StressCovidV1, PubMed, Curation, bibRecord, 000273

Multiple nuclear-replicating viruses require the stress-induced protein ZC3H11A for efficient growth.

Identifieur interne : 000273 ( PubMed/Curation ); précédent : 000272; suivant : 000274

Multiple nuclear-replicating viruses require the stress-induced protein ZC3H11A for efficient growth.

Auteurs : Shady Younis [Suède] ; Wael Kamel [Suède] ; Tina Falkeborn [Suède] ; Hao Wang [Suède] ; Di Yu [Suède] ; Robert Daniels [Suède] ; Magnus Essand [Suède] ; Jorma Hinkula [Suède] ; Göran Akusj Rvi [Suède] ; Leif Andersson [Suède]

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2018.

RBID : pubmed:29610341

Descripteurs français

English descriptors

KwdEn :
- Adenoviruses, Human (genetics), Adenoviruses, Human (physiology), Binding Sites, Biological Transport, CRISPR-Cas Systems, Cell Nucleus (metabolism), Cell Nucleus (virology), Cytoplasm (virology), Gene Knockout Techniques, HeLa Cells, Heat-Shock Response (genetics), Heat-Shock Response (physiology), High-Throughput Nucleotide Sequencing, Host-Pathogen Interactions, Humans, Nuclear Proteins (antagonists & inhibitors), Nuclear Proteins (physiology), Protein Domains, Protein Stability, RNA, Messenger (metabolism), RNA, Viral (metabolism), RNA-Binding Proteins (antagonists & inhibitors), RNA-Binding Proteins (physiology), Serine-Arginine Splicing Factors (physiology), Virus Replication, Zinc Fingers (physiology).
MESH :
- chemical , antagonists & inhibitors : Nuclear Proteins, RNA-Binding Proteins.
- genetics : Adenoviruses, Human, Heat-Shock Response.
- metabolism : Cell Nucleus, RNA, Messenger, RNA, Viral.
- physiology : Adenoviruses, Human, Heat-Shock Response, Nuclear Proteins, RNA-Binding Proteins, Serine-Arginine Splicing Factors, Zinc Fingers.
- virology : Cell Nucleus, Cytoplasm.
- Binding Sites, Biological Transport, CRISPR-Cas Systems, Gene Knockout Techniques, HeLa Cells, High-Throughput Nucleotide Sequencing, Host-Pathogen Interactions, Humans, Protein Domains, Protein Stability, Virus Replication.

Abstract

The zinc finger CCCH-type containing 11A (ZC3H11A) gene encodes a well-conserved zinc finger protein that may function in mRNA export as it has been shown to associate with the transcription export (TREX) complex in proteomic screens. Here, we report that ZC3H11A is a stress-induced nuclear protein with RNA-binding capacity that localizes to nuclear splicing speckles. During an adenovirus infection, the ZC3H11A protein and splicing factor SRSF2 relocalize to nuclear regions where viral DNA replication and transcription take place. Knockout (KO) of ZC3H11A in HeLa cells demonstrated that several nuclear-replicating viruses are dependent on ZC3H11A for efficient growth (HIV, influenza virus, herpes simplex virus, and adenovirus), whereas cytoplasmic replicating viruses are not (vaccinia virus and Semliki Forest virus). High-throughput sequencing of ZC3H11A-cross-linked RNA showed that ZC3H11A binds to short purine-rich ribonucleotide stretches in cellular and adenoviral transcripts. We show that the RNA-binding property of ZC3H11A is crucial for its function and localization. In ZC3H11A KO cells, the adenovirus fiber mRNA accumulates in the cell nucleus. Our results suggest that ZC3H11A is important for maintaining nuclear export of mRNAs during stress and that several nuclear-replicating viruses take advantage of this mechanism to facilitate their replication.

DOI: 10.1073/pnas.1722333115
PubMed: 29610341

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<term>Adenoviruses, Human (physiology)</term>
<term>Binding Sites</term>
<term>Biological Transport</term>
<term>CRISPR-Cas Systems</term>
<term>Cell Nucleus (metabolism)</term>
<term>Cell Nucleus (virology)</term>
<term>Cytoplasm (virology)</term>
<term>Gene Knockout Techniques</term>
<term>HeLa Cells</term>
<term>Heat-Shock Response (genetics)</term>
<term>Heat-Shock Response (physiology)</term>
<term>High-Throughput Nucleotide Sequencing</term>
<term>Host-Pathogen Interactions</term>
<term>Humans</term>
<term>Nuclear Proteins (antagonists & inhibitors)</term>
<term>Nuclear Proteins (physiology)</term>
<term>Protein Domains</term>
<term>Protein Stability</term>
<term>RNA, Messenger (metabolism)</term>
<term>RNA, Viral (metabolism)</term>
<term>RNA-Binding Proteins (antagonists & inhibitors)</term>
<term>RNA-Binding Proteins (physiology)</term>
<term>Serine-Arginine Splicing Factors (physiology)</term>
<term>Virus Replication</term>
<term>Zinc Fingers (physiology)</term>
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<term>ARN viral (métabolisme)</term>
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<term>Adénovirus humains (physiologie)</term>
<term>Cellules HeLa</term>
<term>Cytoplasme (virologie)</term>
<term>Doigts de zinc (physiologie)</term>
<term>Domaines protéiques</term>
<term>Facteurs d'épissage riches en sérine-arginine (physiologie)</term>
<term>Humains</term>
<term>Interactions hôte-pathogène</term>
<term>Noyau de la cellule (métabolisme)</term>
<term>Noyau de la cellule (virologie)</term>
<term>Protéines de liaison à l'ARN (antagonistes et inhibiteurs)</term>
<term>Protéines de liaison à l'ARN (physiologie)</term>
<term>Protéines nucléaires (antagonistes et inhibiteurs)</term>
<term>Protéines nucléaires (physiologie)</term>
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<term>Techniques de knock-out de gènes</term>
<term>Transport biologique</term>
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<term>RNA-Binding Proteins</term>
<term>Serine-Arginine Splicing Factors</term>
<term>Zinc Fingers</term>
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<term>Noyau de la cellule</term>
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<term>Cytoplasm</term>
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<term>Biological Transport</term>
<term>CRISPR-Cas Systems</term>
<term>Gene Knockout Techniques</term>
<term>HeLa Cells</term>
<term>High-Throughput Nucleotide Sequencing</term>
<term>Host-Pathogen Interactions</term>
<term>Humans</term>
<term>Protein Domains</term>
<term>Protein Stability</term>
<term>Virus Replication</term>
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<term>Domaines protéiques</term>
<term>Humains</term>
<term>Interactions hôte-pathogène</term>
<term>Réplication virale</term>
<term>Sites de fixation</term>
<term>Stabilité protéique</term>
<term>Systèmes CRISPR-Cas</term>
<term>Séquençage nucléotidique à haut débit</term>
<term>Techniques de knock-out de gènes</term>
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<front><div type="abstract" xml:lang="en">The zinc finger CCCH-type containing 11A (<i>ZC3H11A</i>
) gene encodes a well-conserved zinc finger protein that may function in mRNA export as it has been shown to associate with the transcription export (TREX) complex in proteomic screens. Here, we report that ZC3H11A is a stress-induced nuclear protein with RNA-binding capacity that localizes to nuclear splicing speckles. During an adenovirus infection, the ZC3H11A protein and splicing factor SRSF2 relocalize to nuclear regions where viral DNA replication and transcription take place. Knockout (KO) of <i>ZC3H11A</i>
 in HeLa cells demonstrated that several nuclear-replicating viruses are dependent on ZC3H11A for efficient growth (HIV, influenza virus, herpes simplex virus, and adenovirus), whereas cytoplasmic replicating viruses are not (vaccinia virus and Semliki Forest virus). High-throughput sequencing of ZC3H11A-cross-linked RNA showed that ZC3H11A binds to short purine-rich ribonucleotide stretches in cellular and adenoviral transcripts. We show that the RNA-binding property of ZC3H11A is crucial for its function and localization. In ZC3H11A KO cells, the adenovirus fiber mRNA accumulates in the cell nucleus. Our results suggest that ZC3H11A is important for maintaining nuclear export of mRNAs during stress and that several nuclear-replicating viruses take advantage of this mechanism to facilitate their replication.</div>
</front>
</TEI>
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<DateCompleted><Year>2018</Year>
<Month>08</Month>
<Day>29</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>14</Day>
</DateRevised>
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<Issue>16</Issue>
<PubDate><Year>2018</Year>
<Month>04</Month>
<Day>17</Day>
</PubDate>
</JournalIssue>
<Title>Proceedings of the National Academy of Sciences of the United States of America</Title>
<ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation>
</Journal>
<ArticleTitle>Multiple nuclear-replicating viruses require the stress-induced protein ZC3H11A for efficient growth.</ArticleTitle>
<Pagination><MedlinePgn>E3808-E3816</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.1722333115</ELocationID>
<Abstract><AbstractText>The zinc finger CCCH-type containing 11A (<i>ZC3H11A</i>
) gene encodes a well-conserved zinc finger protein that may function in mRNA export as it has been shown to associate with the transcription export (TREX) complex in proteomic screens. Here, we report that ZC3H11A is a stress-induced nuclear protein with RNA-binding capacity that localizes to nuclear splicing speckles. During an adenovirus infection, the ZC3H11A protein and splicing factor SRSF2 relocalize to nuclear regions where viral DNA replication and transcription take place. Knockout (KO) of <i>ZC3H11A</i>
 in HeLa cells demonstrated that several nuclear-replicating viruses are dependent on ZC3H11A for efficient growth (HIV, influenza virus, herpes simplex virus, and adenovirus), whereas cytoplasmic replicating viruses are not (vaccinia virus and Semliki Forest virus). High-throughput sequencing of ZC3H11A-cross-linked RNA showed that ZC3H11A binds to short purine-rich ribonucleotide stretches in cellular and adenoviral transcripts. We show that the RNA-binding property of ZC3H11A is crucial for its function and localization. In ZC3H11A KO cells, the adenovirus fiber mRNA accumulates in the cell nucleus. Our results suggest that ZC3H11A is important for maintaining nuclear export of mRNAs during stress and that several nuclear-replicating viruses take advantage of this mechanism to facilitate their replication.</AbstractText>
<CopyrightInformation>Copyright © 2018 the Author(s). Published by PNAS.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Younis</LastName>
<ForeName>Shady</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Medical Biochemistry and Microbiology, Uppsala University, SE-751 23 Uppsala, Sweden.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Animal Production, Ain Shams University, Shoubra El-Kheima, 11241 Cairo, Egypt.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kamel</LastName>
<ForeName>Wael</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Department of Medical Biochemistry and Microbiology, Uppsala University, SE-751 23 Uppsala, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Falkeborn</LastName>
<ForeName>Tina</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Clinical and Experimental Medicine, Linköping University, SE-58183 Linköping, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Hao</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry and Biophysics, Stockholm University, SE-10691 Stockholm, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
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<Initials>J</Initials>
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</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, SE-75007 Uppsala, Sweden.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77483.</Affiliation>
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</MeshHeading>
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<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
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<QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
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<MeshHeading><DescriptorName UI="D000072417" MajorTopicYN="N">Protein Domains</DescriptorName>
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<MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName>
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<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
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<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014779" MajorTopicYN="Y">Virus Replication</DescriptorName>
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<MeshHeading><DescriptorName UI="D016335" MajorTopicYN="N">Zinc Fingers</DescriptorName>
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<Keyword MajorTopicYN="Y">mRNA export</Keyword>
<Keyword MajorTopicYN="Y">stress response</Keyword>
<Keyword MajorTopicYN="Y">virus infection</Keyword>
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<CoiStatement>Conflict of interest statement: S.Y., W.K., G.A., and L.A. are coauthors on a patent application filed based on some of the results in this study.</CoiStatement>
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Serveur d'exploration Stress et Covid

Multiple nuclear-replicating viruses require the stress-induced protein ZC3H11A for efficient growth.

Multiple nuclear-replicating viruses require the stress-induced protein ZC3H11A for efficient growth.

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