Proteomic analysis of chicken embryo fibroblast cells infected with recombinant H5N1 avian influenza viruses with and without NS1 eIF4GI binding domain.
Identifieur interne : 000277 ( PubMed/Corpus ); précédent : 000276; suivant : 000278Proteomic analysis of chicken embryo fibroblast cells infected with recombinant H5N1 avian influenza viruses with and without NS1 eIF4GI binding domain.
Auteurs : Kelei Guo ; Xian Lin ; Yongtao Li ; Wei Qian ; Zhong Zou ; Huanchun Chen ; Hongbo Zhou ; Meilin JinSource :
- Oncotarget [ 1949-2553 ] ; 2018.
Abstract
Non-structural 1 (NS1) protein is a key virulence factor that regulates replication of influenza virus. A recombinant H5N1 virus lacking the eIF4GI-binding domain of NS1 (rNS1-SD30) exhibits significantly lower pathogenicity than H5N1 virus with an intact eIF4GI-binding domain (rNS1-wt). To further investigate this phenomenon, we performed comparative proteomics analyses to profile host proteins in chicken embryo fibroblasts (CEFs) infected with rNS1-wt and rNS1-SD30 viruses. In total, 81 differentially expressed (DE) proteins were identified at 12, 24, and 36 h post-infection. These proteins are mainly involved in the cytoskeletal, apoptotic and stress responses, transcription regulation, transport and metabolic processes, mRNA processing and splicing, and cellular signal transduction. Overexpression of DE proteins revealed that ANXA7 suppresses propagation of rNS1-SD30, but not rNS1-wt viruses. Moreover, ALDH7A1, ANXA7, and DCTN2 strongly enhanced IFN-β promoter activity induced by chicken MDA5 (chMDA5), and in the case of ANXA7, also by the rNS1-SD30 viral strain. NS1-wt co-transfection suppressed the ANXA7-mediated increase in IFN-β promoter activity induced by chMDA5. These findings highlight the role of NS1 eIF4GI binding domain in H5N1 pathogenicity, and may contribute to the design of antiviral strategies to reduce the high morbidity and mortality associated with this pathogen.
DOI: 10.18632/oncotarget.23615
PubMed: 29492200
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China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yongtao" sort="Li, Yongtao" uniqKey="Li Y" first="Yongtao" last="Li">Yongtao Li</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Qian, Wei" sort="Qian, Wei" uniqKey="Qian W" first="Wei" last="Qian">Wei Qian</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Zou, Zhong" sort="Zou, Zhong" uniqKey="Zou Z" first="Zhong" last="Zou">Zhong Zou</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Huanchun" sort="Chen, Huanchun" uniqKey="Chen H" first="Huanchun" last="Chen">Huanchun Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Hongbo" sort="Zhou, Hongbo" uniqKey="Zhou H" first="Hongbo" last="Zhou">Hongbo Zhou</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Jin, Meilin" sort="Jin, Meilin" uniqKey="Jin M" first="Meilin" last="Jin">Meilin Jin</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29492200</idno><idno type="pmid">29492200</idno><idno type="doi">10.18632/oncotarget.23615</idno><idno type="wicri:Area/PubMed/Corpus">000277</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000277</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Proteomic analysis of chicken embryo fibroblast cells infected with recombinant H5N1 avian influenza viruses with and without NS1 eIF4GI binding domain.</title><author><name sortKey="Guo, Kelei" sort="Guo, Kelei" uniqKey="Guo K" first="Kelei" last="Guo">Kelei Guo</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Lin, Xian" sort="Lin, Xian" uniqKey="Lin X" first="Xian" last="Lin">Xian Lin</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yongtao" sort="Li, Yongtao" uniqKey="Li Y" first="Yongtao" last="Li">Yongtao Li</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Qian, Wei" sort="Qian, Wei" uniqKey="Qian W" first="Wei" last="Qian">Wei Qian</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Zou, Zhong" sort="Zou, Zhong" uniqKey="Zou Z" first="Zhong" last="Zou">Zhong Zou</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Huanchun" sort="Chen, Huanchun" uniqKey="Chen H" first="Huanchun" last="Chen">Huanchun Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Hongbo" sort="Zhou, Hongbo" uniqKey="Zhou H" first="Hongbo" last="Zhou">Hongbo Zhou</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Jin, Meilin" sort="Jin, Meilin" uniqKey="Jin M" first="Meilin" last="Jin">Meilin Jin</name><affiliation><nlm:affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Oncotarget</title><idno type="eISSN">1949-2553</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Non-structural 1 (NS1) protein is a key virulence factor that regulates replication of influenza virus. A recombinant H5N1 virus lacking the eIF4GI-binding domain of NS1 (rNS1-SD30) exhibits significantly lower pathogenicity than H5N1 virus with an intact eIF4GI-binding domain (rNS1-wt). To further investigate this phenomenon, we performed comparative proteomics analyses to profile host proteins in chicken embryo fibroblasts (CEFs) infected with rNS1-wt and rNS1-SD30 viruses. In total, 81 differentially expressed (DE) proteins were identified at 12, 24, and 36 h post-infection. These proteins are mainly involved in the cytoskeletal, apoptotic and stress responses, transcription regulation, transport and metabolic processes, mRNA processing and splicing, and cellular signal transduction. Overexpression of DE proteins revealed that ANXA7 suppresses propagation of rNS1-SD30, but not rNS1-wt viruses. Moreover, ALDH7A1, ANXA7, and DCTN2 strongly enhanced IFN-β promoter activity induced by chicken MDA5 (chMDA5), and in the case of ANXA7, also by the rNS1-SD30 viral strain. NS1-wt co-transfection suppressed the ANXA7-mediated increase in IFN-β promoter activity induced by chMDA5. These findings highlight the role of NS1 eIF4GI binding domain in H5N1 pathogenicity, and may contribute to the design of antiviral strategies to reduce the high morbidity and mortality associated with this pathogen.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29492200</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1949-2553</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>9</Issue><PubDate><Year>2018</Year><Month>Feb</Month><Day>02</Day></PubDate></JournalIssue><Title>Oncotarget</Title><ISOAbbreviation>Oncotarget</ISOAbbreviation></Journal><ArticleTitle>Proteomic analysis of chicken embryo fibroblast cells infected with recombinant H5N1 avian influenza viruses with and without NS1 eIF4GI binding domain.</ArticleTitle><Pagination><MedlinePgn>8350-8367</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.18632/oncotarget.23615</ELocationID><Abstract><AbstractText>Non-structural 1 (NS1) protein is a key virulence factor that regulates replication of influenza virus. A recombinant H5N1 virus lacking the eIF4GI-binding domain of NS1 (rNS1-SD30) exhibits significantly lower pathogenicity than H5N1 virus with an intact eIF4GI-binding domain (rNS1-wt). To further investigate this phenomenon, we performed comparative proteomics analyses to profile host proteins in chicken embryo fibroblasts (CEFs) infected with rNS1-wt and rNS1-SD30 viruses. In total, 81 differentially expressed (DE) proteins were identified at 12, 24, and 36 h post-infection. These proteins are mainly involved in the cytoskeletal, apoptotic and stress responses, transcription regulation, transport and metabolic processes, mRNA processing and splicing, and cellular signal transduction. Overexpression of DE proteins revealed that ANXA7 suppresses propagation of rNS1-SD30, but not rNS1-wt viruses. Moreover, ALDH7A1, ANXA7, and DCTN2 strongly enhanced IFN-β promoter activity induced by chicken MDA5 (chMDA5), and in the case of ANXA7, also by the rNS1-SD30 viral strain. NS1-wt co-transfection suppressed the ANXA7-mediated increase in IFN-β promoter activity induced by chMDA5. These findings highlight the role of NS1 eIF4GI binding domain in H5N1 pathogenicity, and may contribute to the design of antiviral strategies to reduce the high morbidity and mortality associated with this pathogen.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Kelei</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Xian</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yongtao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qian</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zou</LastName><ForeName>Zhong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Huanchun</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Hongbo</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Meilin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, P. R. China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Oncotarget</MedlineTA><NlmUniqueID>101532965</NlmUniqueID><ISSNLinking>1949-2553</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">H5N1 avian influenza viruses</Keyword><Keyword MajorTopicYN="N">NS1 eIF4GI binding domain</Keyword><Keyword MajorTopicYN="N">chicken embryo fibroblast</Keyword><Keyword MajorTopicYN="N">differentially expressed proteins</Keyword><Keyword MajorTopicYN="N">proteomic analysis</Keyword></KeywordList><CoiStatement>CONFLICTS OF INTEREST The authors declare that there are no potential conflicts of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>07</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>10</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29492200</ArticleId><ArticleId IdType="doi">10.18632/oncotarget.23615</ArticleId><ArticleId IdType="pii">23615</ArticleId><ArticleId IdType="pmc">PMC5823584</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Biol Chem. 2002 Nov 22;277(47):45306-14</Citation><ArticleIdList><ArticleId 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