Serveur d'exploration Stress et Covid

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Label‐Free Quantitative Proteomic Analysis of Differentially Expressed Membrane Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain

Identifieur interne : 000A88 ( Main/Merge ); précédent : 000A87; suivant : 000A89

Label‐Free Quantitative Proteomic Analysis of Differentially Expressed Membrane Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain

Auteurs : Zehui Qu ; Fei Gao ; Liwei Li ; Yujiao Zhang ; Yifeng Jiang ; Lingxue Yu ; Yanjun Zhou ; Hao Zheng ; Wu Tong ; Guoxin Li ; Guangzhi Tong

Source :

RBID : PMC:6084361

Abstract

Abstract

Significant differences exist between the highly pathogenic (HP) porcine reproductive and respiratory syndrome virus (PRRSV) and its attenuated pathogenic (AP) strain in the ability to infect host cells. The mechanisms by which different virulent strains invade host cells remain relatively unknown. In this study, pulmonary alveolar macrophages (PAMs) are infected with HP‐PRRSV (HuN4) and AP‐PRRSV (HuN4‐F112) for 24 h, then harvested and subjected to label‐free quantitative MS. A total of 2849 proteins are identified, including 95 that are differentially expressed. Among them, 26 proteins are located on the membrane. The most differentially expressed proteins are involved in response to stimulus, metabolic process, and immune system process, which mainly have the function of binding and catalytic activity. Cluster of differentiation CD163, vimentin (VIM), and nmII as well as detected proteins are assessed together by string analysis, which elucidated a potentially different infection mechanism. According to the function annotations, PRRSV with different virulence may mainly differ in immunology, inflammation, immune evasion as well as cell apoptosis. This is the first attempt to explore the differential characteristics between HP‐PRRSV and its attenuated PRRSV infected PAMs focusing on membrane proteins which will be of great help to further understand the different infective mechanisms of HP‐PRRSV and AP‐PRRSV.


Url:
DOI: 10.1002/pmic.201700101
PubMed: 29052333
PubMed Central: 6084361

Links toward previous steps (curation, corpus...)


Links to Exploration step

PMC:6084361

Le document en format XML

<record>
<TEI>
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">Label‐Free Quantitative Proteomic Analysis of Differentially Expressed Membrane Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain</title>
<author>
<name sortKey="Qu, Zehui" sort="Qu, Zehui" uniqKey="Qu Z" first="Zehui" last="Qu">Zehui Qu</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Gao, Fei" sort="Gao, Fei" uniqKey="Gao F" first="Fei" last="Gao">Fei Gao</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Li, Liwei" sort="Li, Liwei" uniqKey="Li L" first="Liwei" last="Li">Liwei Li</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Zhang, Yujiao" sort="Zhang, Yujiao" uniqKey="Zhang Y" first="Yujiao" last="Zhang">Yujiao Zhang</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Jiang, Yifeng" sort="Jiang, Yifeng" uniqKey="Jiang Y" first="Yifeng" last="Jiang">Yifeng Jiang</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Yu, Lingxue" sort="Yu, Lingxue" uniqKey="Yu L" first="Lingxue" last="Yu">Lingxue Yu</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Zhou, Yanjun" sort="Zhou, Yanjun" uniqKey="Zhou Y" first="Yanjun" last="Zhou">Yanjun Zhou</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Zheng, Hao" sort="Zheng, Hao" uniqKey="Zheng H" first="Hao" last="Zheng">Hao Zheng</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Tong, Wu" sort="Tong, Wu" uniqKey="Tong W" first="Wu" last="Tong">Wu Tong</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Li, Guoxin" sort="Li, Guoxin" uniqKey="Li G" first="Guoxin" last="Li">Guoxin Li</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Tong, Guangzhi" sort="Tong, Guangzhi" uniqKey="Tong G" first="Guangzhi" last="Tong">Guangzhi Tong</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">PMC</idno>
<idno type="pmid">29052333</idno>
<idno type="pmc">6084361</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6084361</idno>
<idno type="RBID">PMC:6084361</idno>
<idno type="doi">10.1002/pmic.201700101</idno>
<date when="2017">2017</date>
<idno type="wicri:Area/Pmc/Corpus">000802</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000802</idno>
<idno type="wicri:Area/Pmc/Curation">000802</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000802</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000760</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000760</idno>
<idno type="wicri:Area/Ncbi/Merge">000889</idno>
<idno type="wicri:Area/Ncbi/Curation">000889</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000889</idno>
<idno type="wicri:doubleKey">1615-9853:2017:Qu Z:label:free:quantitative</idno>
<idno type="wicri:Area/Main/Merge">000A88</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en" level="a" type="main">Label‐Free Quantitative Proteomic Analysis of Differentially Expressed Membrane Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain</title>
<author>
<name sortKey="Qu, Zehui" sort="Qu, Zehui" uniqKey="Qu Z" first="Zehui" last="Qu">Zehui Qu</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Gao, Fei" sort="Gao, Fei" uniqKey="Gao F" first="Fei" last="Gao">Fei Gao</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Li, Liwei" sort="Li, Liwei" uniqKey="Li L" first="Liwei" last="Li">Liwei Li</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Zhang, Yujiao" sort="Zhang, Yujiao" uniqKey="Zhang Y" first="Yujiao" last="Zhang">Yujiao Zhang</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Jiang, Yifeng" sort="Jiang, Yifeng" uniqKey="Jiang Y" first="Yifeng" last="Jiang">Yifeng Jiang</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Yu, Lingxue" sort="Yu, Lingxue" uniqKey="Yu L" first="Lingxue" last="Yu">Lingxue Yu</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Zhou, Yanjun" sort="Zhou, Yanjun" uniqKey="Zhou Y" first="Yanjun" last="Zhou">Yanjun Zhou</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Zheng, Hao" sort="Zheng, Hao" uniqKey="Zheng H" first="Hao" last="Zheng">Hao Zheng</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Tong, Wu" sort="Tong, Wu" uniqKey="Tong W" first="Wu" last="Tong">Wu Tong</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Li, Guoxin" sort="Li, Guoxin" uniqKey="Li G" first="Guoxin" last="Li">Guoxin Li</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Tong, Guangzhi" sort="Tong, Guangzhi" uniqKey="Tong G" first="Guangzhi" last="Tong">Guangzhi Tong</name>
<affiliation>
<nlm:aff id="pmic12759-aff-0001"></nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="pmic12759-aff-0002"></nlm:aff>
</affiliation>
</author>
</analytic>
<series>
<title level="j">Proteomics</title>
<idno type="ISSN">1615-9853</idno>
<idno type="eISSN">1615-9861</idno>
<imprint>
<date when="2017">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc>
<textClass></textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">
<title>Abstract</title>
<p>Significant differences exist between the highly pathogenic (HP) porcine reproductive and respiratory syndrome virus (PRRSV) and its attenuated pathogenic (AP) strain in the ability to infect host cells. The mechanisms by which different virulent strains invade host cells remain relatively unknown. In this study, pulmonary alveolar macrophages (PAMs) are infected with HP‐PRRSV (HuN4) and AP‐PRRSV (HuN4‐F112) for 24 h, then harvested and subjected to label‐free quantitative MS. A total of 2849 proteins are identified, including 95 that are differentially expressed. Among them, 26 proteins are located on the membrane. The most differentially expressed proteins are involved in response to stimulus, metabolic process, and immune system process, which mainly have the function of binding and catalytic activity. Cluster of differentiation CD163, vimentin (VIM), and nmII as well as detected proteins are assessed together by string analysis, which elucidated a potentially different infection mechanism. According to the function annotations, PRRSV with different virulence may mainly differ in immunology, inflammation, immune evasion as well as cell apoptosis. This is the first attempt to explore the differential characteristics between HP‐PRRSV and its attenuated PRRSV infected PAMs focusing on membrane proteins which will be of great help to further understand the different infective mechanisms of HP‐PRRSV and AP‐PRRSV.</p>
</div>
</front>
<back>
<div1 type="bibliography">
<listBibl>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/StressCovidV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A88 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000A88 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    StressCovidV1
   |flux=    Main
   |étape=   Merge
   |type=    RBID
   |clé=     PMC:6084361
   |texte=   Label‐Free Quantitative Proteomic Analysis of Differentially Expressed Membrane Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i   -Sk "pubmed:29052333" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a StressCovidV1 

Wicri

This area was generated with Dilib version V0.6.33.
Data generation: Wed May 6 16:44:09 2020. Site generation: Sun Mar 28 08:26:57 2021