N₂ gas plasma inactivates influenza virus mediated by oxidative stress.
Identifieur interne : 000F13 ( Main/Exploration ); précédent : 000F12; suivant : 000F14N₂ gas plasma inactivates influenza virus mediated by oxidative stress.
Auteurs : Akikazu Sakudo [Japon] ; Tatsuya Misawa [Japon] ; Naohiro Shimizu [Japon] ; Yuichiro Imanishi [Japon]Source :
- Frontiers in bioscience (Elite edition) [ 1945-0508 ] ; 2014.
Descripteurs français
- KwdFr :
- Azote (), Azote (pharmacologie), Chromatographie d'affinité, Désinfectants (), Désinfectants (pharmacologie), Microscopie électronique à balayage, Orthomyxoviridae (), Orthomyxoviridae (ultrastructure), Protéines de l'enveloppe virale (métabolisme), Protéolyse (), Réaction de polymérisation en chaine en temps réel, Spectrophotométrie, Spectroscopie infrarouge à transformée de Fourier, Stress oxydatif (), Technique de Western, Température, Test ELISA, Tests d'hémagglutination.
- MESH :
- métabolisme : Protéines de l'enveloppe virale.
- pharmacologie : Azote, Désinfectants.
- Azote, Chromatographie d'affinité, Désinfectants, Microscopie électronique à balayage, Orthomyxoviridae, Protéolyse, Réaction de polymérisation en chaine en temps réel, Spectrophotométrie, Spectroscopie infrarouge à transformée de Fourier, Stress oxydatif, Technique de Western, Température, Test ELISA, Tests d'hémagglutination.
English descriptors
- KwdEn :
- Blotting, Western, Chromatography, Affinity, Disinfectants (chemistry), Disinfectants (pharmacology), Enzyme-Linked Immunosorbent Assay, Hemagglutination Tests, Microscopy, Electron, Scanning, Nitrogen (chemistry), Nitrogen (pharmacology), Orthomyxoviridae (drug effects), Orthomyxoviridae (ultrastructure), Oxidative Stress (drug effects), Proteolysis (drug effects), Real-Time Polymerase Chain Reaction, Spectrophotometry, Spectroscopy, Fourier Transform Infrared, Temperature, Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Disinfectants, Nitrogen.
- chemical , metabolism : Viral Envelope Proteins.
- chemical , pharmacology : Disinfectants, Nitrogen.
- drug effects : Orthomyxoviridae, Oxidative Stress, Proteolysis.
- ultrastructure : Orthomyxoviridae.
- Blotting, Western, Chromatography, Affinity, Enzyme-Linked Immunosorbent Assay, Hemagglutination Tests, Microscopy, Electron, Scanning, Real-Time Polymerase Chain Reaction, Spectrophotometry, Spectroscopy, Fourier Transform Infrared, Temperature.
Abstract
Here we show that N₂ gas plasma, produced by applying a short high-voltage pulse using a static induction (SI) thyristor power supply inactivates influenza virus. N₂ gas plasma treatment of influenza A and B viruses induced the degradation of viral proteins, including nucleoprotein, hemagglutinin, and neuraminidase. The injury of viral RNA genome and the inactivation of hemagglutination were also observed after N₂ gas plasma treatment. These changes were possibly due to changes in the viral envelope, because modification of the lipid content was also suggested by Fourier-transformed infrared spectroscopy. At least three major mechanisms of action (heat, UV-A, and oxidative stress (i.e. hydrogen peroxide-like molecules)) were found in this system. Among them, oxidative stress appeared to be the main factor in the inactivation of influenza virus. In addition, there was an increase in the nitrotyrosine content of viral proteins, suggesting that oxidative stress produced by N₂ gas plasma generation oxidized proteins. As a result, oxidation may be the most important factor in the inactivation, degradation, and modification of influenza virus by N₂ gas plasma.
DOI: 10.2741/e692
PubMed: 24389143
Affiliations:
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xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Biometabolic Chemistry, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215</wicri:regionArea><wicri:noRegion>Okinawa 903-0215</wicri:noRegion></affiliation></author><author><name sortKey="Misawa, Tatsuya" sort="Misawa, Tatsuya" uniqKey="Misawa T" first="Tatsuya" last="Misawa">Tatsuya Misawa</name><affiliation wicri:level="1"><nlm:affiliation>Department of Electrical and Electronic Engineering, Faculty of Science and Engineering, Saga University, Saga 840-8502, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Electrical and Electronic Engineering, Faculty of Science and Engineering, Saga University, Saga 840-8502</wicri:regionArea><wicri:noRegion>Saga 840-8502</wicri:noRegion></affiliation></author><author><name sortKey="Shimizu, Naohiro" sort="Shimizu, Naohiro" uniqKey="Shimizu N" first="Naohiro" last="Shimizu">Naohiro 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Infrared</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Azote</term><term>Chromatographie d'affinité</term><term>Désinfectants</term><term>Microscopie électronique à balayage</term><term>Orthomyxoviridae</term><term>Protéolyse</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Spectrophotométrie</term><term>Spectroscopie infrarouge à transformée de Fourier</term><term>Stress oxydatif</term><term>Technique de Western</term><term>Température</term><term>Test ELISA</term><term>Tests d'hémagglutination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Here we show that N₂ gas plasma, produced by applying a short high-voltage pulse using a static induction (SI) thyristor power supply inactivates influenza virus. N₂ gas plasma treatment of influenza A and B viruses induced the degradation of viral proteins, including nucleoprotein, hemagglutinin, and neuraminidase. The injury of viral RNA genome and the inactivation of hemagglutination were also observed after N₂ gas plasma treatment. These changes were possibly due to changes in the viral envelope, because modification of the lipid content was also suggested by Fourier-transformed infrared spectroscopy. At least three major mechanisms of action (heat, UV-A, and oxidative stress (i.e. hydrogen peroxide-like molecules)) were found in this system. Among them, oxidative stress appeared to be the main factor in the inactivation of influenza virus. In addition, there was an increase in the nitrotyrosine content of viral proteins, suggesting that oxidative stress produced by N₂ gas plasma generation oxidized proteins. As a result, oxidation may be the most important factor in the inactivation, degradation, and modification of influenza virus by N₂ gas plasma. </div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Sakudo, Akikazu" sort="Sakudo, Akikazu" uniqKey="Sakudo A" first="Akikazu" last="Sakudo">Akikazu Sakudo</name></noRegion><name sortKey="Imanishi, Yuichiro" sort="Imanishi, Yuichiro" uniqKey="Imanishi Y" first="Yuichiro" last="Imanishi">Yuichiro Imanishi</name><name sortKey="Misawa, Tatsuya" sort="Misawa, Tatsuya" uniqKey="Misawa T" first="Tatsuya" last="Misawa">Tatsuya Misawa</name><name sortKey="Shimizu, Naohiro" sort="Shimizu, Naohiro" uniqKey="Shimizu N" first="Naohiro" last="Shimizu">Naohiro Shimizu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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