Ezrin and HNRNP expression correlate with increased virus release rate and early onset of virus-induced apoptosis of MDCK suspension cells.
Identifieur interne : 000347 ( PubMed/Corpus ); précédent : 000346; suivant : 000348Ezrin and HNRNP expression correlate with increased virus release rate and early onset of virus-induced apoptosis of MDCK suspension cells.
Auteurs : Sabine Kluge ; Yvonne Genzel ; Kim Laus ; Anja Serve ; Antje Pflugmacher ; Britta Peschel ; Erdmann Rapp ; Udo ReichlSource :
- Biotechnology journal [ 1860-7314 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Cytoskeletal Proteins, Heterogeneous-Nuclear Ribonucleoproteins.
- methods : Virus Cultivation.
- physiology : Influenza A Virus, H1N1 Subtype.
- Animals, Apoptosis, Cell Culture Techniques, Dogs, Madin Darby Canine Kidney Cells, Proteomics, Virus Release.
Abstract
With the aim to adapt high-yield adherent cell lines to suspension growth, Madin Darby canine kidney (MDCK) suspension cells were developed recently that achieved comparable influenza virus yields despite an early induction of apoptosis compared to the parental adherent cell line. For both cell lines, a comprehensive study under comparable infection conditions is performed comprising information on: time course of viral infection, antiviral state of cells, virus-induced apoptosis, and virus-induced cellular protein expression for early and late infection with influenza A/PuertoRico/8/34 H1N1. The proteomic analysis is performed with 2D differential gel electrophoreses followed by mass spectrometry. Based on flow cytometric data and on the differential expression of various stress and apoptosis-related proteins, the earlier onset of virus-induced apoptosis is confirmed for suspension cells. Surprisingly, the data indicated an increased virus release rate for suspension cells. These observations correlate with an increased expression of the apical marker protein ezrin, known to play a role in influenza-induced cytoskeletal rearrangement, and the differential expression of heterogeneous nuclear ribonucleoproteins, known to bind actively influenza viral proteins and play a central role in regulating gene expression. Based on these findings, additional studies towards the design of MDCK suspension cells with further increase in influenza virus yields will be performed.
DOI: 10.1002/biot.201600384
PubMed: 27581796
Links to Exploration step
pubmed:27581796Le document en format XML
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for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Serve, Anja" sort="Serve, Anja" uniqKey="Serve A" first="Anja" last="Serve">Anja Serve</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Pflugmacher, Antje" sort="Pflugmacher, Antje" uniqKey="Pflugmacher A" first="Antje" last="Pflugmacher">Antje Pflugmacher</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Peschel, Britta" sort="Peschel, Britta" uniqKey="Peschel B" first="Britta" last="Peschel">Britta Peschel</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Rapp, Erdmann" sort="Rapp, Erdmann" uniqKey="Rapp E" first="Erdmann" last="Rapp">Erdmann Rapp</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Reichl, Udo" sort="Reichl, Udo" uniqKey="Reichl U" first="Udo" last="Reichl">Udo Reichl</name><affiliation><nlm:affiliation>Chair of Bioprocess Engineering, Otto von Guericke University, Magdeburg, Germany.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27581796</idno><idno type="pmid">27581796</idno><idno type="doi">10.1002/biot.201600384</idno><idno type="wicri:Area/PubMed/Corpus">000347</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000347</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ezrin and HNRNP expression correlate with increased virus release rate and early onset of virus-induced apoptosis of MDCK suspension cells.</title><author><name sortKey="Kluge, Sabine" sort="Kluge, Sabine" uniqKey="Kluge S" first="Sabine" last="Kluge">Sabine Kluge</name><affiliation><nlm:affiliation>Chair of Bioprocess Engineering, Otto von Guericke University, Magdeburg, Germany. sabine.pech@roche.de.</nlm:affiliation></affiliation></author><author><name sortKey="Genzel, Yvonne" sort="Genzel, Yvonne" uniqKey="Genzel Y" first="Yvonne" last="Genzel">Yvonne Genzel</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Laus, Kim" sort="Laus, Kim" uniqKey="Laus K" first="Kim" last="Laus">Kim Laus</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Serve, Anja" sort="Serve, Anja" uniqKey="Serve A" first="Anja" last="Serve">Anja Serve</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Pflugmacher, Antje" sort="Pflugmacher, Antje" uniqKey="Pflugmacher A" first="Antje" last="Pflugmacher">Antje Pflugmacher</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Peschel, Britta" sort="Peschel, Britta" uniqKey="Peschel B" first="Britta" last="Peschel">Britta Peschel</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Rapp, Erdmann" sort="Rapp, Erdmann" uniqKey="Rapp E" first="Erdmann" last="Rapp">Erdmann Rapp</name><affiliation><nlm:affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Reichl, Udo" sort="Reichl, Udo" uniqKey="Reichl U" first="Udo" last="Reichl">Udo Reichl</name><affiliation><nlm:affiliation>Chair of Bioprocess Engineering, Otto von Guericke University, Magdeburg, Germany.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Biotechnology journal</title><idno type="eISSN">1860-7314</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Cell Culture Techniques</term><term>Cytoskeletal Proteins (metabolism)</term><term>Dogs</term><term>Heterogeneous-Nuclear Ribonucleoproteins (metabolism)</term><term>Influenza A Virus, H1N1 Subtype (physiology)</term><term>Madin Darby Canine Kidney Cells</term><term>Proteomics</term><term>Virus Cultivation (methods)</term><term>Virus Release</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytoskeletal Proteins</term><term>Heterogeneous-Nuclear Ribonucleoproteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Virus Cultivation</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Cell Culture Techniques</term><term>Dogs</term><term>Madin Darby Canine Kidney Cells</term><term>Proteomics</term><term>Virus Release</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">With the aim to adapt high-yield adherent cell lines to suspension growth, Madin Darby canine kidney (MDCK) suspension cells were developed recently that achieved comparable influenza virus yields despite an early induction of apoptosis compared to the parental adherent cell line. For both cell lines, a comprehensive study under comparable infection conditions is performed comprising information on: time course of viral infection, antiviral state of cells, virus-induced apoptosis, and virus-induced cellular protein expression for early and late infection with influenza A/PuertoRico/8/34 H1N1. The proteomic analysis is performed with 2D differential gel electrophoreses followed by mass spectrometry. Based on flow cytometric data and on the differential expression of various stress and apoptosis-related proteins, the earlier onset of virus-induced apoptosis is confirmed for suspension cells. Surprisingly, the data indicated an increased virus release rate for suspension cells. These observations correlate with an increased expression of the apical marker protein ezrin, known to play a role in influenza-induced cytoskeletal rearrangement, and the differential expression of heterogeneous nuclear ribonucleoproteins, known to bind actively influenza viral proteins and play a central role in regulating gene expression. Based on these findings, additional studies towards the design of MDCK suspension cells with further increase in influenza virus yields will be performed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27581796</PMID><DateCompleted><Year>2017</Year><Month>01</Month><Day>19</Day></DateCompleted><DateRevised><Year>2017</Year><Month>01</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1860-7314</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>10</Issue><PubDate><Year>2016</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Biotechnology journal</Title><ISOAbbreviation>Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Ezrin and HNRNP expression correlate with increased virus release rate and early onset of virus-induced apoptosis of MDCK suspension cells.</ArticleTitle><Pagination><MedlinePgn>1332-1342</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/biot.201600384</ELocationID><Abstract><AbstractText>With the aim to adapt high-yield adherent cell lines to suspension growth, Madin Darby canine kidney (MDCK) suspension cells were developed recently that achieved comparable influenza virus yields despite an early induction of apoptosis compared to the parental adherent cell line. For both cell lines, a comprehensive study under comparable infection conditions is performed comprising information on: time course of viral infection, antiviral state of cells, virus-induced apoptosis, and virus-induced cellular protein expression for early and late infection with influenza A/PuertoRico/8/34 H1N1. The proteomic analysis is performed with 2D differential gel electrophoreses followed by mass spectrometry. Based on flow cytometric data and on the differential expression of various stress and apoptosis-related proteins, the earlier onset of virus-induced apoptosis is confirmed for suspension cells. Surprisingly, the data indicated an increased virus release rate for suspension cells. These observations correlate with an increased expression of the apical marker protein ezrin, known to play a role in influenza-induced cytoskeletal rearrangement, and the differential expression of heterogeneous nuclear ribonucleoproteins, known to bind actively influenza viral proteins and play a central role in regulating gene expression. Based on these findings, additional studies towards the design of MDCK suspension cells with further increase in influenza virus yields will be performed.</AbstractText><CopyrightInformation>Copyright © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kluge</LastName><ForeName>Sabine</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Chair of Bioprocess Engineering, Otto von Guericke University, Magdeburg, Germany. sabine.pech@roche.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Genzel</LastName><ForeName>Yvonne</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laus</LastName><ForeName>Kim</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Serve</LastName><ForeName>Anja</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pflugmacher</LastName><ForeName>Antje</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peschel</LastName><ForeName>Britta</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rapp</LastName><ForeName>Erdmann</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reichl</LastName><ForeName>Udo</ForeName><Initials>U</Initials><AffiliationInfo><Affiliation>Chair of Bioprocess Engineering, Otto von Guericke University, Magdeburg, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Biotechnol J</MedlineTA><NlmUniqueID>101265833</NlmUniqueID><ISSNLinking>1860-6768</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003598">Cytoskeletal 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MajorTopicYN="N">Heterogeneous-Nuclear Ribonucleoproteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D061985" MajorTopicYN="N">Madin Darby Canine Kidney Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014776" MajorTopicYN="N">Virus Cultivation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057074" MajorTopicYN="N">Virus Release</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cell Culture</Keyword><Keyword MajorTopicYN="N">Omics</Keyword><Keyword 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