Analysis of HK-2 cells exposed to oxalate and calcium oxalate crystals: proteomic insights into the molecular mechanisms of renal injury and stone formation
Identifieur interne : 001C32 ( Main/Merge ); précédent : 001C31; suivant : 001C33Analysis of HK-2 cells exposed to oxalate and calcium oxalate crystals: proteomic insights into the molecular mechanisms of renal injury and stone formation
Auteurs : Shushang Chen [République populaire de Chine] ; Xiaofeng Gao [République populaire de Chine] ; Yinghao Sun [République populaire de Chine] ; Chuanliang Xu [République populaire de Chine] ; Linhui Wang [République populaire de Chine] ; Tie Zhou [République populaire de Chine]Source :
- Urological Research [ 0300-5623 ] ; 2010-02-01.
English descriptors
Abstract
Abstract: Exposure to high levels of oxalate and calcium oxalate monohydrate (COM) crystals is injurious to renal epithelial cells and triggers serial responses related to stone formation. Multiple molecules and proteins are involved in this process, but previous studies have generally been limited, without an overall understanding of protein expression alteration after oxalate and/or crystal exposure as well as its role in stone formation. We used proteomic analysis to reveal the changes in the proteome of HK-2 cells induced by oxalate and COM crystals, so as to provide candidate proteins involved in the molecular mechanisms concerning HK-2 cell injury and kidney stone formation. HK-2 cells were exposed to oxalate plus COM crystals at different concentrations in various samples. Cell viability was determined using a Cell Counting Kit-8 assay kit. For proteomic analysis, cells were exposed to oxalate (2 mM) and COM crystals (200 ug/ml) for 12 h. The proteins were separated by two-dimensional electrophoresis and the differentially expressed proteins were identified by liquid chromatography electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS). Validation of protein expression was further performed by Western blot analysis. Oxalate and COM crystals showed concentration-dependent toxicity on HK-2 cells. A total of 12 differentially expressed proteins in HK-2 cells induced by oxalate and COM crystals were identified, which were involved in various aspects of cellular processes. Our study provides a platform for further studying the molecular mechanism of renal epithelial cell injury and kidney stone formation.
Url:
DOI: 10.1007/s00240-009-0226-0
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000D46
- to stream Istex, to step Curation: 000D23
- to stream Istex, to step Checkpoint: 000A36
Links to Exploration step
ISTEX:02FBF39BD0729786B7EE4B03A4C51DB281E2E004Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analysis of HK-2 cells exposed to oxalate and calcium oxalate crystals: proteomic insights into the molecular mechanisms of renal injury and stone formation</title><author><name sortKey="Chen, Shushang" sort="Chen, Shushang" uniqKey="Chen S" first="Shushang" last="Chen">Shushang Chen</name></author><author><name sortKey="Gao, Xiaofeng" sort="Gao, Xiaofeng" uniqKey="Gao X" first="Xiaofeng" last="Gao">Xiaofeng Gao</name></author><author><name sortKey="Sun, Yinghao" sort="Sun, Yinghao" uniqKey="Sun Y" first="Yinghao" last="Sun">Yinghao Sun</name></author><author><name sortKey="Xu, Chuanliang" sort="Xu, Chuanliang" uniqKey="Xu C" first="Chuanliang" last="Xu">Chuanliang Xu</name></author><author><name sortKey="Wang, Linhui" sort="Wang, Linhui" uniqKey="Wang L" first="Linhui" last="Wang">Linhui Wang</name></author><author><name sortKey="Zhou, Tie" sort="Zhou, Tie" uniqKey="Zhou T" first="Tie" last="Zhou">Tie Zhou</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:02FBF39BD0729786B7EE4B03A4C51DB281E2E004</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1007/s00240-009-0226-0</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-JCKZ4C0W-K/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000D46</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000D46</idno><idno type="wicri:Area/Istex/Curation">000D23</idno><idno type="wicri:Area/Istex/Checkpoint">000A36</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000A36</idno><idno type="wicri:doubleKey">0300-5623:2009:Chen S:analysis:of:hk</idno><idno type="wicri:Area/Main/Merge">001C32</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Analysis of HK-2 cells exposed to oxalate and calcium oxalate crystals: proteomic insights into the molecular mechanisms of renal injury and stone formation</title><author><name sortKey="Chen, Shushang" sort="Chen, Shushang" uniqKey="Chen S" first="Shushang" last="Chen">Shushang Chen</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Urology, Changhai Hospital of the Second Military Medical University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Urology, Fuzhou General Hospital, Nanjing Military Area Command of Chinese PLA, Fuzhou</wicri:regionArea><placeName><settlement type="city">Fuzhou</settlement><region type="province">Fujian</region></placeName></affiliation></author><author><name sortKey="Gao, Xiaofeng" sort="Gao, Xiaofeng" uniqKey="Gao X" first="Xiaofeng" last="Gao">Xiaofeng Gao</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Urology, Changhai Hospital of the Second Military Medical University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Yinghao" sort="Sun, Yinghao" uniqKey="Sun Y" first="Yinghao" last="Sun">Yinghao Sun</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Urology, Changhai Hospital of the Second Military Medical University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Xu, Chuanliang" sort="Xu, Chuanliang" uniqKey="Xu C" first="Chuanliang" last="Xu">Chuanliang Xu</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Urology, Changhai Hospital of the Second Military Medical University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Linhui" sort="Wang, Linhui" uniqKey="Wang L" first="Linhui" last="Wang">Linhui Wang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Urology, Changhai Hospital of the Second Military Medical University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Tie" sort="Zhou, Tie" uniqKey="Zhou T" first="Tie" last="Zhou">Tie Zhou</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Urology, Changhai Hospital of the Second Military Medical University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Urological Research</title><title level="j" type="sub">Urolithiasis</title><title level="j" type="abbrev">Urol Res</title><idno type="ISSN">0300-5623</idno><idno type="eISSN">1434-0879</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2010-02-01">2010-02-01</date><biblScope unit="volume">38</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="7">7</biblScope><biblScope unit="page" to="15">15</biblScope></imprint><idno type="ISSN">0300-5623</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-5623</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Calcium oxalate monohydrate</term><term>Nephrolithiasis</term><term>Oxalate</term><term>Proteomics</term><term>Renal epithelial cell</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Exposure to high levels of oxalate and calcium oxalate monohydrate (COM) crystals is injurious to renal epithelial cells and triggers serial responses related to stone formation. Multiple molecules and proteins are involved in this process, but previous studies have generally been limited, without an overall understanding of protein expression alteration after oxalate and/or crystal exposure as well as its role in stone formation. We used proteomic analysis to reveal the changes in the proteome of HK-2 cells induced by oxalate and COM crystals, so as to provide candidate proteins involved in the molecular mechanisms concerning HK-2 cell injury and kidney stone formation. HK-2 cells were exposed to oxalate plus COM crystals at different concentrations in various samples. Cell viability was determined using a Cell Counting Kit-8 assay kit. For proteomic analysis, cells were exposed to oxalate (2 mM) and COM crystals (200 ug/ml) for 12 h. The proteins were separated by two-dimensional electrophoresis and the differentially expressed proteins were identified by liquid chromatography electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS). Validation of protein expression was further performed by Western blot analysis. Oxalate and COM crystals showed concentration-dependent toxicity on HK-2 cells. A total of 12 differentially expressed proteins in HK-2 cells induced by oxalate and COM crystals were identified, which were involved in various aspects of cellular processes. Our study provides a platform for further studying the molecular mechanism of renal epithelial cell injury and kidney stone formation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/StressCovidV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C32 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001C32 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= StressCovidV1
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:02FBF39BD0729786B7EE4B03A4C51DB281E2E004
|texte= Analysis of HK-2 cells exposed to oxalate and calcium oxalate crystals: proteomic insights into the molecular mechanisms of renal injury and stone formation
}}
| This area was generated with Dilib version V0.6.33. |  |