatory environment of periodontal tissue affects stem cell-based periodontal bone regeneration. Toll-like receptor 2 (TLR2), a member of the TLR family, plays an important role in regulating immunoreaction. Previous studies have shown that the activation of TLR2 signaling pathway is involved in enhancing tissue vascularization and wound healing. However, the mechanisms underlying the therapeutic effects of TLR2 on regulating bone marrow stromal cells (BMSCs) mediated periodontal bone tissue regeneration still need to be further investigated. In this study, we tested the effect of TLR2 on regulating BMSCs mediated alveolar bone regeneration by establishing a TLR2 gene-modified canine BMSCs using a lentivirus. Activation of TLR2 significantly enhanced the expression of hypoxia-inducible factor-1α (HIF-1α) and bone morphogenetic protein 2 (BMP-2) and then upregulated the expression of their downstream osteogenic and angiogenic related gene in BMSCs. TLR2-BMSCs mediated bone regeneration in canine tooth extraction sockets under an inflammatory environment demonstrated that activation of the TLR2 signaling pathway significantly stimulated BMSCs meditated angiogenesis and osteogenesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Qin</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gu</LastName><ForeName>Xiaoyu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>b Department of Prothodontics, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Jiachen</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-6476-9180</Identifier><AffiliationInfo><Affiliation>c Department of Periodontology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Chao</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>d Department of Oral and Maxillofacial Surgery, Shandong Linyi People's Hospital , Linyi , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>Zhen</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>d Department of Oral and Maxillofacial Surgery, Shandong Linyi People's Hospital , Linyi , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Dongmei</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chi</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Ling</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>b Department of Prothodontics, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>e Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology , Shanghai , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Jiawei</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>a Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai , China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Artif Cells Nanomed Biotechnol</MedlineTA><NlmUniqueID>101594777</NlmUniqueID><ISSNLinking>2169-1401</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051195">Toll-Like Receptor 2</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001861" MajorTopicYN="Y">Bone Regeneration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060833" MajorTopicYN="Y">Cellular Microenvironment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004285" MajorTopicYN="N">Dogs</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007249" MajorTopicYN="N">Inflammation</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000503" MajorTopicYN="N">physiopathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045164" MajorTopicYN="N">Mesenchymal Stem Cell Transplantation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059630" MajorTopicYN="N">Mesenchymal Stem Cells</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018919" MajorTopicYN="N">Neovascularization, Physiologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010012" MajorTopicYN="N">Osteogenesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051195" MajorTopicYN="N">Toll-Like Receptor 2</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020390" MajorTopicYN="N">Tooth Socket</DescriptorName><QualifierName UI="Q000000981" MajorTopicYN="N">diagnostic imaging</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000503" MajorTopicYN="N">physiopathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055114" MajorTopicYN="N">X-Ray Microtomography</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">BMSCs</Keyword><Keyword MajorTopicYN="N">TLR2</Keyword><Keyword MajorTopicYN="N">inflammatory</Keyword><Keyword MajorTopicYN="N">micro-environment</Keyword><Keyword MajorTopicYN="N">periodontal bone regeneration</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31387403</ArticleId><ArticleId IdType="doi">10.1080/21691401.2019.1626867</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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