Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein
Identifieur interne : 000607 ( Ncbi/Checkpoint ); précédent : 000606; suivant : 000608Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein
Auteurs : Tsuey-Ching Yang ; Shih-Wein Li ; Chien-Chen Lai ; Kai-Zen Lu ; Man-Tzu Chiu ; Tsung-Han Hsieh ; Lei Wan ; Cheng-Wen LinSource :
- Proteomics [ 1615-9853 ] ; 2013.
Abstract
Japanese encephalitis virus (
Url:
DOI: 10.1002/pmic.201300001
PubMed: 24166946
PubMed Central: 7167617
Affiliations:
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PMC:7167617Le document en format XML
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ype <styled-content style="fixed-case" toggle="no">I</styled-content>
interferon antagonism of <styled-content style="fixed-case" toggle="no">J</styled-content>
apanese encephalitis virus <styled-content style="fixed-case" toggle="no">NS</styled-content>
5 protein</title>
<author><name sortKey="Yang, Tsuey Hing" sort="Yang, Tsuey Hing" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name>
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<author><name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Proteomic analysis for <styled-content style="fixed-case" toggle="no">T</styled-content>
ype <styled-content style="fixed-case" toggle="no">I</styled-content>
interferon antagonism of <styled-content style="fixed-case" toggle="no">J</styled-content>
apanese encephalitis virus <styled-content style="fixed-case" toggle="no">NS</styled-content>
5 protein</title>
<author><name sortKey="Yang, Tsuey Hing" sort="Yang, Tsuey Hing" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name>
<affiliation><nlm:aff id="pmic7588-aff-0001"></nlm:aff>
</affiliation>
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<author><name sortKey="Li, Shih Ein" sort="Li, Shih Ein" uniqKey="Li S" first="Shih-Wein" last="Li">Shih-Wein Li</name>
<affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff>
</affiliation>
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<author><name sortKey="Lai, Chien Hen" sort="Lai, Chien Hen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
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<author><name sortKey="Lu, Kai En" sort="Lu, Kai En" uniqKey="Lu K" first="Kai-Zen" last="Lu">Kai-Zen Lu</name>
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<author><name sortKey="Hsieh, Tsung An" sort="Hsieh, Tsung An" uniqKey="Hsieh T" first="Tsung-Han" last="Hsieh">Tsung-Han Hsieh</name>
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<author><name sortKey="Wan, Lei" sort="Wan, Lei" uniqKey="Wan L" first="Lei" last="Wan">Lei Wan</name>
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<author><name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
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<series><title level="j">Proteomics</title>
<idno type="ISSN">1615-9853</idno>
<idno type="eISSN">1615-9861</idno>
<imprint><date when="2013">2013</date>
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<front><div type="abstract" xml:lang="en"><p>Japanese encephalitis virus (<styled-content style="fixed-case" toggle="no">JEV</styled-content>
) nonstructural protein 5 (<styled-content style="fixed-case" toggle="no">NS</styled-content>
5) exhibits a <styled-content style="fixed-case" toggle="no">T</styled-content>
ype <styled-content style="fixed-case" toggle="no">I</styled-content>
interferon (<styled-content style="fixed-case" toggle="no">IFN</styled-content>
) antagonistic function. This study characterizes Type I <styled-content style="fixed-case" toggle="no">IFN</styled-content>
antagonism mechanism of <styled-content style="fixed-case" toggle="no">NS</styled-content>
5 protein, using proteomic approach. In human neuroblastoma cells, <styled-content style="fixed-case" toggle="no">NS</styled-content>
5 expression would suppress <styled-content style="fixed-case" toggle="no">IFN</styled-content>
β‐induced responses, for example, expression of <styled-content style="fixed-case" toggle="no">IFN</styled-content>
‐stimulated genes <styled-content style="fixed-case" toggle="no">PKR</styled-content>
and <styled-content style="fixed-case" toggle="no">OAS</styled-content>
as well as <styled-content style="fixed-case" toggle="no">STAT</styled-content>
1 nuclear translocation and phosphorylation. Proteomic analysis showed <styled-content style="fixed-case" toggle="no">JEV NS</styled-content>
5 downregulating calreticulin, while upregulating cyclophilin <styled-content style="fixed-case" toggle="no">A</styled-content>
, <styled-content style="fixed-case" toggle="no">HSP</styled-content>
60 and stress‐induced‐phosphoprotein 1. Gene silence of calreticulin raised intracellular <styled-content style="fixed-case" toggle="no">C</styled-content>
a<sup>2+</sup>
levels while inhibiting nuclear translocalization of <styled-content style="fixed-case" toggle="no">STAT</styled-content>
1 and <styled-content style="fixed-case" toggle="no">NFAT</styled-content>
‐1 in response to <styled-content style="fixed-case" toggle="no">IFN</styled-content>
β, thus, indicating calreticulin downregulation linked with <styled-content style="fixed-case" toggle="no">T</styled-content>
ype <styled-content style="fixed-case" toggle="no">I IFN</styled-content>
antagonism of <styled-content style="fixed-case" toggle="no">JEV NS</styled-content>
5 via activation of Ca<sup>2+</sup>
/calicineurin. Calcineurin inhibitor cyclosporin A attenuated NS5‐mediated inhibition of <styled-content style="fixed-case" toggle="no">IFN</styled-content>
β‐induced responses, for example, <styled-content style="fixed-case" toggle="no">IFN</styled-content>
‐sensitive response element driven luciferase, <styled-content style="fixed-case" toggle="no">STAT</styled-content>
1‐dependent <styled-content style="fixed-case" toggle="no">PKR</styled-content>
m<styled-content style="fixed-case" toggle="no">RNA</styled-content>
expression, as well as phosphorylation and nuclear translocation of <styled-content style="fixed-case" toggle="no">STAT</styled-content>
1. Transfection with calcineurin (vs. control) si<styled-content style="fixed-case" toggle="no">RNA</styled-content>
enhanced nuclear translocalization of <styled-content style="fixed-case" toggle="no">STAT</styled-content>
1 and upregulated <styled-content style="fixed-case" toggle="no">PKR</styled-content>
expression in <styled-content style="fixed-case" toggle="no">NS</styled-content>
5‐expressing cells in response to <styled-content style="fixed-case" toggle="no">IFN</styled-content>
β. Results prove <styled-content style="fixed-case" toggle="no">C</styled-content>
a<sup>2+</sup>
, calreticulin, and calcineurin involvement in <styled-content style="fixed-case" toggle="no">STAT</styled-content>
1‐mediated signaling as well as a key role of <styled-content style="fixed-case" toggle="no">JEV NS</styled-content>
5 in <styled-content style="fixed-case" toggle="no">T</styled-content>
ype <styled-content style="fixed-case" toggle="no">I IFN</styled-content>
antagonism. This study offers insights into the molecular mechanism of <styled-content style="fixed-case" toggle="no">T</styled-content>
ype <styled-content style="fixed-case" toggle="no">I</styled-content>
interferon antagonism by <styled-content style="fixed-case" toggle="no">JEV NS</styled-content>
5.</p>
</div>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Chiu, Man Zu" sort="Chiu, Man Zu" uniqKey="Chiu M" first="Man-Tzu" last="Chiu">Man-Tzu Chiu</name>
<name sortKey="Hsieh, Tsung An" sort="Hsieh, Tsung An" uniqKey="Hsieh T" first="Tsung-Han" last="Hsieh">Tsung-Han Hsieh</name>
<name sortKey="Lai, Chien Hen" sort="Lai, Chien Hen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
<name sortKey="Li, Shih Ein" sort="Li, Shih Ein" uniqKey="Li S" first="Shih-Wein" last="Li">Shih-Wein Li</name>
<name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
<name sortKey="Lu, Kai En" sort="Lu, Kai En" uniqKey="Lu K" first="Kai-Zen" last="Lu">Kai-Zen Lu</name>
<name sortKey="Wan, Lei" sort="Wan, Lei" uniqKey="Wan L" first="Lei" last="Wan">Lei Wan</name>
<name sortKey="Yang, Tsuey Hing" sort="Yang, Tsuey Hing" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name>
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