Subcellular proteomic analysis of human host cells infected with H3N2 swine influenza virus.
Identifieur interne : 000602 ( Ncbi/Merge ); précédent : 000601; suivant : 000603Subcellular proteomic analysis of human host cells infected with H3N2 swine influenza virus.
Auteurs : Xiaopeng Wu [République populaire de Chine] ; Sanying Wang ; Yang Yu ; Jinyang Zhang ; Zeyu Sun ; Yan Yan ; Jiyong ZhouSource :
- Proteomics [ 1615-9861 ] ; 2013.
Descripteurs français
- KwdFr :
- Données de séquences moléculaires, Espace intracellulaire (), Espace intracellulaire (métabolisme), Espace intracellulaire (virologie), Humains, Interactions hôte-pathogène (physiologie), Lignée cellulaire, Microscopie confocale, Protéines (), Protéines (analyse), Protéines (métabolisme), Protéome (), Protéome (analyse), Protéome (métabolisme), Sous-type H3N2 du virus de la grippe A (physiologie), Spectrométrie de masse, Séquence d'acides aminés, Technique de Western, Transcriptome, Électrophorèse bidimensionnelle sur gel.
- MESH :
- analyse : Protéines, Protéome.
- métabolisme : Espace intracellulaire, Protéines, Protéome.
- physiologie : Interactions hôte-pathogène, Sous-type H3N2 du virus de la grippe A.
- virologie : Espace intracellulaire.
- Données de séquences moléculaires, Espace intracellulaire, Humains, Lignée cellulaire, Microscopie confocale, Protéines, Protéome, Spectrométrie de masse, Séquence d'acides aminés, Technique de Western, Transcriptome, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Amino Acid Sequence, Blotting, Western, Cell Line, Electrophoresis, Gel, Two-Dimensional, Host-Pathogen Interactions (physiology), Humans, Influenza A Virus, H3N2 Subtype (physiology), Intracellular Space (chemistry), Intracellular Space (metabolism), Intracellular Space (virology), Mass Spectrometry, Microscopy, Confocal, Molecular Sequence Data, Proteins (analysis), Proteins (chemistry), Proteins (classification), Proteins (metabolism), Proteome (analysis), Proteome (chemistry), Proteome (metabolism), Transcriptome.
- MESH :
- chemical , analysis : Proteins, Proteome.
- chemistry : Intracellular Space, Proteins, Proteome.
- chemical , classification : Proteins.
- metabolism : Intracellular Space, Proteins, Proteome.
- physiology : Host-Pathogen Interactions, Influenza A Virus, H3N2 Subtype.
- virology : Intracellular Space.
- Amino Acid Sequence, Blotting, Western, Cell Line, Electrophoresis, Gel, Two-Dimensional, Humans, Mass Spectrometry, Microscopy, Confocal, Molecular Sequence Data, Transcriptome.
Abstract
Cross-species transmissions of swine influenza viruses (SIVs) raise great public health concerns. In this study, subcellular proteomic profiles of human A549 cells inoculated with H3N2 subtype SIV were used to characterize dynamic cellular responses to infection. By 2DE and MS, 27 differentially expressed (13 upregulated, 14 downregulated) cytoplasmic proteins and 20 differentially expressed (13 upregulated, 7 downregulated) nuclear proteins were identified. Gene ontology analysis suggested that these differentially expressed proteins were mainly involved in cell death, stress response, lipid metabolism, cell signaling, and RNA PTMs. Moreover, 25 corresponding genes of the differentially expressed proteins were quantitated by real time RT-PCR to examine the transcriptional profiles between mock- and virus-infected A549 cells. Western blot analysis confirmed that changes in abundance of identified cellular proteins heterogeneous nuclear ribonucleoprotein (hnRNP) U, hnRNP C, ALDH1A1, tryptophanyl-tRNA synthetase, IFI35, and HSPB1 in H3N2 SIV-infected cells were consistent with results of 2DE analysis. By confocal microscopy, nucleus-to-cytoplasm translocation of hnRNP C and colocalization between the viral nonstructural protein 1 and hnRNP C as well as N-myc (and STAT) interactor were observed upon infection. Ingenuity Pathway Analysis revealed that cellular proteins altered during infection were grouped mainly into NFκB and interferon signaling networks. Collectively, these identified subcellular constituents provide an important framework for understanding host/SIV interactions and underlying mechanisms of SIV cross-species infection and pathogenesis.
DOI: 10.1002/pmic.201300180
PubMed: 24115376
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China.</nlm:affiliation><orgName type="university">Université de Zhejiang</orgName><country>République populaire de Chine</country><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Wang, Sanying" sort="Wang, Sanying" uniqKey="Wang S" first="Sanying" last="Wang">Sanying Wang</name></author><author><name sortKey="Yu, Yang" sort="Yu, Yang" uniqKey="Yu Y" first="Yang" last="Yu">Yang Yu</name></author><author><name sortKey="Zhang, Jinyang" sort="Zhang, Jinyang" uniqKey="Zhang J" first="Jinyang" last="Zhang">Jinyang Zhang</name></author><author><name sortKey="Sun, Zeyu" sort="Sun, Zeyu" uniqKey="Sun Z" first="Zeyu" last="Sun">Zeyu Sun</name></author><author><name sortKey="Yan, Yan" sort="Yan, Yan" uniqKey="Yan Y" first="Yan" last="Yan">Yan Yan</name></author><author><name sortKey="Zhou, Jiyong" sort="Zhou, Jiyong" uniqKey="Zhou J" first="Jiyong" last="Zhou">Jiyong Zhou</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:24115376</idno><idno type="pmid">24115376</idno><idno type="doi">10.1002/pmic.201300180</idno><idno type="wicri:Area/PubMed/Corpus">000505</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000505</idno><idno type="wicri:Area/PubMed/Curation">000503</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000503</idno><idno type="wicri:Area/PubMed/Checkpoint">000513</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000513</idno><idno type="wicri:Area/Ncbi/Merge">000602</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Subcellular proteomic analysis of human host cells infected with H3N2 swine influenza virus.</title><author><name sortKey="Wu, Xiaopeng" sort="Wu, Xiaopeng" uniqKey="Wu X" first="Xiaopeng" last="Wu">Xiaopeng Wu</name><affiliation wicri:level="4"><nlm:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, P. R. China; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou, P. R. 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Zhou</name></author></analytic><series><title level="j">Proteomics</title><idno type="eISSN">1615-9861</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Blotting, Western</term><term>Cell Line</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Host-Pathogen Interactions (physiology)</term><term>Humans</term><term>Influenza A Virus, H3N2 Subtype (physiology)</term><term>Intracellular Space (chemistry)</term><term>Intracellular Space (metabolism)</term><term>Intracellular Space (virology)</term><term>Mass Spectrometry</term><term>Microscopy, Confocal</term><term>Molecular Sequence Data</term><term>Proteins (analysis)</term><term>Proteins (chemistry)</term><term>Proteins (classification)</term><term>Proteins (metabolism)</term><term>Proteome (analysis)</term><term>Proteome (chemistry)</term><term>Proteome 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type="abstract" xml:lang="en">Cross-species transmissions of swine influenza viruses (SIVs) raise great public health concerns. In this study, subcellular proteomic profiles of human A549 cells inoculated with H3N2 subtype SIV were used to characterize dynamic cellular responses to infection. By 2DE and MS, 27 differentially expressed (13 upregulated, 14 downregulated) cytoplasmic proteins and 20 differentially expressed (13 upregulated, 7 downregulated) nuclear proteins were identified. Gene ontology analysis suggested that these differentially expressed proteins were mainly involved in cell death, stress response, lipid metabolism, cell signaling, and RNA PTMs. Moreover, 25 corresponding genes of the differentially expressed proteins were quantitated by real time RT-PCR to examine the transcriptional profiles between mock- and virus-infected A549 cells. Western blot analysis confirmed that changes in abundance of identified cellular proteins heterogeneous nuclear ribonucleoprotein (hnRNP) U, hnRNP C, ALDH1A1, tryptophanyl-tRNA synthetase, IFI35, and HSPB1 in H3N2 SIV-infected cells were consistent with results of 2DE analysis. By confocal microscopy, nucleus-to-cytoplasm translocation of hnRNP C and colocalization between the viral nonstructural protein 1 and hnRNP C as well as N-myc (and STAT) interactor were observed upon infection. Ingenuity Pathway Analysis revealed that cellular proteins altered during infection were grouped mainly into NFκB and interferon signaling networks. Collectively, these identified subcellular constituents provide an important framework for understanding host/SIV interactions and underlying mechanisms of SIV cross-species infection and pathogenesis. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24115376</PMID><DateCompleted><Year>2014</Year><Month>06</Month><Day>30</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>22</Issue><PubDate><Year>2013</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>Subcellular proteomic analysis of human host cells infected with H3N2 swine influenza virus.</ArticleTitle><Pagination><MedlinePgn>3309-26</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.201300180</ELocationID><Abstract><AbstractText>Cross-species transmissions of swine influenza viruses (SIVs) raise great public health concerns. In this study, subcellular proteomic profiles of human A549 cells inoculated with H3N2 subtype SIV were used to characterize dynamic cellular responses to infection. By 2DE and MS, 27 differentially expressed (13 upregulated, 14 downregulated) cytoplasmic proteins and 20 differentially expressed (13 upregulated, 7 downregulated) nuclear proteins were identified. Gene ontology analysis suggested that these differentially expressed proteins were mainly involved in cell death, stress response, lipid metabolism, cell signaling, and RNA PTMs. Moreover, 25 corresponding genes of the differentially expressed proteins were quantitated by real time RT-PCR to examine the transcriptional profiles between mock- and virus-infected A549 cells. Western blot analysis confirmed that changes in abundance of identified cellular proteins heterogeneous nuclear ribonucleoprotein (hnRNP) U, hnRNP C, ALDH1A1, tryptophanyl-tRNA synthetase, IFI35, and HSPB1 in H3N2 SIV-infected cells were consistent with results of 2DE analysis. By confocal microscopy, nucleus-to-cytoplasm translocation of hnRNP C and colocalization between the viral nonstructural protein 1 and hnRNP C as well as N-myc (and STAT) interactor were observed upon infection. Ingenuity Pathway Analysis revealed that cellular proteins altered during infection were grouped mainly into NFκB and interferon signaling networks. Collectively, these identified subcellular constituents provide an important framework for understanding host/SIV interactions and underlying mechanisms of SIV cross-species infection and pathogenesis. </AbstractText><CopyrightInformation>© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Xiaopeng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, P. R. China; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Sanying</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Yang</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jinyang</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Zeyu</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Jiyong</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>10</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Proteomics</MedlineTA><NlmUniqueID>101092707</NlmUniqueID><ISSNLinking>1615-9853</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053122" MajorTopicYN="N">Influenza A Virus, H3N2 Subtype</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042541" MajorTopicYN="N">Intracellular Space</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">2DE</Keyword><Keyword MajorTopicYN="N">A549 cells</Keyword><Keyword MajorTopicYN="N">Interaction</Keyword><Keyword MajorTopicYN="N">Microbiology</Keyword><Keyword MajorTopicYN="N">Subcellular proteomics</Keyword><Keyword MajorTopicYN="N">Swine influenza</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>05</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>08</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>08</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>10</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>10</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>7</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24115376</ArticleId><ArticleId IdType="doi">10.1002/pmic.201300180</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><noCountry><name sortKey="Sun, Zeyu" sort="Sun, Zeyu" uniqKey="Sun Z" first="Zeyu" last="Sun">Zeyu Sun</name><name sortKey="Wang, Sanying" sort="Wang, Sanying" uniqKey="Wang S" first="Sanying" last="Wang">Sanying Wang</name><name sortKey="Yan, Yan" sort="Yan, Yan" uniqKey="Yan Y" first="Yan" last="Yan">Yan Yan</name><name sortKey="Yu, Yang" sort="Yu, Yang" uniqKey="Yu Y" first="Yang" last="Yu">Yang Yu</name><name sortKey="Zhang, Jinyang" sort="Zhang, Jinyang" uniqKey="Zhang J" first="Jinyang" last="Zhang">Jinyang Zhang</name><name sortKey="Zhou, Jiyong" sort="Zhou, Jiyong" uniqKey="Zhou J" first="Jiyong" last="Zhou">Jiyong Zhou</name></noCountry><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Wu, Xiaopeng" sort="Wu, Xiaopeng" uniqKey="Wu X" first="Xiaopeng" last="Wu">Xiaopeng Wu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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