Structural and functional analysis of the surface protein of human coronavirus OC43.
Identifieur interne : 003538 ( PubMed/Curation ); précédent : 003537; suivant : 003539Structural and functional analysis of the surface protein of human coronavirus OC43.
Auteurs : F. Künkel [Allemagne] ; G. HerrlerSource :
- Virology [ 0042-6822 ] ; 1993.
Descripteurs français
- KwdFr :
- ADN viral, Acide N-acétyl-neuraminique, Acides sialiques (métabolisme), Animaux, Cellules cancéreuses en culture, Clonage moléculaire, Coronaviridae (génétique), Coronaviridae (métabolisme), Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Hémagglutinines virales (génétique), Hémagglutinines virales (métabolisme), Liaison aux protéines, Poulets, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Séquence d'acides aminés, Séquence nucléotidique, Tests d'agglutination.
- MESH :
- génétique : Coronaviridae, Hémagglutinines virales, Protéines de l'enveloppe virale.
- métabolisme : Acides sialiques, Coronaviridae, Hémagglutinines virales, Protéines de l'enveloppe virale.
- ADN viral, Acide N-acétyl-neuraminique, Animaux, Cellules cancéreuses en culture, Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Liaison aux protéines, Poulets, Séquence d'acides aminés, Séquence nucléotidique, Tests d'agglutination.
English descriptors
- KwdEn :
- Agglutination Tests, Amino Acid Sequence, Animals, Base Sequence, Chickens, Cloning, Molecular, Coronaviridae (genetics), Coronaviridae (metabolism), DNA, Viral, Hemagglutinins, Viral (genetics), Hemagglutinins, Viral (metabolism), Humans, Membrane Glycoproteins, Molecular Sequence Data, N-Acetylneuraminic Acid, Protein Binding, Sialic Acids (metabolism), Spike Glycoprotein, Coronavirus, Tumor Cells, Cultured, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , genetics : Hemagglutinins, Viral, Viral Envelope Proteins.
- chemical , metabolism : Hemagglutinins, Viral, Sialic Acids, Viral Envelope Proteins.
- chemical : DNA, Viral, Membrane Glycoproteins, N-Acetylneuraminic Acid, Spike Glycoprotein, Coronavirus.
- genetics : Coronaviridae.
- metabolism : Coronaviridae.
- Agglutination Tests, Amino Acid Sequence, Animals, Base Sequence, Chickens, Cloning, Molecular, Humans, Molecular Sequence Data, Protein Binding, Tumor Cells, Cultured.
Abstract
The two surface glycoproteins S and HE of human coronavirus OC43 (HCV-OC43) were isolated from the viral membrane and purified. Only the S protein was able to agglutinate chicken erythrocytes, indicating that this viral protein is the major hemagglutinin of HCV-OC43. The receptor determinant recognized by this virus on the surface of erythrocytes is N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) which is also used by bovine coronavirus for attachment to cells. By analyzing erythrocytes containing different amounts of Neu5,9Ac2 in either of two linkage types, it was found that there are subtle differences in the affinity of both viruses for 9-O-acetylated sialic acid. Bovine coronavirus was more efficient in recognizing low amounts of Neu5,9Ac2 alpha 2,3 linked to galactose, whereas HCV-OC43 was superior with respect to the alpha 2,6 linkage. The gene coding for the S protein of HCV-OC43 was cloned and sequenced. A large open reading frame predicts a polypeptide of 150 kDa in the unglycosylated form. A protein of about 190 kDa is expected if the 20 potential glycosylation sites are used for attachment of N-linked oligosaccharide side chains. These predictions were confirmed by in vitro transcription and translation of the gene in the presence or absence of canine pancreatic microsomal membranes. A high degree of sequence homology was found between the S proteins of HCV-OC43 and bovine coronavirus. Structural and functional analyses of more strains should help to identify the location of the sialic acid-binding site.
DOI: 10.1006/viro.1993.1360
PubMed: 8317096
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Künkel</name><affiliation wicri:level="1"><nlm:affiliation>Institut für Virologie, Philipps-Universität Marburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Virologie, Philipps-Universität Marburg</wicri:regionArea></affiliation></author><author><name sortKey="Herrler, G" sort="Herrler, G" uniqKey="Herrler G" first="G" last="Herrler">G. 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Only the S protein was able to agglutinate chicken erythrocytes, indicating that this viral protein is the major hemagglutinin of HCV-OC43. The receptor determinant recognized by this virus on the surface of erythrocytes is N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) which is also used by bovine coronavirus for attachment to cells. By analyzing erythrocytes containing different amounts of Neu5,9Ac2 in either of two linkage types, it was found that there are subtle differences in the affinity of both viruses for 9-O-acetylated sialic acid. Bovine coronavirus was more efficient in recognizing low amounts of Neu5,9Ac2 alpha 2,3 linked to galactose, whereas HCV-OC43 was superior with respect to the alpha 2,6 linkage. The gene coding for the S protein of HCV-OC43 was cloned and sequenced. A large open reading frame predicts a polypeptide of 150 kDa in the unglycosylated form. A protein of about 190 kDa is expected if the 20 potential glycosylation sites are used for attachment of N-linked oligosaccharide side chains. These predictions were confirmed by in vitro transcription and translation of the gene in the presence or absence of canine pancreatic microsomal membranes. A high degree of sequence homology was found between the S proteins of HCV-OC43 and bovine coronavirus. Structural and functional analyses of more strains should help to identify the location of the sialic acid-binding site.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8317096</PMID><DateCompleted><Year>1993</Year><Month>07</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0042-6822</ISSN><JournalIssue CitedMedium="Print"><Volume>195</Volume><Issue>1</Issue><PubDate><Year>1993</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Virology</Title><ISOAbbreviation>Virology</ISOAbbreviation></Journal><ArticleTitle>Structural and functional analysis of the surface protein of human coronavirus OC43.</ArticleTitle><Pagination><MedlinePgn>195-202</MedlinePgn></Pagination><Abstract><AbstractText>The two surface glycoproteins S and HE of human coronavirus OC43 (HCV-OC43) were isolated from the viral membrane and purified. Only the S protein was able to agglutinate chicken erythrocytes, indicating that this viral protein is the major hemagglutinin of HCV-OC43. The receptor determinant recognized by this virus on the surface of erythrocytes is N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) which is also used by bovine coronavirus for attachment to cells. By analyzing erythrocytes containing different amounts of Neu5,9Ac2 in either of two linkage types, it was found that there are subtle differences in the affinity of both viruses for 9-O-acetylated sialic acid. Bovine coronavirus was more efficient in recognizing low amounts of Neu5,9Ac2 alpha 2,3 linked to galactose, whereas HCV-OC43 was superior with respect to the alpha 2,6 linkage. The gene coding for the S protein of HCV-OC43 was cloned and sequenced. A large open reading frame predicts a polypeptide of 150 kDa in the unglycosylated form. A protein of about 190 kDa is expected if the 20 potential glycosylation sites are used for attachment of N-linked oligosaccharide side chains. These predictions were confirmed by in vitro transcription and translation of the gene in the presence or absence of canine pancreatic microsomal membranes. A high degree of sequence homology was found between the S proteins of HCV-OC43 and bovine coronavirus. Structural and functional analyses of more strains should help to identify the location of the sialic acid-binding site.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Künkel</LastName><ForeName>F</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Institut für Virologie, Philipps-Universität Marburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Herrler</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Virology</MedlineTA><NlmUniqueID>0110674</NlmUniqueID><ISSNLinking>0042-6822</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, 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MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006389" MajorTopicYN="N">Hemagglutinins, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="Y">Membrane Glycoproteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019158" MajorTopicYN="N">N-Acetylneuraminic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012794" 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