The NS1 protein of avian influenza virus H9N2 induces oxidative-stress-mediated chicken oviduct epithelial cells apoptosis.
Identifieur interne : 000334 ( PubMed/Corpus ); précédent : 000333; suivant : 000335The NS1 protein of avian influenza virus H9N2 induces oxidative-stress-mediated chicken oviduct epithelial cells apoptosis.
Auteurs : Xuefeng Qi ; Huizhu Zhang ; Qiuzhen Wang ; Jingyu WangSource :
- The Journal of general virology [ 1465-2099 ] ; 2016.
English descriptors
- KwdEn :
- Animals, Apoptosis, Caspases (metabolism), Chickens, Epithelial Cells (cytology), Epithelial Cells (metabolism), Epithelial Cells (virology), Female, Influenza A Virus, H9N2 Subtype (genetics), Influenza A Virus, H9N2 Subtype (metabolism), Influenza in Birds (metabolism), Influenza in Birds (physiopathology), Influenza in Birds (virology), Mitochondria (metabolism), Oviducts (cytology), Oviducts (metabolism), Oviducts (virology), Oxidative Stress, Poultry Diseases (metabolism), Poultry Diseases (physiopathology), Poultry Diseases (virology), Reactive Oxygen Species (metabolism), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism).
- MESH :
- chemical , genetics : Viral Nonstructural Proteins.
- chemical , metabolism : Caspases, Reactive Oxygen Species, Viral Nonstructural Proteins.
- cytology : Epithelial Cells, Oviducts.
- genetics : Influenza A Virus, H9N2 Subtype.
- metabolism : Epithelial Cells, Influenza A Virus, H9N2 Subtype, Influenza in Birds, Mitochondria, Oviducts, Poultry Diseases.
- physiopathology : Influenza in Birds, Poultry Diseases.
- virology : Epithelial Cells, Influenza in Birds, Oviducts, Poultry Diseases.
- Animals, Apoptosis, Chickens, Female, Oxidative Stress.
Abstract
The pathogenesis of H9N2 subtype avian influenza virus infection (AIV) in hens is often related to oviduct tissue damage. The viral non-structural NS1 protein is thought to play a key role in regulating the pathogenicity of AIV, but its exact function in this process remains elusive. In this study, the pro-apoptosis effect of H9N2 NS1 protein was examined on chicken oviduct epithelial cells (COECs) and our data indicated that NS1-induced oxidative stress was a contributing factor in apoptosis. Our data indicate that NS1 protein level was correlated with reactive oxygen species (ROS) in COECs transfected with NS1 expression plasmids. Interestingly, decreased activities of antioxidant enzymes, superoxide dismutase and catalase, were observed in NS1-transfected COECs. Treatment of COECs with antioxidants, such as pyrrolidine dithiocarbamate (PDTC) or N-acetylcysteine (NAC), significantly inhibited NS1-induced apoptosis. Moreover, although antioxidant treatment has little effect on the activation of caspase-8 in NS1-transfected cells, the activation of caspase-3/9 and Bax/Bcl-2 were significantly downregulated. Taken together, the results of our study demonstrated that expression of H9N2 NS1 alone is sufficient to trigger oxidative stress in COECs. Additionally, NS1 protein can induce cellular apoptosis via activating ROS accumulation and mitochondria-mediated apoptotic signalling in COECs.
DOI: 10.1099/jgv.0.000625
PubMed: 27902334
Links to Exploration step
pubmed:27902334Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The NS1 protein of avian influenza virus H9N2 induces oxidative-stress-mediated chicken oviduct epithelial cells apoptosis.</title><author><name sortKey="Qi, Xuefeng" sort="Qi, Xuefeng" uniqKey="Qi X" first="Xuefeng" last="Qi">Xuefeng Qi</name><affiliation><nlm:affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Huizhu" sort="Zhang, Huizhu" uniqKey="Zhang H" first="Huizhu" last="Zhang">Huizhu Zhang</name><affiliation><nlm:affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Qiuzhen" sort="Wang, Qiuzhen" uniqKey="Wang Q" first="Qiuzhen" last="Wang">Qiuzhen Wang</name><affiliation><nlm:affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Jingyu" sort="Wang, Jingyu" uniqKey="Wang J" first="Jingyu" last="Wang">Jingyu Wang</name><affiliation><nlm:affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27902334</idno><idno type="pmid">27902334</idno><idno type="doi">10.1099/jgv.0.000625</idno><idno type="wicri:Area/PubMed/Corpus">000334</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000334</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The NS1 protein of avian influenza virus H9N2 induces oxidative-stress-mediated chicken oviduct epithelial cells apoptosis.</title><author><name sortKey="Qi, Xuefeng" sort="Qi, Xuefeng" uniqKey="Qi X" first="Xuefeng" last="Qi">Xuefeng Qi</name><affiliation><nlm:affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Huizhu" sort="Zhang, Huizhu" uniqKey="Zhang H" first="Huizhu" last="Zhang">Huizhu Zhang</name><affiliation><nlm:affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Qiuzhen" sort="Wang, Qiuzhen" uniqKey="Wang Q" first="Qiuzhen" last="Wang">Qiuzhen Wang</name><affiliation><nlm:affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Jingyu" sort="Wang, Jingyu" uniqKey="Wang J" first="Jingyu" last="Wang">Jingyu Wang</name><affiliation><nlm:affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="eISSN">1465-2099</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Caspases (metabolism)</term><term>Chickens</term><term>Epithelial Cells (cytology)</term><term>Epithelial Cells (metabolism)</term><term>Epithelial Cells (virology)</term><term>Female</term><term>Influenza A Virus, H9N2 Subtype (genetics)</term><term>Influenza A Virus, H9N2 Subtype (metabolism)</term><term>Influenza in Birds (metabolism)</term><term>Influenza in Birds (physiopathology)</term><term>Influenza in Birds (virology)</term><term>Mitochondria (metabolism)</term><term>Oviducts (cytology)</term><term>Oviducts (metabolism)</term><term>Oviducts (virology)</term><term>Oxidative Stress</term><term>Poultry Diseases (metabolism)</term><term>Poultry Diseases (physiopathology)</term><term>Poultry Diseases (virology)</term><term>Reactive Oxygen Species (metabolism)</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Caspases</term><term>Reactive Oxygen Species</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Epithelial Cells</term><term>Oviducts</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H9N2 Subtype</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Epithelial Cells</term><term>Influenza A Virus, H9N2 Subtype</term><term>Influenza in Birds</term><term>Mitochondria</term><term>Oviducts</term><term>Poultry Diseases</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Influenza in Birds</term><term>Poultry Diseases</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term><term>Influenza in Birds</term><term>Oviducts</term><term>Poultry Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Chickens</term><term>Female</term><term>Oxidative Stress</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pathogenesis of H9N2 subtype avian influenza virus infection (AIV) in hens is often related to oviduct tissue damage. The viral non-structural NS1 protein is thought to play a key role in regulating the pathogenicity of AIV, but its exact function in this process remains elusive. In this study, the pro-apoptosis effect of H9N2 NS1 protein was examined on chicken oviduct epithelial cells (COECs) and our data indicated that NS1-induced oxidative stress was a contributing factor in apoptosis. Our data indicate that NS1 protein level was correlated with reactive oxygen species (ROS) in COECs transfected with NS1 expression plasmids. Interestingly, decreased activities of antioxidant enzymes, superoxide dismutase and catalase, were observed in NS1-transfected COECs. Treatment of COECs with antioxidants, such as pyrrolidine dithiocarbamate (PDTC) or N-acetylcysteine (NAC), significantly inhibited NS1-induced apoptosis. Moreover, although antioxidant treatment has little effect on the activation of caspase-8 in NS1-transfected cells, the activation of caspase-3/9 and Bax/Bcl-2 were significantly downregulated. Taken together, the results of our study demonstrated that expression of H9N2 NS1 alone is sufficient to trigger oxidative stress in COECs. Additionally, NS1 protein can induce cellular apoptosis via activating ROS accumulation and mitochondria-mediated apoptotic signalling in COECs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27902334</PMID><DateCompleted><Year>2017</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1465-2099</ISSN><JournalIssue CitedMedium="Internet"><Volume>97</Volume><Issue>12</Issue><PubDate><Year>2016</Year><Month>Dec</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J. Gen. Virol.</ISOAbbreviation></Journal><ArticleTitle>The NS1 protein of avian influenza virus H9N2 induces oxidative-stress-mediated chicken oviduct epithelial cells apoptosis.</ArticleTitle><Pagination><MedlinePgn>3183-3192</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/jgv.0.000625</ELocationID><Abstract><AbstractText>The pathogenesis of H9N2 subtype avian influenza virus infection (AIV) in hens is often related to oviduct tissue damage. The viral non-structural NS1 protein is thought to play a key role in regulating the pathogenicity of AIV, but its exact function in this process remains elusive. In this study, the pro-apoptosis effect of H9N2 NS1 protein was examined on chicken oviduct epithelial cells (COECs) and our data indicated that NS1-induced oxidative stress was a contributing factor in apoptosis. Our data indicate that NS1 protein level was correlated with reactive oxygen species (ROS) in COECs transfected with NS1 expression plasmids. Interestingly, decreased activities of antioxidant enzymes, superoxide dismutase and catalase, were observed in NS1-transfected COECs. Treatment of COECs with antioxidants, such as pyrrolidine dithiocarbamate (PDTC) or N-acetylcysteine (NAC), significantly inhibited NS1-induced apoptosis. Moreover, although antioxidant treatment has little effect on the activation of caspase-8 in NS1-transfected cells, the activation of caspase-3/9 and Bax/Bcl-2 were significantly downregulated. Taken together, the results of our study demonstrated that expression of H9N2 NS1 alone is sufficient to trigger oxidative stress in COECs. Additionally, NS1 protein can induce cellular apoptosis via activating ROS accumulation and mitochondria-mediated apoptotic signalling in COECs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qi</LastName><ForeName>Xuefeng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Huizhu</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Qiuzhen</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jingyu</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Veterinary Medicine College, Northwest A&F University, Yangling 712100, PR China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>10</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C085820">INS1 protein, influenza virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017361">Viral Nonstructural Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="D020169">Caspases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="Y">Apoptosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020169" MajorTopicYN="N">Caspases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004847" MajorTopicYN="N">Epithelial Cells</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053127" MajorTopicYN="N">Influenza A Virus, H9N2 Subtype</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005585" MajorTopicYN="N">Influenza in Birds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000503" MajorTopicYN="N">physiopathology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008928" MajorTopicYN="N">Mitochondria</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010057" MajorTopicYN="N">Oviducts</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="Y">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011201" MajorTopicYN="N">Poultry Diseases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000503" MajorTopicYN="N">physiopathology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017361" MajorTopicYN="N">Viral Nonstructural Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>12</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>5</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>12</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27902334</ArticleId><ArticleId IdType="doi">10.1099/jgv.0.000625</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/StressCovidV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000334 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000334 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= StressCovidV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:27902334
|texte= The NS1 protein of avian influenza virus H9N2 induces oxidative-stress-mediated chicken oviduct epithelial cells apoptosis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:27902334" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a StressCovidV1
| This area was generated with Dilib version V0.6.33. |  |