Expression of the sarA family of genes in different strains of Staphylococcus aureus.
Identifieur interne : 000311 ( Ncbi/Curation ); précédent : 000310; suivant : 000312Expression of the sarA family of genes in different strains of Staphylococcus aureus.
Auteurs : Anand Ballal [États-Unis] ; Adhar C. Manna [États-Unis]Source :
- Microbiology (Reading, England) [ 1350-0872 ] ; 2009.
Descripteurs français
- KwdFr :
- ARN bactérien (analyse), ARN bactérien (génétique), Analyse de profil d'expression de gènes, Facteurs de transcription (biosynthèse), Facteurs de transcription (génétique), Famille multigénique, Humains, Infections à staphylocoques (microbiologie), Protéines bactériennes (biosynthèse), Protéines bactériennes (génétique), Protéines de liaison à l'ADN (biosynthèse), Protéines de liaison à l'ADN (génétique), Protéines de répression (biosynthèse), Protéines de répression (génétique), Régulation de l'expression des gènes bactériens, Staphylococcus aureus (génétique), Staphylococcus aureus (métabolisme), Technique de Western, Transactivateurs (biosynthèse), Transactivateurs (génétique), Transcription génétique.
- MESH :
- analyse : ARN bactérien.
- biosynthèse : Facteurs de transcription, Protéines bactériennes, Protéines de liaison à l'ADN, Protéines de répression, Transactivateurs.
- génétique : ARN bactérien, Facteurs de transcription, Protéines bactériennes, Protéines de liaison à l'ADN, Protéines de répression, Staphylococcus aureus, Transactivateurs.
- microbiologie : Infections à staphylocoques.
- métabolisme : Staphylococcus aureus.
- Analyse de profil d'expression de gènes, Famille multigénique, Humains, Régulation de l'expression des gènes bactériens, Technique de Western, Transcription génétique.
English descriptors
- KwdEn :
- Bacterial Proteins (biosynthesis), Bacterial Proteins (genetics), Blotting, Western, DNA-Binding Proteins (biosynthesis), DNA-Binding Proteins (genetics), Gene Expression Profiling, Gene Expression Regulation, Bacterial, Humans, Multigene Family, RNA, Bacterial (analysis), RNA, Bacterial (genetics), Repressor Proteins (biosynthesis), Repressor Proteins (genetics), Staphylococcal Infections (microbiology), Staphylococcus aureus (genetics), Staphylococcus aureus (metabolism), Trans-Activators (biosynthesis), Trans-Activators (genetics), Transcription Factors (biosynthesis), Transcription Factors (genetics), Transcription, Genetic.
- MESH :
- chemical , analysis : RNA, Bacterial.
- chemical , biosynthesis : Bacterial Proteins, DNA-Binding Proteins, Repressor Proteins, Trans-Activators, Transcription Factors.
- chemical , genetics : Bacterial Proteins, DNA-Binding Proteins, RNA, Bacterial, Repressor Proteins, Trans-Activators, Transcription Factors.
- genetics : Staphylococcus aureus.
- metabolism : Staphylococcus aureus.
- microbiology : Staphylococcal Infections.
- Blotting, Western, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Humans, Multigene Family, Transcription, Genetic.
Abstract
Expression of genes involved in the pathogenesis of Staphylococcus aureus is controlled by global regulatory loci, including two-component regulatory systems and transcriptional regulators. The staphylococcal-specific SarA family of transcription regulators control large numbers of target genes involved in virulence, autolysis, biofilm formation, stress responses and metabolic processes, and are recognized as potential therapeutic targets. Expression of some of these important regulators has been examined, mostly in laboratory strains, while the pattern of expression of these genes in other strains, especially clinical isolates, is largely unknown. In this report, a comparative analysis of 10 sarA-family genes was conducted in six different S. aureus strains, including two laboratory (RN6390, SH1000) and four clinical (MW2, Newman, COL and UAMS-1) strains, by Northern and Western blot analyses. Transcription of most of the sarA-family genes showed a strong growth phase-dependence in all strains tested. Among these genes, no difference was observed in expression of the sarA, sarV, sarT and sarU genes, while a major difference was observed in expression of the sarX gene only in strain RN6390. Expression of mgrA, rot, sarZ, sarR and sarS was observed in all strains, but the level of expression varied from strain to strain.
DOI: 10.1099/mic.0.027417-0
PubMed: 19389785
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pubmed:19389785Le document en format XML
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<term>Facteurs de transcription (biosynthèse)</term>
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<term>Trans-Activators</term>
<term>Transcription Factors</term>
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<term>Protéines bactériennes</term>
<term>Protéines de liaison à l'ADN</term>
<term>Protéines de répression</term>
<term>Transactivateurs</term>
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<term>Famille multigénique</term>
<term>Humains</term>
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<front><div type="abstract" xml:lang="en">Expression of genes involved in the pathogenesis of Staphylococcus aureus is controlled by global regulatory loci, including two-component regulatory systems and transcriptional regulators. The staphylococcal-specific SarA family of transcription regulators control large numbers of target genes involved in virulence, autolysis, biofilm formation, stress responses and metabolic processes, and are recognized as potential therapeutic targets. Expression of some of these important regulators has been examined, mostly in laboratory strains, while the pattern of expression of these genes in other strains, especially clinical isolates, is largely unknown. In this report, a comparative analysis of 10 sarA-family genes was conducted in six different S. aureus strains, including two laboratory (RN6390, SH1000) and four clinical (MW2, Newman, COL and UAMS-1) strains, by Northern and Western blot analyses. Transcription of most of the sarA-family genes showed a strong growth phase-dependence in all strains tested. Among these genes, no difference was observed in expression of the sarA, sarV, sarT and sarU genes, while a major difference was observed in expression of the sarX gene only in strain RN6390. Expression of mgrA, rot, sarZ, sarR and sarS was observed in all strains, but the level of expression varied from strain to strain.</div>
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