StressCovidV1, Main, Exploration, bibRecord, 001082

Evolutionarily conserved domain of heat shock transcription factor negatively regulates oligomerization and DNA binding.

Identifieur interne : 001082 ( Main/Exploration ); précédent : 001081; suivant : 001083

Evolutionarily conserved domain of heat shock transcription factor negatively regulates oligomerization and DNA binding.

Auteurs : Azumi Ota [Japon] ; Yasuaki Enoki ; Noritaka Yamamoto ; Maki Sawai ; Hiroshi Sakurai

Source :

Biochimica et biophysica acta [ 0006-3002 ] ; 2013.

RBID : pubmed:23567048

Descripteurs français

KwdFr :
- ADN (métabolisme), Biopolymères (), Facteurs de transcription (), Facteurs de transcription (métabolisme), Facteurs de transcription (physiologie), Protéines de liaison à l'ADN (), Protéines de liaison à l'ADN (métabolisme), Protéines de liaison à l'ADN (physiologie), RT-PCR, Séquence conservée, Évolution biologique.
MESH :
- métabolisme : ADN, Facteurs de transcription, Protéines de liaison à l'ADN.
- physiologie : Facteurs de transcription, Protéines de liaison à l'ADN.
- Biopolymères, Facteurs de transcription, Protéines de liaison à l'ADN, RT-PCR, Séquence conservée, Évolution biologique.

English descriptors

KwdEn :
- Biological Evolution, Biopolymers (chemistry), Conserved Sequence, DNA (metabolism), DNA-Binding Proteins (chemistry), DNA-Binding Proteins (metabolism), DNA-Binding Proteins (physiology), Heat Shock Transcription Factors, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors (chemistry), Transcription Factors (metabolism), Transcription Factors (physiology).
MESH :
- chemical , chemistry : Biopolymers, DNA-Binding Proteins, Transcription Factors.
- chemical , metabolism : DNA, DNA-Binding Proteins, Transcription Factors.
- chemical , physiology : DNA-Binding Proteins, Transcription Factors.
- Biological Evolution, Conserved Sequence, Heat Shock Transcription Factors, Reverse Transcriptase Polymerase Chain Reaction.

Abstract

Heat shock transcription factor (HSF) regulates the expression of genes encoding molecular chaperones and stress-responsive proteins. Conversion of HSF from a monomer to a homotrimer or heterotrimer is essential for its binding to heat shock elements (HSEs) comprised of inverted repeats of the pentamer nGAAn. Here, we constructed various human HSF1 derivatives and analyzed their transcriptional activity through the continuously and discontinuously arranged nGAAn units. We identified a short stretch of amino acids that inhibits the activation ability of HSF1, especially through discontinuous HSEs. This stretch is conserved in HSFs of various organisms, interacts with the hydrophobic repeat regions that mediate HSF oligomerization, and impedes homotrimer formation and DNA binding. This conserved domain plays an important role in maintaining HSF in an inactive monomeric form.

DOI: 10.1016/j.bbagrm.2013.03.011
PubMed: 23567048

Affiliations:

Japon

Le document en format XML

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<front><div type="abstract" xml:lang="en">Heat shock transcription factor (HSF) regulates the expression of genes encoding molecular chaperones and stress-responsive proteins. Conversion of HSF from a monomer to a homotrimer or heterotrimer is essential for its binding to heat shock elements (HSEs) comprised of inverted repeats of the pentamer nGAAn. Here, we constructed various human HSF1 derivatives and analyzed their transcriptional activity through the continuously and discontinuously arranged nGAAn units. We identified a short stretch of amino acids that inhibits the activation ability of HSF1, especially through discontinuous HSEs. This stretch is conserved in HSFs of various organisms, interacts with the hydrophobic repeat regions that mediate HSF oligomerization, and impedes homotrimer formation and DNA binding. This conserved domain plays an important role in maintaining HSF in an inactive monomeric form. </div>
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Serveur d'exploration Stress et Covid

Evolutionarily conserved domain of heat shock transcription factor negatively regulates oligomerization and DNA binding.

Evolutionarily conserved domain of heat shock transcription factor negatively regulates oligomerization and DNA binding.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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