2-GHz band CW and W-CDMA modulated radiofrequency fields have no significant effect on cell proliferation and gene expression profile in human cells.
Identifieur interne : 000361 ( Ncbi/Checkpoint ); précédent : 000360; suivant : 0003622-GHz band CW and W-CDMA modulated radiofrequency fields have no significant effect on cell proliferation and gene expression profile in human cells.
Auteurs : Masaru Sekijima [Japon] ; Hiroshi Takeda ; Katsuaki Yasunaga ; Noriko Sakuma ; Hideki Hirose ; Toshio Nojima ; Junji MiyakoshiSource :
- Journal of radiation research [ 1349-9157 ] ; 2010.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes, Facteurs temps, Fibroblastes (métabolisme), Génome humain, Humains, Lignée cellulaire tumorale, Micro-ondes, Ondes hertziennes, Prolifération cellulaire, Protéines du choc thermique (métabolisme), Survie cellulaire, Séquençage par oligonucléotides en batterie, Tumeurs (métabolisme), Évolution de la maladie.
- MESH :
- métabolisme : Fibroblastes, Protéines du choc thermique, Tumeurs.
- Analyse de profil d'expression de gènes, Facteurs temps, Génome humain, Humains, Lignée cellulaire tumorale, Micro-ondes, Ondes hertziennes, Prolifération cellulaire, Survie cellulaire, Séquençage par oligonucléotides en batterie, Évolution de la maladie.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Heat-Shock Proteins.
- metabolism : Fibroblasts, Neoplasms.
- Cell Line, Tumor, Cell Phone, Cell Proliferation, Cell Survival, Disease Progression, Gene Expression Profiling, Genome, Human, Humans, Microwaves, Oligonucleotide Array Sequence Analysis, Radio Waves, Time Factors.
Abstract
We investigated the mechanisms by which radiofrequency (RF) fields exert their activity, and the changes in both cell proliferation and the gene expression profile in the human cell lines, A172 (glioblastoma), H4 (neuroglioma), and IMR-90 (fibroblasts from normal fetal lung) following exposure to 2.1425 GHz continuous wave (CW) and Wideband Code Division Multiple Access (W-CDMA) RF fields at three field levels. During the incubation phase, cells were exposed at the specific absorption rates (SARs) of 80, 250, or 800 mW/kg with both CW and W-CDMA RF fields for up to 96 h. Heat shock treatment was used as the positive control. No significant differences in cell growth or viability were observed between any test group exposed to W-CDMA or CW radiation and the sham-exposed negative controls. Using the Affymetrix Human Genome Array, only a very small (< 1%) number of available genes (ca. 16,000 to 19,000) exhibited altered expression in each experiment. The results confirm that low-level exposure to 2.1425 GHz CW and W-CDMA RF fields for up to 96 h did not act as an acute cytotoxicant in either cell proliferation or the gene expression profile. These results suggest that RF exposure up to the limit of whole-body average SAR levels as specified in the ICNIRP guidelines is unlikely to elicit a general stress response in the tested cell lines under these conditions.
DOI: 10.1269/jrr.09126
PubMed: 20215713
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000708
- to stream PubMed, to step Curation: 000705
- to stream PubMed, to step Checkpoint: 000702
- to stream Ncbi, to step Merge: 000361
- to stream Ncbi, to step Curation: 000361
Links to Exploration step
pubmed:20215713Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">2-GHz band CW and W-CDMA modulated radiofrequency fields have no significant effect on cell proliferation and gene expression profile in human cells.</title>
<author><name sortKey="Sekijima, Masaru" sort="Sekijima, Masaru" uniqKey="Sekijima M" first="Masaru" last="Sekijima">Masaru Sekijima</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Medical Science Research Center, Mitsubishi Chemical Medience Corporation, Kamisu, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Advanced Medical Science Research Center, Mitsubishi Chemical Medience Corporation, Kamisu</wicri:regionArea>
<wicri:noRegion>Kamisu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Takeda, Hiroshi" sort="Takeda, Hiroshi" uniqKey="Takeda H" first="Hiroshi" last="Takeda">Hiroshi Takeda</name>
</author>
<author><name sortKey="Yasunaga, Katsuaki" sort="Yasunaga, Katsuaki" uniqKey="Yasunaga K" first="Katsuaki" last="Yasunaga">Katsuaki Yasunaga</name>
</author>
<author><name sortKey="Sakuma, Noriko" sort="Sakuma, Noriko" uniqKey="Sakuma N" first="Noriko" last="Sakuma">Noriko Sakuma</name>
</author>
<author><name sortKey="Hirose, Hideki" sort="Hirose, Hideki" uniqKey="Hirose H" first="Hideki" last="Hirose">Hideki Hirose</name>
</author>
<author><name sortKey="Nojima, Toshio" sort="Nojima, Toshio" uniqKey="Nojima T" first="Toshio" last="Nojima">Toshio Nojima</name>
</author>
<author><name sortKey="Miyakoshi, Junji" sort="Miyakoshi, Junji" uniqKey="Miyakoshi J" first="Junji" last="Miyakoshi">Junji Miyakoshi</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2010">2010</date>
<idno type="RBID">pubmed:20215713</idno>
<idno type="pmid">20215713</idno>
<idno type="doi">10.1269/jrr.09126</idno>
<idno type="wicri:Area/PubMed/Corpus">000708</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000708</idno>
<idno type="wicri:Area/PubMed/Curation">000705</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000705</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000702</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000702</idno>
<idno type="wicri:Area/Ncbi/Merge">000361</idno>
<idno type="wicri:Area/Ncbi/Curation">000361</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000361</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">2-GHz band CW and W-CDMA modulated radiofrequency fields have no significant effect on cell proliferation and gene expression profile in human cells.</title>
<author><name sortKey="Sekijima, Masaru" sort="Sekijima, Masaru" uniqKey="Sekijima M" first="Masaru" last="Sekijima">Masaru Sekijima</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Medical Science Research Center, Mitsubishi Chemical Medience Corporation, Kamisu, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Advanced Medical Science Research Center, Mitsubishi Chemical Medience Corporation, Kamisu</wicri:regionArea>
<wicri:noRegion>Kamisu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Takeda, Hiroshi" sort="Takeda, Hiroshi" uniqKey="Takeda H" first="Hiroshi" last="Takeda">Hiroshi Takeda</name>
</author>
<author><name sortKey="Yasunaga, Katsuaki" sort="Yasunaga, Katsuaki" uniqKey="Yasunaga K" first="Katsuaki" last="Yasunaga">Katsuaki Yasunaga</name>
</author>
<author><name sortKey="Sakuma, Noriko" sort="Sakuma, Noriko" uniqKey="Sakuma N" first="Noriko" last="Sakuma">Noriko Sakuma</name>
</author>
<author><name sortKey="Hirose, Hideki" sort="Hirose, Hideki" uniqKey="Hirose H" first="Hideki" last="Hirose">Hideki Hirose</name>
</author>
<author><name sortKey="Nojima, Toshio" sort="Nojima, Toshio" uniqKey="Nojima T" first="Toshio" last="Nojima">Toshio Nojima</name>
</author>
<author><name sortKey="Miyakoshi, Junji" sort="Miyakoshi, Junji" uniqKey="Miyakoshi J" first="Junji" last="Miyakoshi">Junji Miyakoshi</name>
</author>
</analytic>
<series><title level="j">Journal of radiation research</title>
<idno type="eISSN">1349-9157</idno>
<imprint><date when="2010" type="published">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Line, Tumor</term>
<term>Cell Phone</term>
<term>Cell Proliferation</term>
<term>Cell Survival</term>
<term>Disease Progression</term>
<term>Fibroblasts (metabolism)</term>
<term>Gene Expression Profiling</term>
<term>Genome, Human</term>
<term>Heat-Shock Proteins (metabolism)</term>
<term>Humans</term>
<term>Microwaves</term>
<term>Neoplasms (metabolism)</term>
<term>Oligonucleotide Array Sequence Analysis</term>
<term>Radio Waves</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Facteurs temps</term>
<term>Fibroblastes (métabolisme)</term>
<term>Génome humain</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
<term>Micro-ondes</term>
<term>Ondes hertziennes</term>
<term>Prolifération cellulaire</term>
<term>Protéines du choc thermique (métabolisme)</term>
<term>Survie cellulaire</term>
<term>Séquençage par oligonucléotides en batterie</term>
<term>Tumeurs (métabolisme)</term>
<term>Évolution de la maladie</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Heat-Shock Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fibroblasts</term>
<term>Neoplasms</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fibroblastes</term>
<term>Protéines du choc thermique</term>
<term>Tumeurs</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term>
<term>Cell Phone</term>
<term>Cell Proliferation</term>
<term>Cell Survival</term>
<term>Disease Progression</term>
<term>Gene Expression Profiling</term>
<term>Genome, Human</term>
<term>Humans</term>
<term>Microwaves</term>
<term>Oligonucleotide Array Sequence Analysis</term>
<term>Radio Waves</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Facteurs temps</term>
<term>Génome humain</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
<term>Micro-ondes</term>
<term>Ondes hertziennes</term>
<term>Prolifération cellulaire</term>
<term>Survie cellulaire</term>
<term>Séquençage par oligonucléotides en batterie</term>
<term>Évolution de la maladie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We investigated the mechanisms by which radiofrequency (RF) fields exert their activity, and the changes in both cell proliferation and the gene expression profile in the human cell lines, A172 (glioblastoma), H4 (neuroglioma), and IMR-90 (fibroblasts from normal fetal lung) following exposure to 2.1425 GHz continuous wave (CW) and Wideband Code Division Multiple Access (W-CDMA) RF fields at three field levels. During the incubation phase, cells were exposed at the specific absorption rates (SARs) of 80, 250, or 800 mW/kg with both CW and W-CDMA RF fields for up to 96 h. Heat shock treatment was used as the positive control. No significant differences in cell growth or viability were observed between any test group exposed to W-CDMA or CW radiation and the sham-exposed negative controls. Using the Affymetrix Human Genome Array, only a very small (< 1%) number of available genes (ca. 16,000 to 19,000) exhibited altered expression in each experiment. The results confirm that low-level exposure to 2.1425 GHz CW and W-CDMA RF fields for up to 96 h did not act as an acute cytotoxicant in either cell proliferation or the gene expression profile. These results suggest that RF exposure up to the limit of whole-body average SAR levels as specified in the ICNIRP guidelines is unlikely to elicit a general stress response in the tested cell lines under these conditions.</div>
</front>
</TEI>
<affiliations><list><country><li>Japon</li>
</country>
</list>
<tree><noCountry><name sortKey="Hirose, Hideki" sort="Hirose, Hideki" uniqKey="Hirose H" first="Hideki" last="Hirose">Hideki Hirose</name>
<name sortKey="Miyakoshi, Junji" sort="Miyakoshi, Junji" uniqKey="Miyakoshi J" first="Junji" last="Miyakoshi">Junji Miyakoshi</name>
<name sortKey="Nojima, Toshio" sort="Nojima, Toshio" uniqKey="Nojima T" first="Toshio" last="Nojima">Toshio Nojima</name>
<name sortKey="Sakuma, Noriko" sort="Sakuma, Noriko" uniqKey="Sakuma N" first="Noriko" last="Sakuma">Noriko Sakuma</name>
<name sortKey="Takeda, Hiroshi" sort="Takeda, Hiroshi" uniqKey="Takeda H" first="Hiroshi" last="Takeda">Hiroshi Takeda</name>
<name sortKey="Yasunaga, Katsuaki" sort="Yasunaga, Katsuaki" uniqKey="Yasunaga K" first="Katsuaki" last="Yasunaga">Katsuaki Yasunaga</name>
</noCountry>
<country name="Japon"><noRegion><name sortKey="Sekijima, Masaru" sort="Sekijima, Masaru" uniqKey="Sekijima M" first="Masaru" last="Sekijima">Masaru Sekijima</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/StressCovidV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000361 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000361 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= StressCovidV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= pubmed:20215713 |texte= 2-GHz band CW and W-CDMA modulated radiofrequency fields have no significant effect on cell proliferation and gene expression profile in human cells. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:20215713" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a StressCovidV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |