Characterization of the nonstructural and spike proteins of the human respiratory coronavirus OC43: comparison with bovine enteric coronavirus.
Identifieur interne : 003542 ( PubMed/Curation ); précédent : 003541; suivant : 003543Characterization of the nonstructural and spike proteins of the human respiratory coronavirus OC43: comparison with bovine enteric coronavirus.
Auteurs : S. Mounir [Canada] ; P. Labonté ; P J TalbotSource :
- Advances in experimental medicine and biology [ 0065-2598 ] ; 1993.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Cadres ouverts de lecture, Cellules cancéreuses en culture, Coronavirus (génétique), Coronavirus bovin (génétique), Coronavirus humain OC43, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Gènes viraux, Humains, Protéines de fusion recombinantes (biosynthèse), Protéines de l'enveloppe virale (génétique), Protéines virales non structurales (biosynthèse), Protéines virales non structurales (génétique), Protéines virales structurales (génétique), Spécificité d'espèce, Séquence d'acides aminés, Séquence nucléotidique, Tumeurs du rectum.
- MESH :
- biosynthèse : Protéines de fusion recombinantes, Protéines virales non structurales.
- génétique : ARN viral, Coronavirus, Coronavirus bovin, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines virales non structurales, Protéines virales structurales.
- Cadres ouverts de lecture, Cellules cancéreuses en culture, Coronavirus humain OC43, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Gènes viraux, Humains, Spécificité d'espèce, Séquence d'acides aminés, Séquence nucléotidique, Tumeurs du rectum.
English descriptors
- KwdEn :
- Amino Acid Sequence, Base Sequence, Coronavirus (genetics), Coronavirus OC43, Human, Coronavirus, Bovine (genetics), Genes, Viral, Humans, Membrane Glycoproteins (genetics), Molecular Sequence Data, Open Reading Frames, RNA, Viral (genetics), Recombinant Fusion Proteins (biosynthesis), Rectal Neoplasms, Species Specificity, Spike Glycoprotein, Coronavirus, Tumor Cells, Cultured, Viral Envelope Proteins (genetics), Viral Nonstructural Proteins (biosynthesis), Viral Nonstructural Proteins (genetics), Viral Structural Proteins (genetics).
- MESH :
- chemical , biosynthesis : Recombinant Fusion Proteins, Viral Nonstructural Proteins.
- chemical , genetics : Membrane Glycoproteins, RNA, Viral, Viral Envelope Proteins, Viral Nonstructural Proteins, Viral Structural Proteins.
- genetics : Coronavirus, Coronavirus, Bovine.
- Amino Acid Sequence, Base Sequence, Coronavirus OC43, Human, Genes, Viral, Humans, Molecular Sequence Data, Open Reading Frames, Rectal Neoplasms, Species Specificity, Spike Glycoprotein, Coronavirus, Tumor Cells, Cultured.
Abstract
The nucleotide sequence of the region between the spike (S) and the membrane (M) protein genes, and sequences of the S and ns2 genes of the OC43 strain of human coronavirus (HCV-OC43) were determined. The ns2 gene comprises an open reading frame (ORF) encoding a putative nonstructural (ns) protein of 279 amino acids with a predicted molecular mass of 32-kDa. The S gene comprises an ORF encoding a protein of 1353 amino acid residues, with a predicted molecular weight of 149,918. Sequence comparison between HCV-OC43 and the antigenically related bovine coronavirus (BCV) revealed more sequence divergence in the putative bulbous part of the S protein (S1) than in the stem region (S2). The cysteine residues near the transmembrane domain and the internal predicted protease cleavage site are conserved in the HCV-OC43 S protein. Nucleotide sequence analysis of the region between the S and M gene loci revealed the presence of an unexpected intragenomic partial leader sequence and two ORFs encoding potential proteins of 12.9 and 9.5-kDa. These two proteins were identified as nonstructural by comparison with the homologous BCV genes. In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated down-stream of the 12.9-kDa ORF, but is expressed in infected cells, as shown by immunofluorescence. Interestingly, two ORFs, potentially encoding 4.9 and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, indicating that they are not essential for viral replication in HRT-18 cells.
DOI: 10.1007/978-1-4615-2996-5_10
PubMed: 8209772
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Mounir</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Recherche en Virologie, Institut Armand-Frappier, Université du Québec, Laval, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de Recherche en Virologie, Institut Armand-Frappier, Université du Québec, Laval</wicri:regionArea></affiliation></author><author><name sortKey="Labonte, P" sort="Labonte, P" uniqKey="Labonte P" first="P" last="Labonté">P. Labonté</name></author><author><name sortKey="Talbot, P J" sort="Talbot, P J" uniqKey="Talbot P" first="P J" last="Talbot">P J Talbot</name></author></analytic><series><title level="j">Advances in experimental medicine and biology</title><idno type="ISSN">0065-2598</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Coronavirus (genetics)</term><term>Coronavirus OC43, Human</term><term>Coronavirus, Bovine (genetics)</term><term>Genes, Viral</term><term>Humans</term><term>Membrane Glycoproteins (genetics)</term><term>Molecular Sequence Data</term><term>Open Reading Frames</term><term>RNA, Viral (genetics)</term><term>Recombinant Fusion Proteins (biosynthesis)</term><term>Rectal Neoplasms</term><term>Species Specificity</term><term>Spike Glycoprotein, Coronavirus</term><term>Tumor Cells, Cultured</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Nonstructural Proteins (biosynthesis)</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Structural Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Cadres ouverts de lecture</term><term>Cellules cancéreuses en culture</term><term>Coronavirus (génétique)</term><term>Coronavirus bovin (génétique)</term><term>Coronavirus humain OC43</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Gènes viraux</term><term>Humains</term><term>Protéines de fusion recombinantes (biosynthèse)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines virales non structurales (biosynthèse)</term><term>Protéines virales non structurales (génétique)</term><term>Protéines virales structurales (génétique)</term><term>Spécificité d'espèce</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Tumeurs du rectum</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Recombinant Fusion Proteins</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>RNA, Viral</term><term>Viral Envelope Proteins</term><term>Viral Nonstructural Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines de fusion recombinantes</term><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term><term>Coronavirus, Bovine</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Coronavirus</term><term>Coronavirus bovin</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines virales non structurales</term><term>Protéines virales structurales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Coronavirus OC43, Human</term><term>Genes, Viral</term><term>Humans</term><term>Molecular Sequence Data</term><term>Open Reading Frames</term><term>Rectal Neoplasms</term><term>Species Specificity</term><term>Spike Glycoprotein, Coronavirus</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Cellules cancéreuses en culture</term><term>Coronavirus humain OC43</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Gènes viraux</term><term>Humains</term><term>Spécificité d'espèce</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Tumeurs du rectum</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The nucleotide sequence of the region between the spike (S) and the membrane (M) protein genes, and sequences of the S and ns2 genes of the OC43 strain of human coronavirus (HCV-OC43) were determined. The ns2 gene comprises an open reading frame (ORF) encoding a putative nonstructural (ns) protein of 279 amino acids with a predicted molecular mass of 32-kDa. The S gene comprises an ORF encoding a protein of 1353 amino acid residues, with a predicted molecular weight of 149,918. Sequence comparison between HCV-OC43 and the antigenically related bovine coronavirus (BCV) revealed more sequence divergence in the putative bulbous part of the S protein (S1) than in the stem region (S2). The cysteine residues near the transmembrane domain and the internal predicted protease cleavage site are conserved in the HCV-OC43 S protein. Nucleotide sequence analysis of the region between the S and M gene loci revealed the presence of an unexpected intragenomic partial leader sequence and two ORFs encoding potential proteins of 12.9 and 9.5-kDa. These two proteins were identified as nonstructural by comparison with the homologous BCV genes. In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated down-stream of the 12.9-kDa ORF, but is expressed in infected cells, as shown by immunofluorescence. Interestingly, two ORFs, potentially encoding 4.9 and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, indicating that they are not essential for viral replication in HRT-18 cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8209772</PMID><DateCompleted><Year>1994</Year><Month>07</Month><Day>13</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0065-2598</ISSN><JournalIssue CitedMedium="Print"><Volume>342</Volume><PubDate><Year>1993</Year></PubDate></JournalIssue><Title>Advances in experimental medicine and biology</Title><ISOAbbreviation>Adv. Exp. Med. 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The cysteine residues near the transmembrane domain and the internal predicted protease cleavage site are conserved in the HCV-OC43 S protein. Nucleotide sequence analysis of the region between the S and M gene loci revealed the presence of an unexpected intragenomic partial leader sequence and two ORFs encoding potential proteins of 12.9 and 9.5-kDa. These two proteins were identified as nonstructural by comparison with the homologous BCV genes. In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated down-stream of the 12.9-kDa ORF, but is expressed in infected cells, as shown by immunofluorescence. 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Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Adv Exp Med Biol</MedlineTA><NlmUniqueID>0121103</NlmUniqueID><ISSNLinking>0065-2598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope 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