Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein
Identifieur interne : 000607 ( Ncbi/Checkpoint ); précédent : 000606; suivant : 000608Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein
Auteurs : Tsuey-Ching Yang ; Shih-Wein Li ; Chien-Chen Lai ; Kai-Zen Lu ; Man-Tzu Chiu ; Tsung-Han Hsieh ; Lei Wan ; Cheng-Wen LinSource :
- Proteomics [ 1615-9853 ] ; 2013.
Abstract
Japanese encephalitis virus (
Url:
DOI: 10.1002/pmic.201300001
PubMed: 24166946
PubMed Central: 7167617
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Proteomic analysis for <styled-content style="fixed-case" toggle="no">T</styled-content>ype <styled-content style="fixed-case" toggle="no">I</styled-content> interferon antagonism of <styled-content style="fixed-case" toggle="no">J</styled-content>apanese encephalitis virus <styled-content style="fixed-case" toggle="no">NS</styled-content>5 protein</title><author><name sortKey="Yang, Tsuey Hing" sort="Yang, Tsuey Hing" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name><affiliation><nlm:aff id="pmic7588-aff-0001"></nlm:aff></affiliation></author><author><name sortKey="Li, Shih Ein" sort="Li, Shih Ein" uniqKey="Li S" first="Shih-Wein" last="Li">Shih-Wein Li</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation></author><author><name sortKey="Lai, Chien Hen" sort="Lai, Chien Hen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name><affiliation><nlm:aff id="pmic7588-aff-0003"></nlm:aff></affiliation><affiliation><nlm:aff id="pmic7588-aff-0004"></nlm:aff></affiliation></author><author><name sortKey="Lu, Kai En" sort="Lu, Kai En" uniqKey="Lu K" first="Kai-Zen" last="Lu">Kai-Zen Lu</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation></author><author><name sortKey="Chiu, Man Zu" sort="Chiu, Man Zu" uniqKey="Chiu M" first="Man-Tzu" last="Chiu">Man-Tzu Chiu</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation></author><author><name sortKey="Hsieh, Tsung An" sort="Hsieh, Tsung An" uniqKey="Hsieh T" first="Tsung-Han" last="Hsieh">Tsung-Han Hsieh</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation></author><author><name sortKey="Wan, Lei" sort="Wan, Lei" uniqKey="Wan L" first="Lei" last="Wan">Lei Wan</name><affiliation><nlm:aff id="pmic7588-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="pmic7588-aff-0005"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24166946</idno><idno type="pmc">7167617</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167617</idno><idno type="RBID">PMC:7167617</idno><idno type="doi">10.1002/pmic.201300001</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000798</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000798</idno><idno type="wicri:Area/Pmc/Curation">000798</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000798</idno><idno type="wicri:Area/Pmc/Checkpoint">000834</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000834</idno><idno type="wicri:Area/Ncbi/Merge">000607</idno><idno type="wicri:Area/Ncbi/Curation">000607</idno><idno type="wicri:Area/Ncbi/Checkpoint">000607</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Proteomic analysis for <styled-content style="fixed-case" toggle="no">T</styled-content>ype <styled-content style="fixed-case" toggle="no">I</styled-content> interferon antagonism of <styled-content style="fixed-case" toggle="no">J</styled-content>apanese encephalitis virus <styled-content style="fixed-case" toggle="no">NS</styled-content>5 protein</title><author><name sortKey="Yang, Tsuey Hing" sort="Yang, Tsuey Hing" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name><affiliation><nlm:aff id="pmic7588-aff-0001"></nlm:aff></affiliation></author><author><name sortKey="Li, Shih Ein" sort="Li, Shih Ein" uniqKey="Li S" first="Shih-Wein" last="Li">Shih-Wein Li</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation></author><author><name sortKey="Lai, Chien Hen" sort="Lai, Chien Hen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name><affiliation><nlm:aff id="pmic7588-aff-0003"></nlm:aff></affiliation><affiliation><nlm:aff id="pmic7588-aff-0004"></nlm:aff></affiliation></author><author><name sortKey="Lu, Kai En" sort="Lu, Kai En" uniqKey="Lu K" first="Kai-Zen" last="Lu">Kai-Zen Lu</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation></author><author><name sortKey="Chiu, Man Zu" sort="Chiu, Man Zu" uniqKey="Chiu M" first="Man-Tzu" last="Chiu">Man-Tzu Chiu</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation></author><author><name sortKey="Hsieh, Tsung An" sort="Hsieh, Tsung An" uniqKey="Hsieh T" first="Tsung-Han" last="Hsieh">Tsung-Han Hsieh</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation></author><author><name sortKey="Wan, Lei" sort="Wan, Lei" uniqKey="Wan L" first="Lei" last="Wan">Lei Wan</name><affiliation><nlm:aff id="pmic7588-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Lin, Cheng En" sort="Lin, Cheng En" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name><affiliation><nlm:aff id="pmic7588-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="pmic7588-aff-0005"></nlm:aff></affiliation></author></analytic><series><title level="j">Proteomics</title><idno type="ISSN">1615-9853</idno><idno type="eISSN">1615-9861</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Japanese encephalitis virus (<styled-content style="fixed-case" toggle="no">JEV</styled-content>) nonstructural protein 5 (<styled-content style="fixed-case" toggle="no">NS</styled-content>5) exhibits a <styled-content style="fixed-case" toggle="no">T</styled-content>ype <styled-content style="fixed-case" toggle="no">I</styled-content> interferon (<styled-content style="fixed-case" toggle="no">IFN</styled-content>) antagonistic function. This study characterizes Type I <styled-content style="fixed-case" toggle="no">IFN</styled-content> antagonism mechanism of <styled-content style="fixed-case" toggle="no">NS</styled-content>5 protein, using proteomic approach. In human neuroblastoma cells, <styled-content style="fixed-case" toggle="no">NS</styled-content>5 expression would suppress <styled-content style="fixed-case" toggle="no">IFN</styled-content>β‐induced responses, for example, expression of <styled-content style="fixed-case" toggle="no">IFN</styled-content>‐stimulated genes <styled-content style="fixed-case" toggle="no">PKR</styled-content> and <styled-content style="fixed-case" toggle="no">OAS</styled-content> as well as <styled-content style="fixed-case" toggle="no">STAT</styled-content>1 nuclear translocation and phosphorylation. Proteomic analysis showed <styled-content style="fixed-case" toggle="no">JEV NS</styled-content>5 downregulating calreticulin, while upregulating cyclophilin <styled-content style="fixed-case" toggle="no">A</styled-content>, <styled-content style="fixed-case" toggle="no">HSP</styled-content> 60 and stress‐induced‐phosphoprotein 1. Gene silence of calreticulin raised intracellular <styled-content style="fixed-case" toggle="no">C</styled-content>a<sup>2+</sup> levels while inhibiting nuclear translocalization of <styled-content style="fixed-case" toggle="no">STAT</styled-content>1 and <styled-content style="fixed-case" toggle="no">NFAT</styled-content>‐1 in response to <styled-content style="fixed-case" toggle="no">IFN</styled-content>β, thus, indicating calreticulin downregulation linked with <styled-content style="fixed-case" toggle="no">T</styled-content>ype <styled-content style="fixed-case" toggle="no">I IFN</styled-content> antagonism of <styled-content style="fixed-case" toggle="no">JEV NS</styled-content>5 via activation of Ca<sup>2+</sup>/calicineurin. Calcineurin inhibitor cyclosporin A attenuated NS5‐mediated inhibition of <styled-content style="fixed-case" toggle="no">IFN</styled-content>β‐induced responses, for example, <styled-content style="fixed-case" toggle="no">IFN</styled-content>‐sensitive response element driven luciferase, <styled-content style="fixed-case" toggle="no">STAT</styled-content>1‐dependent <styled-content style="fixed-case" toggle="no">PKR</styled-content> m<styled-content style="fixed-case" toggle="no">RNA</styled-content> expression, as well as phosphorylation and nuclear translocation of <styled-content style="fixed-case" toggle="no">STAT</styled-content>1. Transfection with calcineurin (vs. control) si<styled-content style="fixed-case" toggle="no">RNA</styled-content> enhanced nuclear translocalization of <styled-content style="fixed-case" toggle="no">STAT</styled-content>1 and upregulated <styled-content style="fixed-case" toggle="no">PKR</styled-content> expression in <styled-content style="fixed-case" toggle="no">NS</styled-content>5‐expressing cells in response to <styled-content style="fixed-case" toggle="no">IFN</styled-content>β. Results prove <styled-content style="fixed-case" toggle="no">C</styled-content>a<sup>2+</sup>, calreticulin, and calcineurin involvement in <styled-content style="fixed-case" toggle="no">STAT</styled-content>1‐mediated signaling as well as a key role of <styled-content style="fixed-case" toggle="no">JEV NS</styled-content>5 in <styled-content style="fixed-case" toggle="no">T</styled-content>ype <styled-content style="fixed-case" toggle="no">I IFN</styled-content> antagonism. This study offers insights into the molecular mechanism of <styled-content style="fixed-case" toggle="no">T</styled-content>ype <styled-content style="fixed-case" toggle="no">I</styled-content> interferon antagonism by <styled-content style="fixed-case" toggle="no">JEV NS</styled-content>5.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Fields, B N" uniqKey="Fields B">B. N. Fields</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Knipe, D M" uniqKey="Knipe D">D. M. Knipe</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Saluzzo, J F" uniqKey="Saluzzo J">J. F. 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