The NS1 protein of influenza A virus interacts with cellular processing bodies and stress granules through RNA-associated protein 55 (RAP55) during virus infection.
Identifieur interne : 001290 ( Main/Merge ); précédent : 001289; suivant : 001291The NS1 protein of influenza A virus interacts with cellular processing bodies and stress granules through RNA-associated protein 55 (RAP55) during virus infection.
Auteurs : Bobo Wing-Yee Mok [République populaire de Chine] ; Wenjun Song ; Pui Wang ; Hung Tai ; Yixin Chen ; Min Zheng ; Xi Wen ; Siu-Ying Lau ; Wai Lan Wu ; Ken Matsumoto ; Kwok-Yung Yuen ; Honglin ChenSource :
- Journal of virology [ 1098-5514 ] ; 2012.
Descripteurs français
- KwdFr :
- Amorces ADN (génétique), Animaux, Cellules HEK293, Cellules rénales canines Madin-Darby, Chiens, Granulations cytoplasmiques (métabolisme), Humains, Immunoprécipitation, Interactions hôte-pathogène, Luciferases, Protéines virales non structurales (métabolisme), Ribonucléoprotéines (génétique), Ribonucléoprotéines (métabolisme), Réplication virale (physiologie), Sous-type H1N1 du virus de la grippe A (métabolisme), Sous-type H1N1 du virus de la grippe A (physiologie), Spectrométrie de masse MALDI, Spectrométrie de masse en tandem, Technique FISH, Technique d'immunofluorescence indirecte, Technique de Western, Techniques de knock-down de gènes, Vecteurs génétiques.
- MESH :
- génétique : Amorces ADN, Ribonucléoprotéines.
- métabolisme : Granulations cytoplasmiques, Protéines virales non structurales, Ribonucléoprotéines, Sous-type H1N1 du virus de la grippe A.
- physiologie : Réplication virale, Sous-type H1N1 du virus de la grippe A.
- Animaux, Cellules HEK293, Cellules rénales canines Madin-Darby, Chiens, Humains, Immunoprécipitation, Interactions hôte-pathogène, Luciferases, Spectrométrie de masse MALDI, Spectrométrie de masse en tandem, Technique FISH, Technique d'immunofluorescence indirecte, Technique de Western, Techniques de knock-down de gènes, Vecteurs génétiques.
English descriptors
- KwdEn :
- Animals, Blotting, Western, Cytoplasmic Granules (metabolism), DNA Primers (genetics), Dogs, Fluorescent Antibody Technique, Indirect, Gene Knockdown Techniques, Genetic Vectors, HEK293 Cells, Host-Pathogen Interactions, Humans, Immunoprecipitation, In Situ Hybridization, Fluorescence, Influenza A Virus, H1N1 Subtype (metabolism), Influenza A Virus, H1N1 Subtype (physiology), Luciferases, Madin Darby Canine Kidney Cells, Ribonucleoproteins (genetics), Ribonucleoproteins (metabolism), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry, Viral Nonstructural Proteins (metabolism), Virus Replication (physiology).
- MESH :
- chemical , genetics : DNA Primers, Ribonucleoproteins.
- metabolism : Cytoplasmic Granules, Influenza A Virus, H1N1 Subtype, Ribonucleoproteins, Viral Nonstructural Proteins.
- physiology : Influenza A Virus, H1N1 Subtype, Virus Replication.
- Animals, Blotting, Western, Dogs, Fluorescent Antibody Technique, Indirect, Gene Knockdown Techniques, Genetic Vectors, HEK293 Cells, Host-Pathogen Interactions, Humans, Immunoprecipitation, In Situ Hybridization, Fluorescence, Luciferases, Madin Darby Canine Kidney Cells, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry.
Abstract
The nonstructural protein (NS1) of influenza A virus performs multiple functions in the virus life cycle. Proteomic screening for cellular proteins which interact with NS1 identified the cellular protein RAP55, which is one of the components of cellular processing bodies (P-bodies) and stress granules. To verify whether NS1 interacts with cellular P-bodies, interactions between NS1, RAP55, and other P-body-associated proteins (Ago1, Ago2, and DCP1a) were confirmed using coimmunoprecipitation and cellular colocalization assays. Overexpression of RAP55 induced RAP55-associated stress granule formation and suppressed virus replication. Knockdown of RAP55 with small interfering RNA (siRNA) or expression of a dominant-negative mutant RAP55 protein with defective interaction with P-bodies blocked NS1 colocalization to P-bodies in cells. Expression of NS1 inhibited RAP55 expression and formation of RAP55-associated P-bodies/stress granules. The viral nucleoprotein (NP) was found to be targeted to stress granules in the absence of NS1 but localized to P-bodies when NS1 was coexpressed. Restriction of virus replication via P-bodies occurred in the early phases of infection, as the number of RAP55-associated P-bodies in cells diminished over the course of virus infection. NS1 interaction with RAP55-associated P-bodies/stress granules was associated with RNA binding and mediated via a protein kinase R (PKR)-interacting viral element. Mutations introduced into either RNA binding sites (R38 and K41) or PKR interaction sites (I123, M124, K126, and N127) caused NS1 proteins to lose the ability to interact with RAP55 and to inhibit stress granules. These results reveal an interplay between virus and host during virus replication in which NP is targeted to P-bodies/stress granules while NS1 counteracts this host restriction mechanism.
DOI: 10.1128/JVI.00647-12
PubMed: 22973032
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pubmed:22973032Le document en format XML
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sortKey="Song, Wenjun" sort="Song, Wenjun" uniqKey="Song W" first="Wenjun" last="Song">Wenjun Song</name></author><author><name sortKey="Wang, Pui" sort="Wang, Pui" uniqKey="Wang P" first="Pui" last="Wang">Pui Wang</name></author><author><name sortKey="Tai, Hung" sort="Tai, Hung" uniqKey="Tai H" first="Hung" last="Tai">Hung Tai</name></author><author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name></author><author><name sortKey="Zheng, Min" sort="Zheng, Min" uniqKey="Zheng M" first="Min" last="Zheng">Min Zheng</name></author><author><name sortKey="Wen, Xi" sort="Wen, Xi" uniqKey="Wen X" first="Xi" last="Wen">Xi Wen</name></author><author><name sortKey="Lau, Siu Ying" sort="Lau, Siu Ying" uniqKey="Lau S" first="Siu-Ying" last="Lau">Siu-Ying Lau</name></author><author><name sortKey="Wu, Wai Lan" sort="Wu, Wai Lan" uniqKey="Wu W" first="Wai Lan" last="Wu">Wai Lan Wu</name></author><author><name sortKey="Matsumoto, Ken" 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Diseases, Department of Microbiology, and the Research Center of Infection and Immunology, The University of Hong Kong, Hong Kong SAR</wicri:regionArea><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Song, Wenjun" sort="Song, Wenjun" uniqKey="Song W" first="Wenjun" last="Song">Wenjun Song</name></author><author><name sortKey="Wang, Pui" sort="Wang, Pui" uniqKey="Wang P" first="Pui" last="Wang">Pui Wang</name></author><author><name sortKey="Tai, Hung" sort="Tai, Hung" uniqKey="Tai H" first="Hung" last="Tai">Hung Tai</name></author><author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name></author><author><name sortKey="Zheng, Min" sort="Zheng, Min" uniqKey="Zheng M" first="Min" last="Zheng">Min Zheng</name></author><author><name sortKey="Wen, Xi" sort="Wen, Xi" uniqKey="Wen X" first="Xi" last="Wen">Xi Wen</name></author><author><name sortKey="Lau, Siu Ying" sort="Lau, Siu Ying" uniqKey="Lau S" first="Siu-Ying" last="Lau">Siu-Ying Lau</name></author><author><name sortKey="Wu, Wai Lan" sort="Wu, Wai Lan" uniqKey="Wu W" first="Wai Lan" last="Wu">Wai Lan Wu</name></author><author><name sortKey="Matsumoto, Ken" sort="Matsumoto, Ken" uniqKey="Matsumoto K" first="Ken" last="Matsumoto">Ken Matsumoto</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></author><author><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cytoplasmic Granules (metabolism)</term><term>DNA Primers (genetics)</term><term>Dogs</term><term>Fluorescent Antibody Technique, Indirect</term><term>Gene Knockdown Techniques</term><term>Genetic Vectors</term><term>HEK293 Cells</term><term>Host-Pathogen Interactions</term><term>Humans</term><term>Immunoprecipitation</term><term>In Situ Hybridization, Fluorescence</term><term>Influenza A Virus, H1N1 Subtype (metabolism)</term><term>Influenza A Virus, H1N1 Subtype (physiology)</term><term>Luciferases</term><term>Madin Darby Canine Kidney Cells</term><term>Ribonucleoproteins (genetics)</term><term>Ribonucleoproteins (metabolism)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term><term>Tandem Mass Spectrometry</term><term>Viral Nonstructural Proteins (metabolism)</term><term>Virus Replication (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (génétique)</term><term>Animaux</term><term>Cellules HEK293</term><term>Cellules rénales canines Madin-Darby</term><term>Chiens</term><term>Granulations cytoplasmiques (métabolisme)</term><term>Humains</term><term>Immunoprécipitation</term><term>Interactions hôte-pathogène</term><term>Luciferases</term><term>Protéines virales non structurales (métabolisme)</term><term>Ribonucléoprotéines (génétique)</term><term>Ribonucléoprotéines (métabolisme)</term><term>Réplication virale (physiologie)</term><term>Sous-type H1N1 du virus de la grippe A (métabolisme)</term><term>Sous-type H1N1 du virus de la grippe A (physiologie)</term><term>Spectrométrie de masse MALDI</term><term>Spectrométrie de masse en tandem</term><term>Technique FISH</term><term>Technique d'immunofluorescence indirecte</term><term>Technique de Western</term><term>Techniques de knock-down de gènes</term><term>Vecteurs génétiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term><term>Ribonucleoproteins</term></keywords><keywords scheme="MESH" 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Technique, Indirect</term><term>Gene Knockdown Techniques</term><term>Genetic Vectors</term><term>HEK293 Cells</term><term>Host-Pathogen Interactions</term><term>Humans</term><term>Immunoprecipitation</term><term>In Situ Hybridization, Fluorescence</term><term>Luciferases</term><term>Madin Darby Canine Kidney Cells</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HEK293</term><term>Cellules rénales canines Madin-Darby</term><term>Chiens</term><term>Humains</term><term>Immunoprécipitation</term><term>Interactions hôte-pathogène</term><term>Luciferases</term><term>Spectrométrie de masse MALDI</term><term>Spectrométrie de masse en tandem</term><term>Technique FISH</term><term>Technique d'immunofluorescence indirecte</term><term>Technique de Western</term><term>Techniques de knock-down de gènes</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The nonstructural protein (NS1) of influenza A virus performs multiple functions in the virus life cycle. Proteomic screening for cellular proteins which interact with NS1 identified the cellular protein RAP55, which is one of the components of cellular processing bodies (P-bodies) and stress granules. To verify whether NS1 interacts with cellular P-bodies, interactions between NS1, RAP55, and other P-body-associated proteins (Ago1, Ago2, and DCP1a) were confirmed using coimmunoprecipitation and cellular colocalization assays. Overexpression of RAP55 induced RAP55-associated stress granule formation and suppressed virus replication. Knockdown of RAP55 with small interfering RNA (siRNA) or expression of a dominant-negative mutant RAP55 protein with defective interaction with P-bodies blocked NS1 colocalization to P-bodies in cells. Expression of NS1 inhibited RAP55 expression and formation of RAP55-associated P-bodies/stress granules. The viral nucleoprotein (NP) was found to be targeted to stress granules in the absence of NS1 but localized to P-bodies when NS1 was coexpressed. Restriction of virus replication via P-bodies occurred in the early phases of infection, as the number of RAP55-associated P-bodies in cells diminished over the course of virus infection. NS1 interaction with RAP55-associated P-bodies/stress granules was associated with RNA binding and mediated via a protein kinase R (PKR)-interacting viral element. Mutations introduced into either RNA binding sites (R38 and K41) or PKR interaction sites (I123, M124, K126, and N127) caused NS1 proteins to lose the ability to interact with RAP55 and to inhibit stress granules. These results reveal an interplay between virus and host during virus replication in which NP is targeted to P-bodies/stress granules while NS1 counteracts this host restriction mechanism.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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